Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,993,530 C→A 100% E113* (GAG→TAG)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,993,5300CA99.0% 618.8 / ‑3.3 189E113* (GAG→TAG) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (87/100);  total (88/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAA  >  CP009273/1993383‑1993672
                                                                                                                                                   |                                                                                                                                              
       gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaa                                                                                                                                         <  2:3439086/148‑1 (MQ=255)
        ttGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaaa                                                                                                                                        <  1:1636321/148‑1 (MQ=255)
           gTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATgg                                                                                                                                     <  1:2202806/148‑1 (MQ=255)
            tGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGt                                                                                                                                    <  1:2844792/148‑1 (MQ=255)
            tGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGt                                                                                                                                    <  1:2487426/148‑1 (MQ=255)
               ccAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAc                                                                                                                                 <  2:1162983/148‑1 (MQ=255)
                cAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAcc                                                                                                                                <  1:3463805/148‑1 (MQ=255)
                cAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAcc                                                                                                                                <  2:1863808/148‑1 (MQ=255)
                  gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                              <  1:1669474/148‑1 (MQ=255)
                  gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                              <  2:1563690/148‑1 (MQ=255)
                  gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                              <  1:1957503/148‑1 (MQ=255)
                   accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                             <  2:1355571/148‑1 (MQ=255)
                   accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                             <  1:1733886/148‑1 (MQ=255)
                   accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                             >  1:3705577/1‑148 (MQ=255)
                    ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                            <  1:1260001/148‑1 (MQ=255)
                     cgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAtt                                                                                                                           >  2:3255181/1‑148 (MQ=255)
                        cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAt                                                                                                                        <  2:610666/148‑1 (MQ=255)
                        cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAt                                                                                                                        <  2:3329140/148‑1 (MQ=255)
                          cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATca                                                                                                                      <  2:853816/148‑1 (MQ=255)
                          cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATca                                                                                                                      >  1:396264/1‑148 (MQ=255)
                          cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATca                                                                                                                      >  1:1505877/1‑148 (MQ=255)
                           ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                     >  1:2993291/1‑148 (MQ=255)
                           ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                     <  1:2705689/148‑1 (MQ=255)
                            cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacc                                                                                                                    >  1:3328912/1‑148 (MQ=255)
                            cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacc                                                                                                                    <  1:3516871/148‑1 (MQ=255)
                               ttttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                 <  1:664429/148‑1 (MQ=255)
                               ttttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                 >  1:1633282/1‑148 (MQ=255)
                                   ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                             <  1:668994/148‑1 (MQ=255)
                                   ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                             <  1:668710/148‑1 (MQ=255)
                                    tGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                            <  1:3022592/148‑1 (MQ=255)
                                    tGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                            <  2:1711353/148‑1 (MQ=255)
                                        tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTNCGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                        <  1:1932529/148‑1 (MQ=255)
                                         ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                       >  1:2979680/1‑148 (MQ=255)
                                         ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                       <  2:2305069/148‑1 (MQ=255)
                                          tCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGttt                                                                                                      <  1:2258971/148‑1 (MQ=255)
                                            aGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTag                                                                                                    <  2:3154409/148‑1 (MQ=255)
                                             gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                   >  2:2105232/1‑148 (MQ=255)
                                              atcatcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTagag                                                                                                  >  2:2509712/1‑148 (MQ=255)
                                                catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                >  2:2702191/1‑148 (MQ=255)
                                                catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                <  2:2009395/148‑1 (MQ=255)
                                                 atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                               >  2:1707698/1‑148 (MQ=255)
                                                 atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                               <  1:3292254/148‑1 (MQ=255)
                                                 atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                               <  1:2080201/148‑1 (MQ=255)
                                                      cTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                          >  1:994747/1‑148 (MQ=255)
                                                      cTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                          >  2:3567060/1‑148 (MQ=255)
                                                      cTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                          >  2:3805590/1‑148 (MQ=255)
                                                       tGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGAGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCATCGAc                                                                                         >  2:2551552/1‑148 (MQ=255)
                                                         ttttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGc                                                                                       >  1:475819/1‑148 (MQ=255)
                                                            tAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAg                                                                                    >  2:719351/1‑148 (MQ=255)
                                                              aTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                  >  2:2938572/1‑148 (MQ=255)
                                                              aTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                  >  2:1568503/1‑148 (MQ=255)
                                                                   gCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCg                                                                             <  1:3255181/148‑1 (MQ=255)
                                                                           tACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAttt                                                                     >  2:131386/1‑148 (MQ=255)
                                                                             ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                   >  2:1892992/1‑148 (MQ=255)
                                                                             ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                   <  1:1748965/148‑1 (MQ=255)
                                                                             acTTTTTTCACCAGCGCATGAATACCAGAAATGGTGTACGGGGTTGATAAATCGTATTTTTTCTTGCTCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                   <  2:3585675/147‑1 (MQ=255)
                                                                               ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                 >  2:3247140/1‑148 (MQ=255)
                                                                                 ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCgg                                                               <  2:2156804/148‑1 (MQ=255)
                                                                                     cACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtttt                                                           <  1:1377328/148‑1 (MQ=255)
                                                                                      aCCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTa                                                          <  2:1646191/148‑1 (MQ=255)
                                                                                       ccAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                         <  2:2247823/148‑1 (MQ=255)
                                                                                            gcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACg                                                    <  2:2629509/148‑1 (MQ=255)
                                                                                             ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                   <  2:1099314/148‑1 (MQ=255)
                                                                                             ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                   <  1:3742497/148‑1 (MQ=255)
                                                                                             ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                   <  2:1339604/148‑1 (MQ=255)
                                                                                             ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                   >  1:2416580/1‑148 (MQ=255)
                                                                                               tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                 >  2:1952523/1‑148 (MQ=255)
                                                                                               tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                 <  1:3389411/148‑1 (MQ=255)
                                                                                                 aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCa                                               <  2:93593/148‑1 (MQ=255)
                                                                                                   tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAAc                                             >  2:2993127/1‑148 (MQ=255)
                                                                                                     ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGc                                           >  1:3023826/1‑148 (MQ=255)
                                                                                                       aGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCa                                         <  2:877226/148‑1 (MQ=255)
                                                                                                        gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                        >  2:3702483/1‑148 (MQ=255)
                                                                                                        gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                        >  2:3702136/1‑148 (MQ=255)
                                                                                                         aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                       <  1:1653465/148‑1 (MQ=255)
                                                                                                         aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                       <  1:881740/148‑1 (MQ=255)
                                                                                                            tGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATg                                    >  2:3171969/1‑148 (MQ=255)
                                                                                                            tGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAACATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATg                                    >  2:1708782/1‑148 (MQ=255)
                                                                                                               tgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGttt                                 <  2:2344857/148‑1 (MQ=255)
                                                                                                                 tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                               <  1:2756771/148‑1 (MQ=255)
                                                                                                                 tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                               <  2:1383504/148‑1 (MQ=255)
                                                                                                                  aCGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGc                              >  1:1888937/1‑148 (MQ=255)
                                                                                                                         tGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                       <  2:3417335/148‑1 (MQ=255)
                                                                                                                          gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                      <  2:1277442/148‑1 (MQ=255)
                                                                                                                           agaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTg                     >  2:2695093/1‑148 (MQ=255)
                                                                                                                               aTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCa                 <  1:583461/148‑1 (MQ=255)
                                                                                                                               aTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCa                 <  1:585505/148‑1 (MQ=255)
                                                                                                                                tCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaa                <  2:1505877/148‑1 (MQ=255)
                                                                                                                                 cGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa               <  1:311076/148‑1 (MQ=255)
                                                                                                                                 cGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa               >  2:3756039/1‑148 (MQ=255)
                                                                                                                                   tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt             >  1:2624901/1‑148 (MQ=255)
                                                                                                                                   tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt             <  2:1403482/148‑1 (MQ=255)
                                                                                                                                   tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt             >  1:3546143/1‑148 (MQ=255)
                                                                                                                                   tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt             >  1:2639400/1‑148 (MQ=255)
                                                                                                                                    aTTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTc            <  1:2623054/148‑1 (MQ=255)
                                                                                                                                    aTTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTc            >  2:3099898/1‑148 (MQ=255)
                                                                                                                                      tttttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCa          >  2:3550954/1‑148 (MQ=255)
                                                                                                                                         ttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt       <  2:1762986/148‑1 (MQ=255)
                                                                                                                                            ttGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaa    >  1:2772083/1‑148 (MQ=255)
                                                                                                                                              gcgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaaa  <  2:43845/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAA  >  CP009273/1993383‑1993672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993381‑1993679
                                                                                                                                                     |                                                                                                                                                     
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTC                                                                                                                                                       >  SRR19973069.51753/1‑150 (MQ=60)
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973069.1997612/1‑150 (MQ=60)
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973069.1997561/1‑150 (MQ=60)
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973069.1997242/1‑150 (MQ=60)
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973069.1840116/1‑150 (MQ=60)
          TTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATG                                                                                                                                             <  SRR19973069.1647529/150‑1 (MQ=60)
             GTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTG                                                                                                                                          <  SRR19973069.2217755/150‑1 (MQ=60)
              TGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGA                                                                                                                                         <  SRR19973069.2504070/150‑1 (MQ=60)
              TGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGA                                                                                                                                         <  SRR19973069.2863545/150‑1 (MQ=60)
                  CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                     <  SRR19973069.3486225/150‑1 (MQ=60)
                   AGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGA                                                                                                                                    >  SRR19973069.3729787/1‑150 (MQ=60)
                    gaccgagcacctaTTTTTTGCATTTCAGATCATCGGCTGTTTTATTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   <  SRR19973069.3455193/137‑1 (MQ=60)
                    GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   <  SRR19973069.1971066/150‑1 (MQ=60)
                    GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   <  SRR19973069.1681068/150‑1 (MQ=60)
                     ACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATT                                                                                                                                  <  SRR19973069.1746045/150‑1 (MQ=60)
                      CCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTA                                                                                                                                 <  SRR19973069.1268499/150‑1 (MQ=60)
                          CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             >  SRR19973069.399169/1‑150 (MQ=60)
                          CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             >  SRR19973069.2647115/1‑150 (MQ=60)
                          CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             >  SRR19973069.1516308/1‑150 (MQ=60)
                           CCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCAC                                                                                                                            >  SRR19973069.3012703/1‑150 (MQ=60)
                           CCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCAC                                                                                                                            >  SRR19973069.3013165/1‑150 (MQ=60)
                            CCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACC                                                                                                                           >  SRR19973069.3350725/1‑150 (MQ=60)
                             CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973069.2723829/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         <  SRR19973069.2402686/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         <  SRR19973069.3539565/150‑1 (MQ=60)
                               ACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACA                                                                                                                        >  SRR19973069.1644472/1‑150 (MQ=60)
                               ACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACA                                                                                                                        >  SRR19973069.1644454/1‑150 (MQ=60)
                                 TTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATC                                                                                                                      <  SRR19973069.668940/150‑1 (MQ=60)
                                 TTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATC                                                                                                                      <  SRR19973069.668984/150‑1 (MQ=60)
                                     TTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATA                                                                                                                  <  SRR19973069.673281/150‑1 (MQ=60)
                                     TTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATA                                                                                                                  <  SRR19973069.673566/150‑1 (MQ=60)
                                     TTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATA                                                                                                                  <  SRR19973069.673460/150‑1 (MQ=60)
                                      TGCCTTTCAGATCATCGTCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAC                                                                                                                 <  SRR19973069.2672510/150‑1 (MQ=60)
                                      TGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAC                                                                                                                 <  SRR19973069.3042639/150‑1 (MQ=60)
                                      TGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAC                                                                                                                 <  SRR19973069.742652/150‑1 (MQ=60)
                                         CTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTT                                                                                                              >  SRR19973069.2999460/1‑150 (MQ=60)
                                          TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTNCGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973069.1945792/150‑1 (MQ=60)
                                            TCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAG                                                                                                           <  SRR19973069.336787/150‑1 (MQ=60)
                                            TCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAG                                                                                                           <  SRR19973069.2274246/150‑1 (MQ=60)
                                                   ATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAG                                                                                                    <  SRR19973069.3313813/150‑1 (MQ=60)
                                                   ATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAG                                                                                                    <  SRR19973069.2094593/150‑1 (MQ=60)
                                                   ATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTGTAGAGTCCAG                                                                                                    >  SRR19973069.1494631/1‑150 (MQ=60)
                                                     CGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCG                                                                                                  >  SRR19973069.1686551/1‑150 (MQ=60)
                                                      GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGA                                                                                                 >  SRR19973069.1001521/1‑150 (MQ=60)
                                                         TGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGC                                                                                              >  SRR19973069.479277/1‑150 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           >  SRR19973069.151935/1‑150 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           >  SRR19973069.376937/1‑150 (MQ=60)
                                                             TTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGC                                                                                          >  SRR19973069.2070225/1‑150 (MQ=60)
                                                                     GCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTC                                                                                  <  SRR19973069.3276500/150‑1 (MQ=60)
                                                                          CGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATT                                                                             <  SRR19973069.321570/150‑1 (MQ=60)
                                                                           GTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTT                                                                            >  SRR19973069.2479680/1‑150 (MQ=60)
                                                                             TACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGG                                                                          >  SRR19973069.3830281/1‑150 (MQ=60)
                                                                               CCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGATAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGATTCCAGCGACGCCAGCAAACCGTCCCATTTGGTC                                                                        <  SRR19973069.1760281/150‑1 (MQ=60)
                                                                               CCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTC                                                                        <  SRR19973069.1761275/150‑1 (MQ=60)
                                                                                       CACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAG                                                                <  SRR19973069.3029447/150‑1 (MQ=60)
                                                                                       CACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAG                                                                <  SRR19973069.1386930/150‑1 (MQ=60)
                                                                                        ACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGT                                                               >  SRR19973069.2785224/1‑150 (MQ=60)
                                                                                           AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                            >  SRR19973069.2840885/1‑150 (MQ=60)
                                                                                           AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                            <  SRR19973069.3503947/150‑1 (MQ=60)
                                                                                             CGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGC                                                          >  SRR19973069.2432907/1‑150 (MQ=60)
                                                                                              GCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCC                                                         <  SRR19973069.2526437/150‑1 (MQ=60)
                                                                                               CCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                        <  SRR19973069.3766887/150‑1 (MQ=60)
                                                                                               CCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                        >  SRR19973069.44410/1‑150 (MQ=60)
                                                                                                CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973069.1933774/1‑150 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                      >  SRR19973069.2126678/1‑150 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                      <  SRR19973069.3411550/150‑1 (MQ=60)
                                                                                                     TACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGC                                                  >  SRR19973069.3043882/1‑150 (MQ=60)
                                                                                                     TACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGC                                                  >  SRR19973069.3043873/1‑150 (MQ=60)
                                                                                                         AGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAG                                              >  SRR19973069.96694/1‑150 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973069.887847/150‑1 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973069.1664903/150‑1 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973069.1664923/150‑1 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            >  SRR19973069.1784178/1‑150 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCAACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973069.2218290/150‑1 (MQ=60)
                                                                                                                 TGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTG                                      <  SRR19973069.3280247/150‑1 (MQ=60)
                                                                                                                 TGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTG                                      <  SRR19973069.622034/150‑1 (MQ=60)
                                                                                                                  GTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGC                                     >  SRR19973069.1902045/1‑150 (MQ=60)
                                                                                                                   TACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCC                                    <  SRR19973069.2775176/150‑1 (MQ=60)
                                                                                                                           TGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTG                            <  SRR19973069.359579/150‑1 (MQ=60)
                                                                                                                                 ATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAA                      <  SRR19973069.589509/150‑1 (MQ=60)
                                                                                                                                 ATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAA                      <  SRR19973069.587456/150‑1 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973069.1663343/1‑150 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973069.2642457/1‑150 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    <  SRR19973069.313155/150‑1 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    <  SRR19973069.312630/150‑1 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973069.2642462/1‑150 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973069.2657074/1‑150 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973069.3569174/1‑150 (MQ=60)
                                                                                                                                      ATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCA                 <  SRR19973069.2640598/150‑1 (MQ=60)
                                                                                                                                       TTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCAC                >  SRR19973069.2895562/1‑150 (MQ=60)
                                                                                                                                            TCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAA           >  SRR19973069.2790552/1‑150 (MQ=60)
                                                                                                                                            TCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAA           >  SRR19973069.3553113/1‑150 (MQ=60)
                                                                                                                                                 CGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAACTTCCACTTCAAAACCG      >  SRR19973069.40823/1‑150 (MQ=60)
                                                                                                                                                   CTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGT    >  SRR19973069.609063/1‑150 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   <  SRR19973069.3707632/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   <  SRR19973069.1749115/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   <  SRR19973069.1748818/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   >  SRR19973069.2085131/1‑150 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   >  SRR19973069.2087490/1‑150 (MQ=60)
                                                                                                                                                     AATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  SRR19973069.528809/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993381‑1993679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑