Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,298,059 C→A 17.1% R373L (CGC→CTC)  mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,298,0590CA17.1% 879.5 / 110.9 283R373L (CGC→CTC) mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (137/97);  new base A (28/21);  total (165/118)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTC  >  CP009273/3297910‑3298208
                                                                                                                                                     |                                                                                                                                                     
aTGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGc                                                                                                                                                       >  2:3827333/1‑150 (MQ=255)
    cGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGttt                                                                                                                                                   >  2:2762292/1‑150 (MQ=255)
      gATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTAc                                                                                                                                                 <  1:27628/150‑1 (MQ=255)
        tAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTTCCGACGCCAAACACCAGAATCAGCGCACTCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGGTTAcgc                                                                                                                                               >  1:2383021/1‑150 (MQ=255)
        tAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTAcgc                                                                                                                                               >  1:2383223/1‑150 (MQ=255)
        aaaGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTTCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTAcgc                                                                                                                                               >  2:1730275/2‑150 (MQ=255)
         aaGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTAcgcg                                                                                                                                              >  2:3848665/1‑150 (MQ=255)
         aaGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTAcgcg                                                                                                                                              >  2:4623274/1‑150 (MQ=255)
          aGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGa                                                                                                                                             >  2:2429952/1‑150 (MQ=255)
           ggCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGAt                                                                                                                                            <  2:3711588/150‑1 (MQ=255)
           ggCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGAt                                                                                                                                            <  2:2577878/150‑1 (MQ=255)
            gCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATg                                                                                                                                           <  1:3502505/150‑1 (MQ=255)
              aCCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCa                                                                                                                                         <  1:3559783/150‑1 (MQ=255)
              aCCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCa                                                                                                                                         >  1:4372986/1‑150 (MQ=255)
                       tACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAAc                                                                                                                                >  2:4210762/1‑150 (MQ=255)
                        aCTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACa                                                                                                                               >  1:3058945/1‑150 (MQ=255)
                          tGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAgc                                                                                                                             <  2:2815246/150‑1 (MQ=255)
                           gATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAgcg                                                                                                                            <  1:3308177/150‑1 (MQ=255)
                              acacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCg                                                                                                                         >  1:3903647/1‑150 (MQ=255)
                              acacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCg                                                                                                                         >  1:3814190/1‑150 (MQ=255)
                               cacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCgg                                                                                                                        >  1:4355004/1‑150 (MQ=255)
                               cacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCgg                                                                                                                        <  2:4572687/150‑1 (MQ=255)
                               cacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCgg                                                                                                                        >  1:1639051/1‑150 (MQ=255)
                                   ggCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACa                                                                                                                    <  1:2707686/150‑1 (MQ=255)
                                      aGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAAtt                                                                                                                 <  1:3944693/150‑1 (MQ=255)
                                      aGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAAtt                                                                                                                 <  2:3474988/150‑1 (MQ=255)
                                       gTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTg                                                                                                                <  2:117580/150‑1 (MQ=255)
                                        tAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTgc                                                                                                               <  1:2952881/150‑1 (MQ=255)
                                            ttAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTgccgcc                                                                                                           <  2:340543/150‑1 (MQ=255)
                                              aaaGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCACAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCa                                                                                                         >  2:3085144/1‑150 (MQ=255)
                                               aaGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAg                                                                                                        >  2:672000/1‑150 (MQ=255)
                                               aaGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAg                                                                                                        <  1:1194182/150‑1 (MQ=255)
                                               aaGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAg                                                                                                        >  2:2075797/1‑150 (MQ=255)
                                               aaGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAg                                                                                                        >  2:3178038/1‑150 (MQ=255)
                                                aGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGa                                                                                                       >  1:3131908/1‑150 (MQ=255)
                                                  cTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATg                                                                                                     <  2:1405831/150‑1 (MQ=255)
                                                   tCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATgg                                                                                                    >  1:230890/1‑150 (MQ=255)
                                                   tCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATgg                                                                                                    >  1:1004519/1‑150 (MQ=255)
                                                    cGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGACTCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGt                                                                                                   <  2:525612/150‑1 (MQ=255)
                                                     gATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTa                                                                                                  >  1:3789809/1‑150 (MQ=255)
                                                       tAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGc                                                                                                >  1:4166635/1‑150 (MQ=255)
                                                        aaaaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGcc                                                                                               <  1:3707691/150‑1 (MQ=255)
                                                        aaaaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGcc                                                                                               <  2:3641746/150‑1 (MQ=255)
                                                        aaaaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGcc                                                                                               >  2:1133205/1‑150 (MQ=255)
                                                         aaaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCg                                                                                              >  1:2113447/1‑150 (MQ=255)
                                                         aaaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCg                                                                                              >  1:3965305/1‑150 (MQ=255)
                                                            tatGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTa                                                                                           >  1:1716111/1‑150 (MQ=255)
                                                                cACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAAcc                                                                                       <  2:2284807/150‑1 (MQ=255)
                                                                 aCCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCa                                                                                      >  1:1578623/1‑150 (MQ=255)
                                                                      tGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATa                                                                                 >  1:2579986/1‑150 (MQ=255)
                                                                       gCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATaa                                                                                >  1:1042242/1‑150 (MQ=255)
                                                                         gTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                              >  2:4337059/1‑150 (MQ=255)
                                                                         gTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                              >  2:1878172/1‑150 (MQ=255)
                                                                         gTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                              >  2:44829/1‑150 (MQ=255)
                                                                         gTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                              >  2:3056440/1‑150 (MQ=255)
                                                                         gTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCAACCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                              >  1:3349428/1‑150 (MQ=255)
                                                                           tGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCaa                                                                            <  1:533940/150‑1 (MQ=255)
                                                                               gACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGc                                                                        >  2:444974/1‑150 (MQ=255)
                                                                                aCGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCg                                                                       <  2:3216710/150‑1 (MQ=255)
                                                                                  gCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTa                                                                     >  2:2885131/1‑150 (MQ=255)
                                                                                  gCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATTGCGTa                                                                     <  2:1433487/150‑1 (MQ=255)
                                                                                  gCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATTGCGTa                                                                     <  2:3110095/150‑1 (MQ=255)
                                                                                    cAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACa                                                                   >  1:1771531/1‑150 (MQ=255)
                                                                                    cAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACa                                                                   >  2:1830966/1‑150 (MQ=255)
                                                                                      aaCACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAgg                                                                 <  2:821200/150‑1 (MQ=255)
                                                                                      aaCACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAgg                                                                 <  2:3239346/150‑1 (MQ=255)
                                                                                      aaCACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAgg                                                                 >  1:617357/1‑150 (MQ=255)
                                                                                         acCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAAt                                                              >  1:920618/1‑150 (MQ=255)
                                                                                           cAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATcc                                                            <  2:2269646/150‑1 (MQ=255)
                                                                                             gAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGt                                                          <  2:4169246/150‑1 (MQ=255)
                                                                                             gAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGt                                                          >  2:3531984/1‑150 (MQ=255)
                                                                                                  aGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCggg                                                     >  1:1491158/1‑150 (MQ=255)
                                                                                                  aGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCggg                                                     <  1:3613299/150‑1 (MQ=255)
                                                                                                  aGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCggg                                                     <  1:1963432/150‑1 (MQ=255)
                                                                                                  aGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCggg                                                     <  1:1963423/150‑1 (MQ=255)
                                                                                                     gcAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGaac                                                  <  1:4244027/150‑1 (MQ=255)
                                                                                                         tcatcGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAacaaca                                              <  2:1736014/150‑1 (MQ=255)
                                                                                                         tcatcGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAacaaca                                              >  2:3971966/1‑150 (MQ=255)
                                                                                                              ggCTTGCCACCCCAGACGCGGACTTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                         >  2:750325/1‑150 (MQ=255)
                                                                                                               gCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGAAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                        >  1:1234945/1‑150 (MQ=255)
                                                                                                                 ttGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccc                                      >  1:3651953/1‑150 (MQ=255)
                                                                                                                  tGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGTCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCca                                     <  1:1437755/150‑1 (MQ=255)
                                                                                                                       ccccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACt                                >  2:1527676/1‑150 (MQ=255)
                                                                                                                        cccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTg                               <  2:4271842/150‑1 (MQ=255)
                                                                                                                        cccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTg                               <  2:4008847/150‑1 (MQ=255)
                                                                                                                         ccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTgg                              <  2:3568978/150‑1 (MQ=255)
                                                                                                                           aGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCg                            >  1:187125/1‑150 (MQ=255)
                                                                                                                           aGACGCGGAATTTCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCg                            <  1:1314851/150‑1 (MQ=255)
                                                                                                                            gACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCgg                           >  2:4376029/1‑150 (MQ=255)
                                                                                                                             aCGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACACCTGGCggg                          <  1:2504840/150‑1 (MQ=255)
                                                                                                                                cgGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCa                       >  2:2824313/1‑150 (MQ=255)
                                                                                                                                 ggAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCaa                      >  1:3197671/1‑150 (MQ=255)
                                                                                                                                   aaTTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAg                    >  1:975978/1‑150 (MQ=255)
                                                                                                                                   aaTTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAg                    >  1:3546618/1‑150 (MQ=255)
                                                                                                                                     tttCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGt                  <  1:3804798/150‑1 (MQ=255)
                                                                                                                                       tCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCa                >  1:2914069/1‑150 (MQ=255)
                                                                                                                                       tCGGGCTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCa                <  1:1134993/150‑1 (MQ=255)
                                                                                                                                         gggCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGc              >  2:2133783/1‑150 (MQ=255)
                                                                                                                                                 aaaGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGt      >  2:909989/1‑150 (MQ=255)
                                                                                                                                                     cGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTc  <  1:732074/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTC  >  CP009273/3297910‑3298208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A