Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,831,929 T→A 100% E232V (GAA→GTA)  ascG ← asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,831,9290TA99.0% 706.0 / ‑2.5 215E232V (GAA→GTA) ascGasc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (95/117);  total (95/120)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

GCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGA  >  CP009273/2831782‑2832073
                                                                                                                                                   |                                                                                                                                                
gCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACAttt                                                                                                                                                  <  2:2565591/148‑1 (MQ=255)
   aTAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTa                                                                                                                                               <  1:2654996/148‑1 (MQ=255)
   aTAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTa                                                                                                                                               <  1:675939/148‑1 (MQ=255)
   aTAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGAGCTCGTGTAACTCTTTCATCGCACCTATCTCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTa                                                                                                                                               <  1:2651227/148‑1 (MQ=255)
    tAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATtactac                                                                                                                                              <  1:3272990/148‑1 (MQ=255)
    tAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATtactac                                                                                                                                              >  1:608904/1‑148 (MQ=255)
     aGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTAcc                                                                                                                                             >  1:338754/1‑148 (MQ=255)
      gCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTAccc                                                                                                                                            <  1:1615012/148‑1 (MQ=255)
      gCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTAccc                                                                                                                                            >  1:129262/1‑148 (MQ=255)
        gATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCt                                                                                                                                          <  1:2992895/148‑1 (MQ=255)
        gATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCt                                                                                                                                          <  1:923140/148‑1 (MQ=255)
          tatCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTc                                                                                                                                        >  1:1832622/1‑148 (MQ=255)
          tatCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTAATGGCAACTAACGCGCAAAATTTAGCCCCACGTTCGAGCAACAATACTACCCCATc                                                                                                                                        >  1:1350512/1‑148 (MQ=255)
           atcatcGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCg                                                                                                                                       >  1:931690/1‑148 (MQ=255)
            tcatcGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCGAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCgg                                                                                                                                      >  1:3656809/1‑148 (MQ=255)
                 gAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTTACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCg                                                                                                                                 <  2:3755810/148‑1 (MQ=255)
                     ccGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTgg                                                                                                                             >  1:3406559/1‑148 (MQ=255)
                        aTAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAg                                                                                                                          >  1:2552583/1‑148 (MQ=255)
                            cTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGt                                                                                                                      <  1:1585728/148‑1 (MQ=255)
                               acacCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCa                                                                                                                   >  1:3187031/1‑148 (MQ=255)
                               acacCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCa                                                                                                                   <  2:3050405/148‑1 (MQ=255)
                               acacCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCa                                                                                                                   <  1:418527/148‑1 (MQ=255)
                                cacCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCAt                                                                                                                  <  2:920150/148‑1 (MQ=255)
                                cacCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCAt                                                                                                                  <  2:3267555/148‑1 (MQ=255)
                                  ccTGCTCTGGCACCGCTACGACGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACAGGCAACAAACGCGCGAAAATTATACCCACCTTAGAGCAACATTAATACCCCTTCGGCCCCGCTGGCAGGCGTCCAttt                                                                                                                >  1:1471101/1‑148 (MQ=255)
                                   cTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTa                                                                                                               <  2:2078732/148‑1 (MQ=255)
                                     gCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTAcc                                                                                                             <  2:1380404/148‑1 (MQ=255)
                                     gCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTAcc                                                                                                             <  2:281260/148‑1 (MQ=255)
                                     gCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTAcc                                                                                                             >  2:3353930/1‑148 (MQ=255)
                                            cACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCg                                                                                                      <  1:2633493/148‑1 (MQ=255)
                                            cACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCg                                                                                                      <  1:865159/148‑1 (MQ=255)
                                             aCCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACAACCCCGTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGa                                                                                                     >  1:1907957/1‑148 (MQ=255)
                                              ccGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCCTTTACCGTTAGCGAt                                                                                                    >  1:56314/1‑148 (MQ=255)
                                               cGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATa                                                                                                   <  2:3799315/148‑1 (MQ=255)
                                                gCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCAGATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATaa                                                                                                  <  1:3137609/148‑1 (MQ=255)
                                                 cTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCGATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAg                                                                                                 >  1:1404876/1‑148 (MQ=255)
                                                 cTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAg                                                                                                 >  2:1075047/1‑148 (MQ=255)
                                                 cTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAg                                                                                                 >  1:1320557/1‑148 (MQ=255)
                                                 cTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAg                                                                                                 >  1:1404777/1‑148 (MQ=255)
                                                 cTACGCCGCGCTCGTGAAACGATTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTATCCCCACGTTCGAGCAACAATACTACCCCTACTGCCCCGCTCGCAGGCGTACATTTAACGTTATCGAAAAg                                                                                                 >  2:957405/1‑148 (MQ=255)
                                                  tACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGt                                                                                                <  2:3453191/148‑1 (MQ=255)
                                                    cgccgcGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGttt                                                                                              >  2:2014386/1‑148 (MQ=255)
                                                     gccgcGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGtttt                                                                                             <  1:3339461/148‑1 (MQ=255)
                                                     gccgcGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGtttt                                                                                             <  1:1899533/148‑1 (MQ=255)
                                                        gcgcTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCa                                                                                          <  1:3554425/148‑1 (MQ=255)
                                                         cgcTCGTGTAACTGTTTCTTCGGAGCTATCGGGTTATCGTCGTTTCTGTGAACTTACGCGCTATATTTAGGGCCAGTTTCGAGCAACATTTCTACCGCTTCGTCGCCGCTGGGTGGCGTCCTTTTTACGTTAGCGTTTAGTTTTTctt                                                                                         <  2:2927795/148‑3 (MQ=255)
                                                         cgcTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCAt                                                                                         <  1:2052002/148‑1 (MQ=255)
                                                         cgcTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCAt                                                                                         <  2:3064359/148‑1 (MQ=255)
                                                           cTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTg                                                                                       <  2:2121969/148‑1 (MQ=255)
                                                               tgtAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAgcg                                                                                   <  2:2330307/148‑1 (MQ=255)
                                                               tgtAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAgcg                                                                                   <  1:675275/148‑1 (MQ=255)
                                                                 tAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCa                                                                                 <  1:1091262/148‑1 (MQ=255)
                                                                 tAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCa                                                                                 <  1:1968506/148‑1 (MQ=255)
                                                                  aaCGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCaa                                                                                >  2:81899/1‑148 (MQ=255)
                                                                      cTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATAcc                                                                            <  2:1689765/148‑1 (MQ=255)
                                                                        ttCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAt                                                                          >  1:771237/1‑148 (MQ=255)
                                                                          cATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAtgc                                                                        <  2:3510552/148‑1 (MQ=255)
                                                                           aTCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAtgct                                                                       >  2:3436811/1‑148 (MQ=255)
                                                                             cGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAtgctgc                                                                     >  1:1254111/1‑148 (MQ=255)
                                                                              gCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTgcg                                                                    <  2:1429466/148‑1 (MQ=255)
                                                                              gCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTgcg                                                                    >  2:1643011/1‑148 (MQ=255)
                                                                                        ccATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTc                                                          <  2:516898/148‑1 (MQ=255)
                                                                                        ccATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTc                                                          <  2:2433661/148‑1 (MQ=255)
                                                                                        ccATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTc                                                          >  2:3651604/1‑148 (MQ=255)
                                                                                           tatCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCACTACCATGCTGCGCCAGCGCGTCttt                                                       >  2:1034195/1‑148 (MQ=255)
                                                                                           tatCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCttt                                                       >  2:1485358/1‑148 (MQ=255)
                                                                                            atCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTta                                                      <  2:177148/148‑1 (MQ=255)
                                                                                             tcgtcgTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTtat                                                     <  2:1742892/148‑1 (MQ=255)
                                                                                             tcgtcgTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTtat                                                     <  2:578276/148‑1 (MQ=255)
                                                                                                tcgtTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATAcc                                                  <  1:3752019/148‑1 (MQ=255)
                                                                                                   ttACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCgg                                               <  2:1126448/148‑1 (MQ=255)
                                                                                                    tACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGc                                              >  2:891211/1‑148 (MQ=255)
                                                                                                       tGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAg                                           <  1:68624/148‑1 (MQ=255)
                                                                                                          cAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACg                                        >  1:3106441/1‑148 (MQ=255)
                                                                                                            aCTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGtt                                      >  1:2304846/1‑148 (MQ=255)
                                                                                                            aCTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGtt                                      <  1:2578194/148‑1 (MQ=255)
                                                                                                            aCTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGtt                                      <  1:1506221/148‑1 (MQ=255)
                                                                                                              tAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCa                                    <  1:2865883/148‑1 (MQ=255)
                                                                                                               aaCGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCaa                                   <  1:3771110/148‑1 (MQ=255)
                                                                                                                 cgcgCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATg                                 <  1:538561/148‑1 (MQ=255)
                                                                                                                   cgcTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCt                               >  1:758380/1‑148 (MQ=255)
                                                                                                                      tAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggt                            <  1:1772799/148‑1 (MQ=255)
                                                                                                                        aaTTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggtgg                          >  2:1306346/1‑148 (MQ=255)
                                                                                                                        aaTTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggtgg                          >  2:2716413/1‑148 (MQ=255)
                                                                                                                        aaTTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggtgg                          <  2:2965002/148‑1 (MQ=255)
                                                                                                                          tttAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTgggg                        >  2:1501825/1‑148 (MQ=255)
                                                                                                                           ttAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTggggg                       <  2:3141542/148‑1 (MQ=255)
                                                                                                                              gCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCCGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAAt                    >  2:2197686/1‑148 (MQ=255)
                                                                                                                              gCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAAt                    <  1:17937/148‑1 (MQ=255)
                                                                                                                                cccACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATcc                  <  2:3593021/148‑1 (MQ=255)
                                                                                                                                 ccACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCa                 <  1:2880715/148‑1 (MQ=255)
                                                                                                                                  cACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCAt                >  2:1943/1‑148 (MQ=255)
                                                                                                                                  cACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCAt                <  1:2996702/148‑1 (MQ=255)
                                                                                                                                    cGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAAAACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCg              >  2:431828/1‑148 (MQ=255)
                                                                                                                                      ttCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAg            <  2:2343035/148‑1 (MQ=255)
                                                                                                                                      ttCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAg            >  2:2189320/1‑148 (MQ=255)
                                                                                                                                      ttCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAg            >  1:1042643/1‑148 (MQ=255)
                                                                                                                                       tCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGc           >  1:1703358/1‑148 (MQ=255)
                                                                                                                                             aaCATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTaa     <  2:2737643/148‑1 (MQ=255)
                                                                                                                                               cATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAgg   >  2:2650930/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGA  >  CP009273/2831782‑2832073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAA  >  CP009273/2831780‑2832075
                                                                                                                                                     |                                                                                                                                                  
GGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTA                                                                                                                                                    <  SRR19973069.137636/150‑1 (MQ=60)
  GCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACT                                                                                                                                                  >  SRR19973069.1449063/1‑150 (MQ=60)
    AATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTAC                                                                                                                                                >  SRR19973069.612986/1‑150 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               <  SRR19973069.2669007/150‑1 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               <  SRR19973069.2672789/150‑1 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               <  SRR19973069.680608/150‑1 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               >  SRR19973069.341133/1‑150 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               >  SRR19973069.1713943/1‑150 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               >  SRR19973069.341128/1‑150 (MQ=60)
     ATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGAGCTCGTGTAACTCTTTCATCGCACCTATCTCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACC                                                                                                                                               <  SRR19973069.2668989/150‑1 (MQ=60)
      TAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCC                                                                                                                                              <  SRR19973069.3294420/150‑1 (MQ=60)
      TAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCC                                                                                                                                              >  SRR19973069.130472/1‑150 (MQ=60)
        GCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCT                                                                                                                                            <  SRR19973069.1625904/150‑1 (MQ=60)
        GCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCT                                                                                                                                            <  SRR19973069.1625897/150‑1 (MQ=60)
        GCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCT                                                                                                                                            <  SRR19973069.1625808/150‑1 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          >  SRR19973069.1359867/1‑150 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          >  SRR19973069.3248780/1‑150 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          >  SRR19973069.1845462/1‑150 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          <  SRR19973069.929453/150‑1 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          <  SRR19973069.929426/150‑1 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTC                                                                                                                                          <  SRR19973069.3012767/150‑1 (MQ=60)
          GATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTAATGGCAACTAACGCGCAAAATTTAGCCCCACGTTCGAGCAACAATACTACCCCATC                                                                                                                                          >  SRR19973069.1359866/1‑150 (MQ=60)
           ATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCACCATTACTACCCCTTCG                                                                                                                                         >  SRR19973069.2663677/1‑150 (MQ=60)
           ATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCG                                                                                                                                         >  SRR19973069.2984680/1‑150 (MQ=60)
           ATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCG                                                                                                                                         >  SRR19973069.3295839/1‑150 (MQ=60)
           ATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCG                                                                                                                                         >  SRR19973069.938009/1‑150 (MQ=60)
            TATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCGAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGG                                                                                                                                        >  SRR19973069.3680756/1‑150 (MQ=60)
              TCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCC                                                                                                                                      <  SRR19973069.2617093/150‑1 (MQ=60)
                   GAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCT                                                                                                                                 >  SRR19973069.2358966/1‑150 (MQ=60)
                    AATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTG                                                                                                                                <  SRR19973069.621719/150‑1 (MQ=60)
                     ATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGG                                                                                                                               >  SRR19973069.3428770/1‑150 (MQ=60)
                        CGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAG                                                                                                                            >  SRR19973069.2569439/1‑150 (MQ=60)
                              CTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCC                                                                                                                      >  SRR19973069.3188289/1‑150 (MQ=60)
                              CTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCC                                                                                                                      <  SRR19973069.1596471/150‑1 (MQ=60)
                               TGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCA                                                                                                                     >  SRR19973069.3207734/1‑150 (MQ=60)
                                 ACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATT                                                                                                                   <  SRR19973069.421595/150‑1 (MQ=60)
                                  CACCTGCTCTGGCACCGCTACGACGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACAGGCAACAAACGCGCGAAAATTATACCCACCTTAGAGCAACATTAATACCCCTTCGGCCCCGCTGGCAGGCGTCCATTT                                                                                                                  >  SRR19973069.1481355/1‑150 (MQ=60)
                                      TGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCG                                                                                                              <  SRR19973069.864517/150‑1 (MQ=60)
                                             GCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGA                                                                                                       >  SRR19973069.1921266/1‑150 (MQ=60)
                                             GCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACAACCCCGTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGA                                                                                                       >  SRR19973069.1921133/1‑150 (MQ=60)
                                              CACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCCTTTACCGTTAGCGAT                                                                                                      >  SRR19973069.57002/1‑150 (MQ=60)
                                              CACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGAT                                                                                                      <  SRR19973069.2651132/150‑1 (MQ=60)
                                              CACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGAT                                                                                                      <  SRR19973069.871202/150‑1 (MQ=60)
                                                 CGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCGATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAG                                                                                                   >  SRR19973069.1414676/1‑150 (MQ=60)
                                                 CGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAG                                                                                                   >  SRR19973069.1414521/1‑150 (MQ=60)
                                                 CGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAG                                                                                                   >  SRR19973069.1414577/1‑150 (MQ=60)
                                                 CGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAG                                                                                                   >  SRR19973069.1329619/1‑150 (MQ=60)
                                                 CGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAG                                                                                                   >  SRR19973069.1414592/1‑150 (MQ=60)
                                                  GCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCAGATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGT                                                                                                  <  SRR19973069.3158164/150‑1 (MQ=60)
                                                       GCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTC                                                                                             <  SRR19973069.3361326/150‑1 (MQ=60)
                                                       GCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTC                                                                                             <  SRR19973069.1914092/150‑1 (MQ=60)
                                                       GCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTC                                                                                             <  SRR19973069.1912675/150‑1 (MQ=60)
                                                        CCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCA                                                                                            >  SRR19973069.3616465/1‑150 (MQ=60)
                                                         CGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCAT                                                                                           <  SRR19973069.2883351/150‑1 (MQ=60)
                                                          GCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATT                                                                                          <  SRR19973069.3577540/150‑1 (MQ=60)
                                                          GCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATT                                                                                          <  SRR19973069.647666/150‑1 (MQ=60)
                                                           CGCTCGTGTTACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTG                                                                                         <  SRR19973069.920759/150‑1 (MQ=60)
                                                           CGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTG                                                                                         <  SRR19973069.135/150‑1 (MQ=60)
                                                           CGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTG                                                                                         <  SRR19973069.2615681/150‑1 (MQ=60)
                                                           CGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTG                                                                                         <  SRR19973069.2066245/150‑1 (MQ=60)
                                                             CTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACAGTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAG                                                                                       <  SRR19973069.559968/150‑1 (MQ=60)
                                                               CGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCG                                                                                     >  SRR19973069.1857946/1‑150 (MQ=60)
                                                                 TGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCCCGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCA                                                                                   <  SRR19973069.680128/150‑1 (MQ=60)
                                                                 TGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCA                                                                                   <  SRR19973069.679936/150‑1 (MQ=60)
                                                                   TAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAAT                                                                                 <  SRR19973069.1982172/150‑1 (MQ=60)
                                                                   TAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAAT                                                                                 <  SRR19973069.1098883/150‑1 (MQ=60)
                                                                   TAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAAT                                                                                 <  SRR19973069.132625/150‑1 (MQ=60)
                                                                    AACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGGTAGCGATAAGTTTTTCATTGAGCGCAATA                                                                                >  SRR19973069.1808226/1‑150 (MQ=60)
                                                                      CGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTAATTGAGCGCAATACC                                                                              >  SRR19973069.2230562/1‑150 (MQ=60)
                                                                        CTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAT                                                                            >  SRR19973069.776699/1‑150 (MQ=60)
                                                                        CTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAT                                                                            >  SRR19973069.777852/1‑150 (MQ=60)
                                                                        CTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCAT                                                                            <  SRR19973069.2889931/150‑1 (MQ=60)
                                                                           TCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCT                                                                         <  SRR19973069.1921038/150‑1 (MQ=60)
                                                                           TCATCGCACCTATCGCCATATCGTCGTTACAGGCAACTAACCCGCGAAATTTAGCCCCACGTTAGAGCAACATAACAAACCCATCGGCCCCCCTGGCAGGCGTCCATTTACAGGTAGCGATAAGATTTTAAATGAGCGCAAAACCATGCT                                                                         >  SRR19973069.512502/1‑150 (MQ=60)
                                                                             ATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGC                                                                       >  SRR19973069.1262588/1‑150 (MQ=60)
                                                                                GCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCC                                                                    <  SRR19973069.1512754/150‑1 (MQ=60)
                                                                                 CACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTTAGCGCAATACCATGCTGCGCCA                                                                   >  SRR19973069.397229/1‑150 (MQ=60)
                                                                                          CCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTT                                                          <  SRR19973069.3741828/150‑1 (MQ=60)
                                                                                           CATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTT                                                         <  SRR19973069.3792136/150‑1 (MQ=60)
                                                                                                  TCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCG                                                  <  SRR19973069.3776439/150‑1 (MQ=60)
                                                                                                    GTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGC                                                >  SRR19973069.1284123/1‑150 (MQ=60)
                                                                                                         TGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGAC                                           <  SRR19973069.69410/150‑1 (MQ=60)
                                                                                                         TGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGAC                                           <  SRR19973069.1052427/150‑1 (MQ=60)
                                                                                                          GGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACG                                          >  SRR19973069.3126940/1‑150 (MQ=60)
                                                                                                          GGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACG                                          >  SRR19973069.3126879/1‑150 (MQ=60)
                                                                                                            CAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTT                                        >  SRR19973069.2320522/1‑150 (MQ=60)
                                                                                                              ACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCA                                      <  SRR19973069.1516653/150‑1 (MQ=60)
                                                                                                              ACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCA                                      <  SRR19973069.2595084/150‑1 (MQ=60)
                                                                                                              ACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCA                                      <  SRR19973069.2595317/150‑1 (MQ=60)
                                                                                                                TAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAAT                                    <  SRR19973069.691594/150‑1 (MQ=60)
                                                                                                                TAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAAT                                    <  SRR19973069.690436/150‑1 (MQ=60)
                                                                                                                TAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAAT                                    <  SRR19973069.2884715/150‑1 (MQ=60)
                                                                                                                 AACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATG                                   <  SRR19973069.3795605/150‑1 (MQ=60)
                                                                                                                   CGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCT                                 <  SRR19973069.542358/150‑1 (MQ=60)
                                                                                                                   CGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCT                                 >  SRR19973069.764327/1‑150 (MQ=60)
                                                                                                                   CGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCT                                 >  SRR19973069.763733/1‑150 (MQ=60)
                                                                                                                       CTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTG                             <  SRR19973069.572190/150‑1 (MQ=60)
                                                                                                                        TAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGG                            <  SRR19973069.1785289/150‑1 (MQ=60)
                                                                                                                              TAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAAT                      <  SRR19973069.3055857/150‑1 (MQ=60)
                                                                                                                                GCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCC                    <  SRR19973069.18191/150‑1 (MQ=60)
                                                                                                                                GCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCC                    <  SRR19973069.1529024/150‑1 (MQ=60)
                                                                                                                                  CCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCAT                  <  SRR19973069.3326857/150‑1 (MQ=60)
                                                                                                                                   CCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATC                 <  SRR19973069.177564/150‑1 (MQ=60)
                                                                                                                                   CCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATC                 <  SRR19973069.179766/150‑1 (MQ=60)
                                                                                                                                   CCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATC                 >  SRR19973069.3393325/1‑150 (MQ=60)
                                                                                                                                   CCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATC                 <  SRR19973069.2899633/150‑1 (MQ=60)
                                                                                                                                    CACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCG                <  SRR19973069.3016596/150‑1 (MQ=60)
                                                                                                                                    CACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGGTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCG                <  SRR19973069.3014760/150‑1 (MQ=60)
                                                                                                                                    CACGGTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCG                <  SRR19973069.2942392/150‑1 (MQ=60)
                                                                                                                                      CGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAG              >  SRR19973069.1049888/1‑150 (MQ=60)
                                                                                                                                       GTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGC             >  SRR19973069.1715276/1‑150 (MQ=60)
                                                                                                                                          CGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGG          <  SRR19973069.3526736/150‑1 (MQ=60)
                                                                                                                                          CGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGG          <  SRR19973069.1226834/150‑1 (MQ=60)
                                                                                                                                            AGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTA        <  SRR19973069.3724238/150‑1 (MQ=60)
                                                                                                                                              CAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAG      >  SRR19973069.2022805/1‑150 (MQ=60)
                                                                                                                                                  ATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAA  >  SRR19973069.1551844/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAA  >  CP009273/2831780‑2832075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑