Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,299,240 A→C 100% intergenic (‑64/+90) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,299,2400AC99.2% 930.0 / ‑4.1 232intergenic (‑64/+90)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (110/120);  total (111/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

CCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299100‑3299384
                                                                                                                                             |                                                                                                                                                
ccAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTAc                                                                                                                                           >  1:782979/1‑148 (MQ=255)
  aataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaa                                                                                                                                         >  2:1979591/1‑148 (MQ=255)
  aataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGGTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTGGTTATGCATTG‑CACTGTACCAGTACCCGAGTCCaa                                                                                                                                         >  2:2160254/1‑148 (MQ=255)
   ataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaaa                                                                                                                                        <  1:3466769/148‑1 (MQ=255)
   ataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaaa                                                                                                                                        <  1:2565860/148‑1 (MQ=255)
   ataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaaa                                                                                                                                        <  1:250676/148‑1 (MQ=255)
   ataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaaa                                                                                                                                        <  1:2180335/148‑1 (MQ=255)
    taatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACaaaa                                                                                                                                       <  1:620572/148‑1 (MQ=255)
    taatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACACGAGTACaaaa                                                                                                                                       >  2:2718928/1‑148 (MQ=255)
     aatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAg                                                                                                                                      <  1:879739/148‑1 (MQ=255)
      atGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGa                                                                                                                                     <  1:988106/148‑1 (MQ=255)
       tGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGAc                                                                                                                                    <  2:3405630/148‑1 (MQ=255)
       tGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGAc                                                                                                                                    <  2:3217798/148‑1 (MQ=255)
       tGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGAc                                                                                                                                    <  2:3036038/148‑1 (MQ=255)
                cgATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGaaaaaaa                                                                                                                           <  1:1032659/148‑1 (MQ=255)
                cgATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGaaaaaaa                                                                                                                           <  1:2099366/148‑1 (MQ=255)
                 gATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAg                                                                                                                          >  2:49581/1‑148 (MQ=255)
                  aTAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGc                                                                                                                         >  1:3740913/1‑148 (MQ=255)
                   tAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcc                                                                                                                        >  1:2015342/1‑148 (MQ=255)
                   tAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcc                                                                                                                        <  1:3357905/148‑1 (MQ=255)
                   tAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGAATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcc                                                                                                                        >  1:1940211/1‑148 (MQ=255)
                     aTCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcccc                                                                                                                      >  1:3181797/1‑148 (MQ=255)
                       aaccacGCCGCCAAGCTGCGACGGTGAGGTTTGGGTGGTTGTTAGTGTTGCCATGTGGGTTTCTCTCCATTTAAAAATTGTGCGAATGCGTTGTTTTGCATTG‑CACTGTACCAGTACCCGATTACAAATGACACAAAAAAAGCCCCGa                                                                                                                    <  2:737297/147‑1 (MQ=255)
                        accacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGAt                                                                                                                   >  1:2919342/1‑148 (MQ=255)
                          cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                 >  1:2258805/1‑148 (MQ=255)
                          cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTAAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                 >  2:2642255/1‑148 (MQ=255)
                          caagccgccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                 >  2:1206682/4‑148 (MQ=255)
                           acGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGt                                                                                                                >  1:241318/1‑148 (MQ=255)
                           acGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGt                                                                                                                <  2:3630667/148‑1 (MQ=255)
                               cgccACGCAGCGAGGGTGACGTTTGGGTGGTGGTTTGTGTTGCCTTGAGGGCTTCTCTCCAGTTAAAAATAGTGCGACTGCTTTGTTATGCATTC‑CACTGTACCAGTACCCGAGTACAAAAGACATAAAAAAAGCCCCGATGGTaaaa                                                                                                            <  1:1166021/148‑1 (MQ=255)
                               cgcaatgcagcGAGGGTGAGGTTTGGGTGGTGTTTAGTGTTGCCTTGTGGGCTTCTCTCCAGTGAAAAATAGTGCGAGTGCTTTTTTATGCATTG‑CACTTTACCAGTACCCGATTAAAAAAGACACAAAAAAAGCCCCGATGGTaaaa                                                                                                            <  1:3641834/142‑1 (MQ=255)
                                 ccacccagcGACGGTGCCGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGGGCGCCTGCGTTGTTATGCATTG‑CACTGTACCAGTCCCCGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAAt                                                                                                          <  2:1638565/143‑1 (MQ=255)
                                 ccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAAt                                                                                                          <  2:208384/148‑1 (MQ=255)
                                    agcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgg                                                                                                       <  2:895693/148‑1 (MQ=255)
                                     gcagcGACGTTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTACTCTCCATTGAAAAATAGTGCGACTGCGTTTTTTTGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACTGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                      <  2:3575248/148‑1 (MQ=255)
                                     gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                      >  2:2714173/1‑148 (MQ=255)
                                     gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                      >  2:876067/1‑148 (MQ=255)
                                     gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                      >  1:219788/1‑148 (MQ=255)
                                      cagcGAGGGTGAGGTTGGGGTGGTGTTTTGTGTTGCCATGCGGGCTTCTCTCCAGTTAAAATTTGTGCGAGTGGGTTTTTATGCTTGG‑CACTGTACACGTCCCCGAGAACAAAAGCCACAAAAAAAGCCCCGATGGTAAAAATCgggg                                                                                                     <  1:3267073/148‑1 (MQ=255)
                                         cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATATTGCTACAGCGTTGTTATTCATTG‑CACTGTACAAGTACCAGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATAGGGGCTg                                                                                                  >  1:1850855/1‑148 (MQ=255)
                                           aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                >  2:2662422/1‑148 (MQ=255)
                                               tGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata                                                                                            >  2:2004440/1‑148 (MQ=255)
                                                        ggtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACACAAGACAGAAAAAAAGCCCCGATGGTAAAACTCGGGGCTGTATATATTATTTTAc                                                                                   >  2:3525745/1‑148 (MQ=255)
                                                          tggtgttTAGTGTTGCCATGATGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                 <  2:2060400/148‑1 (MQ=255)
                                                           ggtggtTTGTGTTGCCATGAGGGCTTCTCACCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                <  1:2861975/148‑1 (MQ=255)
                                                           ggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                <  2:602418/148‑1 (MQ=255)
                                                            gttgtTTGTGTGGCCATGAGGGCTTATCTCCAGTGAAAAATTGTGCGACTGTGTTTTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAt                                                                               <  1:2334957/145‑1 (MQ=255)
                                                              ggtTAGTGTTGCCATGAGGGCTTCTCTCCATTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                             <  2:1099245/148‑1 (MQ=255)
                                                                tttgtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCTTTTTTATGCTTTG‑CAGTGTACCAGTACCCGATTAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                           <  1:1979591/145‑1 (MQ=255)
                                                                ttAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                           >  1:1489387/1‑148 (MQ=255)
                                                                gttgtgtTGCGTTGCGGGGTTCTCTCGAGTTAAAAATTGTGCGATTGGGTTGTTTTGCATTG‑CACTGTACCAGTCCCCGCGTCAAAAAGAACCAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                           <  1:2160254/145‑1 (MQ=255)
                                                                 tAGTGTTGCCTTGCGGGCTTCTCTCCATTTAAAAATTGTGCGACTGCGTTGTTATGAATGG‑AAGTGTACCAGTACCCGATAACAAAACACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt                                                                          <  1:2660313/148‑1 (MQ=255)
                                                                  aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGtt                                                                         <  2:3351079/148‑1 (MQ=255)
                                                                   gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTTTTATGCATTG‑CACTGTACCAGTACCCGAGTAAAAAAGACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTc                                                                        <  1:304300/148‑1 (MQ=255)
                                                                   gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTc                                                                        >  2:1774651/1‑148 (MQ=255)
                                                                     gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                      <  1:1554176/148‑1 (MQ=255)
                                                                     gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                      <  2:3714633/148‑1 (MQ=255)
                                                                     gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                      <  2:3714680/148‑1 (MQ=255)
                                                                       tGCCTTGAGGGTTTCTCTCCTTTTAAAAATTGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCATTACCCTAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTg                                                                    <  1:440782/148‑1 (MQ=255)
                                                                          cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTc                                                                 <  2:1483566/148‑1 (MQ=255)
                                                                          cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTc                                                                 >  1:3412251/1‑148 (MQ=255)
                                                                            tGAGGGCTTCTCCCCAGTTAAAAATAGTGCGAC‑GGGTTTTTTAGGATTGCCACTTTACCCGTCCCCGAGAACAACACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAg                                                               <  1:1682835/148‑1 (MQ=255)
                                                                               gggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCga                                                            >  2:1250504/1‑148 (MQ=255)
                                                                                  cTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                         >  1:2457720/1‑148 (MQ=255)
                                                                                  cTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                         <  2:3749522/148‑1 (MQ=255)
                                                                                   ttCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatgat                                                        <  2:1801053/148‑1 (MQ=255)
                                                                                    tctctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                       >  1:537375/1‑148 (MQ=255)
                                                                                    tctctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                       >  1:100390/1‑148 (MQ=255)
                                                                                     ctctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTa                                                      <  1:526876/148‑1 (MQ=255)
                                                                                     ctctcCAGTGAAAAATAGTGCGACGGCGTTTTTATGCTTTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTa                                                      <  1:1393438/148‑1 (MQ=255)
                                                                                       ctcCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATGG‑CACTGTACCAGTACCCGATAAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACg                                                    <  1:1435935/148‑1 (MQ=255)
                                                                                          cAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCac                                                 <  2:1361311/148‑2 (MQ=255)
                                                                                             tGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcac                                              >  2:3250489/1‑148 (MQ=255)
                                                                                              gAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                             >  2:3480088/1‑148 (MQ=255)
                                                                                                   aTAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGa                                        >  1:56649/1‑148 (MQ=255)
                                                                                                   aTAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGa                                        >  1:1223316/1‑148 (MQ=255)
                                                                                                    tAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGaa                                       >  2:1744051/1‑148 (MQ=255)
                                                                                                    tAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGaa                                       <  2:1611268/148‑1 (MQ=255)
                                                                                                       tGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                    >  1:2639635/1‑148 (MQ=255)
                                                                                                       tGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                    <  1:2718928/148‑1 (MQ=255)
                                                                                                        gCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacga                                   >  1:662821/1‑148 (MQ=255)
                                                                                                           aCTGCTTTTTTATGCATTG‑CACTGTACCAGTACCCTAGTAAAAAATACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacga                                <  2:2569931/148‑1 (MQ=255)
                                                                                                           aCTGCTTTGTTATGAATGG‑CATTGTACCAGTACCCGATTACAAAATACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTTTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGTTGATTACGCATCACCACCGAAacgacga                                <  2:653131/148‑1 (MQ=255)
                                                                                                           aCTGCGTTGTTATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacga                                <  2:3181797/148‑1 (MQ=255)
                                                                                                                 ttgttATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCg                          >  1:3060639/1‑148 (MQ=255)
                                                                                                                  ttttATGCATTG‑CACTTTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                         <  2:1938834/146‑1 (MQ=255)
                                                                                                                  tgttATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                         >  2:1574962/1‑148 (MQ=255)
                                                                                                                    ttATGCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTa                       >  1:165616/1‑148 (MQ=255)
                                                                                                                        gCATTG‑CACTGTACCCGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                   <  2:2423183/148‑1 (MQ=255)
                                                                                                                        gCATTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                   >  2:2184519/1‑148 (MQ=255)
                                                                                                                        gCATTG‑CACTATACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                   >  2:3779548/1‑148 (MQ=255)
                                                                                                                        gCATTG‑CACTATACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAGTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                   >  2:662803/1‑148 (MQ=255)
                                                                                                                          aTTG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatca                 <  2:2510294/148‑1 (MQ=255)
                                                                                                                            tG‑CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc               >  1:3131547/1‑148 (MQ=255)
                                                                                                                             tgaaCTGTACCAGAACCCGTGTAAAAACGACACAAAAAATGCCCAGATGGTAAAACTCGGGGATTTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCTTAACCACCGAAACGACGACGACCGGTAGAatcatc               <  1:2224929/145‑1 (MQ=255)
                                                                                                                               cACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCac             >  2:2881044/1‑148 (MQ=255)
                                                                                                                                aCTGTACCCGCCCCCGAGAACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacg            <  1:2714173/148‑1 (MQ=255)
                                                                                                                                 cTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacga           >  1:3645521/1‑148 (MQ=255)
                                                                                                                                        aGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAg    >  1:2018522/1‑148 (MQ=255)
                                                                                                                                          tACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgcg  >  2:2208655/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTG‑CACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299100‑3299384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299093‑3299384
                                                                                                                                                   |                                                                                                                                                
CCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCG                                                                                                                                                <  SRR19973069.584096/150‑1 (MQ=60)
     CGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTAC                                                                                                                                           >  SRR19973069.788532/1‑150 (MQ=60)
          ATAATGGTGCCGCCGATAATCCCCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.2582827/150‑1 (MQ=60)
          ATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.2195198/150‑1 (MQ=60)
          ATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.3489198/150‑1 (MQ=60)
          ATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.2195199/150‑1 (MQ=60)
          ATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.2195208/150‑1 (MQ=60)
          ATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAG                                                                                                                                      <  SRR19973069.252517/150‑1 (MQ=60)
           TAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGA                                                                                                                                     <  SRR19973069.624698/150‑1 (MQ=60)
            AATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGAC                                                                                                                                    <  SRR19973069.885840/150‑1 (MQ=60)
            AATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGAC                                                                                                                                    <  SRR19973069.885229/150‑1 (MQ=60)
            AATGGTGCCGCCGATAATCACCACGCCGCCAAGAAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCAATGTACCAGTACCCCAGTACAAAATAC                                                                                                                                    >  SRR19973069.1710437/1‑150 (MQ=60)
             TTGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACA                                                                                                                                   <  SRR19973069.994808/150‑1 (MQ=60)
             ATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACA                                                                                                                                   <  SRR19973069.2916429/150‑1 (MQ=60)
             ATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACA                                                                                                                                   <  SRR19973069.2518338/150‑1 (MQ=60)
             ATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACA                                                                                                                                   <  SRR19973069.3301554/150‑1 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         <  SRR19973069.2537160/150‑1 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         >  SRR19973069.3024714/1‑150 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         >  SRR19973069.3765294/1‑150 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         <  SRR19973069.2113865/150‑1 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         <  SRR19973069.1039807/150‑1 (MQ=60)
                       CGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGC                                                                                                                         <  SRR19973069.1039818/150‑1 (MQ=60)
                        GATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCANTGAAAAAGAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGACAAAAAGCC                                                                                                                        >  SRR19973069.1952177/1‑150 (MQ=60)
                        GATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCC                                                                                                                        >  SRR19973069.2029355/1‑150 (MQ=60)
                        GATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGAATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCC                                                                                                                        >  SRR19973069.1953566/1‑150 (MQ=60)
                          TAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCC                                                                                                                      <  SRR19973069.3379877/150‑1 (MQ=60)
                          TAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCC                                                                                                                      <  SRR19973069.607372/150‑1 (MQ=60)
                          TAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCC                                                                                                                      >  SRR19973069.1285715/1‑150 (MQ=60)
                          TAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCC                                                                                                                      >  SRR19973069.3202489/1‑150 (MQ=60)
                           AATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCG                                                                                                                     >  SRR19973069.2737115/1‑150 (MQ=60)
                             TCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGAT                                                                                                                   >  SRR19973069.2938668/1‑150 (MQ=60)
                               ACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAATCCCCGATGG                                                                                                                 >  SRR19973069.2274082/1‑150 (MQ=60)
                               ACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGG                                                                                                                 >  SRR19973069.2274078/1‑150 (MQ=60)
                                CCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGT                                                                                                                >  SRR19973069.243121/1‑150 (MQ=60)
                                      CGCCACGCAGCGAGGGTGACGTTTGGGTGGTGGTTTGTGTTGCCTTGAGGGCTTCTCTCCAGTTAAAAATAGTGCGACTGCTTTGTTATGCATTCCACTGTACCAGTACCCGAGTACAAAAGACATAAAAAAAGCCCCGATGGTAAAAAT                                                                                                          <  SRR19973069.1174123/150‑1 (MQ=60)
                                      CGCCAAGCAGCGAGGGTGAGGTTTGGGTGGTTTTTTGTTTTGCATTGAGGGCTTATCTCCAGTGAAAAATAGTGCGACTGCTTTTTTTTGCATTGCAGTTTACCATTACCCGTGTAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAAT                                                                                                          <  SRR19973069.3666130/150‑1 (MQ=45)
                                      CGCAATGCAGCGAGGGTGAGGTTTGGGTGGTGTTTAGTGTTGCCTTGTGGGCTTCTCTCCAGTGAAAAATAGTGCGAGTGCTTTTTTATGCATTGCACTTTACCAGTACCCGATTAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAAT                                                                                                          <  SRR19973069.3665691/150‑1 (MQ=60)
                                       cccacgccgcgaggttgagGTTTGGGTGGTGTTTTGTGTTGCCTTGTGGGCTTCTCTCCTGTTAAAATTTGTGCGAGTGGTTTTTTATGATTTGCACTGTACCATTACCCTAGTAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAATC                                                                                                         <  SRR19973069.69500/131‑1 (MQ=21)
                                       GCCAAGCAGCGAGGGTTACTTTTGGGTGGTGTTTTGTGTTGCAATGAGGGATTCTCTCCAGTTAAAAATAGTGCGACTGGTTTTTTTTGCATTGCACTGTACCAGTACCCGATTAAAAAAGAAACAAAAAAAGCCCCGATGGTAAAAATC                                                                                                         <  SRR19973069.2015627/150‑1 (MQ=60)
                                       GCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATC                                                                                                         >  SRR19973069.2119261/1‑150 (MQ=60)
                                       GCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATC                                                                                                         >  SRR19973069.2119267/1‑150 (MQ=60)
                                          AAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGG                                                                                                      >  SRR19973069.221496/1‑150 (MQ=60)
                                          AAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCCAAGGTAAAAATCGGG                                                                                                      >  SRR19973069.221522/1‑150 (MQ=60)
                                             CAGCGAGGGTGAGGTTGGGGTGGTGTTTTGTGTTGCCATGCGGGCTTCTCTCCAGTTAAAATTTGTGCGAGTGGGTTTTTATGCTTGGCACTGTACACGTCCCCGAGAACAAAAGCCACAAAAAAAGCCCCGATGGTAAAAATCGGGGCT                                                                                                   <  SRR19973069.3288472/150‑1 (MQ=31)
                                             CAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCT                                                                                                   >  SRR19973069.2586915/1‑150 (MQ=60)
                                              AGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATATTGCTACAGCGTTGTTATTCATTGCACTGTACAAGTACCAGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATAGGGGCTG                                                                                                  >  SRR19973069.1863779/1‑150 (MQ=60)
                                              AGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTG                                                                                                  >  SRR19973069.360847/1‑150 (MQ=60)
                                                CGAGGGTGACGTTTGGGTGGTGTTTAGTGTTGCCATGTGGGCTTCTCTCAAGTGAAAAATAGTGCGACTGCTTTGTTATGCTTTGCACTGTACCAGTACCCGAGTACAAAACAAACAAAAAACGCCCCGATGGTAAAAATCGGGGCTGTA                                                                                                <  SRR19973069.3759074/150‑1 (MQ=60)
                                                 GACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTAT                                                                                               >  SRR19973069.3168462/1‑150 (MQ=60)
                                                   CGGTGACGTTTGGGTGGTGGTTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTCCCCGAGAACAAACCACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATAT                                                                                             <  SRR19973069.559391/150‑1 (MQ=60)
                                                         CGTTGGGGTGGTGGTTAGTTTTGCCATGAGGGCTTCTCTCCAGTCAAAAATAGTGCGACGGCGTTGTTTTGCATGGCACTGTACCAGAACCCGAGTACAAACCACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATT                                                                                       <  SRR19973069.1995696/150‑1 (MQ=60)
                                                          GTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTT                                                                                      >  SRR19973069.3396981/1‑150 (MQ=60)
                                                             TGGGTGGTGTTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTAACAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTAC                                                                                   <  SRR19973069.2873538/150‑1 (MQ=60)
                                                                 TGGTGGTTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAT                                                                               <  SRR19973069.2568457/150‑1 (MQ=60)
                                                                  GGTGGTTTGTGTTGCCATGAGGGCTTCTCACCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATT                                                                              <  SRR19973069.2880790/150‑1 (MQ=60)
                                                                   GTTGTTTGTGTGGCCATGAGGGCTTATCTCCAGTGAAAAATTGTGCGACTGTGTTTTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTG                                                                             <  SRR19973069.2350846/150‑1 (MQ=60)
                                                                   GTGTTTTGTGTTGCCAGGTGGGCTTCTCTCCAGTGAAAAATAGGGCGACTGCGTTTTTATGCTTTGCACTGTACCAGTACCCGAGTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTG                                                                             <  SRR19973069.2350881/150‑1 (MQ=60)
                                                                     GGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTG                                                                           >  SRR19973069.1499731/1‑150 (MQ=60)
                                                                     GGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTG                                                                           >  SRR19973069.114182/1‑150 (MQ=60)
                                                                     GGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATAGGGGATGTATATATTATTTTACAGATTGTG                                                                           >  SRR19973069.2056782/1‑150 (MQ=60)
                                                                       gttgtgttgcgttgcggggTTCTCTCGAGTTAAAAATTGTGCGATTGGGTTGTTTTGCATTGCACTGTACCAGTCCCCGCGTCAAAAAGAACCAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTT                                                                         <  SRR19973069.2175035/131‑1 (MQ=60)
                                                                       TTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCTTTTTTATGCTTTGCAGTGTACCAGTACCCGATTAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTT                                                                         <  SRR19973069.1993357/150‑1 (MQ=60)
                                                                        TAGTGTTGCCTTGCGGGCTTCTCTCCATTTAAAAATTGTGCGACTGCGTTGTTATGAATGGAAGTGTACCAGTACCCGATAACAAAACACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTC                                                                        <  SRR19973069.2678148/150‑1 (MQ=60)
                                                                         AGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCG                                                                       >  SRR19973069.1493881/1‑150 (MQ=60)
                                                                          GTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTTTTATGCATTGCACTGTACCAGTACCCGAGTAAAAAAGACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGC                                                                      <  SRR19973069.306357/150‑1 (MQ=60)
                                                                          GTGTTGCCATGAGGGATTCTCTCCAGTGAAAAATAGTGCGACTGCGTTTTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGC                                                                      <  SRR19973069.306337/150‑1 (MQ=60)
                                                                            GTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTG                                                                    <  SRR19973069.1564799/150‑1 (MQ=60)
                                                                              TGCCTTGAGGGTTTCTCTCCTTTTAAAAATTGTGCGACTGCGTTGTTATGCATTGCACTGTACCATTACCCTAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTT                                                                  <  SRR19973069.444009/150‑1 (MQ=60)
                                                                              TGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTT                                                                  >  SRR19973069.677257/1‑150 (MQ=60)
                                                                               GCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTC                                                                 >  SRR19973069.3434488/1‑150 (MQ=60)
                                                                                 CATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAG                                                               >  SRR19973069.2450020/1‑150 (MQ=60)
                                                                                      GGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGCGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATG                                                          <  SRR19973069.2230503/150‑1 (MQ=60)
                                                                                      GGGCTGCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTAGGCATTGCACTGTACCAGTACCCGACTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATG                                                          <  SRR19973069.1843460/150‑1 (MQ=60)
                                                                                       GGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGA                                                         >  SRR19973069.2474234/1‑150 (MQ=60)
                                                                                        GCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTCTATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAT                                                        >  SRR19973069.3403391/1‑150 (MQ=60)
                                                                                        GCTTCTCCCCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATTGCACGGTACCAGACCCCGAGAACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAT                                                        <  SRR19973069.3260618/150‑1 (MQ=60)
                                                                                         CTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATT                                                       >  SRR19973069.541156/1‑150 (MQ=60)
                                                                                         CTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATT                                                       >  SRR19973069.541162/1‑150 (MQ=60)
                                                                                         CTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATT                                                       >  SRR19973069.101402/1‑150 (MQ=60)
                                                                                         CTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATT                                                       >  SRR19973069.2359619/1‑150 (MQ=60)
                                                                                            CTCTCCAGTGAAAAATAGTGCGACTGCTTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACG                                                    <  SRR19973069.3078741/150‑1 (MQ=60)
                                                                                            CTCTCCAGTGAAAAATAGTGCGACTGCGTTTTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACG                                                    <  SRR19973069.530599/150‑1 (MQ=60)
                                                                                            CTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACG                                                    >  SRR19973069.26887/1‑150 (MQ=60)
                                                                                            CTCTCCAGTGAAAAATAGTGCGACGGCGTTTTTATGCTTTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACG                                                    <  SRR19973069.1403158/150‑1 (MQ=60)
                                                                                              CTCCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATGGCACTGTACCAGTACCCGATAAAAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCA                                                  <  SRR19973069.1445950/150‑1 (MQ=60)
                                                                                                        AAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGA                                        >  SRR19973069.1231675/1‑150 (MQ=60)
                                                                                                        AAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGA                                        >  SRR19973069.57359/1‑150 (MQ=60)
                                                                                                        AAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGA                                        >  SRR19973069.57343/1‑150 (MQ=60)
                                                                                                            AGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACG                                    >  SRR19973069.2657312/1‑150 (MQ=60)
                                                                                                             GTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGA                                   >  SRR19973069.667311/1‑150 (MQ=60)
                                                                                                              TGCGACTGCGTTTTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGAC                                  <  SRR19973069.2737147/150‑1 (MQ=60)
                                                                                                                 GACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGAC                               >  SRR19973069.928761/1‑150 (MQ=60)
                                                                                                                      CGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCG                          >  SRR19973069.3080883/1‑150 (MQ=60)
                                                                                                                       GTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGG                         >  SRR19973069.1554674/1‑150 (MQ=60)
                                                                                                                       GTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGG                         >  SRR19973069.1554708/1‑150 (MQ=60)
                                                                                                                         TGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTA                       >  SRR19973069.167045/1‑150 (MQ=60)
                                                                                                                         TGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTA                       >  SRR19973069.167043/1‑150 (MQ=60)
                                                                                                                         TGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTA                       >  SRR19973069.3454055/1‑150 (MQ=60)
                                                                                                                          GTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAG                      >  SRR19973069.1609989/1‑150 (MQ=60)
                                                                                                                          GTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAG                      >  SRR19973069.1405933/1‑150 (MQ=60)
                                                                                                                                 ATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATC               >  SRR19973069.3152082/1‑150 (MQ=60)
                                                                                                                                 ATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATC               >  SRR19973069.1357596/1‑150 (MQ=60)
                                                                                                                                 ATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATC               >  SRR19973069.314511/1‑150 (MQ=60)
                                                                                                                                   TGCACTGTACCAGTACCCGAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCAC             <  SRR19973069.2240003/150‑1 (MQ=60)
                                                                                                                                   TGCACTGTACCAGAACCCGATTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCAC             <  SRR19973069.638999/150‑1 (MQ=60)
                                                                                                                                   TGAACTGTACCAGAACCCGTGTAAAAACGACACAAAAAATGCCCAGATGGTAAAACTCGGGGATTTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCTTAACCACCGAAACGACGACGACCGGTAGAATCATCAC             <  SRR19973069.2239968/150‑1 (MQ=60)
                                                                                                                                    GCACTGTACCAGTACCCGAGTACAACAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACG            <  SRR19973069.268485/150‑1 (MQ=60)
                                                                                                                                     CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGA           >  SRR19973069.3669307/1‑150 (MQ=60)
                                                                                                                                     CACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGA           >  SRR19973069.3669399/1‑150 (MQ=60)
                                                                                                                                      ACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAC          >  SRR19973069.482550/1‑150 (MQ=60)
                                                                                                                                      ACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAC          >  SRR19973069.462859/1‑150 (MQ=60)
                                                                                                                                      ACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAC          >  SRR19973069.3793333/1‑150 (MQ=60)
                                                                                                                                        TGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGC        >  SRR19973069.3155560/1‑150 (MQ=60)
                                                                                                                                            CCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAG    >  SRR19973069.2032560/1‑150 (MQ=60)
                                                                                                                                            CCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAG    >  SRR19973069.2032517/1‑150 (MQ=60)
                                                                                                                                              AGTACCCGATAACAAAACACACAAAAAAAGCCCCGATGGTAAAAATGGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  <  SRR19973069.1214973/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                
CCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299093‑3299384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑