Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,800,416 C→T 14.6% intergenic (‑76/‑211) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,800,4160CT14.6% 802.9 / 99.6 277intergenic (‑76/‑211)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (95/141);  new base T (19/21);  total (115/162)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

CAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTG  >  CP009273/1800268‑1800565
                                                                                                                                                    |                                                                                                                                                     
cAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTcc                                                                                                                                                      <  1:2987353/150‑1 (MQ=255)
cAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTcc                                                                                                                                                      >  2:1571618/1‑150 (MQ=255)
 aGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTtcc                                                                                                                                                     >  2:77585/1‑150 (MQ=255)
 aGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTccc                                                                                                                                                     >  1:2718223/1‑150 (MQ=255)
 aGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTccc                                                                                                                                                     <  1:2117748/150‑1 (MQ=255)
  gCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCt                                                                                                                                                    <  2:4035466/150‑1 (MQ=255)
  gCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCt                                                                                                                                                    <  2:381001/150‑1 (MQ=255)
   cTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTg                                                                                                                                                   <  2:3698583/150‑1 (MQ=255)
   cTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTg                                                                                                                                                   <  2:4600937/150‑1 (MQ=255)
       aTTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGaaa                                                                                                                                               <  1:3727210/150‑1 (MQ=255)
          cAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGt                                                                                                                                            <  1:2952485/150‑1 (MQ=255)
             tGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAAc                                                                                                                                         <  1:10670/150‑1 (MQ=255)
                    aaCATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATtat                                                                                                                                  >  2:3046030/1‑150 (MQ=255)
                      cATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATaa                                                                                                                                <  1:2219437/150‑1 (MQ=255)
                      cATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATaa                                                                                                                                <  1:2686925/150‑1 (MQ=255)
                      actGCCCCCCGCCATCATATCGATGGCAGGAAGTGTCTTCAAAAGCTTCATTTATCCAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGTTTAGCGTCCCTGGAAAGGTAACGAATTATaa                                                                                                                                <  1:4546948/148‑1 (MQ=255)
                         gCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATaaaaa                                                                                                                             >  1:878687/1‑150 (MQ=255)
                           cccccGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAgg                                                                                                                           >  2:116197/1‑150 (MQ=255)
                            ccccGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGgc                                                                                                                          <  2:2263949/150‑1 (MQ=255)
                            ccccGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGgc                                                                                                                          <  2:12460/150‑1 (MQ=255)
                               cGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGcgcg                                                                                                                       >  2:201609/1‑150 (MQ=255)
                                  cAACATAACGATGGCAGGAACAGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAAt                                                                                                                    >  1:737740/1‑150 (MQ=255)
                                   aaCATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATa                                                                                                                   <  1:1626819/150‑1 (MQ=255)
                                   aaCATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATa                                                                                                                   <  2:4447665/150‑1 (MQ=255)
                                   aaCATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTAAAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAATATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATa                                                                                                                   >  2:3530405/1‑150 (MQ=255)
                                    aCATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATaa                                                                                                                  <  1:1783718/150‑1 (MQ=255)
                                    aCATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATaa                                                                                                                  >  1:958258/1‑150 (MQ=255)
                                         aCGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                                                                             >  2:1499391/1‑150 (MQ=255)
                                         aCGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                                                                             <  2:3953566/150‑1 (MQ=255)
                                         aCGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                                                                             <  1:137725/150‑1 (MQ=255)
                                             tGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAAt                                                                                                         <  1:1898342/150‑1 (MQ=255)
                                               gCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGt                                                                                                       <  2:2479534/150‑1 (MQ=255)
                                               gCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGt                                                                                                       <  2:167145/150‑1 (MQ=255)
                                                 aGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTAt                                                                                                     <  2:4346530/150‑1 (MQ=255)
                                                    aaCTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCt                                                                                                  <  2:4385003/150‑1 (MQ=255)
                                                    aaCTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCt                                                                                                  <  1:4366495/150‑1 (MQ=255)
                                                     aCTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCtt                                                                                                 >  1:2944915/1‑150 (MQ=255)
                                                      cTGTCTTCAAAAGCTTCATTTCTCAAGAGTCCGTACAACAAAAAAAGAGACCAGCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                                                                                <  2:3188577/150‑1 (MQ=255)
                                                      cTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                                                                                >  2:1902431/1‑150 (MQ=255)
                                                      cTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                                                                                >  1:3496041/1‑150 (MQ=255)
                                                             cAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAtt                                                                                         <  2:337977/150‑1 (MQ=255)
                                                               aaaGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAtttt                                                                                       <  1:1998253/150‑1 (MQ=255)
                                                                aaGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt                                                                                      >  2:2127082/1‑150 (MQ=255)
                                                                 aGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAtttttt                                                                                     <  1:3663780/150‑1 (MQ=255)
                                                                 aGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAtttttt                                                                                     <  2:4136984/150‑1 (MQ=255)
                                                                   cTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGa                                                                                   <  2:1410216/150‑1 (MQ=255)
                                                                    ttCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGaa                                                                                  >  1:685474/1‑150 (MQ=255)
                                                                     tCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAAt                                                                                 <  1:4523388/150‑1 (MQ=255)
                                                                      cATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCTCTGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTTGAAAGGTAACGAATTATTAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATa                                                                                <  1:409163/150‑1 (MQ=255)
                                                                       aTTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATaa                                                                               >  1:3158291/1‑150 (MQ=255)
                                                                        tttATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAg                                                                              <  1:2259896/150‑1 (MQ=255)
                                                                          tATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCa                                                                            >  1:1179096/1‑150 (MQ=255)
                                                                          tATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCa                                                                            <  1:4038291/150‑1 (MQ=255)
                                                                            tCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtg                                                                          <  1:3041717/150‑1 (MQ=255)
                                                                             cAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgt                                                                         <  1:1475831/150‑1 (MQ=255)
                                                                              aaGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgtg                                                                        >  2:1648213/1‑150 (MQ=255)
                                                                              aaGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgtg                                                                        <  1:252509/150‑1 (MQ=255)
                                                                               agagTCCGTACAACAAAAAAAGAGACCCTCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTgg                                                                       <  1:2320361/150‑1 (MQ=255)
                                                                                gagTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGc                                                                      <  2:1900021/150‑1 (MQ=255)
                                                                                 agTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCg                                                                     <  1:3224124/150‑1 (MQ=255)
                                                                                      gTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAAc                                                                <  1:4264517/150‑1 (MQ=255)
                                                                                           acaaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtt                                                           >  2:2841932/1‑150 (MQ=255)
                                                                                             aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtttt                                                         <  1:4163447/150‑1 (MQ=255)
                                                                                             aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtttt                                                         <  2:1946634/150‑1 (MQ=255)
                                                                                             aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtttt                                                         >  2:422732/1‑150 (MQ=255)
                                                                                              aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAttttt                                                        >  1:2480930/1‑150 (MQ=255)
                                                                                              aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAttttt                                                        <  2:590937/150‑1 (MQ=255)
                                                                                               aaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTttta                                                       <  1:578273/150‑1 (MQ=255)
                                                                                                  aaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTtttattt                                                    <  1:4426928/150‑1 (MQ=255)
                                                                                                    gagaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTttatttat                                                  <  2:1702434/150‑1 (MQ=255)
                                                                                                     agaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTtata                                                 >  1:909593/1‑150 (MQ=255)
                                                                                                        ccATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTtatatat                                              >  2:3086752/1‑150 (MQ=255)
                                                                                                          aTCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATTTGGCGAAAACAGATTTTTATTTatatatat                                            >  2:1725576/1‑150 (MQ=255)
                                                                                                            cgcgGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATAttt                                          >  2:2294503/1‑150 (MQ=255)
                                                                                                            cgcgGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATAttt                                          <  1:3813565/150‑1 (MQ=255)
                                                                                                              cgGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTAt                                        >  2:3324347/1‑150 (MQ=255)
                                                                                                               ggTCCCTGAAACTTTATTAAGTATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATTTATTCTTATTTCATTTTTTGAATAAGCATGTGTCGAAAACAGATTTTTATTTATATATATTTANc                                       <  2:840296/150‑1 (MQ=255)
                                                                                                               ggTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATc                                       <  2:3261286/150‑1 (MQ=255)
                                                                                                                gTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCt                                      <  1:2409110/150‑1 (MQ=255)
                                                                                                                     ggAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCaaa                                 >  1:3594715/1‑150 (MQ=255)
                                                                                                                     ggAAACTTTCTTAAGGATCAAAGATTAGCGACCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTTCaaa                                 >  2:4542482/1‑150 (MQ=255)
                                                                                                                      gAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCaaaa                                <  1:3066785/150‑1 (MQ=255)
                                                                                                                         aCTTTCTTAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAAttt                             >  2:1447424/1‑150 (MQ=255)
                                                                                                                          cTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAAtttt                            >  1:2145528/1‑150 (MQ=255)
                                                                                                                            ttCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaa                          >  1:243099/1‑150 (MQ=255)
                                                                                                                              cTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaaat                        <  1:2039659/150‑1 (MQ=255)
                                                                                                                               ttAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaaata                       <  2:1313721/150‑1 (MQ=255)
                                                                                                                               ttAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaaata                       <  2:1536113/150‑1 (MQ=255)
                                                                                                                                tAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaaataa                      >  2:3883933/1‑150 (MQ=255)
                                                                                                                                tAAGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTtaaataa                      >  2:2639351/1‑150 (MQ=255)
                                                                                                                                  aGGATCAAAGATTAGCGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAg                    >  2:3622290/1‑150 (MQ=255)
                                                                                                                                  aGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAg                    <  2:2720904/150‑1 (MQ=255)
                                                                                                                                  aGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAg                    <  2:2530635/150‑1 (MQ=255)
                                                                                                                                        aaaGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCaa              >  2:2944658/1‑150 (MQ=255)
                                                                                                                                          aGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATa            >  1:2627630/1‑150 (MQ=255)
                                                                                                                                                 cGTTCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTAGTTATATATATTTATCTGCAAAATTTTAAATAAATCTCCAATAAATCata     >  1:3307680/1‑150 (MQ=255)
                                                                                                                                                 cGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCata     <  2:3764196/150‑1 (MQ=255)
                                                                                                                                                  gTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCatat    <  1:3418685/150‑1 (MQ=255)
                                                                                                                                                    cccTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTg  <  2:569584/150‑1 (MQ=255)
                                                                                                                                                    cccTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTg  <  1:1234563/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTG  >  CP009273/1800268‑1800565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A