Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,874,423 C→A 52.9% L198F (TTG→TTT)  leuE ← leucine efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,874,4230CA52.9% 47.5 / inf 236L198F (TTG→TTT) leuEleucine efflux protein
Reads supporting (aligned to +/- strand):  ref base C (60/51);  new base A (65/60);  total (125/111)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 239 total aligned reads displayed.

TTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874275‑1874572
                                                                                                                                                    |                                                                                                                                                     
 tGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATaaa                                                                                                                                                     <  2:2119864/150‑1 (MQ=255)
  gCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGACACCCTCGAACATCaaa                                                                                                                                                    >  2:2421608/1‑150 (MQ=255)
     ttttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCa                                                                                                                                                 <  1:104924/150‑1 (MQ=255)
      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCaa                                                                                                                                                <  2:2521269/150‑1 (MQ=255)
      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCaa                                                                                                                                                >  1:553997/1‑150 (MQ=255)
       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAt                                                                                                                                               >  1:1434552/1‑150 (MQ=255)
       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAt                                                                                                                                               <  2:3066688/150‑1 (MQ=255)
        tAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATc                                                                                                                                              >  2:3789914/1‑150 (MQ=255)
          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAg                                                                                                                                            <  2:4299212/150‑1 (MQ=255)
          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAg                                                                                                                                            >  2:3206641/1‑150 (MQ=255)
          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAg                                                                                                                                            >  1:2472074/1‑150 (MQ=255)
          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGAGAAACCCACGAACATCAAACCAATCAg                                                                                                                                            <  2:4299196/150‑1 (MQ=255)
             aCCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGa                                                                                                                                         >  2:1274054/1‑150 (MQ=255)
              ccATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAg                                                                                                                                        >  1:3262034/1‑150 (MQ=255)
                aTTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGtt                                                                                                                                      >  2:3874264/1‑150 (MQ=255)
                aTTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGtt                                                                                                                                      <  1:3543391/150‑1 (MQ=255)
                   tATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGcc                                                                                                                                   <  2:2935764/150‑1 (MQ=255)
                      tGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAAc                                                                                                                                >  2:958294/1‑150 (MQ=255)
                        gTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTACAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACtt                                                                                                                              >  2:23536/1‑150 (MQ=255)
                         ttACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACttt                                                                                                                             >  1:3830961/1‑150 (MQ=255)
                          tACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTa                                                                                                                            <  1:4123917/150‑1 (MQ=255)
                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAg                                                                                                                           >  2:4314303/1‑150 (MQ=255)
                            cTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGc                                                                                                                          <  1:2392609/150‑1 (MQ=255)
                            cTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGc                                                                                                                          >  2:383271/1‑150 (MQ=255)
                             tttttAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGcc                                                                                                                         <  1:3136468/150‑1 (MQ=255)
                             tttttAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGcc                                                                                                                         >  1:1300355/1‑150 (MQ=255)
                                      ccATATCAGCGAAGAATCAGGGAGGATTACAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCttt                                                                                                                <  2:1248462/150‑1 (MQ=255)
                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTg                                                                                                             <  2:2104777/150‑1 (MQ=255)
                                            cAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTa                                                                                                          <  1:4318563/150‑1 (MQ=255)
                                              gCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACg                                                                                                        >  1:3346756/1‑150 (MQ=255)
                                                gaagaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTa                                                                                                      <  1:4027092/150‑1 (MQ=255)
                                                   gaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGt                                                                                                   >  1:3234841/1‑150 (MQ=255)
                                                   gaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGt                                                                                                   >  1:4539541/1‑150 (MQ=255)
                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt                                                                                                <  1:641461/150‑1 (MQ=255)
                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt                                                                                                >  1:616639/1‑150 (MQ=255)
                                                         gggAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCg                                                                                             >  2:1362313/1‑150 (MQ=255)
                                                          ggaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGt                                                                                            >  1:1541316/1‑150 (MQ=255)
                                                           gaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg                                                                                           >  1:2813087/1‑150 (MQ=255)
                                                           gaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg                                                                                           >  2:4132951/1‑150 (MQ=255)
                                                            aggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGa                                                                                          <  1:3031592/150‑1 (MQ=255)
                                                              gaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa                                                                                        >  1:896116/1‑150 (MQ=255)
                                                               aTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaa                                                                                       <  2:288497/150‑1 (MQ=255)
                                                                ttATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaa                                                                                      <  2:590691/150‑1 (MQ=255)
                                                                 tataGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaaa                                                                                     >  2:3087496/1‑150 (MQ=255)
                                                                  ataGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAg                                                                                    >  2:4004676/1‑150 (MQ=255)
                                                                   taGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGc                                                                                   >  1:4440045/1‑150 (MQ=255)
                                                                    aGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa                                                                                  >  1:514295/1‑150 (MQ=255)
                                                                    aGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa                                                                                  <  1:2247587/150‑1 (MQ=255)
                                                                       tGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCa                                                                               >  1:2086556/1‑150 (MQ=255)
                                                                       tGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCa                                                                               <  1:1917217/150‑1 (MQ=255)
                                                                        gggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAg                                                                              <  2:4576121/150‑1 (MQ=255)
                                                                         ggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGa                                                                             >  2:1695109/1‑150 (MQ=255)
                                                                          gAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGat                                                                            <  2:3384451/150‑1 (MQ=255)
                                                                           aaGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGata                                                                           >  1:1761900/1‑150 (MQ=255)
                                                                           aaGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGata                                                                           <  2:2832046/150‑1 (MQ=255)
                                                                              cccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTNTGTACTGCGTGACAAAAGCACCAGATATaa                                                                        <  1:4281025/150‑1 (MQ=255)
                                                                                cgcgACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATc                                                                      <  2:548124/150‑1 (MQ=255)
                                                                                  cgACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAg                                                                    <  1:285636/150‑1 (MQ=255)
                                                                                   gACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAgg                                                                   >  2:3679239/1‑150 (MQ=255)
                                                                                    aCAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGa                                                                  >  1:1515722/1‑150 (MQ=255)
                                                                                      aaCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAg                                                                >  1:2574162/1‑150 (MQ=255)
                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               >  1:1038881/1‑150 (MQ=255)
                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               >  1:2599591/1‑150 (MQ=255)
                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCACTCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               <  2:2485145/150‑1 (MQ=255)
                                                                                        cgcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATACTCAGGAAGCt                                                              >  2:201267/1‑150 (MQ=255)
                                                                                         gcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTc                                                             <  1:1870889/150‑1 (MQ=255)
                                                                                           gggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaa                                                           >  2:555187/1‑150 (MQ=255)
                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          >  2:834535/1‑150 (MQ=255)
                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          >  2:3283207/1‑150 (MQ=255)
                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          <  1:3789914/150‑1 (MQ=255)
                                                                                              cTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATa                                                        >  1:1449109/1‑150 (MQ=255)
                                                                                               tGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAg                                                       >  1:1015186/1‑150 (MQ=255)
                                                                                               tGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAg                                                       >  2:4464459/1‑150 (MQ=255)
                                                                                                   aGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGa                                                   <  2:344069/150‑1 (MQ=255)
                                                                                                     cATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGaaa                                                 <  2:2541019/150‑1 (MQ=255)
                                                                                                      aTCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAAc                                                >  2:4396568/1‑150 (MQ=255)
                                                                                                       tCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACt                                               >  1:3931532/1‑150 (MQ=255)
                                                                                                         aGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCa                                             >  2:746335/1‑150 (MQ=255)
                                                                                                          ggATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAc                                            >  1:3833712/1‑150 (MQ=255)
                                                                                                           gATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAcc                                           <  2:805105/150‑1 (MQ=255)
                                                                                                           gATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAcc                                           <  2:4369880/150‑1 (MQ=255)
                                                                                                               gCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGtt                                       >  1:1390885/1‑150 (MQ=255)
                                                                                                                 aGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTcc                                     >  2:1452154/1‑150 (MQ=255)
                                                                                                                     tCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCg                                 >  1:758770/1‑150 (MQ=255)
                                                                                                                        ccAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcg                              >  1:1430133/1‑150 (MQ=255)
                                                                                                                         cAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgc                             >  1:1942104/1‑150 (MQ=255)
                                                                                                                          aGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgcc                            <  1:2766216/150‑1 (MQ=255)
                                                                                                                               ggCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAg                       <  1:1396226/150‑1 (MQ=255)
                                                                                                                                   gCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATa                   <  1:4375241/150‑1 (MQ=255)
                                                                                                                                    cGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaa                  <  1:3958538/150‑1 (MQ=255)
                                                                                                                                     gAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaaa                 <  2:1139040/150‑1 (MQ=255)
                                                                                                                                     gAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaaa                 <  2:3320530/150‑1 (MQ=255)
                                                                                                                                      aaaCCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAg                <  2:4540461/150‑1 (MQ=255)
                                                                                                                                       aaCCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGa               >  1:411374/1‑150 (MQ=255)
                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:2599591/150‑1 (MQ=255)
                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:2298992/150‑1 (MQ=255)
                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:1038990/150‑1 (MQ=255)
                                                                                                                                         cccACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAAt             <  1:2429918/150‑1 (MQ=255)
                                                                                                                                          ccACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATg            >  2:1399182/1‑150 (MQ=255)
                                                                                                                                            aCGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGaa          <  2:179606/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874275‑1874572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874274‑1874572
                                                                                                                                                     |                                                                                                                                                     
TTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATA                                                                                                                                                       <  SRR19973073.92654/150‑1 (MQ=60)
TTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATA                                                                                                                                                       <  SRR19973073.92651/150‑1 (MQ=60)
 TTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCA                                                                                                                                                      >  SRR19973073.4230359/1‑150 (MQ=60)
   GCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAA                                                                                                                                                    >  SRR19973073.3986572/1‑150 (MQ=60)
     ATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACC                                                                                                                                                  <  SRR19973073.2597296/150‑1 (MQ=60)
      TTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCA                                                                                                                                                 <  SRR19973073.2014333/150‑1 (MQ=60)
      TTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCA                                                                                                                                                 <  SRR19973073.105183/150‑1 (MQ=60)
       TTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAA                                                                                                                                                >  SRR19973073.555129/1‑150 (MQ=60)
       TTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAA                                                                                                                                                >  SRR19973073.555193/1‑150 (MQ=60)
        TTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAT                                                                                                                                               <  SRR19973073.3848539/150‑1 (MQ=60)
        TTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAT                                                                                                                                               <  SRR19973073.335322/150‑1 (MQ=60)
        TTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAT                                                                                                                                               >  SRR19973073.1437572/1‑150 (MQ=60)
           AATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAG                                                                                                                                            >  SRR19973073.2477191/1‑150 (MQ=60)
           AATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAG                                                                                                                                            >  SRR19973073.2477162/1‑150 (MQ=60)
               CCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAG                                                                                                                                        >  SRR19973073.3268637/1‑150 (MQ=60)
                 ATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTT                                                                                                                                      <  SRR19973073.3550487/150‑1 (MQ=60)
                 ATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTT                                                                                                                                      <  SRR19973073.258612/150‑1 (MQ=60)
                    TATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCC                                                                                                                                   >  SRR19973073.839106/1‑150 (MQ=60)
                     ATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCA                                                                                                                                  <  SRR19973073.1518589/150‑1 (MQ=60)
                     ATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCA                                                                                                                                  <  SRR19973073.2858415/150‑1 (MQ=60)
                     ATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCA                                                                                                                                  <  SRR19973073.2858529/150‑1 (MQ=60)
                       TGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAAC                                                                                                                                <  SRR19973073.2753662/150‑1 (MQ=60)
                       TGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAAC                                                                                                                                <  SRR19973073.666344/150‑1 (MQ=60)
                       TGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAAC                                                                                                                                <  SRR19973073.2303573/150‑1 (MQ=60)
                          TTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTT                                                                                                                             >  SRR19973073.3838578/1‑150 (MQ=60)
                           TACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTA                                                                                                                            <  SRR19973073.4132167/150‑1 (MQ=60)
                           TACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTA                                                                                                                            <  SRR19973073.3817931/150‑1 (MQ=60)
                            ACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAG                                                                                                                           <  SRR19973073.63439/150‑1 (MQ=60)
                            ACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAG                                                                                                                           <  SRR19973073.63572/150‑1 (MQ=60)
                            ACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAG                                                                                                                           <  SRR19973073.3327896/150‑1 (MQ=60)
                             CTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGC                                                                                                                          <  SRR19973073.2397531/150‑1 (MQ=60)
                             CTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGC                                                                                                                          <  SRR19973073.2397523/150‑1 (MQ=60)
                             CTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGC                                                                                                                          >  SRR19973073.1401277/1‑150 (MQ=60)
                             CTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGC                                                                                                                          <  SRR19973073.2397518/150‑1 (MQ=60)
                              TTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCC                                                                                                                         <  SRR19973073.3142777/150‑1 (MQ=60)
                              TTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCC                                                                                                                         >  SRR19973073.1303098/1‑150 (MQ=60)
                                TTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAG                                                                                                                       <  SRR19973073.1435923/150‑1 (MQ=60)
                                    GCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTC                                                                                                                   >  SRR19973073.3535367/1‑150 (MQ=60)
                                         ATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTT                                                                                                              >  SRR19973073.3688499/1‑150 (MQ=60)
                                         ATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTT                                                                                                              >  SRR19973073.3839841/1‑150 (MQ=60)
                                          TATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTG                                                                                                             >  SRR19973073.3156148/1‑150 (MQ=60)
                                          TATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTG                                                                                                             >  SRR19973073.4643363/1‑150 (MQ=60)
                                          TATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTG                                                                                                             <  SRR19973073.1821936/150‑1 (MQ=60)
                                           ATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGG                                                                                                            >  SRR19973073.1448400/1‑150 (MQ=60)
                                             CAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTA                                                                                                          <  SRR19973073.4327192/150‑1 (MQ=60)
                                             CAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTA                                                                                                          >  SRR19973073.3715662/1‑150 (MQ=60)
                                             CAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTA                                                                                                          >  SRR19973073.583835/1‑150 (MQ=60)
                                               GCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACG                                                                                                        >  SRR19973073.3353518/1‑150 (MQ=60)
                                                 GAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTA                                                                                                      >  SRR19973073.2561109/1‑150 (MQ=60)
                                                 GAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTA                                                                                                      <  SRR19973073.4035148/150‑1 (MQ=60)
                                                 GAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTA                                                                                                      <  SRR19973073.817531/150‑1 (MQ=60)
                                                 GAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTA                                                                                                      >  SRR19973073.3966833/1‑150 (MQ=60)
                                                  AAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTAT                                                                                                     <  SRR19973073.1635150/150‑1 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   >  SRR19973073.4311630/1‑150 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   >  SRR19973073.4548642/1‑150 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   <  SRR19973073.3925398/150‑1 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   >  SRR19973073.3241367/1‑150 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   >  SRR19973073.4548695/1‑150 (MQ=60)
                                                    GAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGT                                                                                                   >  SRR19973073.1376902/1‑150 (MQ=60)
                                                       TCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACT                                                                                                <  SRR19973073.642822/150‑1 (MQ=60)
                                                       TCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACT                                                                                                <  SRR19973073.3140365/150‑1 (MQ=60)
                                                       TCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACT                                                                                                >  SRR19973073.617957/1‑150 (MQ=60)
                                                           GGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGT                                                                                            >  SRR19973073.1544466/1‑150 (MQ=60)
                                                            GAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTG                                                                                           >  SRR19973073.2818852/1‑150 (MQ=60)
                                                             AGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGA                                                                                          <  SRR19973073.3037719/150‑1 (MQ=60)
                                                             AGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGA                                                                                          <  SRR19973073.4126815/150‑1 (MQ=60)
                                                               GATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACA                                                                                        >  SRR19973073.897965/1‑150 (MQ=60)
                                                                   ATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAG                                                                                    >  SRR19973073.4410600/1‑150 (MQ=60)
                                                                    TAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGC                                                                                   >  SRR19973073.4448961/1‑150 (MQ=60)
                                                                     AGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCA                                                                                  >  SRR19973073.515400/1‑150 (MQ=60)
                                                                     AGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCA                                                                                  <  SRR19973073.2252212/150‑1 (MQ=60)
                                                                       ATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACC                                                                                <  SRR19973073.220682/150‑1 (MQ=60)
                                                                       ATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACC                                                                                <  SRR19973073.221303/150‑1 (MQ=60)
                                                                        TGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCA                                                                               >  SRR19973073.2090921/1‑150 (MQ=60)
                                                                        TGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCA                                                                               <  SRR19973073.1921121/150‑1 (MQ=60)
                                                                            AAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATA                                                                           >  SRR19973073.1765548/1‑150 (MQ=60)
                                                                               CCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTNTGTACTGCGTGACAAAAGCACCAGATATAA                                                                        <  SRR19973073.4289523/150‑1 (MQ=60)
                                                                                   CGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAG                                                                    <  SRR19973073.286254/150‑1 (MQ=60)
                                                                                     ACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGA                                                                  >  SRR19973073.1518846/1‑150 (MQ=60)
                                                                                       AACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAG                                                                >  SRR19973073.2579466/1‑150 (MQ=60)
                                                                                        ACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGC                                                               >  SRR19973073.1041160/1‑150 (MQ=60)
                                                                                        ACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGC                                                               >  SRR19973073.2604941/1‑150 (MQ=60)
                                                                                        ACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGC                                                               >  SRR19973073.1041049/1‑150 (MQ=60)
                                                                                          GCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTC                                                             <  SRR19973073.1874722/150‑1 (MQ=60)
                                                                                           CGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCA                                                            <  SRR19973073.4042132/150‑1 (MQ=60)
                                                                                             GGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAA                                                          <  SRR19973073.3797491/150‑1 (MQ=60)
                                                                                              GCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAAT                                                         >  SRR19973073.3256243/1‑150 (MQ=60)
                                                                                               CTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATA                                                        >  SRR19973073.1452155/1‑150 (MQ=60)
                                                                                               CTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATA                                                        >  SRR19973073.1451491/1‑150 (MQ=60)
                                                                                                TGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAG                                                       >  SRR19973073.1017450/1‑150 (MQ=60)
                                                                                                TGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAG                                                       >  SRR19973073.1017314/1‑150 (MQ=60)
                                                                                                TGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAG                                                       <  SRR19973073.171605/150‑1 (MQ=60)
                                                                                                   AAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAG                                                    >  SRR19973073.1521136/1‑150 (MQ=60)
                                                                                                       ATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAAC                                                <  SRR19973073.306264/150‑1 (MQ=60)
                                                                                                        TCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACT                                               >  SRR19973073.3939439/1‑150 (MQ=60)
                                                                                                        TCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACT                                               >  SRR19973073.3939360/1‑150 (MQ=60)
                                                                                                           GGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAC                                            >  SRR19973073.3841331/1‑150 (MQ=60)
                                                                                                                GCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTT                                       >  SRR19973073.125723/1‑150 (MQ=60)
                                                                                                                GCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTT                                       >  SRR19973073.1393826/1‑150 (MQ=60)
                                                                                                                      TCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCG                                 >  SRR19973073.2940515/1‑150 (MQ=60)
                                                                                                                      TCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCG                                 >  SRR19973073.760298/1‑150 (MQ=60)
                                                                                                                       CGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGT                                <  SRR19973073.4463444/150‑1 (MQ=60)
                                                                                                                         CCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCG                              >  SRR19973073.1433144/1‑150 (MQ=60)
                                                                                                                          CAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGC                             >  SRR19973073.1946078/1‑150 (MQ=60)
                                                                                                                          CAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGC                             >  SRR19973073.1946074/1‑150 (MQ=60)
                                                                                                                           AGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCC                            <  SRR19973073.2771878/150‑1 (MQ=60)
                                                                                                                            GTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCG                           <  SRR19973073.4393304/150‑1 (MQ=60)
                                                                                                                              CGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCC                         >  SRR19973073.2798120/1‑150 (MQ=60)
                                                                                                                              CGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCC                         >  SRR19973073.4279812/1‑150 (MQ=60)
                                                                                                                                GGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAG                       >  SRR19973073.1220849/1‑150 (MQ=60)
                                                                                                                                GGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAG                       <  SRR19973073.1399177/150‑1 (MQ=60)
                                                                                                                                   AGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAAT                    <  SRR19973073.1913330/150‑1 (MQ=60)
                                                                                                                                   AGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAAT                    <  SRR19973073.1913274/150‑1 (MQ=60)
                                                                                                                                    GCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATA                   <  SRR19973073.4384016/150‑1 (MQ=60)
                                                                                                                                     CGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAA                  <  SRR19973073.3966424/150‑1 (MQ=60)
                                                                                                                                       AAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAG                >  SRR19973073.1417058/1‑150 (MQ=60)
                                                                                                                                        AACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGA               >  SRR19973073.412193/1‑150 (MQ=60)
                                                                                                                                          CCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAAT             <  SRR19973073.2434940/150‑1 (MQ=60)
                                                                                                                                               GAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAAT        <  SRR19973073.2621360/150‑1 (MQ=60)
                                                                                                                                               GAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAAT        <  SRR19973073.2621310/150‑1 (MQ=60)
                                                                                                                                                   ATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCC    >  SRR19973073.4350323/1‑150 (MQ=60)
                                                                                                                                                     CAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  SRR19973073.1397318/1‑150 (MQ=60)
                                                                                                                                                     AAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  SRR19973073.522453/1‑150 (MQ=60)
                                                                                                                                                     AAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  SRR19973073.522241/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874274‑1874572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑