Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,218,022 C→A 100% E110* (GAA→TAA)  pbpG ← D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,218,0220CA99.1% 733.7 / ‑1.8 223E110* (GAA→TAA) pbpGD‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (110/111);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

TTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCAC  >  CP009273/2217877‑2218166
                                                                                                                                                 |                                                                                                                                                
ttgttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGc                                                                                                                                                >  1:1211127/1‑148 (MQ=255)
  gttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTa                                                                                                                                              >  2:3797487/1‑148 (MQ=255)
   ttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTAt                                                                                                                                             >  2:3789435/1‑148 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         >  2:2155239/1‑148 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         <  1:508631/148‑1 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         <  1:1370745/148‑1 (MQ=255)
        tCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGt                                                                                                                                        <  2:1786099/148‑1 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  2:1104872/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  2:3540872/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  1:2714684/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  1:2006664/1‑148 (MQ=255)
                  ttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgcg                                                                                                                              >  1:2411043/1‑148 (MQ=255)
                   tcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGa                                                                                                                             <  1:2086185/148‑1 (MQ=255)
                    cgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGaa                                                                                                                            <  1:2490067/148‑1 (MQ=255)
                    cgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGaa                                                                                                                            <  1:312442/148‑1 (MQ=255)
                     gctttcgcATTCATTGCCTTAATAACGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAt                                                                                                                           <  1:2283562/148‑1 (MQ=255)
                     gctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAt                                                                                                                           <  1:356944/148‑1 (MQ=255)
                       tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAg                                                                                                                         <  2:922748/148‑1 (MQ=255)
                       tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAg                                                                                                                         <  1:1620279/148‑1 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   >  1:2563270/1‑148 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   <  2:2885378/148‑1 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   <  2:1007105/148‑1 (MQ=255)
                              ttcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCtt                                                                                                                  <  2:1456988/148‑1 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTGTTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 <  1:2364349/148‑1 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 >  1:3773546/1‑148 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 >  2:2372720/1‑148 (MQ=255)
                                cattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTc                                                                                                                <  1:3520082/148‑1 (MQ=255)
                                 attGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCa                                                                                                               <  2:129500/148‑1 (MQ=255)
                                  ttGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAt                                                                                                              >  1:116937/1‑148 (MQ=255)
                                  ttGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAt                                                                                                              <  2:1497213/148‑1 (MQ=255)
                                   tGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtc                                                                                                             >  2:354683/1‑148 (MQ=255)
                                     ccTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtctc                                                                                                           <  1:2787873/148‑1 (MQ=255)
                                     ccTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtctc                                                                                                           <  1:2787393/148‑1 (MQ=255)
                                           taaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                     <  2:444766/148‑1 (MQ=255)
                                           taaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                     >  1:502929/1‑148 (MQ=255)
                                               ggCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 <  1:897802/148‑1 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 >  1:3680404/1‑147 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 <  2:3680404/147‑1 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGc                                                                                                <  1:420744/148‑1 (MQ=255)
                                                 catagTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               >  2:3434192/5‑148 (MQ=255)
                                                 cTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               <  2:1625023/148‑1 (MQ=255)
                                                 cTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               >  2:3433684/1‑148 (MQ=255)
                                                  tttGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTg                                                                                              <  2:1351999/148‑1 (MQ=255)
                                                     gTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGata                                                                                           <  2:1148997/148‑1 (MQ=255)
                                                      tAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGatat                                                                                          <  2:1364568/148‑1 (MQ=255)
                                                       aaCCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATc                                                                                         >  2:2451402/1‑148 (MQ=255)
                                                         ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                       >  2:600179/1‑148 (MQ=255)
                                                         ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                       >  2:1536775/1‑148 (MQ=255)
                                                            ccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACtt                                                                                    >  1:1124066/1‑148 (MQ=255)
                                                                gATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAg                                                                                >  1:3423776/1‑148 (MQ=255)
                                                                gATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAg                                                                                <  2:63859/148‑1 (MQ=255)
                                                                 aTAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGt                                                                               <  1:1656400/148‑1 (MQ=255)
                                                                  tAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGtt                                                                              >  2:1195003/1‑148 (MQ=255)
                                                                     tGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGttttt                                                                           >  1:3249755/1‑148 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          >  1:1064960/1‑148 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          <  2:3075652/148‑1 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          <  2:3199074/148‑1 (MQ=255)
                                                                         gcgcAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATc                                                                       <  1:2060688/148‑1 (MQ=255)
                                                                          cgcAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATcc                                                                      <  2:3692672/148‑1 (MQ=255)
                                                                             aaGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGc                                                                   >  2:159588/1‑148 (MQ=255)
                                                                               ggCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCACCCAGCgg                                                                 <  2:1210538/148‑1 (MQ=255)
                                                                                gCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGc                                                                >  2:1754446/1‑148 (MQ=255)
                                                                                          gcgcgGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCAt                                                      >  2:2499500/1‑148 (MQ=255)
                                                                                                ttttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGca                                                >  2:84675/1‑148 (MQ=255)
                                                                                                 tttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGcac                                               >  2:1290310/1‑148 (MQ=255)
                                                                                                 tttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGcac                                               >  2:262850/1‑148 (MQ=255)
                                                                                                   tCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACaa                                             <  1:2155239/148‑1 (MQ=255)
                                                                                                          aCATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATcgc                                      >  2:2815236/1‑148 (MQ=255)
                                                                                                            aTCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCgg                                    <  1:2882446/148‑1 (MQ=255)
                                                                                                            aTCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCgg                                    >  1:2241919/1‑148 (MQ=255)
                                                                                                               aGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCa                                 <  1:2143034/148‑1 (MQ=255)
                                                                                                                gcgcCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCAt                                <  2:858091/148‑1 (MQ=255)
                                                                                                                gcgcCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCAt                                <  2:2381270/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  2:2563270/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  1:1741012/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  2:765731/148‑1 (MQ=255)
                                                                                                                    cAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAt                            <  2:2812366/148‑1 (MQ=255)
                                                                                                                     aGCAACATCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                           <  2:2048196/148‑1 (MQ=255)
                                                                                                                     aGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                           <  1:413204/148‑1 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         >  1:641707/1‑148 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         <  1:1956579/148‑1 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         <  1:1957497/148‑1 (MQ=255)
                                                                                                                        aaCAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                        <  1:2431641/148‑1 (MQ=255)
                                                                                                                        aaCAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                        <  2:3616664/148‑1 (MQ=255)
                                                                                                                          cAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTg                      >  1:1351156/1‑148 (MQ=255)
                                                                                                                          cAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTg                      >  2:2365108/1‑148 (MQ=255)
                                                                                                                           aGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGa                     >  1:2325823/1‑148 (MQ=255)
                                                                                                                           aGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTGTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGa                     >  2:780283/1‑148 (MQ=255)
                                                                                                                            gCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGAt                    >  2:305417/1‑148 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 >  1:2966101/1‑148 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 <  1:729323/148‑1 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATATAGCTATTCAGTCGTACTCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCAACCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 >  2:677087/1‑148 (MQ=255)
                                                                                                                                   tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCa             <  2:502454/148‑1 (MQ=255)
                                                                                                                                   tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCa             <  2:2428963/148‑1 (MQ=255)
                                                                                                                                    ttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa            <  1:3797487/148‑1 (MQ=255)
                                                                                                                                    ttACGGCTGAGTTAGCTATTCACTCGTACGCGCGAATAGACCCCGTTCATCTCCGGCGTCTGGCTGATATCCACTTTTCGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCGGATAGACGCaa            <  2:2103089/148‑1 (MQ=255)
                                                                                                                                     tACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAAt           <  1:537722/148‑1 (MQ=255)
                                                                                                                                      aCGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATc          <  2:988836/148‑1 (MQ=255)
                                                                                                                                        ggCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCgg        <  1:3789435/148‑1 (MQ=255)
                                                                                                                                             aTTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCa   <  2:1392176/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCAC  >  CP009273/2217877‑2218166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACCAGAT  >  CP009273/2217873‑2218171
                                                                                                                                                     |                                                                                                                                                     
CGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTA                                                                                                                                                       >  SRR19973069.995542/1‑150 (MQ=60)
 GTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAG                                                                                                                                                      <  SRR19973069.589042/150‑1 (MQ=60)
 GTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAG                                                                                                                                                      <  SRR19973069.588970/150‑1 (MQ=60)
  TGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGC                                                                                                                                                     >  SRR19973069.1219438/1‑150 (MQ=60)
   GTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCT                                                                                                                                                    <  SRR19973069.3812574/150‑1 (MQ=60)
     TGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTAT                                                                                                                                                  >  SRR19973069.382024/1‑150 (MQ=60)
       TTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTC                                                                                                                                                <  SRR19973069.3489561/150‑1 (MQ=60)
         CATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAG                                                                                                                                              >  SRR19973069.3159089/1‑150 (MQ=60)
           TTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTC                                                                                                                                            <  SRR19973069.512257/150‑1 (MQ=60)
           TTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTC                                                                                                                                            <  SRR19973069.1380280/150‑1 (MQ=60)
                   GATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGC                                                                                                                                    >  SRR19973069.2732879/1‑150 (MQ=60)
                   GATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGC                                                                                                                                    >  SRR19973069.926708/1‑150 (MQ=60)
                   GATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGC                                                                                                                                    >  SRR19973069.2020630/1‑150 (MQ=60)
                    ATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTTTAACCACCGGGATAATTGTGCGCAAGTCTTGCCGCCGCGCGGTTTTCAGAAGACATCATCGCCAGCAACATCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCG                                                                                                                                   >  SRR19973069.856897/1‑150 (MQ=60)
                    ATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTAAGTCGTACGCGCG                                                                                                                                   >  SRR19973069.2427342/1‑150 (MQ=60)
                       TCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAT                                                                                                                                <  SRR19973069.2100682/150‑1 (MQ=60)
                       TCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAT                                                                                                                                <  SRR19973069.2100617/150‑1 (MQ=60)
                        CGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATA                                                                                                                               <  SRR19973069.314522/150‑1 (MQ=60)
                        CGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATA                                                                                                                               <  SRR19973069.2506725/150‑1 (MQ=60)
                        CGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATA                                                                                                                               <  SRR19973069.3764071/150‑1 (MQ=60)
                         GCTTTCGCATTCATTGCCTTAATAACGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAG                                                                                                                              <  SRR19973069.2299065/150‑1 (MQ=60)
                         GCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAG                                                                                                                              <  SRR19973069.359501/150‑1 (MQ=60)
                         GCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAG                                                                                                                              <  SRR19973069.2296797/150‑1 (MQ=60)
                         GCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAG                                                                                                                              >  SRR19973069.116125/1‑150 (MQ=60)
                           TTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGAC                                                                                                                            <  SRR19973069.1629962/150‑1 (MQ=60)
                           TTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGAC                                                                                                                            <  SRR19973069.1631260/150‑1 (MQ=60)
                               GCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCT                                                                                                                        >  SRR19973069.652554/1‑150 (MQ=60)
                               GCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCT                                                                                                                        >  SRR19973069.2580197/1‑150 (MQ=60)
                                 ATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTT                                                                                                                      >  SRR19973069.512148/1‑150 (MQ=60)
                                 ATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTT                                                                                                                      >  SRR19973069.3798059/1‑150 (MQ=60)
                                   TCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTGTTCAGTCGTACGCGCGAATAGACCCCTTTCA                                                                                                                    <  SRR19973069.2380408/150‑1 (MQ=60)
                                    CATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAT                                                                                                                   >  SRR19973069.118069/1‑150 (MQ=60)
                                    CATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAT                                                                                                                   <  SRR19973069.3542822/150‑1 (MQ=60)
                                    CATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAT                                                                                                                   <  SRR19973069.3542827/150‑1 (MQ=60)
                                    CATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAT                                                                                                                   >  SRR19973069.367607/1‑150 (MQ=60)
                                        GCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCC                                                                                                               >  SRR19973069.2299839/1‑150 (MQ=60)
                                         CCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCG                                                                                                              <  SRR19973069.2806422/150‑1 (MQ=60)
                                         CCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCG                                                                                                              <  SRR19973069.2806403/150‑1 (MQ=60)
                                         CCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCG                                                                                                              <  SRR19973069.2805939/150‑1 (MQ=60)
                                          CTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGG                                                                                                             >  SRR19973069.2075605/1‑150 (MQ=60)
                                            TAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCG                                                                                                           >  SRR19973069.2069015/1‑150 (MQ=60)
                                             AATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGT                                                                                                          >  SRR19973069.506536/1‑150 (MQ=60)
                                             AATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGT                                                                                                          >  SRR19973069.506056/1‑150 (MQ=60)
                                             AATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGT                                                                                                          >  SRR19973069.2445346/1‑150 (MQ=60)
                                                AAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTG                                                                                                       >  SRR19973069.3367625/1‑150 (MQ=60)
                                                AAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTG                                                                                                       >  SRR19973069.3840425/1‑150 (MQ=60)
                                                  AGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGC                                                                                                     >  SRR19973069.3704477/1‑150 (MQ=60)
                                                  AGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGC                                                                                                     >  SRR19973069.3704587/1‑150 (MQ=60)
                                                   GGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCT                                                                                                    <  SRR19973069.903983/150‑1 (MQ=60)
                                                    GCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTG                                                                                                   <  SRR19973069.423835/150‑1 (MQ=60)
                                                     CTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGA                                                                                                  >  SRR19973069.1667202/1‑150 (MQ=60)
                                                           AACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCA                                                                                            >  SRR19973069.1703077/1‑150 (MQ=60)
                                                              CACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTT                                                                                         >  SRR19973069.1131905/1‑150 (MQ=60)
                                                              CACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTT                                                                                         >  SRR19973069.2534996/1‑150 (MQ=60)
                                                                 CGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTA                                                                                      <  SRR19973069.2403833/150‑1 (MQ=60)
                                                                  GGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAG                                                                                     >  SRR19973069.3446049/1‑150 (MQ=60)
                                                                     ATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTT                                                                                  <  SRR19973069.1667864/150‑1 (MQ=60)
                                                                       AATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTT                                                                                >  SRR19973069.3271029/1‑150 (MQ=60)
                                                                        ATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTC                                                                               >  SRR19973069.1072376/1‑150 (MQ=60)
                                                                           GTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATC                                                                            <  SRR19973069.3093186/150‑1 (MQ=60)
                                                                           GTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATC                                                                            <  SRR19973069.2847394/150‑1 (MQ=60)
                                                                             GCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCA                                                                          <  SRR19973069.2074979/150‑1 (MQ=60)
                                                                                CAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCG                                                                       <  SRR19973069.454952/150‑1 (MQ=60)
                                                                                CAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTGTTAGTTTTTCATCCAGCG                                                                       >  SRR19973069.2501870/1‑150 (MQ=60)
                                                                                 AAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGG                                                                      >  SRR19973069.1938340/1‑150 (MQ=60)
                                                                                 AAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGG                                                                      >  SRR19973069.1361295/1‑150 (MQ=60)
                                                                                 AAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGG                                                                      >  SRR19973069.3276872/1‑150 (MQ=60)
                                                                                              GCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCC                                                         <  SRR19973069.1190565/150‑1 (MQ=60)
                                                                                                  GGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCA                                                     >  SRR19973069.1323315/1‑150 (MQ=60)
                                                                                                       TCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACC                                                <  SRR19973069.2169995/150‑1 (MQ=60)
                                                                                                              ACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGG                                         >  SRR19973069.2257025/1‑150 (MQ=60)
                                                                                                               CATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGT                                        <  SRR19973069.2467890/150‑1 (MQ=60)
                                                                                                                ATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGGTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATGGCGGTC                                       >  SRR19973069.235394/1‑150 (MQ=60)
                                                                                                                ATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTC                                       <  SRR19973069.2901387/150‑1 (MQ=60)
                                                                                                                ATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTC                                       <  SRR19973069.1988091/150‑1 (MQ=60)
                                                                                                                   AGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATT                                    <  SRR19973069.2157749/150‑1 (MQ=60)
                                                                                                                       CCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATT                                <  SRR19973069.1753228/150‑1 (MQ=60)
                                                                                                                        CAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTT                               <  SRR19973069.2712157/150‑1 (MQ=60)
                                                                                                                         AGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTG                              >  SRR19973069.645917/1‑150 (MQ=60)
                                                                                                                         AGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTG                              >  SRR19973069.646081/1‑150 (MQ=60)
                                                                                                                         AGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTG                              <  SRR19973069.416229/150‑1 (MQ=60)
                                                                                                                           CAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGTCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCT                            <  SRR19973069.1971036/150‑1 (MQ=60)
                                                                                                                           CAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCT                            <  SRR19973069.1971060/150‑1 (MQ=60)
                                                                                                                           CAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCT                            <  SRR19973069.1970131/150‑1 (MQ=60)
                                                                                                                            AACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTG                           >  SRR19973069.1783321/1‑150 (MQ=60)
                                                                                                                            AACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTG                           >  SRR19973069.1360515/1‑150 (MQ=60)
                                                                                                                            AACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTG                           >  SRR19973069.1360835/1‑150 (MQ=60)
                                                                                                                            AACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTG                           <  SRR19973069.2448040/150‑1 (MQ=60)
                                                                                                                            AACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTG                           >  SRR19973069.521829/1‑150 (MQ=60)
                                                                                                                             ACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGA                          >  SRR19973069.2341642/1‑150 (MQ=60)
                                                                                                                               AGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATA                        >  SRR19973069.2861933/1‑150 (MQ=60)
                                                                                                                               AGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATA                        >  SRR19973069.2983451/1‑150 (MQ=60)
                                                                                                                                GCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAG                       <  SRR19973069.2743310/150‑1 (MQ=60)
                                                                                                                                 CATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGA                      >  SRR19973069.2985789/1‑150 (MQ=60)
                                                                                                                                  ATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGAC                     <  SRR19973069.10996/150‑1 (MQ=60)
                                                                                                                                   TATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACG                    <  SRR19973069.734479/150‑1 (MQ=60)
                                                                                                                                   TATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACG                    >  SRR19973069.423746/1‑150 (MQ=60)
                                                                                                                                      CTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAA                 >  SRR19973069.2312334/1‑150 (MQ=60)
                                                                                                                                        TTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATC               <  SRR19973069.3822090/150‑1 (MQ=60)
                                                                                                                                         TACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCG              <  SRR19973069.541517/150‑1 (MQ=60)
                                                                                                                                         TACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCG              <  SRR19973069.541511/150‑1 (MQ=60)
                                                                                                                                            GGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGAC           <  SRR19973069.3814010/150‑1 (MQ=60)
                                                                                                                                            GGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGAC           <  SRR19973069.3814008/150‑1 (MQ=60)
                                                                                                                                            GGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGAC           >  SRR19973069.2159508/1‑150 (MQ=60)
                                                                                                                                            GGCTGATTTAGCTATTCAGTAGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATACAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGAC           >  SRR19973069.2136757/1‑150 (MQ=60)
                                                                                                                                                  TTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACCA     <  SRR19973069.225883/150‑1 (MQ=60)
                                                                                                                                                   TTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACCAG    <  SRR19973069.1594156/150‑1 (MQ=60)
                                                                                                                                                     AGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACCAGAT  >  SRR19973069.2778384/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACCAGAT  >  CP009273/2217873‑2218171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑