Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,111,434 C→A 100% A346A (GCG→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,111,4340CA97.4% 787.8 / 10.0 234A346A (GCG→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (105/123);  total (109/125)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 234 total aligned reads displayed.

AGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACG  >  CP009273/3111288‑3111580
                                                                                                                                                  |                                                                                                                                                  
  cGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGcccc                                                                                                                                               >  2:1996425/1‑150 (MQ=255)
  cGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGcccc                                                                                                                                               <  2:2536128/150‑1 (MQ=255)
   gTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCACAGACCTTGCGTACATCGTCCGGAACAACACGGGGATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCcg                                                                                                                                              >  2:3804104/1‑150 (MQ=255)
   gTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCcg                                                                                                                                              >  2:3596340/1‑150 (MQ=255)
      gCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGa                                                                                                                                           >  2:1367193/1‑150 (MQ=255)
       cACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGaa                                                                                                                                          >  2:4473603/1‑150 (MQ=255)
        aCCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAAc                                                                                                                                         <  2:1128113/150‑1 (MQ=255)
        aCCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAAc                                                                                                                                         <  1:1549771/150‑1 (MQ=255)
            ttGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtca                                                                                                                                     >  2:3881684/1‑150 (MQ=255)
             tGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGTCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAATCTGATCAATATTAGCCCCGCGAACTtcat                                                                                                                                    <  2:2086842/150‑1 (MQ=255)
             tGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtcat                                                                                                                                    <  1:1444658/150‑1 (MQ=255)
                aTAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAc                                                                                                                                 >  1:2293943/1‑150 (MQ=255)
                 tAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                                >  2:3904494/1‑150 (MQ=255)
                 tAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                                <  1:978280/150‑1 (MQ=255)
                 tAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                                <  1:277934/150‑1 (MQ=255)
                  aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAccc                                                                                                                               >  1:1113458/1‑150 (MQ=255)
                    cGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAg                                                                                                                             <  1:777313/150‑1 (MQ=255)
                      agagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGtt                                                                                                                           >  2:4443715/1‑150 (MQ=255)
                          aTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagt                                                                                                                       >  1:580683/1‑150 (MQ=255)
                            tGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagtca                                                                                                                     <  1:2530694/150‑1 (MQ=255)
                            tGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagtca                                                                                                                     >  1:4413415/1‑150 (MQ=255)
                              aTAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAgc                                                                                                                   >  2:3993396/1‑150 (MQ=255)
                                aaTCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAgcgc                                                                                                                 >  1:2190159/1‑150 (MQ=255)
                                     cGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATgg                                                                                                            <  2:2502583/150‑1 (MQ=255)
                                            cACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACtt                                                                                                     <  2:554790/150‑1 (MQ=255)
                                               gttttgATATTCGGCTAAGACCTTGCGTACATCGTCCGGTACATCACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGTTCAATATTAGCCCCGCGATCTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAtt                                                                                                  <  2:2273343/145‑1 (MQ=255)
                                               gTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAtt                                                                                                  <  2:4603357/150‑1 (MQ=255)
                                                  gggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGTCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGcc                                                                                               <  2:1623996/150‑1 (MQ=255)
                                                     atatTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgcg                                                                                            >  1:3305467/1‑150 (MQ=255)
                                                      tatTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCgcgc                                                                                           >  1:4533212/1‑150 (MQ=255)
                                                       atTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCa                                                                                          >  1:2830873/1‑150 (MQ=255)
                                                         tCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACt                                                                                        <  1:2902960/150‑1 (MQ=255)
                                                         tCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACt                                                                                        <  2:1168131/150‑1 (MQ=255)
                                                          cGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTg                                                                                       <  1:250991/150‑1 (MQ=255)
                                                           ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                      <  1:4264376/150‑1 (MQ=255)
                                                               aaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAccc                                                                                  <  1:374342/150‑1 (MQ=255)
                                                                 gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                                <  2:4236056/150‑1 (MQ=255)
                                                                 gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                                >  2:4534595/1‑150 (MQ=255)
                                                                  aCCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGt                                                                               >  1:1010728/1‑150 (MQ=255)
                                                                   ccTTGCGTACATCTTCCGGAACAACACGGGTATTATTTTGACCGGTCGTCGAATATCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCAGCGCACTGAACCCAGtt                                                                              >  2:691403/1‑150 (MQ=255)
                                                                   ccTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGtt                                                                              <  1:1467299/150‑1 (MQ=255)
                                                                    cTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTc                                                                             <  1:3911357/150‑1 (MQ=255)
                                                                    cTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTc                                                                             >  2:3495085/1‑150 (MQ=255)
                                                                         gTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAgg                                                                        <  2:1222985/150‑1 (MQ=255)
                                                                         gTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAgg                                                                        <  2:2876409/150‑1 (MQ=255)
                                                                            cATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                     >  1:4318976/1‑150 (MQ=255)
                                                                            cATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                     <  1:4097949/150‑1 (MQ=255)
                                                                             aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGTCGCGCACTGAACCCAGTTCAAAGGTATc                                                                    >  1:3412019/1‑150 (MQ=255)
                                                                             aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATc                                                                    >  1:1361006/1‑150 (MQ=255)
                                                                             aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATc                                                                    <  1:520070/150‑1 (MQ=255)
                                                                             aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATc                                                                    <  1:3165060/150‑1 (MQ=255)
                                                                             aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATc                                                                    <  1:3591668/150‑1 (MQ=255)
                                                                                gTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTCTCGAt                                                                 >  2:406219/1‑150 (MQ=255)
                                                                                gTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGAt                                                                 <  2:1496656/150‑1 (MQ=255)
                                                                                gTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGAt                                                                 <  2:4596874/150‑1 (MQ=255)
                                                                                 tCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATa                                                                <  1:3112690/150‑1 (MQ=255)
                                                                                  ccGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATAc                                                               <  1:2086590/150‑1 (MQ=255)
                                                                                  cagtaacaaaACGGGTATTATTTTGCCCGGTCGACGAATAGCGATGAATAAGAAGATCAATATAAGCCCCGCGAAATTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCATATCAAAGGTATCGATAc                                                               >  2:3239141/5‑150 (MQ=255)
                                                                                     gAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaa                                                            >  1:3102135/1‑150 (MQ=255)
                                                                                      aacaacACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaaa                                                           <  2:19397/150‑1 (MQ=255)
                                                                                        caacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                         >  1:431607/1‑150 (MQ=255)
                                                                                        caacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                         >  1:983859/1‑150 (MQ=255)
                                                                                         aacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGga                                                        >  1:3938360/1‑150 (MQ=255)
                                                                                         aacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGga                                                        >  1:1738670/1‑150 (MQ=255)
                                                                                          acacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGgag                                                       >  1:2959896/1‑150 (MQ=255)
                                                                                           cacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGgaga                                                      >  2:3085702/1‑150 (MQ=255)
                                                                                           cacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGgaga                                                      >  2:3558730/1‑150 (MQ=255)
                                                                                           cacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGgaga                                                      >  2:3085579/1‑150 (MQ=255)
                                                                                            acGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAt                                                     >  1:914881/1‑150 (MQ=255)
                                                                                              gggTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATgg                                                   <  2:394108/150‑1 (MQ=255)
                                                                                               ggTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGt                                                  <  2:2641422/150‑1 (MQ=255)
                                                                                                  attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  1:3619308/150‑1 (MQ=255)
                                                                                                  attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  1:1703789/150‑1 (MQ=255)
                                                                                                  attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACACAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  2:1572875/150‑1 (MQ=255)
                                                                                                         tGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAg                                        <  2:1796168/150‑1 (MQ=255)
                                                                                                          gCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGc                                       >  2:84121/1‑150 (MQ=255)
                                                                                                               gtcgtcGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAg                                  >  1:500670/1‑150 (MQ=255)
                                                                                                                 cgtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGa                                >  2:3002055/1‑150 (MQ=255)
                                                                                                                     gAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAAt                            >  2:2692781/1‑150 (MQ=255)
                                                                                                                        tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCa                         >  2:1176248/1‑150 (MQ=255)
                                                                                                                        tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCa                         <  1:3823087/150‑1 (MQ=255)
                                                                                                                         aGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAc                        <  1:3054875/150‑1 (MQ=255)
                                                                                                                         aGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAc                        >  2:1002368/1‑150 (MQ=255)
                                                                                                                            gATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                     <  2:3127790/150‑1 (MQ=255)
                                                                                                                            gATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                     >  1:1952277/1‑150 (MQ=255)
                                                                                                                             aTGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAtt                    >  1:3254358/1‑150 (MQ=255)
                                                                                                                               gAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAtttt                  <  1:1071769/150‑1 (MQ=255)
                                                                                                                                 aTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcc                <  2:4104171/150‑1 (MQ=255)
                                                                                                                                 aTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcc                >  1:4114096/1‑150 (MQ=255)
                                                                                                                                  tAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCCAAAGGAAAATTCACCATTTTccc               >  1:2646696/1‑150 (MQ=255)
                                                                                                                                   aaGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc              >  2:4358349/1‑150 (MQ=255)
                                                                                                                                     gCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTg            >  1:2688504/1‑150 (MQ=255)
                                                                                                                                       tGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc          <  2:335527/150‑1 (MQ=255)
                                                                                                                                        gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCa         <  1:58404/150‑1 (MQ=255)
                                                                                                                                        gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCa         <  1:3649234/150‑1 (MQ=255)
                                                                                                                                        gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGACGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCa         >  2:933791/1‑150 (MQ=255)
                                                                                                                                          tCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACCGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACg       >  1:1612859/1‑150 (MQ=255)
                                                                                                                                            aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc     <  2:3950567/150‑1 (MQ=255)
                                                                                                                                            aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc     <  1:1787491/150‑1 (MQ=255)
                                                                                                                                            aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc     >  2:953920/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACG  >  CP009273/3111288‑3111580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACGGC  >  CP009273/3111285‑3111582
                                                                                                                                                     |                                                                                                                                                    
CCCAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTA                                                                                                                                                      >  SRR19973073.3410655/1‑150 (MQ=60)
 CCAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAG                                                                                                                                                     <  SRR19973073.1989982/150‑1 (MQ=60)
    GCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCC                                                                                                                                                  <  SRR19973073.3084254/150‑1 (MQ=60)
    GCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCC                                                                                                                                                  <  SRR19973073.3084256/150‑1 (MQ=60)
          CACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAA                                                                                                                                            <  SRR19973073.3926870/150‑1 (MQ=60)
          CACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAA                                                                                                                                            <  SRR19973073.3926634/150‑1 (MQ=60)
           ACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAAC                                                                                                                                           <  SRR19973073.1552940/150‑1 (MQ=60)
           ACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAAC                                                                                                                                           <  SRR19973073.1552946/150‑1 (MQ=60)
              GTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTC                                                                                                                                        >  SRR19973073.1489217/1‑150 (MQ=60)
               TTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCA                                                                                                                                       >  SRR19973073.3542306/1‑150 (MQ=60)
                TGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCAT                                                                                                                                      <  SRR19973073.1447697/150‑1 (MQ=60)
                TGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCAT                                                                                                                                      <  SRR19973073.1447673/150‑1 (MQ=60)
                   ATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAC                                                                                                                                   >  SRR19973073.2298622/1‑150 (MQ=60)
                    TAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACC                                                                                                                                  <  SRR19973073.278532/150‑1 (MQ=60)
                    TAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACC                                                                                                                                  <  SRR19973073.980320/150‑1 (MQ=60)
                    TAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACC                                                                                                                                  <  SRR19973073.980365/150‑1 (MQ=60)
                    TAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACC                                                                                                                                  <  SRR19973073.2545840/150‑1 (MQ=60)
                     AACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCC                                                                                                                                 >  SRR19973073.1115736/1‑150 (MQ=60)
                       CGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAG                                                                                                                               <  SRR19973073.4315994/150‑1 (MQ=60)
                       CGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAG                                                                                                                               <  SRR19973073.778882/150‑1 (MQ=60)
                             ATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGT                                                                                                                         >  SRR19973073.581927/1‑150 (MQ=60)
                             ATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGT                                                                                                                         >  SRR19973073.581936/1‑150 (MQ=60)
                               TGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCA                                                                                                                       <  SRR19973073.2535921/150‑1 (MQ=60)
                               TGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCA                                                                                                                       >  SRR19973073.4422271/1‑150 (MQ=60)
                                GATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAG                                                                                                                      >  SRR19973073.1729560/1‑150 (MQ=60)
                                GATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAG                                                                                                                      >  SRR19973073.1638845/1‑150 (MQ=60)
                                   AATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGC                                                                                                                   >  SRR19973073.2194728/1‑150 (MQ=60)
                                    ATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCA                                                                                                                  <  SRR19973073.421433/150‑1 (MQ=60)
                                       TCGTTGATCACGTTGGGATATTCGGCAAATACCTTGCGTACATAGTCCGGAACACCACGGGTATTATATTGCCCGGTCGTCGAATAGCGAAGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATG                                                                                                               >  SRR19973073.1055853/1‑150 (MQ=60)
                                         GTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGT                                                                                                             <  SRR19973073.1103544/150‑1 (MQ=60)
                                             ATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGAC                                                                                                         >  SRR19973073.4111970/1‑150 (MQ=60)
                                              TCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACT                                                                                                        <  SRR19973073.3055133/150‑1 (MQ=60)
                                               CACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTT                                                                                                       <  SRR19973073.2476730/150‑1 (MQ=60)
                                               CACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTT                                                                                                       <  SRR19973073.3801544/150‑1 (MQ=60)
                                                 CGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAT                                                                                                     >  SRR19973073.3730548/1‑150 (MQ=60)
                                                   TTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTG                                                                                                   <  SRR19973073.2688546/150‑1 (MQ=60)
                                                     GGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCC                                                                                                 <  SRR19973073.4591740/150‑1 (MQ=60)
                                                        ATATTCNGCAAAGACCTTGCGTACNTCGTCCGGNACAACACGGNTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCG                                                                                              <  SRR19973073.2663205/150‑1 (MQ=60)
                                                        ATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCG                                                                                              >  SRR19973073.3312155/1‑150 (MQ=60)
                                                        ATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCG                                                                                              <  SRR19973073.2911089/150‑1 (MQ=60)
                                                         TATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGC                                                                                             >  SRR19973073.4542301/1‑150 (MQ=60)
                                                          ATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCA                                                                                            >  SRR19973073.2836680/1‑150 (MQ=60)
                                                            TCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACT                                                                                          <  SRR19973073.2908888/150‑1 (MQ=60)
                                                             CGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTG                                                                                         <  SRR19973073.251558/150‑1 (MQ=60)
                                                              GGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGA                                                                                        <  SRR19973073.4272823/150‑1 (MQ=60)
                                                              GGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGA                                                                                        >  SRR19973073.3207073/1‑150 (MQ=60)
                                                              GGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGA                                                                                        <  SRR19973073.2002730/150‑1 (MQ=60)
                                                               GCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAA                                                                                       <  SRR19973073.2524535/150‑1 (MQ=60)
                                                                 AAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACC                                                                                     >  SRR19973073.652208/1‑150 (MQ=60)
                                                                 AAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACC                                                                                     >  SRR19973073.3355507/1‑150 (MQ=60)
                                                                 AAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACC                                                                                     >  SRR19973073.289183/1‑150 (MQ=60)
                                                                 AAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACC                                                                                     <  SRR19973073.621130/150‑1 (MQ=60)
                                                                  AAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCC                                                                                    <  SRR19973073.375077/150‑1 (MQ=60)
                                                                  AAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCC                                                                                    <  SRR19973073.375048/150‑1 (MQ=60)
                                                                     ACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGT                                                                                 >  SRR19973073.1012840/1‑150 (MQ=60)
                                                                     ACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGT                                                                                 >  SRR19973073.2269219/1‑150 (MQ=60)
                                                                      CCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTT                                                                                <  SRR19973073.1470370/150‑1 (MQ=60)
                                                                      CCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTT                                                                                <  SRR19973073.1470366/150‑1 (MQ=60)
                                                                       CTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTC                                                                               <  SRR19973073.3919129/150‑1 (MQ=60)
                                                                             TACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGT                                                                         <  SRR19973073.4355591/150‑1 (MQ=60)
                                                                               CATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAT                                                                       >  SRR19973073.4327605/1‑150 (MQ=60)
                                                                               CATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAT                                                                       >  SRR19973073.4325993/1‑150 (MQ=60)
                                                                               CATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAT                                                                       <  SRR19973073.4106142/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGTCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      >  SRR19973073.3418847/1‑150 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      <  SRR19973073.3598884/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      <  SRR19973073.3598871/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      <  SRR19973073.521194/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      <  SRR19973073.3171433/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      <  SRR19973073.3171453/150‑1 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      >  SRR19973073.2004163/1‑150 (MQ=60)
                                                                                ATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATC                                                                      >  SRR19973073.1363894/1‑150 (MQ=60)
                                                                                    TCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATA                                                                  <  SRR19973073.3118946/150‑1 (MQ=60)
                                                                                    TCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATA                                                                  <  SRR19973073.3118937/150‑1 (MQ=60)
                                                                                     CCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATAC                                                                 >  SRR19973073.2753831/1‑150 (MQ=60)
                                                                                     CCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATAC                                                                 <  SRR19973073.2090955/150‑1 (MQ=60)
                                                                                      CGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACC                                                                >  SRR19973073.4441996/1‑150 (MQ=60)
                                                                                       GGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCA                                                               <  SRR19973073.11553/150‑1 (MQ=60)
                                                                                        GAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAA                                                              >  SRR19973073.3108376/1‑150 (MQ=60)
                                                                                           CAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGG                                                           >  SRR19973073.985912/1‑150 (MQ=60)
                                                                                           CAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGG                                                           >  SRR19973073.803515/1‑150 (MQ=60)
                                                                                           CAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGG                                                           >  SRR19973073.432476/1‑150 (MQ=60)
                                                                                            AACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGA                                                          >  SRR19973073.1742281/1‑150 (MQ=60)
                                                                                            AACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGA                                                          >  SRR19973073.3946206/1‑150 (MQ=60)
                                                                                            AACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGA                                                          >  SRR19973073.1742193/1‑150 (MQ=60)
                                                                                             ACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAG                                                         >  SRR19973073.2965927/1‑150 (MQ=60)
                                                                                             ACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAG                                                         <  SRR19973073.1157328/150‑1 (MQ=60)
                                                                                              CACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGA                                                        <  SRR19973073.52601/150‑1 (MQ=60)
                                                                                               ACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAT                                                       >  SRR19973073.916779/1‑150 (MQ=60)
                                                                                               ACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAT                                                       <  SRR19973073.2145586/150‑1 (MQ=60)
                                                                                                CGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATG                                                      <  SRR19973073.4394892/150‑1 (MQ=60)
                                                                                                CGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATG                                                      <  SRR19973073.283622/150‑1 (MQ=60)
                                                                                                     ATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTC                                                 <  SRR19973073.1707325/150‑1 (MQ=60)
                                                                                                     ATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTC                                                 <  SRR19973073.3626577/150‑1 (MQ=60)
                                                                                                               CCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTA                                       <  SRR19973073.3126544/150‑1 (MQ=60)
                                                                                                               CCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTA                                       >  SRR19973073.3539324/1‑150 (MQ=60)
                                                                                                                 GGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAA                                     >  SRR19973073.2841256/1‑150 (MQ=60)
                                                                                                                  GTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAG                                    >  SRR19973073.501809/1‑150 (MQ=60)
                                                                                                                  GTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAG                                    >  SRR19973073.501804/1‑150 (MQ=60)
                                                                                                                  GTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAG                                    >  SRR19973073.501744/1‑150 (MQ=60)
                                                                                                                   TCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGG                                   <  SRR19973073.986310/150‑1 (MQ=60)
                                                                                                                   TCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGG                                   <  SRR19973073.3912249/150‑1 (MQ=60)
                                                                                                                      TCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAA                                <  SRR19973073.4266785/150‑1 (MQ=60)
                                                                                                                       CGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAA                               >  SRR19973073.918643/1‑150 (MQ=60)
                                                                                                                       CGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAA                               >  SRR19973073.918644/1‑150 (MQ=60)
                                                                                                                        GAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAAT                              <  SRR19973073.1742398/150‑1 (MQ=60)
                                                                                                                        GAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAAT                              >  SRR19973073.2440509/1‑150 (MQ=60)
                                                                                                                           TAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCA                           <  SRR19973073.3261445/150‑1 (MQ=60)
                                                                                                                           TAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCA                           <  SRR19973073.3830696/150‑1 (MQ=60)
                                                                                                                            AGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAC                          <  SRR19973073.3061036/150‑1 (MQ=60)
                                                                                                                               GATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT                       >  SRR19973073.4076723/1‑150 (MQ=60)
                                                                                                                               GATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT                       <  SRR19973073.1545990/150‑1 (MQ=60)
                                                                                                                               GATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAT                       >  SRR19973073.1956293/1‑150 (MQ=60)
                                                                                                                                ATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATT                      >  SRR19973073.3260940/1‑150 (MQ=60)
                                                                                                                                  GAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTT                    <  SRR19973073.1073983/150‑1 (MQ=60)
                                                                                                                                  GAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTT                    <  SRR19973073.3635508/150‑1 (MQ=60)
                                                                                                                                    ATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCC                  >  SRR19973073.4122329/1‑150 (MQ=60)
                                                                                                                                     TAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCCAAAGGAAAATTCACCATTTTCCC                 >  SRR19973073.2652125/1‑150 (MQ=60)
                                                                                                                                        GCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTG              >  SRR19973073.2693999/1‑150 (MQ=60)
                                                                                                                                           GATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCA           <  SRR19973073.58567/150‑1 (MQ=60)
                                                                                                                                           GATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCA           <  SRR19973073.3656556/150‑1 (MQ=60)
                                                                                                                                             TCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACCGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACG         >  SRR19973073.1616185/1‑150 (MQ=60)
                                                                                                                                               AATATTATCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCC       <  SRR19973073.1791381/150‑1 (MQ=60)
                                                                                                                                               AATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCC       <  SRR19973073.1791184/150‑1 (MQ=60)
                                                                                                                                                    TAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACGGC  >  SRR19973073.2282373/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CCCAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACGGC  >  CP009273/3111285‑3111582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑