Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,155,768 T→C 47.5% I275T (ATT→ACT)  mdtD → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,155,7680TC47.5% 11.3 / inf 276I275T (ATT→ACT) mdtDputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (60/85);  new base C (70/61);  total (130/146)
Fisher's exact test for biased strand distribution p-value = 5.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 282 total aligned reads displayed.

GACGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCGCTTTG  >  CP009273/2155619‑2155917
                                                                                                                                                     |                                                                                                                                                     
 aCGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTAtt                                                                                                                                                      >  2:3266484/1‑150 (MQ=255)
  cGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTg                                                                                                                                                     <  1:2455071/150‑1 (MQ=255)
     ttGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCa                                                                                                                                                  <  2:225146/150‑1 (MQ=255)
     ttGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCa                                                                                                                                                  <  1:3994004/150‑1 (MQ=255)
       gCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGt                                                                                                                                                >  1:3169866/1‑150 (MQ=255)
         aGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTATTCGCTGGGCCTGGCGGGGAGCTTTGCCGCACGTATTGGCAGTgg                                                                                                                                              >  1:794371/1‑150 (MQ=255)
           gCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCa                                                                                                                                            <  1:4158901/150‑1 (MQ=255)
             cTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATg                                                                                                                                          >  1:3419981/1‑150 (MQ=255)
                 tCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGc                                                                                                                                      <  2:3173748/150‑1 (MQ=255)
                     aGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCtt                                                                                                                                  <  1:1570246/150‑1 (MQ=255)
                     aGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCtt                                                                                                                                  <  2:805177/150‑1 (MQ=255)
                       ttGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTa                                                                                                                                <  1:3465556/150‑1 (MQ=255)
                       ttGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTa                                                                                                                                <  2:1048351/150‑1 (MQ=255)
                        tGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTTGCAGTGGCATGTTGCCCTTTAt                                                                                                                               <  1:2696078/150‑1 (MQ=255)
                            gtggtgGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGaca                                                                                                                           <  2:4594010/150‑1 (MQ=255)
                            gtggtgGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGaca                                                                                                                           <  1:926391/150‑1 (MQ=255)
                             tggtggCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGacac                                                                                                                          >  2:2024320/1‑150 (MQ=255)
                                   cACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGACATGTTGCCCTTTATGACACCGGttt                                                                                                                    <  2:4412791/150‑1 (MQ=255)
                                    aCTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGtttt                                                                                                                   <  1:2921381/150‑1 (MQ=255)
                                      tGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTcc                                                                                                                 >  1:1132031/1‑150 (MQ=255)
                                       ggTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCt                                                                                                                <  2:2923790/150‑1 (MQ=255)
                                        gTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTg                                                                                                               <  1:2879191/150‑1 (MQ=255)
                                         tGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGc                                                                                                              <  1:2910530/150‑1 (MQ=255)
                                           cTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCaa                                                                                                            >  2:1780874/1‑150 (MQ=255)
                                             ttATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAAt                                                                                                          >  1:333857/1‑150 (MQ=255)
                                              tATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAAtt                                                                                                         >  2:3034437/1‑150 (MQ=255)
                                               aTCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTg                                                                                                        >  2:148694/1‑150 (MQ=255)
                                               aTCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTg                                                                                                        <  2:3660945/150‑1 (MQ=255)
                                               aTCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTg                                                                                                        <  2:2096062/150‑1 (MQ=255)
                                                   gctgcACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCt                                                                                                    <  2:3971231/150‑1 (MQ=255)
                                                       cACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGt                                                                                                >  2:2650187/1‑150 (MQ=255)
                                                       cACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGt                                                                                                >  2:1910718/1‑150 (MQ=255)
                                                         cGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGttt                                                                                              <  1:470669/150‑1 (MQ=255)
                                                          gCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTtc                                                                                             <  2:2101968/150‑1 (MQ=255)
                                                           ccAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTTGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTtct                                                                                            <  2:1303571/150‑1 (MQ=255)
                                                            cAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTtctc                                                                                           >  2:1181782/1‑150 (MQ=255)
                                                             aGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCg                                                                                          >  1:2130702/1‑150 (MQ=255)
                                                             aGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCg                                                                                          <  1:3392416/150‑1 (MQ=255)
                                                                aaTAACCACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCg                                                                                       <  1:2588/150‑1 (MQ=255)
                                                                      aacCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCAt                                                                                 >  2:600885/1‑150 (MQ=255)
                                                                       acCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATg                                                                                <  2:3608925/150‑1 (MQ=255)
                                                                          gTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCg                                                                             >  2:4475000/1‑150 (MQ=255)
                                                                          gTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCg                                                                             >  2:4440072/1‑150 (MQ=255)
                                                                             cccTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGAc                                                                          >  2:3321592/1‑150 (MQ=255)
                                                                             cccTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGAc                                                                          >  1:1735767/1‑150 (MQ=255)
                                                                              ccTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACt                                                                         <  2:1834828/150‑1 (MQ=255)
                                                                               cTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACtg                                                                        >  1:2311300/1‑150 (MQ=255)
                                                                                tGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACtga                                                                       >  2:2082661/1‑150 (MQ=255)
                                                                                 gTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACtgat                                                                      >  2:3041451/1‑150 (MQ=255)
                                                                                   tCAGTCTGAAACTGTTCCGGACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACtgatga                                                                    <  2:1615014/150‑1 (MQ=255)
                                                                                       tCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTgatgatgat                                                                >  2:3747493/1‑150 (MQ=255)
                                                                                        cTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATc                                                               >  1:1974359/1‑150 (MQ=255)
                                                                                        cTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATc                                                               >  2:2577503/1‑150 (MQ=255)
                                                                                         tGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATcc                                                              <  2:3477191/150‑1 (MQ=255)
                                                                                          gAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATccc                                                             >  2:2374575/1‑150 (MQ=255)
                                                                                           aaaCTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCg                                                            <  2:1693027/150‑1 (MQ=255)
                                                                                             aCTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGAt                                                          <  1:2437812/150‑1 (MQ=255)
                                                                                              cTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATg                                                         >  1:2818950/1‑150 (MQ=255)
                                                                                               tGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATgg                                                        >  2:545630/1‑150 (MQ=255)
                                                                                                   ccGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCt                                                    <  1:3741466/150‑1 (MQ=255)
                                                                                                    cGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCtt                                                   >  2:1239543/1‑150 (MQ=255)
                                                                                                     gTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTg                                                  >  2:2465821/1‑150 (MQ=255)
                                                                                                     gTACTCGTACC‑TTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTgg                                                 >  2:4391758/1‑150 (MQ=255)
                                                                                                      tACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTgg                                                 >  2:1397869/1‑150 (MQ=255)
                                                                                                             aCCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATg                                          <  1:3559883/150‑1 (MQ=255)
                                                                                                              ccTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATgg                                         >  1:1068668/1‑150 (MQ=255)
                                                                                                              ccTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATgg                                         <  2:569193/150‑1 (MQ=255)
                                                                                                              ccTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATgg                                         >  2:1727722/1‑150 (MQ=255)
                                                                                                              ccTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATgg                                         >  2:933780/1‑150 (MQ=255)
                                                                                                                ttttCGCTGGGCCTGGCGGGGAGCTTTGCCTGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGa                                       >  1:1952961/1‑150 (MQ=255)
                                                                                                                 tttCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGaa                                      <  2:4095938/150‑1 (MQ=255)
                                                                                                                   tCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTCCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATg                                    <  1:1189789/150‑1 (MQ=255)
                                                                                                                    cGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGa                                   >  1:2558654/1‑150 (MQ=255)
                                                                                                                    cGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGa                                   >  1:2050295/1‑150 (MQ=255)
                                                                                                                     gCTGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGaa                                  >  1:4332100/1‑150 (MQ=255)
                                                                                                                       tGGGCCTGGCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGc                                >  2:2540570/1‑150 (MQ=255)
                                                                                                                         ggCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGa                              <  1:1712755/150‑1 (MQ=255)
                                                                                                                              ggCGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATtgt                         <  2:1121338/150‑1 (MQ=255)
                                                                                                                                cGGGGAGCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTgg                       <  1:572085/150‑1 (MQ=255)
                                                                                                                                 ggggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGACTTGTGGt                      >  1:4037430/1‑150 (MQ=255)
                                                                                                                                 ggggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGt                      >  2:1312890/1‑150 (MQ=255)
                                                                                                                                 ggggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTATATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGt                      >  2:3224405/1‑150 (MQ=255)
                                                                                                                                   ggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTAc                    <  2:3011627/150‑1 (MQ=255)
                                                                                                                                   ggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTAc                    <  1:1942110/150‑1 (MQ=255)
                                                                                                                                   ggAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTAc                    >  2:1004539/1‑150 (MQ=255)
                                                                                                                                     aGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACGGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAg                  >  1:1881208/1‑150 (MQ=255)
                                                                                                                                      gCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAgg                 <  1:1450405/150‑1 (MQ=255)
                                                                                                                                      gCTTTGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAgg                 >  1:3264650/1‑150 (MQ=255)
                                                                                                                                       cTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAggt                <  1:2905178/150‑1 (MQ=255)
                                                                                                                                        tttGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGGTGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAggtg               >  1:1645883/1‑150 (MQ=255)
                                                                                                                                        tttGCCGGACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAggtg               <  1:2245108/150‑1 (MQ=255)
                                                                                                                                              ggACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAAt         >  1:445961/1‑150 (MQ=255)
                                                                                                                                              ggACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAAt         <  1:3952472/150‑1 (MQ=255)
                                                                                                                                               gACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATc        >  2:4545519/1‑150 (MQ=255)
                                                                                                                                               gACGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTGTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATc        <  2:2759404/150‑1 (MQ=255)
                                                                                                                                                aCGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCg       <  1:1024045/150‑1 (MQ=255)
                                                                                                                                                aCGTACTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCg       <  1:1036830/150‑1 (MQ=255)
                                                                                                                                                 cGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCGc      >  1:2648178/1‑150 (MQ=255)
                                                                                                                                                 cGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCGc      <  1:1818765/150‑1 (MQ=255)
                                                                                                                                                     ttGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCGCTTTg  <  2:120991/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATCTGCTGCACGCCAGAAATAACAACCGTGCCCTGTTCAGTCTGAAACTGTTCCGTACTCGTACCTTTTCGCTGGGCCTGGCGGGGAGCTTTGCCGGACGTATTGGCAGTGGCATGTTGCCCTTTATGACACCGGTTTTCCTGCAAATTGGCCTCGGTTTCTCGCCGTTTCATGCCGGACTGATGATGATCCCGATGGTGCTTGGCAGCATGGGAATGAAGCGAATTGTGGTACAGGTGGTGAATCGCTTTG  >  CP009273/2155619‑2155917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A