Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,380 C→A 100% P368P (CCG→CCT)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,3800CA97.5% 626.6 / 6.2 199P368P (CCG→CCT) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (94/100);  total (96/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 199 total aligned reads displayed.

TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCA  >  CP009273/2699235‑2699521
                                                                                                                                                 |                                                                                                                                             
tGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAgg                                                                                                                                             >  1:3378337/1‑148 (MQ=255)
   cGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGgcgc                                                                                                                                          <  2:691849/148‑1 (MQ=255)
    gagaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCa                                                                                                                                         <  1:1529483/148‑1 (MQ=255)
     agaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg                                                                                                                                        >  1:3245145/1‑148 (MQ=255)
     agaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg                                                                                                                                        >  1:969335/1‑148 (MQ=255)
     agaTATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAg                                                                                                                                        >  2:1730263/1‑148 (MQ=255)
       atatGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtg                                                                                                                                      >  2:540272/1‑148 (MQ=255)
       atatGCCTGCGGCAGCAGCAGGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtg                                                                                                                                      >  2:538734/1‑148 (MQ=255)
         atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggt                                                                                                                                    <  1:2828014/148‑1 (MQ=255)
         atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggt                                                                                                                                    >  2:273812/1‑148 (MQ=255)
         atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggt                                                                                                                                    <  2:2835433/148‑1 (MQ=255)
          tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggtg                                                                                                                                   >  1:2880860/1‑148 (MQ=255)
           gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGa                                                                                                                                  <  2:3165203/148‑1 (MQ=255)
           gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGa                                                                                                                                  >  1:1336989/1‑148 (MQ=255)
             cTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATc                                                                                                                                <  2:345972/148‑1 (MQ=255)
             cTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATc                                                                                                                                <  2:1690915/148‑1 (MQ=255)
             cTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATc                                                                                                                                <  2:3770952/148‑1 (MQ=255)
                cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcaga                                                                                                                             <  1:3715816/148‑1 (MQ=255)
                   cagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatcc                                                                                                                          >  2:973091/1‑148 (MQ=255)
                     gcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTCGATGTTATTTACCGCAGGCAGCGTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccag                                                                                                                        >  2:628927/1‑148 (MQ=255)
                     gcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTGACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccag                                                                                                                        <  1:1608594/148‑1 (MQ=255)
                      cagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccaga                                                                                                                       <  2:3177467/148‑1 (MQ=255)
                       agcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccagat                                                                                                                      <  2:116776/148‑1 (MQ=255)
                          aTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGt                                                                                                                   <  2:1010600/148‑1 (MQ=255)
                           tgtgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTa                                                                                                                  >  2:1908154/1‑148 (MQ=255)
                           tgtgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTa                                                                                                                  >  1:3606050/1‑148 (MQ=255)
                            gtgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTAt                                                                                                                 <  2:2143759/148‑1 (MQ=255)
                                 acTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACg                                                                                                            >  1:898128/1‑148 (MQ=255)
                                    ctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTc                                                                                                         >  1:415680/1‑148 (MQ=255)
                                    ctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTc                                                                                                         >  1:303095/1‑148 (MQ=255)
                                    ctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTc                                                                                                         <  1:1981333/148‑1 (MQ=255)
                                      gCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCa                                                                                                       >  2:1067520/1‑148 (MQ=255)
                                          tCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACg                                                                                                   >  1:3539087/1‑148 (MQ=255)
                                             gCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTc                                                                                                >  2:1991816/1‑148 (MQ=255)
                                              cTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc                                                                                               >  1:1895859/1‑148 (MQ=255)
                                                 gcgcAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTgg                                                                                            >  2:770248/1‑148 (MQ=255)
                                                    cAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatg                                                                                         >  2:2968953/1‑148 (MQ=255)
                                                     aGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatga                                                                                        <  2:1822778/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                       <  1:1986168/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                       <  2:427333/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                       <  1:1358/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                       <  1:3308854/148‑1 (MQ=255)
                                                        tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtct                                                                                     >  2:2772436/1‑148 (MQ=255)
                                                        tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtct                                                                                     <  1:2853075/148‑1 (MQ=255)
                                                        tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtct                                                                                     >  1:1007470/1‑148 (MQ=255)
                                                           tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCa                                                                                  >  1:1636820/1‑148 (MQ=255)
                                                           tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCa                                                                                  >  2:3222181/1‑148 (MQ=255)
                                                            aGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAt                                                                                 >  1:394941/1‑148 (MQ=255)
                                                             gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAtg                                                                                >  1:264715/1‑148 (MQ=255)
                                                             gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAtg                                                                                <  2:3070497/148‑1 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                         >  2:3630620/1‑148 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                         <  1:1101139/148‑1 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                         <  1:1310886/148‑1 (MQ=255)
                                                                        ccGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGcc                                                                     >  2:3482690/1‑148 (MQ=255)
                                                                        ccGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGcc                                                                     >  2:2526839/1‑148 (MQ=255)
                                                                         cGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCg                                                                    >  2:3710799/1‑148 (MQ=255)
                                                                         cGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCg                                                                    >  2:1819037/1‑148 (MQ=255)
                                                                          gcaggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGa                                                                   <  2:3754978/148‑1 (MQ=255)
                                                                           caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAg                                                                  <  1:214323/148‑1 (MQ=255)
                                                                             ggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGa                                                                <  2:136511/148‑1 (MQ=255)
                                                                              gcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGaa                                                               >  1:2496462/1‑148 (MQ=255)
                                                                               cagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAg                                                              <  1:564977/148‑1 (MQ=255)
                                                                               cagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAg                                                              <  1:2577764/148‑1 (MQ=255)
                                                                                  cTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGCAGCCg                                                           >  1:3513415/1‑148 (MQ=255)
                                                                                  cTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg                                                           >  2:3002049/1‑148 (MQ=255)
                                                                                   ttGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTTATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGc                                                          <  2:906801/148‑1 (MQ=255)
                                                                                     ggATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAg                                                        <  1:2399925/148‑1 (MQ=255)
                                                                                      gATGGGCTTACGACGTAGATAACTTATCTTGACGTGGTTTCAAATTCATACACTAAGGTAGGCGCAGTGGTGATCAGATCCAGAACGTATTCACCTTCCAGACGTACCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGc                                                       >  2:3480209/1‑148 (MQ=255)
                                                                                       aTGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                                                      >  1:2940209/1‑148 (MQ=255)
                                                                                          ggCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaa                                                   <  1:1487481/148‑1 (MQ=255)
                                                                                          ggCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaa                                                   <  1:2958687/148‑1 (MQ=255)
                                                                                                 gACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg                                            <  2:3378337/148‑1 (MQ=255)
                                                                                                    gTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGccc                                         <  2:2880860/148‑1 (MQ=255)
                                                                                                     tAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCa                                        <  1:2731525/148‑1 (MQ=255)
                                                                                                      aGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAg                                       >  2:41079/1‑148 (MQ=255)
                                                                                                        aTAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcg                                     >  1:457567/1‑148 (MQ=255)
                                                                                                           aCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgcgc                                  >  2:943778/1‑148 (MQ=255)
                                                                                                           aCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgcgc                                  <  1:3121200/148‑1 (MQ=255)
                                                                                                                 ctTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGc                            <  2:1282287/148‑1 (MQ=255)
                                                                                                                 ctTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGc                            <  1:3741447/148‑1 (MQ=255)
                                                                                                                  ttGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCt                           <  1:747160/148‑1 (MQ=255)
                                                                                                                     aCGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                        >  1:2733989/1‑148 (MQ=255)
                                                                                                                     aCGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                        <  2:3790632/148‑1 (MQ=255)
                                                                                                                        tGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCgg                     >  1:882682/1‑148 (MQ=255)
                                                                                                                        tGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCgg                     >  1:2796944/1‑148 (MQ=255)
                                                                                                                          gTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCt                   <  2:1483812/148‑1 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                 >  1:2091920/1‑148 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                 <  1:593086/148‑1 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                 <  1:71030/148‑1 (MQ=255)
                                                                                                                             tCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCAt                <  2:2193498/148‑1 (MQ=255)
                                                                                                                               aaCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAg              >  1:3475396/1‑148 (MQ=255)
                                                                                                                               aaCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAg              >  1:1129810/1‑148 (MQ=255)
                                                                                                                                aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGa             >  1:2785241/1‑148 (MQ=255)
                                                                                                                                aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGa             <  1:2365744/148‑1 (MQ=255)
                                                                                                                                aCTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGa             >  1:1066146/1‑148 (MQ=255)
                                                                                                                                 cTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa            >  2:512809/1‑148 (MQ=255)
                                                                                                                                   tCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa          <  2:748133/148‑1 (MQ=255)
                                                                                                                                    cATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAg         <  1:185160/148‑1 (MQ=255)
                                                                                                                                      tACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg       >  2:1133473/1‑148 (MQ=255)
                                                                                                                                      tACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg       <  2:898128/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCA  >  CP009273/2699235‑2699521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA  >  CP009273/2699233‑2699528
                                                                                                                                                   |                                                                                                                                                    
GTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGG                                                                                                                                                    >  SRR19973069.3400412/1‑150 (MQ=60)
     CGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAG                                                                                                                                               >  SRR19973069.975797/1‑150 (MQ=60)
     CGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAG                                                                                                                                               >  SRR19973069.3266386/1‑150 (MQ=60)
      GAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGT                                                                                                                                              <  SRR19973069.1539997/150‑1 (MQ=60)
        GATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGG                                                                                                                                            <  SRR19973069.1347696/150‑1 (MQ=60)
          TATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTG                                                                                                                                          >  SRR19973069.2899782/1‑150 (MQ=60)
           ATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGA                                                                                                                                         <  SRR19973069.2846708/150‑1 (MQ=60)
           ATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGA                                                                                                                                         >  SRR19973069.1346216/1‑150 (MQ=60)
              CCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCA                                                                                                                                      >  SRR19973069.3112304/1‑150 (MQ=60)
               CTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAG                                                                                                                                     <  SRR19973069.2629068/150‑1 (MQ=60)
                  CGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATC                                                                                                                                  <  SRR19973069.2506442/150‑1 (MQ=60)
                  CGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATC                                                                                                                                  <  SRR19973069.3740079/150‑1 (MQ=60)
                       GCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTGACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGAT                                                                                                                             <  SRR19973069.1619409/150‑1 (MQ=60)
                        CAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATC                                                                                                                            <  SRR19973069.2080379/150‑1 (MQ=60)
                         AGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCG                                                                                                                           >  SRR19973069.3359971/1‑150 (MQ=60)
                         AGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCG                                                                                                                           >  SRR19973069.1639562/1‑150 (MQ=60)
                           CATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTA                                                                                                                         >  SRR19973069.3629594/1‑150 (MQ=60)
                              GTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTC                                                                                                                      <  SRR19973069.1593053/150‑1 (MQ=60)
                                GACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTTTTTACCGCAGGCAGGTTGGATGTGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCAC                                                                                                                    <  SRR19973069.1921546/150‑1 (MQ=60)
                                 ACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACG                                                                                                                   >  SRR19973069.904311/1‑150 (MQ=60)
                                  CACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGT                                                                                                                  >  SRR19973069.1852044/1‑150 (MQ=60)
                                    CTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTC                                                                                                                >  SRR19973069.418729/1‑150 (MQ=60)
                                    CTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTC                                                                                                                >  SRR19973069.305144/1‑150 (MQ=60)
                                     TCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCC                                                                                                               <  SRR19973069.321346/150‑1 (MQ=60)
                                      CTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCA                                                                                                              >  SRR19973069.2186209/1‑150 (MQ=60)
                                      CTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCA                                                                                                              >  SRR19973069.2778132/1‑150 (MQ=60)
                                      CTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCA                                                                                                              <  SRR19973069.1995109/150‑1 (MQ=60)
                                      CTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCA                                                                                                              <  SRR19973069.452243/150‑1 (MQ=60)
                                          AATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACG                                                                                                          >  SRR19973069.3562045/1‑150 (MQ=60)
                                          AATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACG                                                                                                          >  SRR19973069.3561003/1‑150 (MQ=60)
                                              GGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCC                                                                                                      >  SRR19973069.1908986/1‑150 (MQ=60)
                                              GGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCC                                                                                                      >  SRR19973069.1526314/1‑150 (MQ=60)
                                                        GTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCT                                                                                            <  SRR19973069.3330531/150‑1 (MQ=60)
                                                        GTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCT                                                                                            >  SRR19973069.1014403/1‑150 (MQ=60)
                                                        GTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCT                                                                                            <  SRR19973069.1999997/150‑1 (MQ=60)
                                                        GTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCT                                                                                            <  SRR19973069.1999947/150‑1 (MQ=60)
                                                        GTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCT                                                                                            <  SRR19973069.1383/150‑1 (MQ=60)
                                                          TCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCC                                                                                          <  SRR19973069.2871942/150‑1 (MQ=60)
                                                          TCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCC                                                                                          <  SRR19973069.2871856/150‑1 (MQ=60)
                                                          TCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCC                                                                                          <  SRR19973069.2871629/150‑1 (MQ=60)
                                                           CGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCA                                                                                         >  SRR19973069.1648034/1‑150 (MQ=60)
                                                            GTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAT                                                                                        >  SRR19973069.397843/1‑150 (MQ=60)
                                                            GTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAT                                                                                        >  SRR19973069.397847/1‑150 (MQ=60)
                                                             TAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATG                                                                                       >  SRR19973069.266611/1‑150 (MQ=60)
                                                                  GTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAG                                                                                  <  SRR19973069.547329/150‑1 (MQ=60)
                                                                  GTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGAGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAG                                                                                  >  SRR19973069.2550174/1‑150 (MQ=60)
                                                                     ATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAG                                                                               <  SRR19973069.96316/150‑1 (MQ=60)
                                                                      TTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGG                                                                              <  SRR19973069.1108834/150‑1 (MQ=60)
                                                                      TTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGG                                                                              <  SRR19973069.1108840/150‑1 (MQ=60)
                                                                      TTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGG                                                                              <  SRR19973069.1319852/150‑1 (MQ=60)
                                                                      TTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGG                                                                              <  SRR19973069.1320312/150‑1 (MQ=60)
                                                                             CAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGA                                                                       <  SRR19973069.216007/150‑1 (MQ=60)
                                                                              AGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAA                                                                      >  SRR19973069.2513150/1‑150 (MQ=60)
                                                                               GGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAG                                                                     <  SRR19973069.2834617/150‑1 (MQ=60)
                                                                                 CAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCC                                                                   <  SRR19973069.568908/150‑1 (MQ=60)
                                                                                 CAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCC                                                                   <  SRR19973069.2594883/150‑1 (MQ=60)
                                                                                  AGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGCAGCCG                                                                  >  SRR19973069.3536067/1‑150 (MQ=60)
                                                                                  AGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCG                                                                  >  SRR19973069.3535165/1‑150 (MQ=60)
                                                                                       GGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCG                                                             >  SRR19973069.2959707/1‑150 (MQ=60)
                                                                                       GGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCG                                                             >  SRR19973069.2959819/1‑150 (MQ=60)
                                                                                       GGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCG                                                             <  SRR19973069.2416175/150‑1 (MQ=60)
                                                                                       GGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCG                                                             <  SRR19973069.2416151/150‑1 (MQ=60)
                                                                                         ATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGA                                                           >  SRR19973069.3284294/1‑150 (MQ=60)
                                                                                            GGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAC                                                        <  SRR19973069.1497816/150‑1 (MQ=60)
                                                                                            GGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAC                                                        <  SRR19973069.2978318/150‑1 (MQ=60)
                                                                                                  CGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC                                                  <  SRR19973069.732731/150‑1 (MQ=60)
                                                                                                      GTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGCATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAG                                              >  SRR19973069.3690914/1‑150 (MQ=60)
                                                                                                       TAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGC                                             <  SRR19973069.2749811/150‑1 (MQ=60)
                                                                                                       TAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGC                                             <  SRR19973069.2749836/150‑1 (MQ=60)
                                                                                                        AGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCG                                            >  SRR19973069.3622455/1‑150 (MQ=60)
                                                                                                        AGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCG                                            >  SRR19973069.460919/1‑150 (MQ=60)
                                                                                                             ACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTG                                       >  SRR19973069.1083637/1‑150 (MQ=60)
                                                                                                             ACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTG                                       <  SRR19973069.3141700/150‑1 (MQ=60)
                                                                                                              CTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGG                                      >  SRR19973069.90120/1‑150 (MQ=60)
                                                                                                              CTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGG                                      >  SRR19973069.2700618/1‑150 (MQ=60)
                                                                                                               TTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGA                                     <  SRR19973069.299757/150‑1 (MQ=60)
                                                                                                                   CTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTT                                 <  SRR19973069.3765829/150‑1 (MQ=60)
                                                                                                                   CTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTT                                 <  SRR19973069.3765615/150‑1 (MQ=60)
                                                                                                                    TTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTT                                <  SRR19973069.752453/150‑1 (MQ=60)
                                                                                                                     TGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTC                               >  SRR19973069.2752285/1‑150 (MQ=60)
                                                                                                                       ACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCGGGAGCTTTCCG                             <  SRR19973069.2942956/150‑1 (MQ=60)
                                                                                                                        CGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGG                            >  SRR19973069.2815527/1‑150 (MQ=60)
                                                                                                                        CGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGG                            >  SRR19973069.888795/1‑150 (MQ=60)
                                                                                                                        CGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGG                            <  SRR19973069.1906055/150‑1 (MQ=60)
                                                                                                                            GTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCA                        >  SRR19973069.2106385/1‑150 (MQ=60)
                                                                                                                              TTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATA                      <  SRR19973069.71840/150‑1 (MQ=60)
                                                                                                                              TTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATA                      <  SRR19973069.597115/150‑1 (MQ=60)
                                                                                                                              TTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATA                      <  SRR19973069.597052/150‑1 (MQ=60)
                                                                                                                               TCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAG                     >  SRR19973069.3497860/1‑150 (MQ=60)
                                                                                                                               TCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAG                     >  SRR19973069.1137689/1‑150 (MQ=60)
                                                                                                                               TCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAG                     >  SRR19973069.3497738/1‑150 (MQ=60)
                                                                                                                                CAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGA                    >  SRR19973069.1073574/1‑150 (MQ=60)
                                                                                                                                CAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGA                    >  SRR19973069.2803776/1‑150 (MQ=60)
                                                                                                                                CAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGA                    >  SRR19973069.2803814/1‑150 (MQ=60)
                                                                                                                                  ACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAC                  <  SRR19973069.1416638/150‑1 (MQ=60)
                                                                                                                                  ACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAC                  <  SRR19973069.2381811/150‑1 (MQ=60)
                                                                                                                                  ACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAC                  >  SRR19973069.1155951/1‑150 (MQ=60)
                                                                                                                                      CATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTG              <  SRR19973069.186692/150‑1 (MQ=60)
                                                                                                                                      CATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTG              >  SRR19973069.711437/1‑150 (MQ=60)
                                                                                                                                         ACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGG           >  SRR19973069.2153337/1‑150 (MQ=60)
                                                                                                                                          CACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGC          >  SRR19973069.759293/1‑150 (MQ=60)
                                                                                                                                            CTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAT        <  SRR19973069.3788864/150‑1 (MQ=60)
                                                                                                                                                GGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTT    >  SRR19973069.2796343/1‑150 (MQ=60)
                                                                                                                                                  TAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA  <  SRR19973069.322884/150‑1 (MQ=60)
                                                                                                                                                  TAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA  <  SRR19973069.3145479/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA  >  CP009273/2699233‑2699528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑