Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 500,178 G→T 45.3% intergenic (‑26/‑192) fsr ← / → ushA putative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273500,1780GT45.3% 188.2 / 297.2 231intergenic (‑26/‑192)fsr/ushAputative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base G (58/68);  new base T (62/43);  total (120/111)
Fisher's exact test for biased strand distribution p-value = 6.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

ATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTC  >  CP009273/500029‑500326
                                                                                                                                                     |                                                                                                                                                    
aTTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCg                                                                                                                                                      <  1:1370937/150‑1 (MQ=255)
   ggATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATg                                                                                                                                                   <  2:3532207/150‑1 (MQ=255)
    gATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGt                                                                                                                                                  <  1:523699/150‑1 (MQ=255)
     aTCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTa                                                                                                                                                 >  2:3007129/1‑150 (MQ=255)
     aTCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTa                                                                                                                                                 >  2:1054137/1‑150 (MQ=255)
     aTCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTa                                                                                                                                                 <  2:4599739/150‑1 (MQ=255)
     aTCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTa                                                                                                                                                 <  1:714765/150‑1 (MQ=255)
          gTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGaa                                                                                                                                            >  1:3968459/1‑150 (MQ=255)
            cGTTCAGCAGATGTGAGAGGCTGATAGCACCTAACATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATg                                                                                                                                          <  2:2347731/150‑1 (MQ=255)
                 agcagATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGTCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCtt                                                                                                                                     >  2:4088976/1‑150 (MQ=255)
                 agcagATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCtt                                                                                                                                     >  2:2546719/1‑150 (MQ=255)
                     gATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAttt                                                                                                                                 <  2:4511678/150‑1 (MQ=255)
                      aTGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAACGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtg                                                                                                                                <  1:3331028/150‑1 (MQ=255)
                         tgAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGa                                                                                                                             <  1:1092598/150‑1 (MQ=255)
                          gagaGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGat                                                                                                                            >  2:531121/1‑150 (MQ=255)
                           agagGTTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatt                                                                                                                           >  1:3060062/1‑150 (MQ=255)
                           agagGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGatt                                                                                                                           <  1:1439042/150‑1 (MQ=255)
                            gagGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta                                                                                                                          <  2:1166433/150‑1 (MQ=255)
                            gagGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta                                                                                                                          <  1:3987298/150‑1 (MQ=255)
                            gagGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta                                                                                                                          <  1:1031181/150‑1 (MQ=255)
                             agGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGattat                                                                                                                         <  2:42895/150‑1 (MQ=255)
                              ggCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGattatt                                                                                                                        >  2:2069253/1‑150 (MQ=255)
                                       cACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTa                                                                                                               <  2:3212312/150‑1 (MQ=255)
                                       cACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTa                                                                                                               >  1:198041/1‑150 (MQ=255)
                                        aCCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGTTCTTATTTGTGATTATTACCAGACTaa                                                                                                              <  2:908353/150‑1 (MQ=255)
                                        aCCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTaa                                                                                                              >  2:4440548/1‑150 (MQ=255)
                                          cTAAAATACCAAACGATCTTCGGGACTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTCTGGTTGTTCACTCAATGCAAAAGGAAAGTCACATATTCAGGGTTGCGATGAAAATAATGATCTTATTTGTGATTAATACCAGACTATCa                                                                                                            >  2:3107395/1‑150 (MQ=255)
                                            aaaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATa                                                                                                          <  2:1921460/150‑1 (MQ=255)
                                              aaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATAcc                                                                                                        >  1:2411149/1‑150 (MQ=255)
                                              aaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATAcc                                                                                                        <  2:3759488/150‑1 (MQ=255)
                                              aaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATAcc                                                                                                        <  1:2351771/150‑1 (MQ=255)
                                                    aaaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATg                                                                                                  >  2:513918/1‑150 (MQ=255)
                                                     aaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGc                                                                                                 >  2:240212/1‑150 (MQ=255)
                                                     aaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGc                                                                                                 >  2:217692/1‑150 (MQ=255)
                                                       cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgt                                                                                               >  1:1004193/1‑150 (MQ=255)
                                                       cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgt                                                                                               <  2:2598695/150‑1 (MQ=255)
                                                        gATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtc                                                                                              >  2:1264056/1‑150 (MQ=255)
                                                        gATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtc                                                                                              <  1:4408202/150‑1 (MQ=255)
                                                              cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTga                                                                                        <  2:2737392/150‑1 (MQ=255)
                                                               gggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaa                                                                                       >  2:682518/1‑150 (MQ=255)
                                                                ggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaag                                                                                      >  2:3415164/1‑150 (MQ=255)
                                                                   cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaaggaa                                                                                   >  1:1276160/1‑150 (MQ=255)
                                                                       gTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGtct                                                                               <  1:2735882/150‑1 (MQ=255)
                                                                           ttGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAAc                                                                           <  2:4631836/150‑1 (MQ=255)
                                                                            tGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACg                                                                          <  1:1950329/150‑1 (MQ=255)
                                                                               cGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCg                                                                       <  2:2764100/150‑1 (MQ=255)
                                                                                 cAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGaa                                                                     >  1:3291820/1‑150 (MQ=255)
                                                                                  aGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAAt                                                                    >  2:1279811/1‑150 (MQ=255)
                                                                                  aGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAAt                                                                    <  2:3129804/150‑1 (MQ=255)
                                                                                  aGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGACGAAt                                                                    <  1:3157863/150‑1 (MQ=255)
                                                                                    ccGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATAc                                                                  >  1:4531111/1‑150 (MQ=255)
                                                                                     cgcgCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACa                                                                 <  1:745365/150‑1 (MQ=255)
                                                                                      gcgcCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAg                                                                <  2:2207212/150‑1 (MQ=255)
                                                                                       cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGa                                                               <  2:2893392/150‑1 (MQ=255)
                                                                                         cccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACACACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAAt                                                             >  1:128362/1‑150 (MQ=255)
                                                                                         cccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAAt                                                             <  2:4480528/150‑1 (MQ=255)
                                                                                           cgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAAttt                                                           >  1:3792542/1‑150 (MQ=255)
                                                                                            gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTc                                                          >  1:1722632/1‑150 (MQ=255)
                                                                                            gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTc                                                          >  1:1722979/1‑150 (MQ=255)
                                                                                             ccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCt                                                         >  1:1982388/1‑150 (MQ=255)
                                                                                               acaGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTaa                                                       >  1:2134122/1‑150 (MQ=255)
                                                                                                        ggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGc                                              <  2:1598184/150‑1 (MQ=255)
                                                                                                        ggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGc                                              >  2:13759/1‑150 (MQ=255)
                                                                                                         gttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCa                                             <  2:3968464/150‑1 (MQ=255)
                                                                                                         gttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCa                                             <  1:2216486/150‑1 (MQ=255)
                                                                                                            gttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGAt                                          >  2:1454584/1‑150 (MQ=255)
                                                                                                                aCTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt                                      >  1:3217749/1‑150 (MQ=255)
                                                                                                                aCTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt                                      >  1:1259027/1‑150 (MQ=255)
                                                                                                                aCTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt                                      >  1:899593/1‑150 (MQ=255)
                                                                                                                 cTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATc                                     >  2:4042026/1‑150 (MQ=255)
                                                                                                                  tCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCt                                    <  1:320654/150‑1 (MQ=255)
                                                                                                                   cATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCtt                                   >  1:2961697/1‑150 (MQ=255)
                                                                                                                   cATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCtt                                   <  2:2563214/150‑1 (MQ=255)
                                                                                                                   cATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCtt                                   <  2:2740658/150‑1 (MQ=255)
                                                                                                                        ccATAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCg                              >  1:4470213/1‑150 (MQ=255)
                                                                                                                        ccATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCg                              <  1:2007605/150‑1 (MQ=255)
                                                                                                                         cATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCgg                             >  1:4230367/1‑150 (MQ=255)
                                                                                                                          aTAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGa                            <  1:2695465/150‑1 (MQ=255)
                                                                                                                           tAGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGAc                           >  2:3901411/1‑150 (MQ=255)
                                                                                                                            aGGAAAGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACg                          <  1:2883516/150‑1 (MQ=255)
                                                                                                                            aGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACg                          >  1:1774989/1‑150 (MQ=255)
                                                                                                                            aGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACg                          <  1:3584610/150‑1 (MQ=255)
                                                                                                                            aGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACg                          >  1:4370691/1‑150 (MQ=255)
                                                                                                                               aaaGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAg                       <  1:3941201/150‑1 (MQ=255)
                                                                                                                               aaaGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAg                       >  2:603327/1‑150 (MQ=255)
                                                                                                                                aaGTCACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGa                      >  1:3466338/1‑150 (MQ=255)
                                                                                                                                   tCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACtt                   <  1:154003/150‑1 (MQ=255)
                                                                                                                                   tCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACtt                   <  1:151606/150‑1 (MQ=255)
                                                                                                                                    cACTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTg                  >  1:672190/1‑150 (MQ=255)
                                                                                                                                     aCTTTTTCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGt                 <  2:1646443/150‑1 (MQ=255)
                                                                                                                                        ttttCAGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTa              >  1:2832099/1‑150 (MQ=255)
                                                                                                                                            cAGGGTTGCTATGTAAAGAATTATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgat          <  1:547876/150‑1 (MQ=255)
                                                                                                                                             aGGGTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgatg         <  2:1572215/150‑1 (MQ=255)
                                                                                                                                              gggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGt        >  2:4364246/1‑150 (MQ=255)
                                                                                                                                              gggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGt        <  2:1808290/150‑1 (MQ=255)
                                                                                                                                              gggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGt        <  1:3560996/150‑1 (MQ=255)
                                                                                                                                               ggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTc       >  1:231363/1‑150 (MQ=255)
                                                                                                                                               ggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTc       <  1:2780043/150‑1 (MQ=255)
                                                                                                                                                gTTGCTATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTcg      >  2:3479314/1‑150 (MQ=255)
                                                                                                                                                    cGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTc  >  1:350815/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTC  >  CP009273/500029‑500326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A