Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,516,301 G→T 44.7% L348L (CTC→CTA)  xapB ← xanthosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,516,3010GT44.7% 238.3 / inf 272L348L (CTC→CTA) xapBxanthosine transporter
Reads supporting (aligned to +/- strand):  ref base G (69/81);  new base T (62/60);  total (131/141)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

AATATCACTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAG  >  CP009273/2516152‑2516448
                                                                                                                                                     |                                                                                                                                                   
  tatCACTGCGAGAAATAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAagag                                                                                                                                                   <  2:1030025/150‑1 (MQ=255)
    tCACTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGcc                                                                                                                                                 <  1:3177791/150‑1 (MQ=255)
     cACTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGccc                                                                                                                                                >  2:514940/1‑150 (MQ=255)
      aCTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGcccc                                                                                                                                               >  1:2159469/1‑150 (MQ=255)
      aCTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGcccc                                                                                                                                               <  1:3119841/150‑1 (MQ=255)
      aCTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGcccc                                                                                                                                               <  2:2160528/150‑1 (MQ=255)
       cTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCt                                                                                                                                              >  2:3201904/1‑150 (MQ=255)
          cGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTgcg                                                                                                                                           >  2:3440404/1‑150 (MQ=255)
           gagaAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTgcgc                                                                                                                                          >  2:561887/1‑150 (MQ=255)
           gagaAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACACAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTgcgc                                                                                                                                          >  2:2815672/1‑150 (MQ=255)
            agaAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTgcgcg                                                                                                                                         <  1:1458005/150‑1 (MQ=255)
            agaAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTgcgcg                                                                                                                                         <  2:74486/150‑1 (MQ=255)
              aaaaaGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCt                                                                                                                                       <  2:2733270/150‑1 (MQ=255)
               aaaaGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTg                                                                                                                                      >  1:1078981/1‑150 (MQ=255)
                aaaGAGCATATCCTGCAAACACCAGCCAGATAGTATGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTgg                                                                                                                                     >  2:2928335/1‑150 (MQ=255)
                   gagCATATCCTGCAATCACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCAc                                                                                                                                  <  1:4397816/150‑1 (MQ=255)
                       atatCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAAt                                                                                                                              >  1:250146/1‑150 (MQ=255)
                       atatCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAAt                                                                                                                              >  2:1685762/1‑150 (MQ=255)
                       atatCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAAt                                                                                                                              <  1:1845297/150‑1 (MQ=255)
                       atatCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAAt                                                                                                                              <  2:2857910/150‑1 (MQ=255)
                         atCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGc                                                                                                                            >  1:3943415/1‑150 (MQ=255)
                                 aaCACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCa                                                                                                                    <  1:389594/150‑1 (MQ=255)
                                   cacCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAAc                                                                                                                  <  2:1194097/150‑1 (MQ=255)
                                    acCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACt                                                                                                                 >  2:4439090/1‑150 (MQ=255)
                                    acCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACt                                                                                                                 <  2:3896203/150‑1 (MQ=255)
                                    acCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACt                                                                                                                 >  1:4038486/1‑150 (MQ=255)
                                    acCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACt                                                                                                                 <  1:4496693/150‑1 (MQ=255)
                                      cagccagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTc                                                                                                               <  1:2423918/150‑1 (MQ=255)
                                       agccagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTcc                                                                                                              <  1:4014521/150‑1 (MQ=255)
                                         ccagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTg                                                                                                            <  1:4203335/150‑1 (MQ=255)
                                         ccagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTg                                                                                                            >  1:3706555/1‑150 (MQ=255)
                                          cagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGt                                                                                                           >  2:19677/1‑150 (MQ=255)
                                          cagATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGt                                                                                                           >  2:19665/1‑150 (MQ=255)
                                           agATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGtt                                                                                                          >  2:798586/1‑150 (MQ=255)
                                            gATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTc                                                                                                         <  1:2945678/150‑1 (MQ=255)
                                             aTAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCg                                                                                                        <  1:596048/150‑1 (MQ=255)
                                              tAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGa                                                                                                       >  1:2981606/1‑150 (MQ=255)
                                               aGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGAc                                                                                                      >  2:2085708/1‑150 (MQ=255)
                                                 tttGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACaa                                                                                                    <  2:1580142/150‑1 (MQ=255)
                                                   tGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAAt                                                                                                  >  2:1360624/1‑150 (MQ=255)
                                                     ccAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATAc                                                                                                >  2:408004/1‑150 (MQ=255)
                                                          cTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAAc                                                                                           <  2:2783354/150‑1 (MQ=255)
                                                            tttACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCa                                                                                         <  1:650849/150‑1 (MQ=255)
                                                            tttACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCa                                                                                         <  1:832249/150‑1 (MQ=255)
                                                             ttACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAg                                                                                        >  2:2006478/1‑150 (MQ=255)
                                                              tACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGa                                                                                       <  2:4137219/150‑1 (MQ=255)
                                                              tACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGa                                                                                       <  2:1091750/150‑1 (MQ=255)
                                                               aCGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGTGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGaa                                                                                      >  2:3669176/1‑150 (MQ=255)
                                                               aCGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGaa                                                                                      <  1:1723314/150‑1 (MQ=255)
                                                                 gCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAat                                                                                    >  1:230589/1‑150 (MQ=255)
                                                                    aTCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATAtt                                                                                 >  1:1967184/1‑150 (MQ=255)
                                                                         ccGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTgaaga                                                                            <  2:1430057/150‑1 (MQ=255)
                                                                          cGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTgaagaa                                                                           <  1:912634/150‑1 (MQ=255)
                                                                           gAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGaaa                                                                          >  1:459753/1‑150 (MQ=255)
                                                                           gAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGaaa                                                                          >  2:3758603/1‑150 (MQ=255)
                                                                           gAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGaaa                                                                          >  2:3232309/1‑150 (MQ=255)
                                                                             aaaGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATc                                                                        >  2:3140354/1‑150 (MQ=255)
                                                                               aGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGa                                                                      >  2:3692783/1‑150 (MQ=255)
                                                                                gTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGaa                                                                     >  1:3181171/1‑150 (MQ=255)
                                                                                 tAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCTTGCGCCGACACCATTTACCATGGTCTTACATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGTAATTTTGAAGAAATCGAAt                                                                    <  2:3340966/150‑1 (MQ=255)
                                                                                 tAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAAt                                                                    <  1:340986/150‑1 (MQ=255)
                                                                                    tCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGca                                                                 <  2:90667/150‑1 (MQ=255)
                                                                                     cTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGcac                                                                <  2:3656233/150‑1 (MQ=255)
                                                                                      tACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGcaca                                                               >  1:2566902/1‑150 (MQ=255)
                                                                                       aCTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAg                                                              <  1:2663024/150‑1 (MQ=255)
                                                                                          gccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCa                                                           <  2:4388738/150‑1 (MQ=255)
                                                                                          gccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCa                                                           >  2:3677555/1‑150 (MQ=255)
                                                                                           ccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCAt                                                          >  1:1285456/1‑150 (MQ=255)
                                                                                           ccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCAt                                                          <  1:780149/150‑1 (MQ=255)
                                                                                            catgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATa                                                         >  1:167267/1‑150 (MQ=255)
                                                                                               gccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAAc                                                      >  2:2349550/1‑150 (MQ=255)
                                                                                               gccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAAc                                                      <  1:2259271/150‑1 (MQ=255)
                                                                                                 caCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACaa                                                    >  2:674831/1‑150 (MQ=255)
                                                                                                 caCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACaa                                                    >  1:4097859/1‑150 (MQ=255)
                                                                                                  aCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAat                                                   <  1:457094/150‑1 (MQ=255)
                                                                                                  aCTCAGAATCGAGCCAACCCATGCGCCGACACAATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCAAGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAat                                                   <  2:4000461/150‑1 (MQ=255)
                                                                                                     cAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAatcat                                                <  2:2499516/150‑1 (MQ=255)
                                                                                                      aGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAatcatc                                               >  2:2271348/1‑150 (MQ=255)
                                                                                                       gAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCg                                              >  2:4260163/1‑150 (MQ=255)
                                                                                                         aTCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGAc                                            <  1:470872/150‑1 (MQ=255)
                                                                                                            gAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGAcagc                                         >  1:3332544/1‑150 (MQ=255)
                                                                                                            gAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGAcagc                                         >  2:1771454/1‑150 (MQ=255)
                                                                                                               ccAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGAcagcagc                                      >  2:1901383/1‑150 (MQ=255)
                                                                                                                       tGCGCCGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAaaat                              <  1:1106780/150‑1 (MQ=255)
                                                                                                                           ccGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAaaataaat                          >  2:2626757/1‑150 (MQ=255)
                                                                                                                           ccGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAaaataaat                          <  2:24126/150‑1 (MQ=255)
                                                                                                                           ccGACACCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAaaataaat                          <  1:3596946/150‑1 (MQ=255)
                                                                                                                               cacCATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCgg                      >  2:4575868/1‑150 (MQ=255)
                                                                                                                                 ccATTTACCATGGTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgtt                    <  2:2290672/150‑1 (MQ=255)
                                                                                                                                   aTTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgt                  <  1:383940/150‑1 (MQ=255)
                                                                                                                                   aTTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgt                  <  1:1619568/150‑1 (MQ=255)
                                                                                                                                   aTTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgt                  <  1:1432383/150‑1 (MQ=255)
                                                                                                                                    tttACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgtt                 >  2:947393/1‑150 (MQ=255)
                                                                                                                                        ccATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACg             <  2:4316824/150‑1 (MQ=255)
                                                                                                                                         cATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACgg            <  1:2349743/150‑1 (MQ=255)
                                                                                                                                          aTGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGa           <  2:1303976/150‑1 (MQ=255)
                                                                                                                                            ggTCATAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATc         <  2:2522265/150‑1 (MQ=255)
                                                                                                                                               cATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGcc      >  1:2790449/1‑150 (MQ=255)
                                                                                                                                                aTAAATAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCa     >  1:4605133/1‑150 (MQ=255)
                                                                                                                                                   aaGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAg  <  2:3836977/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATATCACTGCGAGAAAAAGAGCATATCCTGCAAACACCAGCCAGATAGTTTGCCAGTCTTTTACGCCATCCACCGAAAAGTAATCTACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAG  >  CP009273/2516152‑2516448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A