Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,417,358 C→A 17.1% E211* (GAA→TAA)  hisP ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,417,3580CA17.1% 858.8 / 115.8 280E211* (GAA→TAA) hisPhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (110/122);  new base A (20/28);  total (130/150)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 284 total aligned reads displayed.

AGGAAATTATTTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCCGCGCGATAGA  >  CP009273/2417209‑2417505
                                                                                                                                                     |                                                                                                                                                   
agGAAATTATTTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTc                                                                                                                                                     >  2:4184936/1‑150 (MQ=255)
agGAAATTATTTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTc                                                                                                                                                     >  2:1384945/1‑150 (MQ=255)
    aaTTATTTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGa                                                                                                                                                 <  1:3411248/150‑1 (MQ=255)
     attattTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAg                                                                                                                                                >  1:369425/1‑150 (MQ=255)
        attTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCa                                                                                                                                             >  2:387977/1‑150 (MQ=255)
        attTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCa                                                                                                                                             >  1:221763/1‑150 (MQ=255)
          ttCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACt                                                                                                                                           <  1:4428979/150‑1 (MQ=255)
            cAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTac                                                                                                                                         <  1:2865045/150‑1 (MQ=255)
             aGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTCTTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTacc                                                                                                                                        <  2:3302574/150‑1 (MQ=255)
             aGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTacc                                                                                                                                        >  2:384356/1‑150 (MQ=255)
              gCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTacca                                                                                                                                       <  2:4410636/150‑1 (MQ=255)
              gCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTacca                                                                                                                                       <  2:3674928/150‑1 (MQ=255)
               cGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTaccac                                                                                                                                      <  2:2524369/150‑1 (MQ=255)
                  tCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCAt                                                                                                                                   >  2:2753914/1‑150 (MQ=255)
                   cccTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATg                                                                                                                                  <  1:1528880/150‑1 (MQ=255)
                    ccTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATgg                                                                                                                                 <  2:2828061/150‑1 (MQ=255)
                    ccTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATgg                                                                                                                                 <  2:1341882/150‑1 (MQ=255)
                     cTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGt                                                                                                                                >  2:3535013/1‑150 (MQ=255)
                        aaGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGtttt                                                                                                                             <  2:2004329/150‑1 (MQ=255)
                        aaGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGtttt                                                                                                                             <  2:1047580/150‑1 (MQ=255)
                         aGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTc                                                                                                                            <  1:1044506/150‑1 (MQ=255)
                         aGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTc                                                                                                                            >  1:2429349/1‑150 (MQ=255)
                         aGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTc                                                                                                                            >  1:1655064/1‑150 (MQ=255)
                          ggAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTcc                                                                                                                           <  2:41392/150‑1 (MQ=255)
                             aCCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCct                                                                                                                        >  2:1789731/1‑150 (MQ=255)
                             aCCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCct                                                                                                                        <  2:1010493/150‑1 (MQ=255)
                             aCCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCct                                                                                                                        >  1:1537340/1‑150 (MQ=255)
                              ccGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCctc                                                                                                                       <  2:2323900/150‑1 (MQ=255)
                                gTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCtctt                                                                                                                     >  1:1113967/1‑150 (MQ=255)
                                  tGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCtcttct                                                                                                                   >  2:2970210/1‑150 (MQ=255)
                                    cAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGc                                                                                                                 >  2:4298628/1‑150 (MQ=255)
                                    cAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGc                                                                                                                 <  2:1444706/150‑1 (MQ=255)
                                     aGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGcc                                                                                                                >  1:2194056/1‑150 (MQ=255)
                                     aGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTACTGcc                                                                                                                >  1:2344369/1‑150 (MQ=255)
                                         gAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGtt                                                                                                            <  2:4617315/150‑1 (MQ=255)
                                          aGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTtg                                                                                                           <  2:1550098/150‑1 (MQ=255)
                                          aGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTtg                                                                                                           >  1:1301055/1‑150 (MQ=255)
                                              cTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTtgctgc                                                                                                       <  1:139660/150‑1 (MQ=255)
                                               tttGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCa                                                                                                      >  1:3122854/1‑150 (MQ=255)
                                                   cGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCataat                                                                                                  <  2:3061799/150‑1 (MQ=255)
                                                   cGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCataat                                                                                                  >  2:1288299/1‑150 (MQ=255)
                                                    gggTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCataata                                                                                                 >  2:3117949/1‑150 (MQ=255)
                                                    gggTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCataata                                                                                                 >  2:2717069/1‑150 (MQ=255)
                                                     ggTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATAc                                                                                                <  2:2503939/150‑1 (MQ=255)
                                                     ggTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATAc                                                                                                <  1:355923/150‑1 (MQ=255)
                                                     ggTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATAc                                                                                                <  2:2613662/150‑1 (MQ=255)
                                                      gTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACg                                                                                               <  2:1305090/150‑1 (MQ=255)
                                                       ttGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGc                                                                                              >  2:4473641/1‑150 (MQ=255)
                                                               tAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTc                                                                                      <  2:2298514/150‑1 (MQ=255)
                                                                    gCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTa                                                                                 <  1:1825208/150‑1 (MQ=255)
                                                                    gCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTa                                                                                 >  2:625763/1‑150 (MQ=255)
                                                                       ccGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCa                                                                              <  1:264593/150‑1 (MQ=255)
                                                                         ggCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGt                                                                            >  2:987072/1‑150 (MQ=255)
                                                                         ggCGCGCCCTCTTCATCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGt                                                                            >  1:3153562/1‑150 (MQ=255)
                                                                          gcgcgcCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGtt                                                                           <  1:1016319/150‑1 (MQ=255)
                                                                          gcgcgcCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGtt                                                                           <  1:2726839/150‑1 (MQ=255)
                                                                            gcgcCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCa                                                                         >  1:689163/1‑150 (MQ=255)
                                                                               cccTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGa                                                                      >  1:208098/1‑150 (MQ=255)
                                                                                ccTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGAt                                                                     >  1:2463833/1‑150 (MQ=255)
                                                                                 ctctTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATc                                                                    >  1:3231531/1‑150 (MQ=255)
                                                                                 ctctTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATc                                                                    >  2:2154219/1‑150 (MQ=255)
                                                                                 ctctTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATc                                                                    <  2:3404704/150‑1 (MQ=255)
                                                                                   cttcttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGa                                                                  <  2:4523151/150‑1 (MQ=255)
                                                                                   cttcttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGa                                                                  <  1:3326999/150‑1 (MQ=255)
                                                                                   cttcttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGa                                                                  <  1:884409/150‑1 (MQ=255)
                                                                                     tcttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAATCATGGCGAGCAAAGACCATTTCGTGAGTCACTACCACCATGTTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCATCACTTCGCCTACCAGTTCAGGATCGAgc                                                                <  1:404186/150‑1 (MQ=255)
                                                                                     tcttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAgc                                                                <  2:2072419/150‑1 (MQ=255)
                                                                                      cttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTATTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAgcg                                                               >  2:4497787/1‑150 (MQ=255)
                                                                                      cttctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAgcg                                                               <  1:2991692/150‑1 (MQ=255)
                                                                                         ctATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCg                                                            <  1:3643415/150‑1 (MQ=255)
                                                                                          tATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGa                                                           <  1:1227573/150‑1 (MQ=255)
                                                                                           aTTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGag                                                          >  2:1887005/1‑150 (MQ=255)
                                                                                           aTTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGag                                                          <  2:476816/150‑1 (MQ=255)
                                                                                            ttttCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGagg                                                         <  1:4150020/150‑1 (MQ=255)
                                                                                                 cccTGATTGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGaggtaggt                                                    <  2:2716691/150‑1 (MQ=255)
                                                                                                   cTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTtc                                                  >  1:1718139/1‑150 (MQ=255)
                                                                                                    tGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTtca                                                 >  2:2813767/1‑150 (MQ=255)
                                                                                                           ggaAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACa                                          <  2:458909/150‑1 (MQ=255)
                                                                                                            gaAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAg                                         <  1:3656713/150‑1 (MQ=255)
                                                                                                            gaAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAg                                         >  1:3441622/1‑150 (MQ=255)
                                                                                                               aaTGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTaa                                      <  2:2571239/150‑1 (MQ=255)
                                                                                                                 tGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTaaaa                                    >  2:789338/1‑150 (MQ=255)
                                                                                                                    cATGAGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACtt                                 <  2:803345/150‑1 (MQ=255)
                                                                                                                        aGTAGAAACATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCgg                             >  1:3505091/1‑150 (MQ=255)
                                                                                                                         gTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGt                            >  1:3916544/1‑150 (MQ=255)
                                                                                                                               aCATGGCGAGCAAAGCCCATTTAGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATc                      <  2:2434270/150‑1 (MQ=255)
                                                                                                                                cATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCg                     <  2:3594244/150‑1 (MQ=255)
                                                                                                                                cATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCg                     <  2:1275098/150‑1 (MQ=255)
                                                                                                                                 aTGGAGAGCAAATCCCATATCTTGAGTCACAACAACCAAGGTTATCCCCTCAGCTGCCAGTTGCTGCATAATACGAAACACTTCTCATACCAGTACAGGATCGATCGCAGAGGTAGGTTCATCAAACACTAAAAATTCCGGTTCCAACga                    >  2:4033719/1‑149 (MQ=255)
                                                                                                                                  tGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGcc                   >  2:493947/1‑150 (MQ=255)
                                                                                                                                  tGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGcc                   >  1:2872446/1‑150 (MQ=255)
                                                                                                                                    gCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAg                 <  2:1694461/150‑1 (MQ=255)
                                                                                                                                        gCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGcc             >  2:1524051/1‑150 (MQ=255)
                                                                                                                                         cAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGccc            <  2:507003/150‑1 (MQ=255)
                                                                                                                                         aaaaGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGccc            <  1:25587/149‑1 (MQ=255)
                                                                                                                                            aGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCcgcg         >  1:822862/1‑150 (MQ=255)
                                                                                                                                            aGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCcgcg         >  1:84275/1‑150 (MQ=255)
                                                                                                                                              cccATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCcgcgcg       >  1:4582745/1‑150 (MQ=255)
                                                                                                                                                  tttCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTNCCGGTTCCATCGCCAGCGCCCGCGCGATAg   <  1:4278961/150‑1 (MQ=255)
                                                                                                                                                   ttCGTGAGTCACTACCACCATGGTGTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCCGCGCGATAGa  <  2:3230010/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGGAAATTATTTCAGCGATCCCTTAAGGAACCGTTGCAGACGAGGGCTTTGCGGGTTGCCAAATAACTGCTCCGGCGCGCCCTCTTCTTCTATTTTCCCCTGATGGAGGAAAATGACATGAGTAGAAACATGGCGAGCAAAGCCCATTTCGTGAGTCACTACCACCATGGTTTTCCCCTCTTCTGCCAGTTGCTGCATAATACGCAACACTTCGCCTACCAGTTCAGGATCGAGCGCCGAGGTAGGTTCATCAAACAGTAAAACTTCCGGTTCCATCGCCAGCGCCCGCGCGATAGA  >  CP009273/2417209‑2417505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A