Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,044,437 T→A 49.9% intergenic (‑411/+37) gcvT ← / ← ubiI aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,044,4370TA49.9% 2.8 / inf 262intergenic (‑411/+37)gcvT/ubiIaminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (74/57);  new base A (72/59);  total (146/116)
Fisher's exact test for biased strand distribution p-value = 9.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TGAATAAGACAATATTAAGAGCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTT  >  CP009273/3044288‑3044586
                                                                                                                                                     |                                                                                                                                                     
tGAATAAGACAATATTAAGAGCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATACTGCGAAAACGGAGGTGAAATTAGTTTATTTCAATTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAgt                                                                                                                                                       >  2:2013452/1‑150 (MQ=255)
  aaTAAGACAATATTAAGAGCTAAAAAAATGTCAAAAAACACTAAAACAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAgtgt                                                                                                                                                     <  1:3515106/150‑1 (MQ=255)
      acaaaaTATTAAGAGCTAAAAAAATCTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATg                                                                                                                                                 <  2:3544629/146‑1 (MQ=255)
         cAATATTAAGAGCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGt                                                                                                                                              >  1:3625062/1‑150 (MQ=255)
          aaTCTTAAGAGCTAAAAAAACGTCACAACACACCAAATCAAAACATCAGGCCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGACAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTa                                                                                                                                             <  2:4380804/150‑1 (MQ=255)
             atTAAGAGCTAAAAAAATGTCAAAAAACACTACATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCa                                                                                                                                          <  1:210040/150‑1 (MQ=255)
                aaGAGCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAAt                                                                                                                                       >  1:1935028/1‑150 (MQ=255)
                 agagCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATACTGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATa                                                                                                                                      <  2:474788/150‑1 (MQ=255)
                     cTAAAAACATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAaaca                                                                                                                                  <  1:2893328/150‑1 (MQ=255)
                       aaaaaaaTGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAaacaac                                                                                                                                >  1:4635792/1‑150 (MQ=255)
                        aaaaaaTGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAacaaca                                                                                                                               >  1:2831507/1‑150 (MQ=255)
                        aaaaaaTGTCAAAAAACACTAAATAAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAacaaca                                                                                                                               <  2:2018253/150‑1 (MQ=255)
                         aaaaaTGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGTGTATCAAATAAACAACag                                                                                                                              >  1:425803/1‑150 (MQ=255)
                             aTGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACagaaga                                                                                                                          >  2:4110697/1‑150 (MQ=255)
                                 cAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGaaa                                                                                                                      >  1:1303032/1‑150 (MQ=255)
                                      aaCACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAAttttt                                                                                                                 >  1:3361401/1‑150 (MQ=255)
                                         acTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAAc                                                                                                              >  1:1058468/1‑150 (MQ=255)
                                         acTAAATCAAAAAATAATCGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAAc                                                                                                              <  1:1964562/150‑1 (MQ=255)
                                           tAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGc                                                                                                            >  1:3152712/1‑150 (MQ=255)
                                            aaaTCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCa                                                                                                           <  1:1747326/150‑1 (MQ=255)
                                            aaaTCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCa                                                                                                           >  2:2539358/1‑150 (MQ=255)
                                              aTCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGc                                                                                                         >  1:4311549/1‑150 (MQ=255)
                                               tCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGcc                                                                                                        >  2:141447/1‑150 (MQ=255)
                                                 aaaaaaTAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCAt                                                                                                      <  1:3753982/150‑1 (MQ=255)
                                                   aaaaTAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTc                                                                                                    >  2:1761082/1‑150 (MQ=255)
                                                    aaaTAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCa                                                                                                   <  2:2992919/150‑1 (MQ=255)
                                                    aaaTAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCa                                                                                                   <  2:4478961/150‑1 (MQ=255)
                                                     aataatGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTAACAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAg                                                                                                  >  1:4155413/1‑150 (MQ=255)
                                                      ataatGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAgg                                                                                                 >  1:2434114/1‑150 (MQ=255)
                                                       taatGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGc                                                                                                >  2:637432/1‑150 (MQ=255)
                                                        aatGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCa                                                                                               >  2:4397168/1‑150 (MQ=255)
                                                           ggCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAAt                                                                                            <  2:3353544/150‑1 (MQ=255)
                                                            gCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATc                                                                                           >  2:859488/1‑150 (MQ=255)
                                                            gCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATc                                                                                           >  2:1237661/1‑150 (MQ=255)
                                                               ttAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGtt                                                                                        <  2:2141214/150‑1 (MQ=255)
                                                                 aGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTa                                                                                      >  1:1234064/1‑150 (MQ=255)
                                                                 aGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTa                                                                                      >  1:4462262/1‑150 (MQ=255)
                                                                   aaaaTATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAAt                                                                                    >  2:1465400/1‑150 (MQ=255)
                                                                      atatAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATccc                                                                                 >  1:2454658/1‑150 (MQ=255)
                                                                        ataATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCAt                                                                               <  1:1324055/150‑1 (MQ=255)
                                                                         taATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCAtt                                                                              >  1:3001939/1‑150 (MQ=255)
                                                                         taATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCAtt                                                                              >  2:2494702/1‑150 (MQ=255)
                                                                          aaTGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTg                                                                             >  2:4043409/1‑150 (MQ=255)
                                                                          aaTGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTg                                                                             <  1:870170/150‑1 (MQ=255)
                                                                              cGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTg                                                                         >  1:3310445/1‑150 (MQ=255)
                                                                              cGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTg                                                                         <  1:1127737/150‑1 (MQ=255)
                                                                              cGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTg                                                                         <  1:1127819/150‑1 (MQ=255)
                                                                               gAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTgg                                                                        >  1:3896472/1‑150 (MQ=255)
                                                                                aaaaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGc                                                                       >  1:1274415/1‑150 (MQ=255)
                                                                                aaaaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGc                                                                       >  1:2292297/1‑150 (MQ=255)
                                                                                 aaaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCg                                                                      <  1:3300000/150‑1 (MQ=255)
                                                                                  aaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCgg                                                                     >  2:1582861/1‑150 (MQ=255)
                                                                                   aCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGa                                                                    >  1:645837/1‑150 (MQ=255)
                                                                                    cGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGAt                                                                   >  1:2199222/1‑150 (MQ=255)
                                                                                       aGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAg                                                                <  2:2373584/150‑1 (MQ=255)
                                                                                       aGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAg                                                                <  1:3619564/150‑1 (MQ=255)
                                                                                        ggTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGt                                                               >  1:1517378/1‑150 (MQ=255)
                                                                                        ggTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGt                                                               <  2:2826284/150‑1 (MQ=255)
                                                                                         gTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGtt                                                              <  1:699485/150‑1 (MQ=255)
                                                                                                 aGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTa                                                      >  2:3952767/1‑150 (MQ=255)
                                                                                                   tttATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTACCGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAAc                                                    <  2:873802/150‑1 (MQ=255)
                                                                                                   tttATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAAc                                                    <  1:160289/150‑1 (MQ=255)
                                                                                                   tttATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAAc                                                    <  2:3213357/150‑1 (MQ=255)
                                                                                                    ttATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACg                                                   >  1:280541/1‑150 (MQ=255)
                                                                                                       tttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCa                                                <  1:1642671/150‑1 (MQ=255)
                                                                                                        ttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAg                                               >  1:1811120/1‑150 (MQ=255)
                                                                                                        ttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAg                                               <  1:3903347/150‑1 (MQ=255)
                                                                                                          cAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGa                                             <  1:770324/150‑1 (MQ=255)
                                                                                                           aaaTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGaa                                            >  1:3349079/1‑150 (MQ=255)
                                                                                                            aaTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAg                                           <  2:3454192/150‑1 (MQ=255)
                                                                                                            aaTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAg                                           >  2:3879972/1‑150 (MQ=255)
                                                                                                             aTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGc                                          <  2:880319/150‑1 (MQ=255)
                                                                                                             aTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGc                                          >  1:2572124/1‑150 (MQ=255)
                                                                                                                aGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAAGTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgtg                                       <  2:4207877/150‑1 (MQ=255)
                                                                                                                  gAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTc                                     <  1:4003963/150‑1 (MQ=255)
                                                                                                                  gAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCAGTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTc                                     <  2:898323/150‑1 (MQ=255)
                                                                                                                   aaaaTCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATTCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCg                                    >  1:3262132/1‑150 (MQ=255)
                                                                                                                   aaaaTCTCCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCg                                    >  2:1160907/1‑150 (MQ=255)
                                                                                                                    aaaTCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCgg                                   >  2:4476150/1‑150 (MQ=255)
                                                                                                                     aaTCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGc                                  <  2:696061/150‑1 (MQ=255)
                                                                                                                       tctcCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCa                                <  1:2720001/150‑1 (MQ=255)
                                                                                                                       tctcCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCa                                <  2:3812353/150‑1 (MQ=255)
                                                                                                                        ctcCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAg                               <  1:3385125/150‑1 (MQ=255)
                                                                                                                         tcCCGGCGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGNGGCTTAACGCCAGGAAGCGTGTCGGCCAGt                              <  1:4281455/150‑1 (MQ=255)
                                                                                                                          cccGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGtt                             <  2:4132150/150‑1 (MQ=255)
                                                                                                                             ggCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCa                          >  1:1573413/1‑150 (MQ=255)
                                                                                                                              gCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCaa                         <  2:50357/150‑1 (MQ=255)
                                                                                                                               cGAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCaaa                        <  2:2284771/150‑1 (MQ=255)
                                                                                                                                gAAAAAACCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAc                       <  1:4466662/150‑1 (MQ=255)
                                                                                                                                  aaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCAAGGAAGCGTGTCGGCCAGTTTCAAACCa                     <  1:3807570/150‑1 (MQ=255)
                                                                                                                                  aaaaaCCGGGAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCa                     <  1:1455708/150‑1 (MQ=255)
                                                                                                                                         gggAGATGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc              <  1:550136/150‑1 (MQ=255)
                                                                                                                                           gagaTGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgc            >  2:1022827/1‑150 (MQ=255)
                                                                                                                                            agaTGAAAGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca           <  1:2146188/150‑1 (MQ=255)
                                                                                                                                            agaTGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca           <  1:1079583/150‑1 (MQ=255)
                                                                                                                                            agaTGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca           >  2:3899249/1‑150 (MQ=255)
                                                                                                                                              aTGAAAGAGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcagc         >  1:3324907/1‑150 (MQ=255)
                                                                                                                                                 aaaGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGt      >  1:3420968/1‑150 (MQ=255)
                                                                                                                                                  aaGTGTGATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtt     >  1:1009875/1‑150 (MQ=255)
                                                                                                                                                     tgtgATGGGTATCAAATAAACAACAGAAGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGttttt  >  2:3014925/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAATAAGACAATATTAAGAGCTAAAAAAATGTCAAAAAACACTAAATCAAAAAATAATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTT  >  CP009273/3044288‑3044586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A