Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,152,444 C→A 90.5% A193E (GCG→GAG)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,152,4440CA90.5% 720.0 / 70.2 263A193E (GCG→GAG) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (19/6);  new base A (122/116);  total (141/122)
Fisher's exact test for biased strand distribution p-value = 2.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 271 total aligned reads displayed.

TGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCA  >  CP009273/2152297‑2152591
                                                                                                                                                   |                                                                                                                                                   
tGACGTCCGATACTTGTTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgcg                                                                                                                                                    >  1:624165/1‑149 (MQ=255)
 gACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                   <  2:935602/149‑1 (MQ=255)
 gACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                   >  1:2551806/1‑149 (MQ=255)
 gACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                   >  1:1488733/1‑149 (MQ=255)
  aCGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCt                                                                                                                                                  <  2:13954/149‑1 (MQ=255)
    gTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGt                                                                                                                                                >  2:536141/1‑149 (MQ=255)
    gTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGt                                                                                                                                                >  1:955562/1‑149 (MQ=255)
     tCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGtt                                                                                                                                               >  2:3150814/1‑149 (MQ=255)
      ccGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGttt                                                                                                                                              <  1:1111583/149‑1 (MQ=255)
       cGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTa                                                                                                                                             <  1:4253168/149‑1 (MQ=255)
        gATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTaa                                                                                                                                            >  2:1880345/1‑149 (MQ=255)
         aTACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAAt                                                                                                                                           >  2:738384/1‑149 (MQ=255)
         aTACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAAt                                                                                                                                           >  2:2325604/1‑149 (MQ=255)
            cTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAg                                                                                                                                        >  1:2005888/1‑149 (MQ=255)
            cTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAg                                                                                                                                        >  2:864750/1‑149 (MQ=255)
             ttATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAgg                                                                                                                                       >  1:1053309/1‑149 (MQ=255)
              tATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAggg                                                                                                                                      >  1:1983322/1‑149 (MQ=255)
                ttCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGATGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCg                                                                                                                                    <  1:2318931/149‑1 (MQ=255)
                  cGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCgtg                                                                                                                                  <  1:1784243/149‑1 (MQ=255)
                      gggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGc                                                                                                                              <  1:2438354/149‑1 (MQ=255)
                       ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                             <  2:2005809/149‑1 (MQ=255)
                       ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                             <  2:650887/149‑1 (MQ=255)
                       ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                             <  2:3748329/149‑1 (MQ=255)
                          gAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGga                                                                                                                          >  1:4502942/1‑149 (MQ=255)
                          gAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGga                                                                                                                          >  1:4502841/1‑149 (MQ=255)
                           aaCTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGgac                                                                                                                         >  2:3081799/1‑149 (MQ=255)
                             atGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCACGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGgacga                                                                                                                       >  1:1583835/2‑149 (MQ=255)
                                  cGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTAc                                                                                                                  >  2:76280/1‑149 (MQ=255)
                                    aTTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGc                                                                                                                >  2:1635961/1‑149 (MQ=255)
                                     tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                               <  2:1235322/149‑1 (MQ=255)
                                     tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                               >  2:3876362/1‑149 (MQ=255)
                                     tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                               <  1:4647109/149‑1 (MQ=255)
                                      ttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAc                                                                                                              <  1:4606353/149‑1 (MQ=255)
                                             cGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTc                                                                                                       >  2:2776830/1‑149 (MQ=255)
                                             cGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTc                                                                                                       <  1:1127773/149‑1 (MQ=255)
                                               aCGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAg                                                                                                     >  1:357881/1‑149 (MQ=255)
                                               aCGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAg                                                                                                     >  1:1703276/1‑149 (MQ=255)
                                                cGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGc                                                                                                    <  2:272716/149‑1 (MQ=255)
                                                cGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGc                                                                                                    >  2:2417026/1‑149 (MQ=255)
                                                  cAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCATACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCaa                                                                                                  <  2:2136406/149‑1 (MQ=255)
                                                   aGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAAt                                                                                                 >  2:4746194/1‑149 (MQ=255)
                                                    gCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATg                                                                                                >  1:940846/1‑149 (MQ=255)
                                                    gCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATg                                                                                                >  2:1264432/1‑149 (MQ=255)
                                                       ggCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCa                                                                                             >  1:3215220/1‑149 (MQ=255)
                                                        gCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCaa                                                                                            >  2:2595153/1‑149 (MQ=255)
                                                        gCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCaa                                                                                            >  2:203060/1‑149 (MQ=255)
                                                             gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGc                                                                                       <  1:4571257/149‑1 (MQ=255)
                                                             gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGc                                                                                       <  1:798554/149‑1 (MQ=255)
                                                                gaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTa                                                                                    >  2:1719534/1‑149 (MQ=255)
                                                                  tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                  <  2:1530961/149‑1 (MQ=255)
                                                                  tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                  >  2:1465276/1‑149 (MQ=255)
                                                                  tttAGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                  <  2:3458910/149‑1 (MQ=255)
                                                                  tgtCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                  <  2:3919516/147‑1 (MQ=255)
                                                                   ttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAAc                                                                                 <  1:3550839/149‑1 (MQ=255)
                                                                         aaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAgg                                                                           >  1:3085852/1‑149 (MQ=255)
                                                                           tCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGgc                                                                         >  2:1079522/1‑149 (MQ=255)
                                                                                ggTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCt                                                                    <  1:4116370/149‑1 (MQ=255)
                                                                                ggTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCt                                                                    >  1:4187372/1‑149 (MQ=255)
                                                                                 gtgtTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTg                                                                   >  1:344829/1‑149 (MQ=255)
                                                                                 gtgtTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTg                                                                   >  1:898329/1‑149 (MQ=255)
                                                                                      ggTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGa                                                              <  2:344822/149‑1 (MQ=255)
                                                                                        tGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATg                                                            <  2:2227650/149‑1 (MQ=255)
                                                                                         gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATgg                                                           <  1:1363453/149‑1 (MQ=255)
                                                                                            gtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCAc                                                        <  1:3869567/149‑1 (MQ=255)
                                                                                              cgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTc                                                      <  2:413955/149‑1 (MQ=255)
                                                                                              cgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTc                                                      <  1:1325470/149‑1 (MQ=255)
                                                                                               gatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCa                                                     <  2:3809117/149‑1 (MQ=255)
                                                                                                      gAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTgg                                              >  2:1773341/1‑149 (MQ=255)
                                                                                                      gAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTgg                                              >  1:625816/1‑149 (MQ=255)
                                                                                                       aGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGc                                             <  2:3587362/149‑1 (MQ=255)
                                                                                                       aGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGc                                             >  2:282698/1‑149 (MQ=255)
                                                                                                           aGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGAt                                         >  1:3258535/1‑149 (MQ=255)
                                                                                                             cTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATcc                                       <  1:1264422/149‑1 (MQ=255)
                                                                                                              tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                      <  2:4534650/149‑1 (MQ=255)
                                                                                                              tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                      >  2:318371/1‑149 (MQ=255)
                                                                                                              tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                      >  2:319453/1‑149 (MQ=255)
                                                                                                              tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                      <  2:3623694/149‑1 (MQ=255)
                                                                                                              tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                      >  1:3000994/1‑149 (MQ=255)
                                                                                                               cACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAg                                     >  1:1423100/1‑149 (MQ=255)
                                                                                                                aCTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGa                                    >  1:2925589/1‑149 (MQ=255)
                                                                                                                 cTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCACTGGAAGATGGCACTCACCGCTGGCAGATCCAGAc                                   <  2:2937946/149‑1 (MQ=255)
                                                                                                                  tGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGAcc                                  >  2:760646/1‑149 (MQ=255)
                                                                                                                   gCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCa                                 >  1:2710638/1‑149 (MQ=255)
                                                                                                                    cccGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCaa                                <  2:2549811/149‑1 (MQ=255)
                                                                                                                    cccGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCaa                                <  2:4379376/149‑1 (MQ=255)
                                                                                                                        ccgTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAatgat                            >  1:1650181/1‑149 (MQ=255)
                                                                                                                          gTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAAtgatga                          <  2:4456924/149‑1 (MQ=255)
                                                                                                                          gTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAAtgatga                          <  2:811595/149‑1 (MQ=255)
                                                                                                                            aCGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGc                        <  2:243873/149‑1 (MQ=255)
                                                                                                                             cgcgTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCt                       <  2:1191662/149‑1 (MQ=255)
                                                                                                                             cgcgTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCt                       <  2:4187372/149‑1 (MQ=255)
                                                                                                                             cgcgTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCt                       <  2:1488733/149‑1 (MQ=255)
                                                                                                                             cgcgTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCt                       <  2:3427391/149‑1 (MQ=255)
                                                                                                                                 tCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTaaaa                   >  1:3998114/1‑149 (MQ=255)
                                                                                                                                        gAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAccgccg            >  2:409422/1‑149 (MQ=255)
                                                                                                                                          aTCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCt          >  1:829166/1‑149 (MQ=255)
                                                                                                                                              gCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAat      <  1:3842224/149‑1 (MQ=255)
                                                                                                                                                aGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAATTTGGCACTCACCGCTGGCAGATCCATACCAATGATGAGCTAAAAACCGCCGCTGAatat    <  1:3803370/149‑1 (MQ=255)
                                                                                                                                                aGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAatat    >  1:1717942/1‑149 (MQ=255)
                                                                                                                                                  gcgcTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCa  >  2:3852239/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCA  >  CP009273/2152297‑2152591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATC  >  CP009273/2152296‑2152590
                                                                                                                                                    |                                                                                                                                                  
CTGACGTCCGATACTTGTTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCG                                                                                                                                                   >  SRR19973071.626484/1‑150 (MQ=60)
CTGACGTCCGATACTTGTTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCG                                                                                                                                                   >  SRR19973071.626467/1‑150 (MQ=60)
CTGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACTATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGAACGCGTCGGGCTGAATCCGCAGGAG                                                                                                                                                   >  SRR19973071.105162/1‑150 (MQ=60)
CTGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAACTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAG                                                                                                                                                   <  SRR19973071.4097815/150‑1 (MQ=60)
CTGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAG                                                                                                                                                   >  SRR19973071.1484207/1‑150 (MQ=60)
 TGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGC                                                                                                                                                  >  SRR19973071.1494133/1‑150 (MQ=60)
 TGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGC                                                                                                                                                  >  SRR19973071.2561079/1‑150 (MQ=60)
 TGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGC                                                                                                                                                  >  SRR19973071.2561093/1‑150 (MQ=60)
    CGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGT                                                                                                                                               >  SRR19973071.959034/1‑150 (MQ=60)
    CGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGT                                                                                                                                               >  SRR19973071.958676/1‑150 (MQ=60)
      TCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTT                                                                                                                                             <  SRR19973071.1554872/150‑1 (MQ=60)
       CCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTA                                                                                                                                            <  SRR19973071.1115497/150‑1 (MQ=60)
       CCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTA                                                                                                                                            <  SRR19973071.1115514/150‑1 (MQ=60)
        CGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAA                                                                                                                                           <  SRR19973071.4268262/150‑1 (MQ=60)
        CGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAA                                                                                                                                           <  SRR19973071.4268095/150‑1 (MQ=60)
          ATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATC                                                                                                                                         >  SRR19973071.3636410/1‑150 (MQ=60)
          ATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATC                                                                                                                                         >  SRR19973071.3636353/1‑150 (MQ=60)
            ACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAG                                                                                                                                       >  SRR19973071.3252426/1‑150 (MQ=60)
            ACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAG                                                                                                                                       >  SRR19973071.653261/1‑150 (MQ=60)
            ACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAG                                                                                                                                       >  SRR19973071.2013128/1‑150 (MQ=60)
            ACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAG                                                                                                                                       >  SRR19973071.2013048/1‑150 (MQ=60)
             CTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGG                                                                                                                                      <  SRR19973071.2947218/150‑1 (MQ=60)
             CTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGG                                                                                                                                      <  SRR19973071.2947466/150‑1 (MQ=60)
             CTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGG                                                                                                                                      >  SRR19973071.1057087/1‑150 (MQ=60)
              TTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGG                                                                                                                                     >  SRR19973071.1990494/1‑150 (MQ=60)
                 TTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGATGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGT                                                                                                                                  <  SRR19973071.2327380/150‑1 (MQ=60)
                   CGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGT                                                                                                                                <  SRR19973071.1790474/150‑1 (MQ=60)
                   CGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGT                                                                                                                                <  SRR19973071.1790895/150‑1 (MQ=60)
                     CAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCG                                                                                                                              >  SRR19973071.2122513/1‑150 (MQ=60)
                      AGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGC                                                                                                                             >  SRR19973071.1364647/1‑150 (MQ=60)
                      AGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGC                                                                                                                             >  SRR19973071.1364623/1‑150 (MQ=60)
                       GGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCT                                                                                                                            <  SRR19973071.2447203/150‑1 (MQ=60)
                       GGGGGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCT                                                                                                                            <  SRR19973071.2447246/150‑1 (MQ=60)
                          TGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGA                                                                                                                         >  SRR19973071.4518945/1‑150 (MQ=60)
                          TGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGA                                                                                                                         >  SRR19973071.4518844/1‑150 (MQ=60)
                            AACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACG                                                                                                                       <  SRR19973071.896025/150‑1 (MQ=60)
                             ACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGA                                                                                                                      >  SRR19973071.4793956/1‑150 (MQ=60)
                             AATGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCACGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGA                                                                                                                      >  SRR19973071.1589445/1‑150 (MQ=60)
                              CTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGAC                                                                                                                     >  SRR19973071.3871948/1‑150 (MQ=60)
                              CTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGAC                                                                                                                     >  SRR19973071.3248576/1‑150 (MQ=60)
                                 TACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTA                                                                                                                  <  SRR19973071.1887685/150‑1 (MQ=60)
                                  ACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTAC                                                                                                                 <  SRR19973071.341274/150‑1 (MQ=60)
                                      TTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAC                                                                                                             <  SRR19973071.2770925/150‑1 (MQ=60)
                                      TTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAC                                                                                                             <  SRR19973071.4663660/150‑1 (MQ=60)
                                       TTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACC                                                                                                            <  SRR19973071.4622783/150‑1 (MQ=60)
                                       TTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACC                                                                                                            <  SRR19973071.4622812/150‑1 (MQ=60)
                                              CGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCA                                                                                                     <  SRR19973071.1131726/150‑1 (MQ=60)
                                               GACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAG                                                                                                    >  SRR19973071.359040/1‑150 (MQ=60)
                                               GACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAG                                                                                                    >  SRR19973071.359038/1‑150 (MQ=60)
                                               GACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAG                                                                                                    >  SRR19973071.690113/1‑150 (MQ=60)
                                               GACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAG                                                                                                    >  SRR19973071.1709584/1‑150 (MQ=60)
                                                    AGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATG                                                                                               >  SRR19973071.944265/1‑150 (MQ=60)
                                                    AGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATG                                                                                               >  SRR19973071.4518865/1‑150 (MQ=60)
                                                      CTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCC                                                                                             >  SRR19973071.1616098/1‑150 (MQ=60)
                                                       TGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCA                                                                                            >  SRR19973071.4135890/1‑150 (MQ=60)
                                                       TGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCA                                                                                            >  SRR19973071.3226496/1‑150 (MQ=60)
                                                       TGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCA                                                                                            >  SRR19973071.3226485/1‑150 (MQ=60)
                                                            CCGACGATTTCTCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGTAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTG                                                                                       <  SRR19973071.4614629/150‑1 (MQ=60)
                                                              GACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTGCGCACCGCCGTCAGCAATGCCAACGTGCG                                                                                     <  SRR19973071.801248/150‑1 (MQ=60)
                                                              GACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCG                                                                                     <  SRR19973071.801208/150‑1 (MQ=60)
                                                              GACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCG                                                                                     <  SRR19973071.4587547/150‑1 (MQ=60)
                                                                    TTCGCAACTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACC                                                                               <  SRR19973071.3841547/150‑1 (MQ=60)
                                                                    TTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACC                                                                               <  SRR19973071.3563049/150‑1 (MQ=60)
                                                                    TTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACC                                                                               <  SRR19973071.3563228/150‑1 (MQ=60)
                                                                     TCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCG                                                                              <  SRR19973071.3402302/150‑1 (MQ=60)
                                                                         AAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGG                                                                          >  SRR19973071.3096759/1‑150 (MQ=60)
                                                                         AAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGG                                                                          >  SRR19973071.2353532/1‑150 (MQ=60)
                                                                                CGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCT                                                                   >  SRR19973071.4202271/1‑150 (MQ=60)
                                                                                 GGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTG                                                                  >  SRR19973071.901582/1‑150 (MQ=60)
                                                                                 GGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTG                                                                  <  SRR19973071.4131021/150‑1 (MQ=60)
                                                                                 GGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTG                                                                  >  SRR19973071.345964/1‑150 (MQ=60)
                                                                                 GGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTG                                                                  >  SRR19973071.901607/1‑150 (MQ=60)
                                                                                   TGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGA                                                                >  SRR19973071.1565109/1‑150 (MQ=60)
                                                                                   TGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGA                                                                <  SRR19973071.927339/150‑1 (MQ=60)
                                                                                   TGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGA                                                                <  SRR19973071.3573125/150‑1 (MQ=60)
                                                                                     TTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAG                                                              <  SRR19973071.4098204/150‑1 (MQ=60)
                                                                                      TGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGAAGGGCGCGCTGGAAGA                                                             >  SRR19973071.1148635/1‑150 (MQ=60)
                                                                                         TGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGG                                                          <  SRR19973071.3910124/150‑1 (MQ=60)
                                                                                          GATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGC                                                         <  SRR19973071.1368484/150‑1 (MQ=60)
                                                                                          GATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGC                                                         <  SRR19973071.1368837/150‑1 (MQ=60)
                                                                                             GTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACT                                                      <  SRR19973071.3883176/150‑1 (MQ=60)
                                                                                             GTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACT                                                      <  SRR19973071.3883264/150‑1 (MQ=60)
                                                                                               CGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCA                                                    <  SRR19973071.1330340/150‑1 (MQ=60)
                                                                                                GATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCAC                                                   >  SRR19973071.1715627/1‑150 (MQ=60)
                                                                                                 ATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACC                                                  >  SRR19973071.982887/1‑150 (MQ=60)
                                                                                                 ATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACC                                                  <  SRR19973071.1116873/150‑1 (MQ=60)
                                                                                                      GGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGG                                             >  SRR19973071.1888686/1‑150 (MQ=60)
                                                                                                      GGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGG                                             >  SRR19973071.628120/1‑150 (MQ=60)
                                                                                                      GGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGG                                             >  SRR19973071.628017/1‑150 (MQ=60)
                                                                                                         GGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAG                                          <  SRR19973071.2758367/150‑1 (MQ=60)
                                                                                                           CAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTACACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGAT                                        >  SRR19973071.4088563/1‑150 (MQ=60)
                                                                                                           CAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGAT                                        >  SRR19973071.3270046/1‑150 (MQ=60)
                                                                                                            AGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATC                                       >  SRR19973071.4384516/1‑150 (MQ=60)
                                                                                                              CTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCA                                     <  SRR19973071.1268986/150‑1 (MQ=60)
                                                                                                              CTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCA                                     <  SRR19973071.2425333/150‑1 (MQ=60)
                                                                                                              CTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCA                                     <  SRR19973071.1481790/150‑1 (MQ=60)
                                                                                                              CTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCA                                     <  SRR19973071.1268976/150‑1 (MQ=60)
                                                                                                              CTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCA                                     >  SRR19973071.3011721/1‑150 (MQ=60)
                                                                                                               TCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAG                                    >  SRR19973071.1428335/1‑150 (MQ=60)
                                                                                                               TCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAG                                    >  SRR19973071.1428197/1‑150 (MQ=60)
                                                                                                                CACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTGTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGA                                   >  SRR19973071.4578139/1‑150 (MQ=60)
                                                                                                                CACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGA                                   >  SRR19973071.2936072/1‑150 (MQ=60)
                                                                                                                 ACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGAC                                  <  SRR19973071.4257133/150‑1 (MQ=60)
                                                                                                                 ACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGAC                                  >  SRR19973071.3088164/1‑150 (MQ=60)
                                                                                                                   TGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCA                                >  SRR19973071.2720293/1‑150 (MQ=60)
                                                                                                                      CCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATG                             <  SRR19973071.1233263/150‑1 (MQ=60)
                                                                                                                        GCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGAT                           >  SRR19973071.1656384/1‑150 (MQ=60)
                                                                                                                        GCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGAT                           >  SRR19973071.1656220/1‑150 (MQ=60)
                                                                                                                        GCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGAT                           >  SRR19973071.1656195/1‑150 (MQ=60)
                                                                                                                             ACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCT                      <  SRR19973071.4553033/150‑1 (MQ=60)
                                                                                                                             ACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCT                      <  SRR19973071.1642041/150‑1 (MQ=60)
                                                                                                                             ACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCT                      <  SRR19973071.2556202/150‑1 (MQ=60)
                                                                                                                                CGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATTGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAA                   >  SRR19973071.838238/1‑150 (MQ=60)
                                                                                                                                CGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAA                   >  SRR19973071.1772472/1‑150 (MQ=60)
                                                                                                                                CGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAA                   >  SRR19973071.4601996/1‑150 (MQ=60)
                                                                                                                                 GTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAA                  >  SRR19973071.4012101/1‑150 (MQ=60)
                                                                                                                                 GTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAA                  >  SRR19973071.4012088/1‑150 (MQ=60)
                                                                                                                                   CGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAC                <  SRR19973071.4409454/150‑1 (MQ=60)
                                                                                                                                   CGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAC                <  SRR19973071.3201061/150‑1 (MQ=60)
                                                                                                                                      GCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGC             <  SRR19973071.4757406/150‑1 (MQ=60)
                                                                                                                                       CTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCATTGCCAACGTGCGTAAACCGCTGGGCGCGGTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCC            <  SRR19973071.2061173/150‑1 (MQ=60)
                                                                                                                                          AATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCT         >  SRR19973071.832052/1‑150 (MQ=60)
                                                                                                                                               GCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATA    <  SRR19973071.3855838/150‑1 (MQ=60)
                                                                                                                                                CAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATAT   >  SRR19973071.1075440/1‑150 (MQ=60)
                                                                                                                                                CAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATAT   >  SRR19973071.1724327/1‑150 (MQ=60)
                                                                                                                                                 AGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAATTTGGCACTCACCGCTGGCAGATCCATACCAATGATGAGCTAAAAACCGCCGCTGAATATC  <  SRR19973071.3816875/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CTGACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATC  >  CP009273/2152296‑2152590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑