Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,754,950 T→A 100% I367F (ATT→TTT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,754,9500TA99.5% 863.1 / ‑5.6 274I367F (ATT→TTT) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (140/128);  minor base C (0/5);  total (141/133)
Fisher's exact test for biased strand distribution p-value = 2.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754804‑1755093
                                                                                                                                                  |                                                                                                                                               
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGACTATCTGTTCATCATCAAAAc                                                                                                                                               >  2:3850584/1‑149 (MQ=255)
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                               >  2:2228906/1‑149 (MQ=255)
aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                               <  2:4076861/149‑1 (MQ=255)
     ttCGGGCCAGCACCTGCTTTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcccc                                                                                                                                          <  1:2103688/149‑1 (MQ=255)
     ttCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcccc                                                                                                                                          >  1:2960839/1‑149 (MQ=255)
       cGGGCCAGCACCTGCTTTTTAAGCCCATCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATATAATATCTGTTCATCATCAAAACGCCCCAc                                                                                                                                        <  1:4023169/149‑1 (MQ=255)
       cGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAc                                                                                                                                        <  1:3453130/149‑1 (MQ=255)
        gggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAcc                                                                                                                                       <  1:2200314/149‑1 (MQ=255)
        gggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAcc                                                                                                                                       >  1:1543529/1‑149 (MQ=255)
          gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGt                                                                                                                                     <  1:3065422/149‑1 (MQ=255)
             aGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTcgc                                                                                                                                  >  2:3252946/1‑149 (MQ=255)
                  ctgctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGt                                                                                                                             <  2:1176568/149‑1 (MQ=255)
                     ctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCGGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGc                                                                                                                          <  2:3331745/149‑1 (MQ=255)
                          tAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAt                                                                                                                     <  1:3495916/149‑1 (MQ=255)
                          tAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAt                                                                                                                     <  2:4573530/149‑1 (MQ=255)
                            aGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAttt                                                                                                                   <  2:1608711/149‑1 (MQ=255)
                            aGCCCCTCATCACGCAGTGCGTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGATTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAttt                                                                                                                   <  2:3996692/149‑1 (MQ=255)
                               cccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcac                                                                                                                >  2:1116947/1‑149 (MQ=255)
                                  tcatcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatc                                                                                                             <  2:4259216/149‑1 (MQ=255)
                                    atcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatcat                                                                                                           <  2:1101795/149‑1 (MQ=255)
                                         gCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCa                                                                                                      <  2:2696172/149‑1 (MQ=255)
                                            gTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAg                                                                                                   >  1:1861225/1‑149 (MQ=255)
                                                ttCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGAttt                                                                                               <  2:2571492/149‑1 (MQ=255)
                                                 tCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTc                                                                                              <  1:3141686/149‑1 (MQ=255)
                                                  ccGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTcc                                                                                             <  2:2356859/149‑1 (MQ=255)
                                                    gTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                           >  2:2097234/1‑149 (MQ=255)
                                                    gTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                           <  2:219414/149‑1 (MQ=255)
                                                      cAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCt                                                                                         <  1:1581799/149‑1 (MQ=255)
                                                       aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTg                                                                                        <  2:4480837/149‑1 (MQ=255)
                                                       aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTg                                                                                        >  1:4724653/1‑149 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       >  1:1990824/1‑149 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       <  2:3439636/149‑1 (MQ=255)
                                                        gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                       >  2:2845556/1‑149 (MQ=255)
                                                         ttCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCg                                                                                      >  2:4378485/1‑149 (MQ=255)
                                                          tCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCgg                                                                                     <  1:3217159/149‑1 (MQ=255)
                                                             gCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:2258537/1‑149 (MQ=255)
                                                             gCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:263673/1‑149 (MQ=255)
                                                              cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTc                                                                                 >  2:1466066/1‑149 (MQ=255)
                                                              cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTc                                                                                 <  2:1299345/149‑1 (MQ=255)
                                                               aGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCg                                                                                <  1:4283742/149‑1 (MQ=255)
                                                                   aaGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATc                                                                            <  1:180492/149‑1 (MQ=255)
                                                                    aGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATcc                                                                           >  2:1998708/1‑149 (MQ=255)
                                                                      gCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCAc                                                                         <  2:3418715/149‑1 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      >  2:3940107/1‑149 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      <  2:2079531/149‑1 (MQ=255)
                                                                         tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                      >  1:3404023/1‑149 (MQ=255)
                                                                            aTGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGc                                                                   <  2:3612112/149‑1 (MQ=255)
                                                                             tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGcc                                                                  >  2:2196378/1‑149 (MQ=255)
                                                                                tcatcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGt                                                               <  2:2186759/149‑1 (MQ=255)
                                                                                 catcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGtt                                                              <  1:874999/149‑1 (MQ=255)
                                                                                   tcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTg                                                            >  2:170277/1‑149 (MQ=255)
                                                                                     tgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGcc                                                          <  1:974190/149‑1 (MQ=255)
                                                                                     tgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGcc                                                          >  2:710428/1‑149 (MQ=255)
                                                                                        tgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCAt                                                       <  1:1346421/149‑1 (MQ=255)
                                                                                         gggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                      <  1:3013592/149‑1 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     >  2:2383029/1‑149 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     <  1:2539990/149‑1 (MQ=255)
                                                                                          ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                     <  2:535883/149‑1 (MQ=255)
                                                                                            cATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATcagc                                                   <  1:3670532/149‑1 (MQ=255)
                                                                                                ctgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAt                                               <  2:1543529/149‑1 (MQ=255)
                                                                                                 tgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAtt                                              <  1:2844246/149‑1 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           >  2:4712825/1‑149 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           <  1:1579467/149‑1 (MQ=255)
                                                                                                    tgattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           >  2:847396/1‑149 (MQ=255)
                                                                                                     gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttg                                          <  1:4346870/149‑1 (MQ=255)
                                                                                                     gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttg                                          >  2:1884825/1‑149 (MQ=255)
                                                                                                         gatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTgg                                      >  2:2766324/1‑149 (MQ=255)
                                                                                                         gatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTgg                                      >  2:2494554/1‑149 (MQ=255)
                                                                                                          atCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGt                                     <  1:2378274/149‑1 (MQ=255)
                                                                                                           tCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTc                                    <  1:55527/149‑1 (MQ=255)
                                                                                                              cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATc                                 >  2:1067671/1‑149 (MQ=255)
                                                                                                              cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATc                                 <  1:3792610/149‑1 (MQ=255)
                                                                                                               gcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCt                                >  1:4760947/1‑149 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCCTCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  1:3876432/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  2:1388619/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              <  1:753614/149‑1 (MQ=255)
                                                                                                                 gcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGa                              >  1:257546/1‑149 (MQ=255)
                                                                                                                    aGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCa                           >  1:447464/1‑149 (MQ=255)
                                                                                                                    aGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCa                           >  1:4201367/1‑149 (MQ=255)
                                                                                                                     gcgcAGATAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATGTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          <  1:1460737/149‑1 (MQ=255)
                                                                                                                     gcgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          <  2:960612/149‑1 (MQ=255)
                                                                                                                      cgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATc                         >  2:4540909/1‑149 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        <  1:1966554/149‑1 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        >  2:2545196/1‑149 (MQ=255)
                                                                                                                       gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                        >  2:2958715/1‑149 (MQ=255)
                                                                                                                         aGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGt                      >  2:2649140/1‑149 (MQ=255)
                                                                                                                            tAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtttt                   <  1:2026430/149‑1 (MQ=255)
                                                                                                                            tAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtttt                   >  2:1137173/1‑149 (MQ=255)
                                                                                                                              gAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 <  2:2699145/149‑1 (MQ=255)
                                                                                                                              gAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 >  1:692743/1‑149 (MQ=255)
                                                                                                                                     tGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtg          >  1:3647740/1‑149 (MQ=255)
                                                                                                                                     tGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtg          >  2:1394508/1‑149 (MQ=255)
                                                                                                                                      gTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtgc         >  1:705641/1‑149 (MQ=255)
                                                                                                                                       ttCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACCTCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGtgct        <  2:3695500/149‑1 (MQ=255)
                                                                                                                                          atcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg     >  1:60858/1‑149 (MQ=255)
                                                                                                                                          atcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg     >  2:3811312/1‑149 (MQ=255)
                                                                                                                                           tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcgc    >  1:4619037/1‑149 (MQ=255)
                                                                                                                                           tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcgc    >  1:2437642/1‑149 (MQ=255)
                                                                                                                                             atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGa  >  1:3257457/1‑149 (MQ=255)
                                                                                                                                             atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGa  >  2:3463435/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754804‑1755093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTG  >  CP009273/1754804‑1755098
                                                                                                                                                  |                                                                                                                                                    
ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACG                                                                                                                                                   <  SRR19973071.955938/150‑1 (MQ=60)
  CGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCC                                                                                                                                                 <  SRR19973071.1782203/150‑1 (MQ=60)
    ATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCC                                                                                                                                               >  SRR19973071.2971441/1‑150 (MQ=60)
    ATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCC                                                                                                                                               >  SRR19973071.2971580/1‑150 (MQ=60)
    ATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCC                                                                                                                                               >  SRR19973071.572706/1‑150 (MQ=60)
     TTCGGGCCAGCACCTGCTTTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCA                                                                                                                                              <  SRR19973071.2111444/150‑1 (MQ=60)
       CGGGCCAGCACCTGCTTTTTAAGCCCATCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATATAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            <  SRR19973071.4037319/150‑1 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            >  SRR19973071.1549054/1‑150 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            >  SRR19973071.1548970/1‑150 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            >  SRR19973071.2608519/1‑150 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            <  SRR19973071.3465315/150‑1 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            >  SRR19973071.4487671/1‑150 (MQ=60)
       CGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACC                                                                                                                                            <  SRR19973071.3465969/150‑1 (MQ=60)
        GGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCG                                                                                                                                           <  SRR19973071.4613327/150‑1 (MQ=60)
        GGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCG                                                                                                                                           <  SRR19973071.2208272/150‑1 (MQ=60)
        GGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCG                                                                                                                                           <  SRR19973071.2208454/150‑1 (MQ=60)
          GCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTC                                                                                                                                         <  SRR19973071.3076292/150‑1 (MQ=60)
          GCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTC                                                                                                                                         <  SRR19973071.3076279/150‑1 (MQ=60)
           CCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATTGAATATCTGTTCATCATCAAAACGCCCCACCGTCG                                                                                                                                        <  SRR19973071.2429714/150‑1 (MQ=60)
           CCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCG                                                                                                                                        <  SRR19973071.2429328/150‑1 (MQ=60)
               CACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCC                                                                                                                                    >  SRR19973071.629231/1‑150 (MQ=60)
                  CTGCTGTTTAAGCCCCTCACCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTG                                                                                                                                 <  SRR19973071.1905805/150‑1 (MQ=60)
                     CTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCT                                                                                                                              <  SRR19973071.2085153/150‑1 (MQ=60)
                     CTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCT                                                                                                                              <  SRR19973071.2085718/150‑1 (MQ=60)
                      TGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTG                                                                                                                             >  SRR19973071.4247396/1‑150 (MQ=60)
                          TAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATT                                                                                                                         <  SRR19973071.3508201/150‑1 (MQ=60)
                             GCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCA                                                                                                                      <  SRR19973071.159106/150‑1 (MQ=60)
                             GCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCA                                                                                                                      <  SRR19973071.1586356/150‑1 (MQ=60)
                             GCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCA                                                                                                                      <  SRR19973071.2326421/150‑1 (MQ=60)
                               CCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACA                                                                                                                    >  SRR19973071.1567552/1‑150 (MQ=60)
                                CCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACAT                                                                                                                   >  SRR19973071.703848/1‑150 (MQ=60)
                                  TCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCA                                                                                                                 >  SRR19973071.405140/1‑150 (MQ=60)
                                      CACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTG                                                                                                             <  SRR19973071.3239193/150‑1 (MQ=60)
                                        CGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCTTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCA                                                                                                           <  SRR19973071.36269/150‑1 (MQ=60)
                                        CGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCA                                                                                                           <  SRR19973071.35619/150‑1 (MQ=60)
                                         GCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCAT                                                                                                          <  SRR19973071.200455/150‑1 (MQ=60)
                                         GCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCAT                                                                                                          <  SRR19973071.200490/150‑1 (MQ=60)
                                           AGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAG                                                                                                        >  SRR19973071.1868103/1‑150 (MQ=60)
                                           AGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAG                                                                                                        >  SRR19973071.1867913/1‑150 (MQ=60)
                                                 TCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCC                                                                                                  <  SRR19973071.3152701/150‑1 (MQ=60)
                                                  CCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCA                                                                                                 <  SRR19973071.550271/150‑1 (MQ=60)
                                                  CCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCA                                                                                                 >  SRR19973071.651664/1‑150 (MQ=60)
                                                  CCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCA                                                                                                 <  SRR19973071.419111/150‑1 (MQ=60)
                                                    GTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGC                                                                                               >  SRR19973071.3664789/1‑150 (MQ=60)
                                                     TCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCT                                                                                              <  SRR19973071.514500/150‑1 (MQ=60)
                                                      CAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTG                                                                                             >  SRR19973071.4741394/1‑150 (MQ=60)
                                                      CAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTG                                                                                             <  SRR19973071.1587404/150‑1 (MQ=60)
                                                      CAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTG                                                                                             <  SRR19973071.3188709/150‑1 (MQ=60)
                                                       AGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGC                                                                                            >  SRR19973071.1998009/1‑150 (MQ=60)
                                                          TCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGT                                                                                         <  SRR19973071.3228449/150‑1 (MQ=60)
                                                            GGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTT                                                                                       >  SRR19973071.2266854/1‑150 (MQ=60)
                                                            GGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTT                                                                                       >  SRR19973071.896580/1‑150 (MQ=60)
                                                            GGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTT                                                                                       >  SRR19973071.264652/1‑150 (MQ=60)
                                                               AGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGT                                                                                    <  SRR19973071.4298910/150‑1 (MQ=60)
                                                                 CGAAGGCGTAAATGATCATCTGCTGGGCATACTGCTGATTGATCCCGCTCGAGCGCATATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTAT                                                                                  <  SRR19973071.3462858/150‑1 (MQ=60)
                                                                  GAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATC                                                                                 >  SRR19973071.3173239/1‑150 (MQ=60)
                                                                   AAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCC                                                                                <  SRR19973071.4581214/150‑1 (MQ=60)
                                                                   AAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCC                                                                                <  SRR19973071.2174009/150‑1 (MQ=60)
                                                                   AAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCC                                                                                >  SRR19973071.1965854/1‑150 (MQ=60)
                                                                   AAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCC                                                                                <  SRR19973071.181249/150‑1 (MQ=60)
                                                                        GTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTC                                                                           >  SRR19973071.3416080/1‑150 (MQ=60)
                                                                        GTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTC                                                                           >  SRR19973071.3416632/1‑150 (MQ=60)
                                                                         TAAATGTTCATCTGCTGGTCATCCTGCTGATTTATCCCGCGCGAGCTCAGATATATTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCC                                                                          <  SRR19973071.2450408/150‑1 (MQ=60)
                                                                         TAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCC                                                                          <  SRR19973071.788617/150‑1 (MQ=60)
                                                                         TAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCC                                                                          <  SRR19973071.788708/150‑1 (MQ=60)
                                                                         TAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCC                                                                          <  SRR19973071.3898348/150‑1 (MQ=60)
                                                                          AAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCG                                                                         >  SRR19973071.2735436/1‑150 (MQ=60)
                                                                           AATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGC                                                                        <  SRR19973071.2744670/150‑1 (MQ=60)
                                                                             TGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCA                                                                      >  SRR19973071.1573490/1‑150 (MQ=60)
                                                                                 CATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTT                                                                  <  SRR19973071.878128/150‑1 (MQ=60)
                                                                                     TGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCC                                                              <  SRR19973071.977729/150‑1 (MQ=60)
                                                                                        TGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATC                                                           <  SRR19973071.1351375/150‑1 (MQ=60)
                                                                                         GGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCA                                                          <  SRR19973071.3024433/150‑1 (MQ=60)
                                                                                         GGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCA                                                          <  SRR19973071.3024345/150‑1 (MQ=60)
                                                                                          GGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAG                                                         <  SRR19973071.2549226/150‑1 (MQ=60)
                                                                                          GGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAG                                                         <  SRR19973071.2549231/150‑1 (MQ=60)
                                                                                            CATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCA                                                       <  SRR19973071.3683491/150‑1 (MQ=60)
                                                                                                 TGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTG                                                  <  SRR19973071.2854357/150‑1 (MQ=60)
                                                                                                    TGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTG                                               <  SRR19973071.1585063/150‑1 (MQ=60)
                                                                                                    TGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTG                                               >  SRR19973071.1531249/1‑150 (MQ=60)
                                                                                                    TGATTGATCCCGCGCGAGCGCAGATAGAATATATGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTG                                               >  SRR19973071.3928627/1‑150 (MQ=60)
                                                                                                     GATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGT                                              <  SRR19973071.4322986/150‑1 (MQ=60)
                                                                                                     GATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGT                                              <  SRR19973071.4362192/150‑1 (MQ=60)
                                                                                                     GATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGT                                              >  SRR19973071.2965182/1‑150 (MQ=60)
                                                                                                     GACTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGT                                              >  SRR19973071.2965185/1‑150 (MQ=60)
                                                                                                        TGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGG                                           <  SRR19973071.3090712/150‑1 (MQ=60)
                                                                                                          ATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTC                                         <  SRR19973071.46598/150‑1 (MQ=60)
                                                                                                          ATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTC                                         <  SRR19973071.2386853/150‑1 (MQ=60)
                                                                                                           TCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCA                                        <  SRR19973071.55847/150‑1 (MQ=60)
                                                                                                             CCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATC                                      >  SRR19973071.3772220/1‑150 (MQ=60)
                                                                                                              CGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCT                                     >  SRR19973071.2372354/1‑150 (MQ=60)
                                                                                                              CGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCT                                     >  SRR19973071.4777772/1‑150 (MQ=60)
                                                                                                              CGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCT                                     >  SRR19973071.4777689/1‑150 (MQ=60)
                                                                                                              CGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCT                                     <  SRR19973071.3806087/150‑1 (MQ=60)
                                                                                                                CGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGA                                   >  SRR19973071.258513/1‑150 (MQ=60)
                                                                                                                 GCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCCTCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGAC                                  <  SRR19973071.3890140/150‑1 (MQ=60)
                                                                                                                 GCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGAC                                  <  SRR19973071.756194/150‑1 (MQ=60)
                                                                                                                  CGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGACTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACC                                 >  SRR19973071.4223947/1‑150 (MQ=60)
                                                                                                                   GAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCA                                >  SRR19973071.449066/1‑150 (MQ=60)
                                                                                                                   GAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCA                                >  SRR19973071.449039/1‑150 (MQ=60)
                                                                                                                   GAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCA                                >  SRR19973071.4216321/1‑150 (MQ=60)
                                                                                                                    AGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAT                               >  SRR19973071.2518465/1‑150 (MQ=60)
                                                                                                                     GCGCAGATAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATGTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATC                              <  SRR19973071.1466071/150‑1 (MQ=60)
                                                                                                                      CGCAGATAGAATATCTGTTCATCATCACAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCC                             <  SRR19973071.2644906/150‑1 (MQ=60)
                                                                                                                      CGCAGATAGAATATCTGTTCACCATCAACCCGCCCCTCCGTCGCGCCGTGGCGTCATTTCACATCCTCTTCATAGATTTACAGCTGCGGTTTCGTATCCACTACCGCCATTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCC                             <  SRR19973071.4657136/150‑1 (MQ=60)
                                                                                                                       GCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCG                            <  SRR19973071.1973687/150‑1 (MQ=60)
                                                                                                                       GCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCG                            <  SRR19973071.1973775/150‑1 (MQ=60)
                                                                                                                            tgctcttccgatctATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTG                       <  SRR19973071.1439746/136‑1 (MQ=60)
                                                                                                                            TAGAATATCTGTTCATCATCAACACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTG                       <  SRR19973071.2033807/150‑1 (MQ=60)
                                                                                                                             AGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGA                      >  SRR19973071.695199/1‑150 (MQ=60)
                                                                                                                              GAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGAT                     <  SRR19973071.3360803/150‑1 (MQ=60)
                                                                                                                                ATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGG                   >  SRR19973071.559528/1‑150 (MQ=60)
                                                                                                                                ATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGG                   <  SRR19973071.3678448/150‑1 (MQ=60)
                                                                                                                                ATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGG                   >  SRR19973071.755667/1‑150 (MQ=60)
                                                                                                                                    CTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTG               >  SRR19973071.3660573/1‑150 (MQ=60)
                                                                                                                                     TGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGC              >  SRR19973071.3108288/1‑150 (MQ=60)
                                                                                                                                     TGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGC              >  SRR19973071.708117/1‑150 (MQ=60)
                                                                                                                                      GTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCT             <  SRR19973071.3864227/150‑1 (MQ=60)
                                                                                                                                      GTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCT             <  SRR19973071.2237133/150‑1 (MQ=60)
                                                                                                                                        TCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGC           >  SRR19973071.3779209/1‑150 (MQ=60)
                                                                                                                                         CATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCG          >  SRR19973071.61199/1‑150 (MQ=60)
                                                                                                                                         CATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCG          >  SRR19973071.731059/1‑150 (MQ=60)
                                                                                                                                          ATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGC         >  SRR19973071.4635505/1‑150 (MQ=60)
                                                                                                                                          ATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGC         >  SRR19973071.2446485/1‑150 (MQ=60)
                                                                                                                                           TCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCG        >  SRR19973071.185075/1‑150 (MQ=60)
                                                                                                                                            CATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA       >  SRR19973071.3268977/1‑150 (MQ=60)
                                                                                                                                            CATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA       >  SRR19973071.3268966/1‑150 (MQ=60)
                                                                                                                                                AAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTT   <  SRR19973071.171003/150‑1 (MQ=60)
                                                                                                                                                AAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTT   <  SRR19973071.1871927/150‑1 (MQ=60)
                                                                                                                                                AAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTT   >  SRR19973071.1028057/1‑150 (MQ=60)
                                                                                                                                                 AAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTG  <  SRR19973071.285209/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
ACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTG  >  CP009273/1754804‑1755098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑