Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,376,959 C→T 8.5% N256N (AAC→AAT)  elaD → protease, capable of cleaving an AMC‑ubiquitin model substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,376,9590CT 8.5% 915.5 / 49.3 271N256N (AAC→AAT) elaDprotease, capable of cleaving an AMC‑ubiquitin model substrate
Reads supporting (aligned to +/- strand):  ref base C (137/110);  new base T (17/6);  total (155/116)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 277 total aligned reads displayed.

CTCAATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCA  >  CP009273/2376810‑2377108
                                                                                                                                                     |                                                                                                                                                     
ctcAATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAAc                                                                                                                                                       <  2:1736775/150‑1 (MQ=255)
 tcAATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCAAAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAAAGCGTCATATTCAACt                                                                                                                                                      >  1:1957608/1‑150 (MQ=255)
  cAATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTc                                                                                                                                                     >  1:897539/1‑150 (MQ=255)
   aaTGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCa                                                                                                                                                    <  2:119114/150‑1 (MQ=255)
   aaTGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCa                                                                                                                                                    <  2:2391665/150‑1 (MQ=255)
      gAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTg                                                                                                                                                 <  2:4386079/150‑1 (MQ=255)
         aaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGt                                                                                                                                              >  1:651372/1‑150 (MQ=255)
            atTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCa                                                                                                                                           >  2:3897840/1‑150 (MQ=255)
               aaGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTa                                                                                                                                        >  1:252228/1‑150 (MQ=255)
                       tCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTATGCTAAGa                                                                                                                                >  1:3759353/1‑150 (MQ=255)
                       tCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGa                                                                                                                                <  2:423508/150‑1 (MQ=255)
                         catcatGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATa                                                                                                                              <  1:434220/150‑1 (MQ=255)
                          atcatGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATaa                                                                                                                             >  1:3884170/1‑150 (MQ=255)
                          atcatGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATaa                                                                                                                             >  2:3394431/1‑150 (MQ=255)
                             atGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGa                                                                                                                          <  2:1673549/150‑1 (MQ=255)
                             atGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGa                                                                                                                          >  2:3100309/1‑150 (MQ=255)
                                     aTGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCAc                                                                                                                  <  2:126893/150‑1 (MQ=255)
                                          gTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAg                                                                                                             >  1:4017486/1‑150 (MQ=255)
                                            ccAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCg                                                                                                           >  1:3027338/1‑150 (MQ=255)
                                               gTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCgtgt                                                                                                        <  2:4039420/150‑1 (MQ=255)
                                                   ttCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCAtt                                                                                                    <  1:311331/150‑1 (MQ=255)
                                                    tCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTa                                                                                                   <  2:1953000/150‑1 (MQ=255)
                                                     ccccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTaa                                                                                                  >  1:4202297/1‑150 (MQ=255)
                                                       ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATACACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATa                                                                                                <  2:3572224/150‑1 (MQ=255)
                                                       ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATa                                                                                                <  1:515692/150‑1 (MQ=255)
                                                        cATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAg                                                                                               <  1:610146/150‑1 (MQ=255)
                                                        cATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAg                                                                                               <  1:1360420/150‑1 (MQ=255)
                                                            aaTACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGtttt                                                                                           <  1:922694/150‑1 (MQ=255)
                                                             aTACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTa                                                                                          >  2:4602189/1‑150 (MQ=255)
                                                              tACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTaa                                                                                         >  1:156038/1‑150 (MQ=255)
                                                               aCTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAAt                                                                                        >  1:3316937/1‑150 (MQ=255)
                                                                   gCGGACACTGGATATTAGTTTCGCTACAGAAAATATTATATAAAAAAAATAATAACCTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATTGTTTTAATTCTg                                                                                    <  2:30199/150‑1 (MQ=255)
                                                                   gCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTg                                                                                    <  1:238460/150‑1 (MQ=255)
                                                                     ggACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGaa                                                                                  >  2:680888/1‑150 (MQ=255)
                                                                            ggATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATgg                                                                           <  1:2960487/150‑1 (MQ=255)
                                                                             gATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATggg                                                                          <  2:2757743/150‑1 (MQ=255)
                                                                             gATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATggg                                                                          <  2:698484/150‑1 (MQ=255)
                                                                                atTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGgaga                                                                       <  2:274799/150‑1 (MQ=255)
                                                                                atTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAAAGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGgaga                                                                       <  2:274917/150‑1 (MQ=255)
                                                                                  tAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGaaa                                                                     <  1:2099268/150‑1 (MQ=255)
                                                                                  tAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGaaa                                                                     <  2:1499039/150‑1 (MQ=255)
                                                                                      ttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTc                                                                 >  1:1031559/1‑150 (MQ=255)
                                                                                      ttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTc                                                                 >  2:2076945/1‑150 (MQ=255)
                                                                                      ttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTc                                                                 >  1:3697164/1‑150 (MQ=255)
                                                                                       tCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCg                                                                <  2:695316/150‑1 (MQ=255)
                                                                                       tCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCg                                                                <  2:3823703/150‑1 (MQ=255)
                                                                                           tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaata                                                            <  1:1901082/150‑1 (MQ=255)
                                                                                           tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaata                                                            <  2:2246443/150‑1 (MQ=255)
                                                                                             cAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATTGTTTTAATTCTGAACTCATGGGAGAAATGTCGaataat                                                          >  2:4127909/1‑150 (MQ=255)
                                                                                               gAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaataataa                                                        >  2:3456880/1‑150 (MQ=255)
                                                                                                  aaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAatat                                                     <  2:4524482/150‑1 (MQ=255)
                                                                                                  aaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGACATATTCAACTCATTGCGTGCAATAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTTAACTAATGGTAGAAATGTCGAATAATAatat                                                     >  2:1964586/1‑150 (MQ=255)
                                                                                                   aTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAAtata                                                    >  2:865163/1‑150 (MQ=255)
                                                                                                    tAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaa                                                   >  1:2764881/1‑150 (MQ=255)
                                                                                                      gTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaaaa                                                 >  1:99892/1‑150 (MQ=255)
                                                                                                       taaataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTCGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTTATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAg                                                <  1:335547/150‑1 (MQ=255)
                                                                                                       taaataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAg                                                <  1:2730965/150‑1 (MQ=255)
                                                                                                        aaataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGTGAGAAATGTCGAATAATAATATAAAAGt                                               >  2:573146/1‑150 (MQ=255)
                                                                                                         aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGtt                                              <  1:122315/150‑1 (MQ=255)
                                                                                                         aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGtt                                              >  2:3411965/1‑150 (MQ=255)
                                                                                                         aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGtt                                              >  2:4364629/1‑150 (MQ=255)
                                                                                                             aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAtt                                          >  2:3235425/1‑150 (MQ=255)
                                                                                                             aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAtt                                          >  2:243364/1‑150 (MQ=255)
                                                                                                             aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAtt                                          >  1:1477769/1‑150 (MQ=255)
                                                                                                               aaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTa                                        >  2:1880675/1‑150 (MQ=255)
                                                                                                               aaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAAGATAAAAGTTCATGTa                                        >  2:1081255/1‑150 (MQ=255)
                                                                                                                aaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTaa                                       >  1:3746618/1‑150 (MQ=255)
                                                                                                                  aataatAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAAt                                     <  1:738146/150‑1 (MQ=255)
                                                                                                                  aataatAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAAt                                     >  2:744798/1‑150 (MQ=255)
                                                                                                                   ataataAACTACTAATAATTTGCGTCATATTCATCTCCTTGCGTGCACTAGGCTATGTTAACGAAATTTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATTAAAGTTCATTTAAATg                                    <  1:1526604/150‑1 (MQ=255)
                                                                                                                    taataaACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGa                                   >  2:4052649/1‑150 (MQ=255)
                                                                                                                     aataaACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGaa                                  >  2:136156/1‑150 (MQ=255)
                                                                                                                      ataaACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAc                                 <  1:3069808/150‑1 (MQ=255)
                                                                                                                      ataaACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAc                                 <  1:3461815/150‑1 (MQ=255)
                                                                                                                       taaACTACAAATAAAATGCGTCATATTCACCTCAGTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAcc                                <  2:774773/150‑1 (MQ=255)
                                                                                                                         aaCTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCag                              >  1:3142019/1‑150 (MQ=255)
                                                                                                                           cTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCagag                            >  1:4608408/1‑150 (MQ=255)
                                                                                                                           cTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCagag                            >  2:1585491/1‑150 (MQ=255)
                                                                                                                            tACAAATAAAATGCGTCATAATCAACTCATAGCGTGCAATAGTCAATGATAAAGAAAATTCACTAAAGCGTGTCATAAATAGTTTAAATTCTGAACTCATCGGAGAAATGTCGAAAAATAATATAAAAGTTCATTTAAATCAACCAtaga                           >  2:4409849/1‑150 (MQ=255)
                                                                                                                             aCAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGat                          >  2:2822095/1‑150 (MQ=255)
                                                                                                                              cAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGata                         >  1:1814763/1‑150 (MQ=255)
                                                                                                                               aaaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATTAACCAGAGataa                        >  1:2308117/1‑150 (MQ=255)
                                                                                                                               aaaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataa                        >  1:683742/1‑150 (MQ=255)
                                                                                                                                   aaaaTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtt                    >  1:424837/1‑150 (MQ=255)
                                                                                                                                    aaaTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt                   <  2:2588620/150‑1 (MQ=255)
                                                                                                                                    aaaTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt                   >  1:1674964/1‑150 (MQ=255)
                                                                                                                                    aaaTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt                   >  1:1730140/1‑150 (MQ=255)
                                                                                                                                     aaTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtttt                  <  2:313036/150‑1 (MQ=255)
                                                                                                                                      aTGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttttt                 <  2:1336630/150‑1 (MQ=255)
                                                                                                                                             atatTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGcc          >  2:2904150/1‑150 (MQ=255)
                                                                                                                                             atatTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGcc          <  1:3255322/150‑1 (MQ=255)
                                                                                                                                               atTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGa        >  2:391821/1‑150 (MQ=255)
                                                                                                                                               atTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGa        >  1:2209919/1‑150 (MQ=255)
                                                                                                                                                 tCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATc      <  2:2211695/150‑1 (MQ=255)
                                                                                                                                                   aaCTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCtt    <  1:2029882/150‑1 (MQ=255)
                                                                                                                                                   aaCTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCtt    >  1:1780694/1‑150 (MQ=255)
                                                                                                                                                   aaCTCATTGCGTGCACTAGGATATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGACTAATAATATAACAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCtt    <  1:2239991/150‑1 (MQ=255)
                                                                                                                                                     cTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCa  >  1:3521868/1‑150 (MQ=255)
                                                                                                                                                     cTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCa  >  1:2032374/1‑150 (MQ=255)
                                                                                                                                                     cTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCa  >  1:1014557/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCA  >  CP009273/2376810‑2377108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A