Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,395,151 C→A 15.0% T127T (ACG→ACT)  nuoE ← NADH:ubiquinone oxidoreductase, chain E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,395,1510CA15.0% 819.0 / 85.5 252T127T (ACG→ACT) nuoENADH:ubiquinone oxidoreductase, chain E
Reads supporting (aligned to +/- strand):  ref base C (101/113);  new base A (17/21);  total (118/134)
Fisher's exact test for biased strand distribution p-value = 8.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 263 total aligned reads displayed.

ATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCTG  >  CP009273/2395002‑2395300
                                                                                                                                                     |                                                                                                                                                     
aTGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCagcagc                                                                                                                                                       >  2:3511968/1‑150 (MQ=255)
aTGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCagcagc                                                                                                                                                       <  2:4304292/150‑1 (MQ=255)
 tGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCg                                                                                                                                                      <  1:2990204/150‑1 (MQ=255)
  gCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGt                                                                                                                                                     <  1:73936/150‑1 (MQ=255)
      ttCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAg                                                                                                                                                 <  1:2233288/150‑1 (MQ=255)
       tCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGc                                                                                                                                                >  2:2832641/1‑150 (MQ=255)
        cGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCATCAAGTTGGCAGCAGCGTAAAGCg                                                                                                                                               <  1:4418599/150‑1 (MQ=255)
            aGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCa                                                                                                                                           <  1:3252664/150‑1 (MQ=255)
             gTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCAt                                                                                                                                          <  2:3347707/150‑1 (MQ=255)
                     aaTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTc                                                                                                                                  >  2:2978541/1‑150 (MQ=255)
                     aaTGTTTTTCATTTAGACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTc                                                                                                                                  <  1:537228/150‑1 (MQ=255)
                      aTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCg                                                                                                                                 >  1:1741800/1‑150 (MQ=255)
                        gTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGtt                                                                                                                               >  2:3663581/1‑150 (MQ=255)
                        gTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGtt                                                                                                                               >  1:521102/1‑150 (MQ=255)
                        gTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGtt                                                                                                                               >  1:3690784/1‑150 (MQ=255)
                         tttttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGttt                                                                                                                              >  2:4252627/1‑150 (MQ=255)
                         tttttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGttt                                                                                                                              <  2:4089577/150‑1 (MQ=255)
                          ttttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTg                                                                                                                             <  2:1126077/150‑1 (MQ=255)
                          ttttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTg                                                                                                                             <  2:3024315/150‑1 (MQ=255)
                          ttttCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTg                                                                                                                             >  1:1591846/1‑150 (MQ=255)
                                tttATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTgg                                                                                                                       <  2:2439317/150‑1 (MQ=255)
                                  tataCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGtt                                                                                                                     >  2:3871202/1‑150 (MQ=255)
                                    taCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg                                                                                                                   <  2:1089029/150‑1 (MQ=255)
                                    taCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg                                                                                                                   >  2:2939366/1‑150 (MQ=255)
                                      ccGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAt                                                                                                                 >  2:2761785/1‑150 (MQ=255)
                                           ccAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCa                                                                                                            <  2:509359/150‑1 (MQ=255)
                                              gcagTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCt                                                                                                         >  2:3949899/1‑150 (MQ=255)
                                                 gTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCtttt                                                                                                      <  2:1315826/150‑1 (MQ=255)
                                                 gTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCtttt                                                                                                      <  1:3733881/150‑1 (MQ=255)
                                                  ttCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGGTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGTGGCCATCAAATGTCGTTTGCCCTGGTTTGATGATCAGCatttt                                                                                                     >  2:3445305/1‑150 (MQ=255)
                                                   tCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtc                                                                                                    >  2:1262047/1‑150 (MQ=255)
                                                     aGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGGTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtctc                                                                                                  <  1:3157007/150‑1 (MQ=255)
                                                       ggATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGa                                                                                                <  2:1135418/150‑1 (MQ=255)
                                                         aTCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgc                                                                                              <  1:4262483/150‑1 (MQ=255)
                                                          tCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcg                                                                                             <  2:2255024/150‑1 (MQ=255)
                                                          tCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcg                                                                                             <  1:1074387/150‑1 (MQ=255)
                                                            gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgcc                                                                                           <  1:4585179/150‑1 (MQ=255)
                                                             cTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg                                                                                          >  2:3599814/1‑150 (MQ=255)
                                                              ttCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccgc                                                                                         >  1:828329/1‑150 (MQ=255)
                                                                ccGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCt                                                                                       <  1:1285576/150‑1 (MQ=255)
                                                                  ggggTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGa                                                                                     >  2:3129371/1‑150 (MQ=255)
                                                                  ggggTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGa                                                                                     >  1:2902957/1‑150 (MQ=255)
                                                                     gTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATa                                                                                  <  2:962405/150‑1 (MQ=255)
                                                                          aTGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTg                                                                             <  1:1518450/150‑1 (MQ=255)
                                                                           tGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGa                                                                            >  1:2627367/1‑150 (MQ=255)
                                                                                  gAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt                                                                     >  2:186712/1‑150 (MQ=255)
                                                                                   aGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGtt                                                                    <  2:2489765/150‑1 (MQ=255)
                                                                                    gtgtCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg                                                                   <  1:2984619/150‑1 (MQ=255)
                                                                                      gtCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGat                                                                 <  2:4518299/150‑1 (MQ=255)
                                                                                       tCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGata                                                                <  1:2712229/150‑1 (MQ=255)
                                                                                       tCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGata                                                                >  1:1909862/1‑150 (MQ=255)
                                                                                           cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGTGCCGCCTGAATACCCTGATAACCGTTGATATGAc                                                            >  1:15956/1‑150 (MQ=255)
                                                                                           cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGTGCCGCCTGAATACCCTGATAACCGTTGATATGAc                                                            >  1:2324295/1‑150 (MQ=255)
                                                                                           cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGAc                                                            >  2:2195940/1‑150 (MQ=255)
                                                                                             tCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAg                                                          >  1:953232/1‑150 (MQ=255)
                                                                                                atcatcATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGAcc                                                       >  2:836342/1‑150 (MQ=255)
                                                                                                 tcatcaTGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCa                                                      >  1:2173545/1‑150 (MQ=255)
                                                                                                 tcatcaTGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCa                                                      <  2:2343205/150‑1 (MQ=255)
                                                                                                  catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTAGATATGACAGACCAc                                                     >  2:3039863/1‑150 (MQ=255)
                                                                                                     catGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCt                                                  >  2:588873/1‑150 (MQ=255)
                                                                                                     catGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCt                                                  >  2:2682436/1‑150 (MQ=255)
                                                                                                        gTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTc                                               >  1:2780602/1‑150 (MQ=255)
                                                                                                        gTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTc                                               >  1:3833292/1‑150 (MQ=255)
                                                                                                        gTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTc                                               >  1:2675191/1‑150 (MQ=255)
                                                                                                        gTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTc                                               <  1:932448/150‑1 (MQ=255)
                                                                                                          ttGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTcac                                             >  1:923578/1‑150 (MQ=255)
                                                                                                             gCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAAt                                          <  1:818068/150‑1 (MQ=255)
                                                                                                              cccTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATa                                         <  1:248564/150‑1 (MQ=255)
                                                                                                              cccTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATa                                         <  1:2070347/150‑1 (MQ=255)
                                                                                                                  ttATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACgg                                     <  1:3477175/150‑1 (MQ=255)
                                                                                                                   tATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGa                                    <  2:4535994/150‑1 (MQ=255)
                                                                                                                     tCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATc                                  <  2:1030839/150‑1 (MQ=255)
                                                                                                                       acaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATcac                                <  2:4290158/150‑1 (MQ=255)
                                                                                                                       acaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATcac                                <  2:4308177/150‑1 (MQ=255)
                                                                                                                         aGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACAt                              <  1:4536325/150‑1 (MQ=255)
                                                                                                                         aGTTCCCCAGGCAGCAAGTTGGCAGAAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACTCTGTCACAATAACGGATAACAt                              >  1:3133336/1‑150 (MQ=255)
                                                                                                                          gTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATg                             >  2:1573897/1‑150 (MQ=255)
                                                                                                                           ttCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAATCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATgg                            >  2:3995944/1‑150 (MQ=255)
                                                                                                                             ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCg                          <  2:3241798/150‑1 (MQ=255)
                                                                                                                              cccAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGa                         >  1:3586568/1‑150 (MQ=255)
                                                                                                                               ccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGAc                        <  2:3697513/150‑1 (MQ=255)
                                                                                                                               ccAGGCAGCAAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGAc                        >  2:4176028/1‑150 (MQ=255)
                                                                                                                                 aGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCa                      >  1:1251862/1‑150 (MQ=255)
                                                                                                                                    cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAAcc                   >  1:3930740/1‑150 (MQ=255)
                                                                                                                                    cagcaAGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAAcc                   >  2:1283409/1‑150 (MQ=255)
                                                                                                                                     agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCg                  >  2:2190549/1‑150 (MQ=255)
                                                                                                                                        aaGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCt               <  1:1970164/150‑1 (MQ=255)
                                                                                                                                        aaGTTGGCAGCAGCGTAAAGCGGCCATCAAAGGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAGACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCt               <  2:1394084/150‑1 (MQ=255)
                                                                                                                                        aaGTTGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCt               >  2:2378510/1‑150 (MQ=255)
                                                                                                                                          gTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTgg             <  1:682371/150‑1 (MQ=255)
                                                                                                                                            tGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCg           >  1:650025/1‑150 (MQ=255)
                                                                                                                                            tGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCg           >  1:4063841/1‑150 (MQ=255)
                                                                                                                                            tGGCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCg           <  2:18297/150‑1 (MQ=255)
                                                                                                                                             ggCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATTACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCgg          >  1:42154/1‑150 (MQ=255)
                                                                                                                                             ggCAGCAGAGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCgg          >  2:2070438/1‑150 (MQ=255)
                                                                                                                                              gcagcaGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGa         <  1:3992177/150‑1 (MQ=255)
                                                                                                                                              gcagcaGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGa         <  1:207410/150‑1 (MQ=255)
                                                                                                                                                agcagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAg       <  1:3070222/150‑1 (MQ=255)
                                                                                                                                                    gcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCt   <  2:2779850/150‑1 (MQ=255)
                                                                                                                                                     cGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCTg  <  1:1107685/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCTG  >  CP009273/2395002‑2395300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A