Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,223,247 C→T 100% W206* (TGG→TGA)  dusC ← tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,223,2470CT97.7% 701.2 / 3.9 214W206* (TGG→TGA) dusCtRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base C (5/0);  new base T (88/121);  total (93/121)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

TGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTG  >  CP009273/2223104‑2223390
                                                                                                                                               |                                                                                                                                               
  cagcaAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTcc                                                                                                                                           <  1:2482064/148‑1 (MQ=255)
   agcaaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTccc                                                                                                                                          <  1:615841/148‑1 (MQ=255)
     caaagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAg                                                                                                                                        >  1:2220648/1‑148 (MQ=255)
        agcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGAtt                                                                                                                                     >  1:3414182/1‑148 (MQ=255)
          caaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTc                                                                                                                                   <  1:2433169/148‑1 (MQ=255)
           aaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCNGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCa                                                                                                                                  <  1:1937023/148‑1 (MQ=255)
           aaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCa                                                                                                                                  <  2:1909203/148‑1 (MQ=255)
            accaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAc                                                                                                                                 >  1:592132/1‑148 (MQ=255)
              caccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCg                                                                                                                               <  1:2356787/148‑1 (MQ=255)
               accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGt                                                                                                                              >  2:157049/1‑148 (MQ=255)
               accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGt                                                                                                                              >  1:611130/1‑148 (MQ=255)
                ccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                             >  2:2440847/1‑148 (MQ=255)
                ccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                             >  1:2513394/1‑148 (MQ=255)
                ccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGACTTCACCGtt                                                                                                                             >  1:3214504/1‑148 (MQ=255)
                 cTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTg                                                                                                                            <  2:2676427/148‑1 (MQ=255)
                    cGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCa                                                                                                                         <  2:3669875/148‑1 (MQ=255)
                       ccACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATc                                                                                                                      >  2:747866/1‑148 (MQ=255)
                        cACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                     <  1:1580730/148‑1 (MQ=255)
                        cACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                     <  1:3367728/148‑1 (MQ=255)
                            gCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCgg                                                                                                                 >  2:957635/1‑148 (MQ=255)
                             cATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGa                                                                                                                <  1:211104/148‑1 (MQ=255)
                                tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                             <  2:3719915/148‑1 (MQ=255)
                                tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                             <  2:913422/148‑1 (MQ=255)
                                tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAata                                                                                                             >  2:2779533/1‑148 (MQ=255)
                                     gTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAg                                                                                                        >  2:2638662/1‑148 (MQ=255)
                                      ttCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGc                                                                                                       <  1:3061716/148‑1 (MQ=255)
                                       tCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGcc                                                                                                      >  1:155355/1‑148 (MQ=255)
                                         gTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGc                                                                                                    <  1:3227550/148‑1 (MQ=255)
                                          ttATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCt                                                                                                   <  2:2087160/148‑1 (MQ=255)
                                           tataTTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTg                                                                                                  >  2:1539731/1‑148 (MQ=255)
                                              atTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCg                                                                                               >  2:2236689/1‑148 (MQ=255)
                                               ttttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGa                                                                                              <  1:871015/148‑1 (MQ=255)
                                                tttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGaa                                                                                             >  2:2336505/1‑148 (MQ=255)
                                                tttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGaa                                                                                             <  1:3778133/148‑1 (MQ=255)
                                                 ttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAat                                                                                            <  1:508967/148‑1 (MQ=255)
                                                   accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAatat                                                                                          <  2:1126327/148‑1 (MQ=255)
                                                   accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAatat                                                                                          <  1:715/148‑1 (MQ=255)
                                                   accaccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAatat                                                                                          <  1:669/148‑1 (MQ=255)
                                                      accCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGc                                                                                       <  1:2523279/148‑1 (MQ=255)
                                                      accCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGc                                                                                       <  2:704265/148‑1 (MQ=255)
                                                        ccGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCa                                                                                     <  2:3568278/148‑1 (MQ=255)
                                                         cGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCaa                                                                                    <  1:2145277/148‑1 (MQ=255)
                                                         cGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCaa                                                                                    >  2:1643560/1‑148 (MQ=255)
                                                            cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCg                                                                                 <  2:286588/148‑1 (MQ=255)
                                                            cTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCg                                                                                 <  2:2214311/148‑1 (MQ=255)
                                                              cAGGTTGGGAATATTGAGCGCCCCGCGACCACTCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGcc                                                                               <  1:764470/148‑1 (MQ=255)
                                                              cAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGcc                                                                               >  1:1810537/1‑148 (MQ=255)
                                                                ggTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTg                                                                             >  1:2852284/1‑148 (MQ=255)
                                                                ggTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTg                                                                             >  1:2157655/1‑148 (MQ=255)
                                                                  ttGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                           <  2:3130614/148‑1 (MQ=255)
                                                                   tGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                          <  2:592132/148‑1 (MQ=255)
                                                                     ggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGt                                                                        <  2:503339/148‑1 (MQ=255)
                                                                      gAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc                                                                       >  1:3682085/1‑148 (MQ=255)
                                                                      gAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc                                                                       >  1:1790825/1‑148 (MQ=255)
                                                                        atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCaa                                                                     <  2:2025812/148‑1 (MQ=255)
                                                                        atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCaa                                                                     <  2:1704783/148‑1 (MQ=255)
                                                                          atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata                                                                   >  2:1309834/1‑148 (MQ=255)
                                                                            tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg                                                                 >  1:2825038/1‑148 (MQ=255)
                                                                            tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg                                                                 <  2:1958263/148‑1 (MQ=255)
                                                                            tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg                                                                 <  1:2245492/148‑1 (MQ=255)
                                                                            tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg                                                                 >  2:3357340/1‑148 (MQ=255)
                                                                              aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                               <  1:1446419/148‑1 (MQ=255)
                                                                              aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                               >  2:2219729/1‑148 (MQ=255)
                                                                              aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                               <  2:1359453/148‑1 (MQ=255)
                                                                              aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                               >  1:2308599/1‑148 (MQ=255)
                                                                               gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTc                                                              <  1:2441284/148‑1 (MQ=255)
                                                                                       cgACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGt                                                      <  2:1500779/148‑1 (MQ=255)
                                                                                         aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCCGACCATATGCTCCGCGCGGTaa                                                    >  2:2955411/1‑148 (MQ=255)
                                                                                         aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTaa                                                    >  2:2955341/1‑148 (MQ=255)
                                                                                         aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTaa                                                    >  2:2955135/1‑148 (MQ=255)
                                                                                         aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTaa                                                    >  2:103010/1‑148 (MQ=255)
                                                                                          ccAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAc                                                   >  1:3125087/1‑148 (MQ=255)
                                                                                             atcatcACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCt                                                >  1:3154562/1‑148 (MQ=255)
                                                                                              tcatcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTg                                               >  2:3174160/1‑148 (MQ=255)
                                                                                              tcatcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTg                                               <  1:222889/148‑1 (MQ=255)
                                                                                               catcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGc                                              >  1:2107258/1‑148 (MQ=255)
                                                                                                atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGct                                             >  2:3293691/1‑148 (MQ=255)
                                                                                                atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGct                                             <  1:3576522/148‑1 (MQ=255)
                                                                                                atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGct                                             <  1:1683165/148‑1 (MQ=255)
                                                                                                atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGct                                             <  1:3575545/148‑1 (MQ=255)
                                                                                                 tcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctc                                            >  1:2022695/1‑148 (MQ=255)
                                                                                                   aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCtt                                          <  1:1151710/148‑1 (MQ=255)
                                                                                                   aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCtt                                          <  1:1053413/148‑1 (MQ=255)
                                                                                                    cTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt                                         >  2:550183/1‑148 (MQ=255)
                                                                                                    cTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt                                         <  2:1137130/148‑1 (MQ=255)
                                                                                                       cGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGt                                      <  2:1381823/148‑1 (MQ=255)
                                                                                                       cGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGt                                      <  2:44983/148‑1 (MQ=255)
                                                                                                         tCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTcc                                    >  2:2980882/1‑148 (MQ=255)
                                                                                                                 gCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGc                            >  2:1667604/1‑148 (MQ=255)
                                                                                                                  cTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGca                           >  1:3735225/1‑148 (MQ=255)
                                                                                                                   tGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcac                          <  1:2779533/148‑1 (MQ=255)
                                                                                                                     aTCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcacca                        >  1:2077990/1‑148 (MQ=255)
                                                                                                                            tGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTc                 <  1:957662/148‑1 (MQ=255)
                                                                                                                                 tgttgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAg            >  1:3543505/1‑148 (MQ=255)
                                                                                                                                          ctctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCt   >  2:2159133/1‑148 (MQ=255)
                                                                                                                                          ctctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCt   >  2:229354/1‑148 (MQ=255)
                                                                                                                                           tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg  <  2:2590601/148‑1 (MQ=255)
                                                                                                                                           tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg  <  2:1422734/148‑1 (MQ=255)
                                                                                                                                           tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg  >  2:1790172/1‑148 (MQ=255)
                                                                                                                                           tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg  <  1:2237012/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTG  >  CP009273/2223104‑2223390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGA  >  CP009273/2223100‑2223394
                                                                                                                                                   |                                                                                                                                                   
TTTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCA                                                                                                                                                   <  SRR19973069.420539/150‑1 (MQ=60)
TTTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCA                                                                                                                                                   <  SRR19973069.2356262/150‑1 (MQ=60)
 TTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAG                                                                                                                                                  <  SRR19973069.2891217/150‑1 (MQ=60)
 TTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAG                                                                                                                                                  <  SRR19973069.2890828/150‑1 (MQ=60)
      CAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCA                                                                                                                                             <  SRR19973069.2498682/150‑1 (MQ=60)
       AGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAG                                                                                                                                            >  SRR19973069.2235872/1‑150 (MQ=60)
       AGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAG                                                                                                                                            >  SRR19973069.2235670/1‑150 (MQ=60)
       AGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAG                                                                                                                                            <  SRR19973069.619945/150‑1 (MQ=60)
       AGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAG                                                                                                                                            <  SRR19973069.619949/150‑1 (MQ=60)
          AAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATT                                                                                                                                         >  SRR19973069.3436427/1‑150 (MQ=60)
              CAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAC                                                                                                                                     >  SRR19973069.596153/1‑150 (MQ=60)
              CAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAC                                                                                                                                     >  SRR19973069.596159/1‑150 (MQ=60)
              CAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAC                                                                                                                                     >  SRR19973069.596182/1‑150 (MQ=60)
              CAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAC                                                                                                                                     <  SRR19973069.2449577/150‑1 (MQ=60)
               AACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCNGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACC                                                                                                                                    <  SRR19973069.1950337/150‑1 (MQ=60)
                 CCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGT                                                                                                                                  >  SRR19973069.615217/1‑150 (MQ=60)
                  CACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTT                                                                                                                                 >  SRR19973069.2530130/1‑150 (MQ=60)
                  CACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTT                                                                                                                                 <  SRR19973069.2372806/150‑1 (MQ=60)
                  CACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGACTTCACCGTT                                                                                                                                 >  SRR19973069.3235479/1‑150 (MQ=60)
                            CACGGCATTCGCTGTTCTTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACC                                                                                                                       <  SRR19973069.3389836/150‑1 (MQ=60)
                            CACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACC                                                                                                                       <  SRR19973069.1817753/150‑1 (MQ=60)
                            CACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACC                                                                                                                       <  SRR19973069.1591455/150‑1 (MQ=60)
                            CACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACC                                                                                                                       <  SRR19973069.3313271/150‑1 (MQ=60)
                            CACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACC                                                                                                                       <  SRR19973069.3389748/150‑1 (MQ=60)
                               GGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGA                                                                                                                    >  SRR19973069.357445/1‑150 (MQ=60)
                               GGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGA                                                                                                                    >  SRR19973069.2190065/1‑150 (MQ=60)
                               GGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGA                                                                                                                    >  SRR19973069.251204/1‑150 (MQ=60)
                                 CATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAAT                                                                                                                  <  SRR19973069.212770/150‑1 (MQ=60)
                                   TTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATAT                                                                                                                <  SRR19973069.1505617/150‑1 (MQ=60)
                                    TCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATT                                                                                                               <  SRR19973069.2432864/150‑1 (MQ=60)
                                      GCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCA                                                                                                             <  SRR19973069.636156/150‑1 (MQ=60)
                                      GCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCA                                                                                                             <  SRR19973069.2032981/150‑1 (MQ=60)
                                         GTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCC                                                                                                          >  SRR19973069.2855678/1‑150 (MQ=60)
                                         GTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCC                                                                                                          >  SRR19973069.156721/1‑150 (MQ=60)
                                         GTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCC                                                                                                          >  SRR19973069.770922/1‑150 (MQ=60)
                                          TTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCG                                                                                                         <  SRR19973069.3081972/150‑1 (MQ=60)
                                          TTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCG                                                                                                         <  SRR19973069.3081967/150‑1 (MQ=60)
                                          TTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCG                                                                                                         >  SRR19973069.2112576/1‑150 (MQ=60)
                                          TTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCG                                                                                                         >  SRR19973069.170476/1‑150 (MQ=60)
                                             GTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTG                                                                                                      <  SRR19973069.3247893/150‑1 (MQ=60)
                                             GTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTG                                                                                                      <  SRR19973069.3248654/150‑1 (MQ=60)
                                                   TTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGANT                                                                                                <  SRR19973069.877074/150‑1 (MQ=60)
                                                    TTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATA                                                                                               <  SRR19973069.3802672/150‑1 (MQ=60)
                                                     TTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATAT                                                                                              <  SRR19973069.512594/150‑1 (MQ=60)
                                                      TACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATC                                                                                             <  SRR19973069.446625/150‑1 (MQ=60)
                                                       ACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCG                                                                                            <  SRR19973069.680/150‑1 (MQ=60)
                                                       ACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCG                                                                                            <  SRR19973069.726/150‑1 (MQ=60)
                                                          ACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCA                                                                                         <  SRR19973069.2540046/150‑1 (MQ=60)
                                                             CGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATC                                                                                      <  SRR19973069.2159996/150‑1 (MQ=60)
                                                              GGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCG                                                                                     <  SRR19973069.1654866/150‑1 (MQ=60)
                                                                CTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCC                                                                                   >  SRR19973069.1823496/1‑150 (MQ=60)
                                                                CTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCC                                                                                   >  SRR19973069.1823265/1‑150 (MQ=60)
                                                                 TCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCT                                                                                  <  SRR19973069.3715265/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCACTCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 <  SRR19973069.769864/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 <  SRR19973069.768870/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 >  SRR19973069.2871063/1‑150 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 <  SRR19973069.692281/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 >  SRR19973069.2871144/1‑150 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 <  SRR19973069.3207257/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 <  SRR19973069.2482318/150‑1 (MQ=60)
                                                                  CAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTG                                                                                 >  SRR19973069.2172428/1‑150 (MQ=60)
                                                                        GGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTC                                                                           >  SRR19973069.1803443/1‑150 (MQ=60)
                                                                        GGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTC                                                                           >  SRR19973069.2328548/1‑150 (MQ=60)
                                                                        GGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTC                                                                           >  SRR19973069.3706171/1‑150 (MQ=60)
                                                                         GGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCA                                                                          <  SRR19973069.2455589/150‑1 (MQ=60)
                                                                           AATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAAT                                                                        <  SRR19973069.1372963/150‑1 (MQ=60)
                                                                              ATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATG                                                                     >  SRR19973069.2843720/1‑150 (MQ=60)
                                                                              ATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATG                                                                     >  SRR19973069.2842180/1‑150 (MQ=60)
                                                                                tgctcttcctatctCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCT                                                                   <  SRR19973069.980473/136‑1 (MQ=60)
                                                                                TGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCT                                                                   <  SRR19973069.2260596/150‑1 (MQ=60)
                                                                                TGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCT                                                                   <  SRR19973069.2260610/150‑1 (MQ=60)
                                                                                TGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCT                                                                   >  SRR19973069.2324307/1‑150 (MQ=60)
                                                                                TGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCT                                                                   >  SRR19973069.2324309/1‑150 (MQ=60)
                                                                                  AGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCC                                                                 <  SRR19973069.1456514/150‑1 (MQ=60)
                                                                                   GCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCG                                                                <  SRR19973069.2457725/150‑1 (MQ=60)
                                                                                            GACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAC                                                       >  SRR19973069.2158487/1‑150 (MQ=60)
                                                                                            GACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAC                                                       >  SRR19973069.3146849/1‑150 (MQ=60)
                                                                                            GACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAC                                                       >  SRR19973069.3145601/1‑150 (MQ=60)
                                                                                               CAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCT                                                    <  SRR19973069.2457288/150‑1 (MQ=60)
                                                                                               CAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCT                                                    >  SRR19973069.3175167/1‑150 (MQ=60)
                                                                                               CAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTGGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCT                                                    <  SRR19973069.523254/150‑1 (MQ=60)
                                                                                                 ATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGACTATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTACCCCTGC                                                  >  SRR19973069.2121800/1‑150 (MQ=60)
                                                                                                 ATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGC                                                  >  SRR19973069.2121793/1‑150 (MQ=60)
                                                                                                  TCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCT                                                 <  SRR19973069.2532675/150‑1 (MQ=60)
                                                                                                  TCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCT                                                 <  SRR19973069.224610/150‑1 (MQ=60)
                                                                                                   CATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTC                                                >  SRR19973069.2036749/1‑150 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               >  SRR19973069.1906283/1‑150 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               <  SRR19973069.3598854/150‑1 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               <  SRR19973069.3598864/150‑1 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               <  SRR19973069.3599840/150‑1 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               <  SRR19973069.1694908/150‑1 (MQ=60)
                                                                                                    ATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCT                                               >  SRR19973069.2213415/1‑150 (MQ=60)
                                                                                                      CACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTGT                                             >  SRR19973069.3780018/1‑150 (MQ=60)
                                                                                                       ACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTC                                            <  SRR19973069.1060724/150‑1 (MQ=60)
                                                                                                       ACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTC                                            <  SRR19973069.1159743/150‑1 (MQ=60)
                                                                                                       ACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTC                                            <  SRR19973069.1159738/150‑1 (MQ=60)
                                                                                                       ACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTC                                            <  SRR19973069.2164410/150‑1 (MQ=60)
                                                                                                         TGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGT                                          <  SRR19973069.3649175/150‑1 (MQ=60)
                                                                                                                    CGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCA                               >  SRR19973069.3759569/1‑150 (MQ=60)
                                                                                                                       TGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCA                            >  SRR19973069.2092372/1‑150 (MQ=60)
                                                                                                                       TGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCA                            >  SRR19973069.2092344/1‑150 (MQ=60)
                                                                                                                       TGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCA                            <  SRR19973069.2798041/150‑1 (MQ=60)
                                                                                                                           CGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTTGCAATCACCGGAATATTAAGCCGCAGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCAAGCACCACCAG                        >  SRR19973069.3496535/1‑150 (MQ=60)
                                                                                                                               ATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCC                    >  SRR19973069.953540/1‑150 (MQ=60)
                                                                                                                                TGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCG                   <  SRR19973069.964073/150‑1 (MQ=60)
                                                                                                                                TGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCG                   <  SRR19973069.964046/150‑1 (MQ=60)
                                                                                                                                   ATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAG                >  SRR19973069.3566513/1‑150 (MQ=60)
                                                                                                                                   ATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAG                >  SRR19973069.3566448/1‑150 (MQ=60)
                                                                                                                                     TGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCG              <  SRR19973069.158429/150‑1 (MQ=60)
                                                                                                                                       TTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCC            <  SRR19973069.2543475/150‑1 (MQ=60)
                                                                                                                                            CGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCT       <  SRR19973069.3008920/150‑1 (MQ=60)
                                                                                                                                               TCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT    <  SRR19973069.2252104/150‑1 (MQ=60)
                                                                                                                                               TCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT    <  SRR19973069.2251778/150‑1 (MQ=60)
                                                                                                                                                 TGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGA  >  SRR19973069.1684025/1‑150 (MQ=60)
                                                                                                                                                 TGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGA  >  SRR19973069.2857472/1‑150 (MQ=60)
                                                                                                                                                 TGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGA  >  SRR19973069.3723340/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
TTTTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGA  >  CP009273/2223100‑2223394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑