Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,752,893 T→A 27.2% P30P (CCA→CCT)  ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,752,8930TA27.2% 541.3 / 228.5 306P30P (CCA→CCT) ldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (118/104);  new base A (44/39);  total (162/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCA  >  CP009273/1752744‑1753042
                                                                                                                                                     |                                                                                                                                                     
   acAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGTGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCTTATACGTTGGATTTTGCACAACCAGTCGGCGTCCGGTtggt                                                                                                                                                    >  2:2540666/2‑150 (MQ=255)
    cAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTTCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATACCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTtggtg                                                                                                                                                   >  1:1711920/1‑150 (MQ=255)
    cAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTtggtg                                                                                                                                                   <  1:1603888/150‑1 (MQ=255)
     aGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTtggtgg                                                                                                                                                  >  2:242102/1‑150 (MQ=255)
      ggTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTggtggt                                                                                                                                                 >  1:2968969/1‑150 (MQ=255)
      ggTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAggtggt                                                                                                                                                 >  2:3459468/1‑150 (MQ=255)
            ggCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGa                                                                                                                                           >  1:3708705/1‑150 (MQ=255)
             gCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGAt                                                                                                                                          <  2:3240776/150‑1 (MQ=255)
             gCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGAt                                                                                                                                          >  1:1409943/1‑150 (MQ=255)
              cACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATa                                                                                                                                         >  1:181914/1‑150 (MQ=255)
               aCCGGGGCGAGAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATaa                                                                                                                                        <  2:535848/150‑1 (MQ=255)
                    ggCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAAc                                                                                                                                   >  2:1773192/1‑150 (MQ=255)
                     gCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAAcc                                                                                                                                  <  2:1204043/150‑1 (MQ=255)
                      cGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCg                                                                                                                                 >  2:3130695/1‑150 (MQ=255)
                         tAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGccc                                                                                                                              >  2:2276372/1‑150 (MQ=255)
                           gtgtTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAg                                                                                                                            >  2:4434077/1‑150 (MQ=255)
                            tgtTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAgg                                                                                                                           >  1:380095/1‑150 (MQ=255)
                             gtTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGc                                                                                                                          >  2:4118314/1‑150 (MQ=255)
                               tATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAg                                                                                                                        >  1:514028/1‑150 (MQ=255)
                               tATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGGTGGTGGTAGCGGATAATCAACCGCCCAGGCAg                                                                                                                        >  2:2614395/1‑150 (MQ=255)
                                aTTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGc                                                                                                                       <  1:2056737/150‑1 (MQ=255)
                                  tGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCt                                                                                                                     <  2:2975400/150‑1 (MQ=255)
                                      ttCAAGGATCAACATTGCCGCAGTATCAAAACGTAGGGAAATAGCCTGAATTTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGTTAGCGGATAATCAACCGCCCAGGCAGCCTGGAt                                                                                                                 <  1:2428900/150‑1 (MQ=255)
                                         aaGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGgc                                                                                                              <  1:726310/150‑1 (MQ=255)
                                         aaGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGgc                                                                                                              <  1:2597372/150‑1 (MQ=255)
                                          aGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGGTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGgcg                                                                                                             >  2:1603670/1‑150 (MQ=255)
                                           ggATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGgcgc                                                                                                            >  1:4466743/1‑150 (MQ=255)
                                            gATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATACTCAACCGCCCAGGCAGCCTGGATGGCGCt                                                                                                           >  1:4092823/1‑150 (MQ=255)
                                            gATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCt                                                                                                           <  1:2347110/150‑1 (MQ=255)
                                             aTCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTa                                                                                                          <  1:2711709/150‑1 (MQ=255)
                                             aTCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTa                                                                                                          <  2:2295055/150‑1 (MQ=255)
                                               cAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTaaa                                                                                                        >  2:3448882/1‑150 (MQ=255)
                                               cAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTaaa                                                                                                        <  1:883364/150‑1 (MQ=255)
                                                aaCATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTaaaa                                                                                                       >  2:282663/1‑150 (MQ=255)
                                                aaCATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTaaaa                                                                                                       >  2:1031022/1‑150 (MQ=255)
                                                 aCATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAg                                                                                                      <  2:3486963/150‑1 (MQ=255)
                                                  cATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAgc                                                                                                     <  1:53474/150‑1 (MQ=255)
                                                  cATTGCCGCAGTATAAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAgc                                                                                                     >  1:3015151/1‑150 (MQ=255)
                                                     tGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGcc                                                                                                  <  1:4652347/150‑1 (MQ=255)
                                                          cAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATaa                                                                                             >  2:823465/1‑150 (MQ=255)
                                                           tgTATCAAAACGTCGGGAAATAGCCTTATGTTTTTTATCCCCTTCTTGCACCTTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGTTTGGTGGTAGCGTTTAATCAACCGCCCAGGCAGCCTGGTTGGCGATAAAAGCGCCGATAAg                                                                                            <  1:4188600/149‑1 (MQ=255)
                                                           aGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAg                                                                                            >  2:2214658/1‑150 (MQ=255)
                                                               taaaaaCTTCGGTCAATAGCCTTAAGTTTTTTATCCCATTCTTGAACAGTATACGTTTTATTTTGCCAAACAAGTCGGCTTCCGGTAGGTGTTAGCGTATAATCAACCGCCCCGGCAGCCTGGTTGGCGCTAAAAGCGCCGATAAGCGTg                                                                                        <  1:3885094/148‑1 (MQ=255)
                                                                   aaCGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAgtg                                                                                    >  1:265883/1‑150 (MQ=255)
                                                                   aaCGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAgtg                                                                                    <  2:129824/150‑1 (MQ=255)
                                                                    aCGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAgtgt                                                                                   <  2:204814/150‑1 (MQ=255)
                                                                     cGTCGGGCAATTGCCTGATGGTTTTTATCCCCTTCTTGCACCGTTTACGTTTGATTTTGCCCAACCCGTCGGCTTCCGGTTGGTGGTAGCGGATTATCTACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATACGCGTGAGTGTa                                                                                  <  2:4701080/150‑1 (MQ=255)
                                                                     cGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTa                                                                                  >  1:2857403/1‑150 (MQ=255)
                                                                       tCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAg                                                                                >  1:3115883/1‑150 (MQ=255)
                                                                             aaTAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGa                                                                          >  1:2695276/1‑150 (MQ=255)
                                                                              aTAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTTTAGGTTTGATTTTGCCCAACCAGTCGGCTTCGGGTTGGTGGTTGCGTATAATCAACCGCCCAGGCAGCCTGGTTGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAc                                                                         <  1:3916617/150‑1 (MQ=255)
                                                                                aGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgc                                                                       >  2:1989074/1‑150 (MQ=255)
                                                                                  ccTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcgc                                                                     >  1:2121182/1‑150 (MQ=255)
                                                                                   cTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcgcg                                                                    >  1:2102126/1‑150 (MQ=255)
                                                                                   cTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcgcg                                                                    >  1:1554993/1‑150 (MQ=255)
                                                                                   cTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcgcg                                                                    >  1:3250405/1‑150 (MQ=255)
                                                                                      taGGTTTTTATCCCCTTGTTTCACAGTTTACGTTTTTTTTTGCCAAACCTGTGGGCTTCGGTTTGGTGGTTGGGTATAATAAACCGCCCAGGCTTCCGTGATGGCGATAAACGCGCCGATAAGCGTGAGTGTAAGCAAAGACTCGAGttt                                                                 <  2:4405952/149‑1 (MQ=255)
                                                                                      aaGGTTTTTATCCCCTTCGTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGttt                                                                 <  2:4294749/150‑1 (MQ=255)
                                                                                        ggTTTTTCTCCCCTCCTTGCACCGTATACGTTTGATTTTGCCCAACCAGGCGTCTTCCGGTAGGTGGTAGCGGATAATCAACCCCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCa                                                               <  2:265325/150‑1 (MQ=255)
                                                                                        ggTTTTTATCCCCTTCTTGCACCGTATACGTTTTATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCa                                                               <  1:4339480/150‑1 (MQ=255)
                                                                                          tttttATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCAtt                                                             <  2:1132205/150‑1 (MQ=255)
                                                                                           ttttATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGTCAGCCTGGTTGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTg                                                            <  1:992655/150‑1 (MQ=255)
                                                                                            tttATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAATGCGCCGATAACCGTGATTTTAAGAAAAGACGCGCGTTTCATTGt                                                           >  2:933787/1‑150 (MQ=255)
                                                                                            tttATCCCCTTCGTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGt                                                           <  1:3073092/150‑1 (MQ=255)
                                                                                               aTCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTaaa                                                        >  1:775466/1‑150 (MQ=255)
                                                                                                  cccTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcct                                                     <  2:3171853/150‑1 (MQ=255)
                                                                                                   ccTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctc                                                    >  2:1907516/1‑150 (MQ=255)
                                                                                                      tcttGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGTATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTg                                                 <  2:4493979/150‑1 (MQ=255)
                                                                                                       cttGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGt                                                <  2:3708705/150‑1 (MQ=255)
                                                                                                       cttGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGt                                                >  1:2751720/1‑150 (MQ=255)
                                                                                                           cACCTTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGTATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTAttt                                            <  1:3271735/150‑1 (MQ=255)
                                                                                                           cACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTAttt                                            >  1:131221/1‑150 (MQ=255)
                                                                                                           cACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTAttt                                            <  1:739369/150‑1 (MQ=255)
                                                                                                            aCCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTg                                           <  2:2714952/150‑1 (MQ=255)
                                                                                                            aCCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTg                                           <  2:3537768/150‑1 (MQ=255)
                                                                                                             ccGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGc                                          >  2:2070308/1‑150 (MQ=255)
                                                                                                              cGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGcc                                         <  2:136329/150‑1 (MQ=255)
                                                                                                              cGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGATAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGcc                                         <  1:2166339/150‑1 (MQ=255)
                                                                                                                 ataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGga                                      <  2:3831035/150‑1 (MQ=255)
                                                                                                                  taCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGgag                                     <  2:728247/150‑1 (MQ=255)
                                                                                                                    cGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGAc                                   <  1:124901/150‑1 (MQ=255)
                                                                                                                          aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGc                             >  2:1598994/1‑150 (MQ=255)
                                                                                                                          aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGc                             >  1:58978/1‑150 (MQ=255)
                                                                                                                           ttttGCCCAACCAGTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCt                            >  2:2762999/1‑150 (MQ=255)
                                                                                                                            tttGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTg                           <  2:3557706/150‑1 (MQ=255)
                                                                                                                            tttGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTg                           <  1:1156750/150‑1 (MQ=255)
                                                                                                                               gCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGaaa                        >  1:4736000/1‑150 (MQ=255)
                                                                                                                                cccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAAc                       <  1:355527/150‑1 (MQ=255)
                                                                                                                                 ccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACg                      >  2:4188864/1‑150 (MQ=255)
                                                                                                                                    aCCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCg                   >  2:593721/1‑150 (MQ=255)
                                                                                                                                     ccAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCgg                  <  2:379729/150‑1 (MQ=255)
                                                                                                                                      cAGTGGGCTTCGGGTTGGTGGTCGCGTATAATCACCCGCCCAGGCAGCCTGGAGGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGa                 <  1:823423/150‑1 (MQ=255)
                                                                                                                                       aGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGAt                >  1:1537978/1‑150 (MQ=255)
                                                                                                                                        gTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATg               <  2:2120673/150‑1 (MQ=255)
                                                                                                                                        gTCGGCTTCCGGTAGGTGGTAGCGGATAATCAACCGCCCAGGCAGTCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATg               >  2:1136197/1‑150 (MQ=255)
                                                                                                                                         tCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGTATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATgg              <  1:3457939/150‑1 (MQ=255)
                                                                                                                                             cTTCCGGTTGGTGGTAGCGTATAATCAACCGCCCAGGCAGCCTGTATGGCGATAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCt          <  1:2035038/150‑1 (MQ=255)
                                                                                                                                               tCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTa        >  1:8687/1‑150 (MQ=255)
                                                                                                                                               tCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTa        >  1:1011395/1‑150 (MQ=255)
                                                                                                                                                   gTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGtt    <  2:2880306/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCA  >  CP009273/1752744‑1753042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A