Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,266,318 G→T 100% R307L (CGT→CTT)  yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,266,3180GT98.9% 932.5 / 0.4 284R307L (CGT→CTT) yjcCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (147/134);  total (148/136)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 289 total aligned reads displayed.

TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGA  >  CP009273/4266173‑4266462
                                                                                                                                                 |                                                                                                                                                
ttACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACttt                                                                                                                                                >  2:1137492/1‑148 (MQ=255)
   ctctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGc                                                                                                                                             <  1:1800071/148‑1 (MQ=255)
    tctGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGcc                                                                                                                                            >  2:2202134/1‑148 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           >  2:2285402/1‑148 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           <  1:1986704/148‑1 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           <  2:1365916/148‑1 (MQ=255)
        gCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGt                                                                                                                                        <  2:79126/148‑1 (MQ=255)
             gTATTCGAAAAAACTATTTATCACCAAAACGTAAATTGCAACGCGCCCCCGAAAAACATCAACTTTGTCTTTTTTACCATCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCa                                                                                                                                   <  1:2700304/148‑1 (MQ=255)
              tATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAg                                                                                                                                  <  1:1022617/148‑1 (MQ=255)
              tATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAg                                                                                                                                  >  1:2643357/1‑148 (MQ=255)
               aTTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgg                                                                                                                                 <  1:559990/148‑1 (MQ=255)
                 tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCGTGGTGAGCAgggg                                                                                                                               >  2:758606/1‑148 (MQ=255)
                 tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgggg                                                                                                                               <  2:49280/148‑1 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            <  2:2750211/148‑1 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            >  2:1961306/1‑148 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            <  2:3758820/148‑1 (MQ=255)
                        aaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTGGTTACTTTGGCCTGGTGAGCAGGGGCAaataa                                                                                                                        >  2:395693/1‑148 (MQ=255)
                        aaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACAAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaataa                                                                                                                        <  1:434305/148‑1 (MQ=255)
                         aCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaataat                                                                                                                       >  1:1271786/1‑148 (MQ=255)
                           tATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGa                                                                                                                     <  1:1745916/148‑1 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    >  2:755534/1‑148 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    >  1:3326486/1‑148 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    <  2:2266565/148‑1 (MQ=255)
                              ttATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATc                                                                                                                  <  2:3088744/148‑1 (MQ=255)
                                 tctcCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCgg                                                                                                               >  1:1592419/1‑148 (MQ=255)
                                      cAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCagag                                                                                                          <  2:564077/148‑1 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                        >  2:2792693/1‑148 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                        <  2:2232863/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                       <  2:206863/148‑1 (MQ=255)
                                           gTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAt                                                                                                     <  1:2070295/148‑1 (MQ=255)
                                            tAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAtt                                                                                                    <  2:3078399/148‑1 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   <  2:3158621/148‑1 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   >  2:3645917/1‑148 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   >  1:753923/1‑148 (MQ=255)
                                               aTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                 >  2:1193634/1‑148 (MQ=255)
                                               aTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                 >  2:2379644/1‑148 (MQ=255)
                                                 tGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCt                                                                                               >  2:1665761/1‑148 (MQ=255)
                                                     aCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCa                                                                                           >  1:2190517/1‑148 (MQ=255)
                                                          cccTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaaaa                                                                                      <  1:614204/148‑1 (MQ=255)
                                                              cGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAaggag                                                                                  >  1:3127757/1‑148 (MQ=255)
                                                                  aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                              <  1:3713692/148‑1 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            >  2:1799961/1‑148 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            >  2:2698747/1‑148 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            <  1:1450806/148‑1 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  1:2792693/148‑1 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           >  2:2956315/1‑148 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  2:184/148‑1 (MQ=255)
                                                                     cATCAACTGTGTCATTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  2:2430814/148‑1 (MQ=255)
                                                                       tCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATa                                                                         >  2:1004185/1‑148 (MQ=255)
                                                                         aaCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGa                                                                       <  2:433800/148‑1 (MQ=255)
                                                                          aCTTTGTCTTTATTCCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCGGGCAGAAAAGGAGGGGATGATAGaa                                                                      <  1:1477697/148‑1 (MQ=255)
                                                                            tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                    >  2:3029052/1‑148 (MQ=255)
                                                                            tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTAATTTGGACTTGTGATCAGGGGCAAATAATTAATCCGGCAGAGTTTATTACGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                    >  1:2922529/1‑148 (MQ=255)
                                                                             ttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAg                                                                   <  1:3280658/148‑1 (MQ=255)
                                                                               gTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCTGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGAt                                                                 <  1:1825975/148‑1 (MQ=255)
                                                                                tCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATa                                                                >  2:466426/1‑148 (MQ=255)
                                                                                tCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATa                                                                <  2:3711306/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               <  2:1701635/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               >  2:1245413/1‑148 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               >  2:3765550/1‑148 (MQ=255)
                                                                                  tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                              <  2:619815/148‑1 (MQ=255)
                                                                                  tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                              <  1:3604366/148‑1 (MQ=255)
                                                                                   ttattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAAAAGATAACt                                                             >  1:655662/1‑148 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          <  1:404123/148‑1 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          <  1:3503705/148‑1 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          >  1:1522046/1‑148 (MQ=255)
                                                                                       taCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                         <  1:2853441/148‑1 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        <  2:1581368/148‑1 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:837797/1‑148 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:3172204/1‑148 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:1807173/1‑148 (MQ=255)
                                                                                         ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                       <  2:1425755/148‑1 (MQ=255)
                                                                                         ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                       <  2:1062271/148‑1 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                  <  2:2887686/148‑1 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                  >  1:3040312/1‑148 (MQ=255)
                                                                                                  aatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGAt                                              >  1:3427435/1‑148 (MQ=255)
                                                                                                    tCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATaa                                            >  1:2446507/1‑148 (MQ=255)
                                                                                                       atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                         <  1:441189/148‑1 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGACTCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                      >  2:3531428/1‑148 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                      >  2:3530179/1‑148 (MQ=255)
                                                                                                           cAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTc                                     >  1:1666536/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                  >  1:1062766/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGGTGTTAATTTGGCCAGGGGAGCACGGGCAAATAATGAATCCGGCACAGATTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                  >  1:2981108/1‑148 (MQ=255)
                                                                                                               aCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgcg                                 >  2:1869954/1‑148 (MQ=255)
                                                                                                                 aGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAt                               <  1:1134648/148‑1 (MQ=255)
                                                                                                                   aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCt                             >  2:2053816/1‑148 (MQ=255)
                                                                                                                   aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCt                             >  1:3213690/1‑148 (MQ=255)
                                                                                                                    aaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTg                            <  2:2554247/148‑1 (MQ=255)
                                                                                                                       aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                         >  2:2364079/1‑148 (MQ=255)
                                                                                                                         gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                       <  2:3336547/148‑1 (MQ=255)
                                                                                                                         gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                       >  1:2828648/1‑148 (MQ=255)
                                                                                                                          tATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAt                      <  1:770998/148‑1 (MQ=255)
                                                                                                                           aTCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAtt                     >  2:3616220/1‑148 (MQ=255)
                                                                                                                                          ttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATg      >  2:779260/1‑148 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTTATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     <  1:2528764/148‑1 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     >  2:911448/1‑148 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     <  2:3437289/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:1915592/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:3697925/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:582355/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGA  >  CP009273/4266173‑4266462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCG  >  CP009273/4266169‑4266465
                                                                                                                                                     |                                                                                                                                                   
GCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACT                                                                                                                                                     <  SRR19973069.992035/150‑1 (MQ=60)
GCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACT                                                                                                                                                     <  SRR19973069.992024/150‑1 (MQ=60)
 CTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTT                                                                                                                                                    >  SRR19973069.1098315/1‑150 (MQ=60)
  TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTT                                                                                                                                                   <  SRR19973069.2665968/150‑1 (MQ=60)
  TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTT                                                                                                                                                   >  SRR19973069.309872/1‑150 (MQ=60)
   ATTACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTG                                                                                                                                                  >  SRR19973069.3150973/1‑150 (MQ=60)
    TTACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGG                                                                                                                                                 >  SRR19973069.6572/1‑150 (MQ=60)
      ACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCTATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGTC                                                                                                                                               >  SRR19973069.3333713/1‑150 (MQ=60)
      ACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCTATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGTC                                                                                                                                               >  SRR19973069.331755/1‑150 (MQ=60)
       CTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCT                                                                                                                                              <  SRR19973069.1812746/150‑1 (MQ=60)
         CTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGG                                                                                                                                            <  SRR19973069.2000540/150‑1 (MQ=60)
                CGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAG                                                                                                                                     >  SRR19973069.2661061/1‑150 (MQ=60)
                 GTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGG                                                                                                                                    <  SRR19973069.410771/150‑1 (MQ=60)
                 GTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGG                                                                                                                                    <  SRR19973069.2718410/150‑1 (MQ=60)
                 GTATTCGAAAAAACTATTTATCACCAAAACGTAAATTGCAACGCGCCCCCGAAAAACATCAACTTTGTCTTTTTTACCATCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCATG                                                                                                                                    <  SRR19973069.2718399/150‑1 (MQ=60)
                  TATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGG                                                                                                                                   <  SRR19973069.1029690/150‑1 (MQ=60)
                  TATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGG                                                                                                                                   >  SRR19973069.3735550/1‑150 (MQ=60)
                   ATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGG                                                                                                                                  <  SRR19973069.563891/150‑1 (MQ=60)
                       GACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAA                                                                                                                              >  SRR19973069.2871338/1‑150 (MQ=60)
                        ACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCACAT                                                                                                                             >  SRR19973069.1625417/1‑150 (MQ=60)
                        ACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAAT                                                                                                                             <  SRR19973069.2363109/150‑1 (MQ=60)
                           AAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAAT                                                                                                                          >  SRR19973069.1280330/1‑150 (MQ=60)
                            AACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACAAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATG                                                                                                                         <  SRR19973069.437491/150‑1 (MQ=60)
                              CTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAA                                                                                                                       >  SRR19973069.3348286/1‑150 (MQ=60)
                               TATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAT                                                                                                                      <  SRR19973069.2635300/150‑1 (MQ=60)
                               TATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAT                                                                                                                      >  SRR19973069.1060621/1‑150 (MQ=60)
                               TATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAT                                                                                                                      <  SRR19973069.1758188/150‑1 (MQ=60)
                               TATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAT                                                                                                                      <  SRR19973069.1758080/150‑1 (MQ=60)
                                 TTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCC                                                                                                                    <  SRR19973069.284069/150‑1 (MQ=60)
                                 TTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCC                                                                                                                    <  SRR19973069.2226738/150‑1 (MQ=60)
                                   TATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGG                                                                                                                  >  SRR19973069.1603184/1‑150 (MQ=60)
                                       TCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGA                                                                                                              >  SRR19973069.3033718/1‑150 (MQ=60)
                                         CCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGT                                                                                                            >  SRR19973069.772296/1‑150 (MQ=60)
                                         CCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGT                                                                                                            >  SRR19973069.391086/1‑150 (MQ=60)
                                           AAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTT                                                                                                          >  SRR19973069.1879138/1‑150 (MQ=60)
                                             ACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAT                                                                                                        <  SRR19973069.2926517/150‑1 (MQ=60)
                                               GTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTC                                                                                                      >  SRR19973069.759254/1‑150 (MQ=60)
                                               GTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTC                                                                                                      <  SRR19973069.2084643/150‑1 (MQ=60)
                                               GTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTC                                                                                                      <  SRR19973069.3529578/150‑1 (MQ=60)
                                                       CAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCA                                                                                              >  SRR19973069.726025/1‑150 (MQ=60)
                                                       CAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCA                                                                                              >  SRR19973069.2205424/1‑150 (MQ=60)
                                                             GCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAG                                                                                        <  SRR19973069.148120/150‑1 (MQ=60)
                                                              CCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGG                                                                                       <  SRR19973069.618308/150‑1 (MQ=60)
                                                              CCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGACAGAAAAGG                                                                                       >  SRR19973069.3670237/1‑150 (MQ=60)
                                                              CCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGACAGAAAAGG                                                                                       >  SRR19973069.464128/1‑150 (MQ=60)
                                                               CCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGA                                                                                      >  SRR19973069.507261/1‑150 (MQ=60)
                                                                CTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAG                                                                                     >  SRR19973069.3148279/1‑150 (MQ=60)
                                                                CTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAG                                                                                     <  SRR19973069.41873/150‑1 (MQ=60)
                                                                 TCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGG                                                                                    <  SRR19973069.90374/150‑1 (MQ=60)
                                                                     AAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGAT                                                                                >  SRR19973069.3407809/1‑150 (MQ=60)
                                                                     AAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGAT                                                                                <  SRR19973069.1092609/150‑1 (MQ=60)
                                                                      AAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCACAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATG                                                                               <  SRR19973069.2988619/150‑1 (MQ=60)
                                                                      AAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATG                                                                               <  SRR19973069.1880980/150‑1 (MQ=60)
                                                                      AAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATG                                                                               <  SRR19973069.3737944/150‑1 (MQ=60)
                                                                        ACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGAT                                                                             <  SRR19973069.1460935/150‑1 (MQ=60)
                                                                         CATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATA                                                                            <  SRR19973069.2811264/150‑1 (MQ=60)
                                                                          ATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAG                                                                           >  SRR19973069.3730992/1‑150 (MQ=60)
                                                                           TCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGA                                                                          >  SRR19973069.703410/1‑150 (MQ=60)
                                                                             AACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAAC                                                                        >  SRR19973069.77885/1‑150 (MQ=60)
                                                                              ACTTTGTCTTTATTCCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCGGGCAGAAAAGGAGGGGATGATAGAACT                                                                       <  SRR19973069.1487984/150‑1 (MQ=60)
                                                                              ACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACA                                                                       <  SRR19973069.1487872/150‑1 (MQ=60)
                                                                              ACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACA                                                                       >  SRR19973069.2941510/1‑150 (MQ=60)
                                                                              ACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACA                                                                       <  SRR19973069.1488388/150‑1 (MQ=60)
                                                                              ACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTAATTTGGACTTGTGATCAGGGGCAAATAATTAATCCGGCAGAGTTTATTACGCTGGCAGAAAAGGAGGGGATGATAGAACA                                                                       >  SRR19973069.2941885/1‑150 (MQ=60)
                                                                                 TTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGAT                                                                    <  SRR19973069.3302143/150‑1 (MQ=60)
                                                                                  TGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATA                                                                   <  SRR19973069.2818072/150‑1 (MQ=60)
                                                                                   GTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCTGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAA                                                                  <  SRR19973069.1838781/150‑1 (MQ=60)
                                                                                     CTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAAAAGATAACT                                                                >  SRR19973069.660065/1‑150 (MQ=60)
                                                                                      TTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTG                                                               <  SRR19973069.3627899/150‑1 (MQ=60)
                                                                                      TTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTG                                                               <  SRR19973069.3627207/150‑1 (MQ=60)
                                                                                        TATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAT                                                             >  SRR19973069.1532536/1‑150 (MQ=60)
                                                                                          TTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTA                                                           <  SRR19973069.3525852/150‑1 (MQ=60)
                                                                                          TTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTA                                                           <  SRR19973069.3526261/150‑1 (MQ=60)
                                                                                          TTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTA                                                           <  SRR19973069.407085/150‑1 (MQ=60)
                                                                                           TACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAT                                                          <  SRR19973069.2872222/150‑1 (MQ=60)
                                                                                           TACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAT                                                          <  SRR19973069.3521490/150‑1 (MQ=60)
                                                                                            ACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATG                                                         <  SRR19973069.2020699/150‑1 (MQ=60)
                                                                                            ACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATG                                                         <  SRR19973069.1191066/150‑1 (MQ=60)
                                                                                                GCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAT                                                     >  SRR19973069.3060457/1‑150 (MQ=60)
                                                                                                GCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAT                                                     >  SRR19973069.3060310/1‑150 (MQ=60)
                                                                                                  CAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTG                                                   >  SRR19973069.3721813/1‑150 (MQ=60)
                                                                                                    ATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGAT                                                 >  SRR19973069.3449724/1‑150 (MQ=60)
                                                                                                      AATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAA                                               >  SRR19973069.2463666/1‑150 (MQ=60)
                                                                                                      AATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAA                                               >  SRR19973069.2767879/1‑150 (MQ=60)
                                                                                                      AATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAA                                               >  SRR19973069.3775854/1‑150 (MQ=60)
                                                                                                      AATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAA                                               >  SRR19973069.2462978/1‑150 (MQ=60)
                                                                                                       ATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAAT                                              >  SRR19973069.1698602/1‑150 (MQ=60)
                                                                                                          GATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTC                                           >  SRR19973069.1783923/1‑150 (MQ=60)
                                                                                                          GATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTC                                           >  SRR19973069.297090/1‑150 (MQ=60)
                                                                                                           ATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCT                                          <  SRR19973069.444417/150‑1 (MQ=60)
                                                                                                           ATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCT                                          <  SRR19973069.443789/150‑1 (MQ=60)
                                                                                                            TATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTT                                         <  SRR19973069.2037531/150‑1 (MQ=60)
                                                                                                            TATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTT                                         <  SRR19973069.110806/150‑1 (MQ=60)
                                                                                                             ATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTC                                        >  SRR19973069.1678091/1‑150 (MQ=60)
                                                                                                             ATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTC                                        <  SRR19973069.2296559/150‑1 (MQ=60)
                                                                                                              TCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCC                                       <  SRR19973069.3354451/150‑1 (MQ=60)
                                                                                                               CAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCG                                      <  SRR19973069.2120974/150‑1 (MQ=60)
                                                                                                                AAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGC                                     >  SRR19973069.1070171/1‑150 (MQ=60)
                                                                                                                AAAACAGAAAAATGTATCGGCGCTGAAGCGGTGTTAATTTGGCCAGGGGAGCACGGGCAAATAATGAATCCGGCACAGATTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGC                                     >  SRR19973069.3000903/1‑150 (MQ=60)
                                                                                                                 AAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCG                                    <  SRR19973069.1677307/150‑1 (MQ=60)
                                                                                                                   ACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAT                                  >  SRR19973069.1601194/1‑150 (MQ=60)
                                                                                                                   ACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAT                                  >  SRR19973069.1601204/1‑150 (MQ=60)
                                                                                                                     AGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCT                                >  SRR19973069.3234655/1‑150 (MQ=60)
                                                                                                                     AGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCT                                <  SRR19973069.320692/150‑1 (MQ=60)
                                                                                                                     AGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCT                                <  SRR19973069.1142569/150‑1 (MQ=60)
                                                                                                                          AATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCG                           >  SRR19973069.3115211/1‑150 (MQ=60)
                                                                                                                           ATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGA                          >  SRR19973069.329130/1‑150 (MQ=60)
                                                                                                                           ATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGA                          >  SRR19973069.2847345/1‑150 (MQ=60)
                                                                                                                           ATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGA                          >  SRR19973069.578299/1‑150 (MQ=60)
                                                                                                                           ATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGA                          >  SRR19973069.2847348/1‑150 (MQ=60)
                                                                                                                              TATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTA                       <  SRR19973069.1430373/150‑1 (MQ=60)
                                                                                                                              TATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTA                       <  SRR19973069.776460/150‑1 (MQ=60)
                                                                                                                              TATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTA                       <  SRR19973069.590566/150‑1 (MQ=60)
                                                                                                                              TATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTA                       <  SRR19973069.2707865/150‑1 (MQ=60)
                                                                                                                               ATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTAC                      <  SRR19973069.618592/150‑1 (MQ=60)
                                                                                                                                   GCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGG                  <  SRR19973069.968498/150‑1 (MQ=60)
                                                                                                                                   GCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGG                  <  SRR19973069.1406052/150‑1 (MQ=60)
                                                                                                                                   GCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGG                  <  SRR19973069.1974901/150‑1 (MQ=60)
                                                                                                                                      CTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAA               >  SRR19973069.1940807/1‑150 (MQ=60)
                                                                                                                                         AAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACAC            >  SRR19973069.2618333/1‑150 (MQ=60)
                                                                                                                                           GCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAT          <  SRR19973069.2519050/150‑1 (MQ=60)
                                                                                                                                           GCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAT          <  SRR19973069.2172154/150‑1 (MQ=60)
                                                                                                                                            CGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATG         >  SRR19973069.1211918/1‑150 (MQ=60)
                                                                                                                                               TGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTTATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAG      <  SRR19973069.2545542/150‑1 (MQ=60)
                                                                                                                                               TGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAG      >  SRR19973069.1531640/1‑150 (MQ=60)
                                                                                                                                                GTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGA     >  SRR19973069.610582/1‑150 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.1819880/150‑1 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.586315/150‑1 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.1819887/150‑1 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.1928793/150‑1 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.586345/150‑1 (MQ=60)
                                                                                                                                                  TACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATC   <  SRR19973069.3722093/150‑1 (MQ=60)
                                                                                                                                                   ACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCG  <  SRR19973069.3025906/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCG  >  CP009273/4266169‑4266465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑