Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,442,790 C→A 24.5% D247Y (GAT→TAT)  yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,442,7900CA24.5% 713.4 / 192.6 300D247Y (GAT→TAT) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (92/134);  new base A (26/48);  total (118/182)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

TGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCC  >  CP009273/2442642‑2442939
                                                                                                                                                    |                                                                                                                                                     
 gAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgt                                                                                                                                                     <  1:175362/150‑1 (MQ=255)
        ttGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTg                                                                                                                                              >  2:3582522/1‑150 (MQ=255)
          gcgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGtt                                                                                                                                            <  1:2067493/150‑1 (MQ=255)
            gcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTg                                                                                                                                          <  1:4030895/150‑1 (MQ=255)
            gcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTg                                                                                                                                          >  2:2504380/1‑150 (MQ=255)
                 gTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGt                                                                                                                                     >  2:2473030/1‑150 (MQ=255)
                 gTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGt                                                                                                                                     <  2:3189305/150‑1 (MQ=255)
                  tACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGtt                                                                                                                                    <  1:1557630/150‑1 (MQ=255)
                   aCGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTta                                                                                                                                   <  2:1030739/150‑1 (MQ=255)
                     gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                                                                                                                                 >  2:2191584/1‑150 (MQ=255)
                       gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATCTCGTGTAACTGTTTGTCCGTTATAgg                                                                                                                               <  2:1706169/150‑1 (MQ=255)
                        tAAGGGCAGGGGCGAATGCCCCTGCCGGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAggg                                                                                                                              <  1:3295327/150‑1 (MQ=255)
                         aaTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGgt                                                                                                                             >  1:3037597/1‑150 (MQ=255)
                            ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTc                                                                                                                          <  2:837552/150‑1 (MQ=255)
                              cAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGa                                                                                                                        <  1:1622172/150‑1 (MQ=255)
                                ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                                                                                                                      <  2:3985846/150‑1 (MQ=255)
                                   gCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTgg                                                                                                                   <  2:1461401/150‑1 (MQ=255)
                                    cGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGc                                                                                                                  <  1:3883131/150‑1 (MQ=255)
                                       aTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTg                                                                                                               >  1:864359/1‑150 (MQ=255)
                                           cccTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg                                                                                                           >  1:4110448/1‑150 (MQ=255)
                                             ctgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCgag                                                                                                         >  2:2261668/1‑150 (MQ=255)
                                              tgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCgaga                                                                                                        >  2:4154960/1‑150 (MQ=255)
                                                cctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACt                                                                                                      >  2:1616990/1‑150 (MQ=255)
                                                    gtgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCg                                                                                                  >  1:4566875/1‑150 (MQ=255)
                                                     tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGc                                                                                                 >  1:2542571/1‑150 (MQ=255)
                                                      gtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGcc                                                                                                >  2:4404912/1‑150 (MQ=255)
                                                       tATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCt                                                                                               <  2:3928000/150‑1 (MQ=255)
                                                        aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                                              >  1:439021/1‑150 (MQ=255)
                                                        aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                                              >  1:3700146/1‑150 (MQ=255)
                                                        aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                                              <  2:2792619/150‑1 (MQ=255)
                                                        aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                                              >  1:109375/1‑150 (MQ=255)
                                                         tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTg                                                                                             <  2:4747478/150‑1 (MQ=255)
                                                         tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTg                                                                                             <  1:4159636/150‑1 (MQ=255)
                                                          cTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGt                                                                                            <  2:3842915/150‑1 (MQ=255)
                                                            aaGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTca                                                                                          >  2:4659630/1‑150 (MQ=255)
                                                              gggCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTcaca                                                                                        >  1:2018858/1‑150 (MQ=255)
                                                               ggCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACAt                                                                                       <  2:1798806/150‑1 (MQ=255)
                                                               ggCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACAt                                                                                       >  1:683584/1‑150 (MQ=255)
                                                                 cTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATcc                                                                                     <  1:2191584/150‑1 (MQ=255)
                                                                   aCAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTTCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCg                                                                                   <  2:711266/150‑1 (MQ=255)
                                                                     aaCTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAc                                                                                 >  2:345350/1‑150 (MQ=255)
                                                                      aCTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAcg                                                                                <  2:425773/150‑1 (MQ=255)
                                                                       cTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAcgc                                                                               >  1:2959156/1‑150 (MQ=255)
                                                                         cATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCgg                                                                             >  1:1060316/1‑150 (MQ=255)
                                                                         cATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCgg                                                                             <  1:198831/150‑1 (MQ=255)
                                                                          aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                                            <  1:4445638/150‑1 (MQ=255)
                                                                           ttCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTa                                                                           <  1:350587/150‑1 (MQ=255)
                                                                           ttCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTa                                                                           <  1:499031/150‑1 (MQ=255)
                                                                                   ggaCTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTat                                                                   <  1:2666043/148‑1 (MQ=255)
                                                                                     aCTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatca                                                                 <  2:3398883/150‑1 (MQ=255)
                                                                                       tGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatcatc                                                               >  1:3380363/1‑150 (MQ=255)
                                                                                         gAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtg                                                             <  2:3152449/150‑1 (MQ=255)
                                                                                          aaCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtgt                                                            >  2:4484940/1‑150 (MQ=255)
                                                                                           aCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtgtg                                                           <  2:645987/150‑1 (MQ=255)
                                                                                            ccGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGc                                                          <  2:3634216/150‑1 (MQ=255)
                                                                                            ccGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGc                                                          <  2:413761/150‑1 (MQ=255)
                                                                                             cGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCa                                                         <  1:3214374/150‑1 (MQ=255)
                                                                                                 aaGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAAccc                                                     <  1:1731335/150‑1 (MQ=255)
                                                                                                  aGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCa                                                    <  1:1520387/150‑1 (MQ=255)
                                                                                                   gTAGACTCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAg                                                   <  2:4292479/150‑1 (MQ=255)
                                                                                                       aaTCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGc                                               >  1:1795254/1‑150 (MQ=255)
                                                                                                        aTCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCa                                              <  2:554479/150‑1 (MQ=255)
                                                                                                           gATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTa                                           >  2:4634487/1‑150 (MQ=255)
                                                                                                           gATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTa                                           <  1:4322079/150‑1 (MQ=255)
                                                                                                           gATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTa                                           <  1:464214/150‑1 (MQ=255)
                                                                                                            aTTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTaa                                          >  1:831405/1‑150 (MQ=255)
                                                                                                              tGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacc                                        <  1:4748366/150‑1 (MQ=255)
                                                                                                              tGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacc                                        <  1:1996999/150‑1 (MQ=255)
                                                                                                               ggCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacca                                       >  1:2815587/1‑150 (MQ=255)
                                                                                                                gCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAaccac                                      >  1:1448789/1‑150 (MQ=255)
                                                                                                                 cTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAaccacc                                     <  1:278744/150‑1 (MQ=255)
                                                                                                                  tGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt                                    <  2:2948257/150‑1 (MQ=255)
                                                                                                                  tGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt                                    <  2:207945/150‑1 (MQ=255)
                                                                                                                    cATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGt                                  <  1:1436966/150‑1 (MQ=255)
                                                                                                                    cATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGt                                  <  1:4405301/150‑1 (MQ=255)
                                                                                                                     aTACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCCACCCAGCGGCAGTAACCACCTGtt                                 >  1:4476267/1‑150 (MQ=255)
                                                                                                                      tACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGttt                                <  1:2976405/150‑1 (MQ=255)
                                                                                                                      tACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGttt                                <  2:2001010/150‑1 (MQ=255)
                                                                                                                       aCTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttc                               >  1:4720506/1‑150 (MQ=255)
                                                                                                                       aCTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttc                               <  1:3804787/150‑1 (MQ=255)
                                                                                                                          gctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcatt                            <  2:2121004/150‑1 (MQ=255)
                                                                                                                              gTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATc                        <  2:445385/150‑1 (MQ=255)
                                                                                                                               tctCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCt                       <  1:2261668/148‑1 (MQ=255)
                                                                                                                                ctCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTg                      <  2:1743059/149‑1 (MQ=255)
                                                                                                                                 tCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGc                     >  1:3147965/1‑150 (MQ=255)
                                                                                                                                 tCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGc                     <  2:251059/150‑1 (MQ=255)
                                                                                                                                   aaCACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGt                   >  1:2355263/1‑150 (MQ=255)
                                                                                                                                        gcacgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGt              <  2:109375/150‑1 (MQ=255)
                                                                                                                                         cacgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGTTTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTc             >  1:126319/1‑150 (MQ=255)
                                                                                                                                         cacgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTc             <  2:128948/150‑1 (MQ=255)
                                                                                                                                          acgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCg            >  1:1926942/1‑150 (MQ=255)
                                                                                                                                          acgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCg            <  2:2355263/150‑1 (MQ=255)
                                                                                                                                          acgACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCg            <  2:585759/150‑1 (MQ=255)
                                                                                                                                            gACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGca          <  2:1192321/150‑1 (MQ=255)
                                                                                                                                            gACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGca          >  2:462161/1‑150 (MQ=255)
                                                                                                                                              ccATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGcaca        <  1:2504141/150‑1 (MQ=255)
                                                                                                                                              ccATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGcaca        >  2:2233974/1‑150 (MQ=255)
                                                                                                                                              ccATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGcaca        >  2:733357/1‑150 (MQ=255)
                                                                                                                                                    cGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTcc  >  1:4486145/1‑150 (MQ=255)
                                                                                                                                                    cGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTcc  >  1:1844017/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCC  >  CP009273/2442642‑2442939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A