Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,915,555 A→C 16.6% Q359P (CAG→CCG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,915,5550AC16.6% 790.5 / 166.9 325Q359P (CAG→CCG) rsmF16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (119/151);  new base C (28/26);  total (147/178)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

TAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCC  >  CP009273/1915406‑1915703
                                                                                                                                                     |                                                                                                                                                    
 aaaaCTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAg                                                                                                                                                     >  1:3173700/1‑150 (MQ=255)
  aaaCTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGc                                                                                                                                                    >  1:1890245/1‑150 (MQ=255)
     cTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTg                                                                                                                                                 <  2:4124819/150‑1 (MQ=255)
     cTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTg                                                                                                                                                 <  2:1498465/150‑1 (MQ=255)
      tCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGa                                                                                                                                                >  2:4995489/1‑150 (MQ=255)
       cAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGAc                                                                                                                                               >  1:2572882/1‑150 (MQ=255)
        aGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACa                                                                                                                                              >  1:1264617/1‑150 (MQ=255)
           cGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAg                                                                                                                                           >  1:2662545/1‑150 (MQ=255)
            gATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGa                                                                                                                                          >  1:3753293/1‑150 (MQ=255)
              ttCCCGCCTTACCCGCCCCCAAATACACAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAAc                                                                                                                                        <  2:4956181/150‑1 (MQ=255)
              ttCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAAc                                                                                                                                        <  1:4679496/150‑1 (MQ=255)
              ttCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAAc                                                                                                                                        <  1:2671999/150‑1 (MQ=255)
               tCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACt                                                                                                                                       <  1:4012566/150‑1 (MQ=255)
                  cGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACtgtg                                                                                                                                    >  2:4618250/1‑150 (MQ=255)
                  cGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACtgtg                                                                                                                                    >  2:647648/1‑150 (MQ=255)
                    ccTGACCCGCCCCCCAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgt                                                                                                                                  <  2:4360512/150‑1 (MQ=255)
                     cTTACCCGCCCCCAAATCCAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgtt                                                                                                                                 <  1:2536505/150‑1 (MQ=255)
                       tACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgttgt                                                                                                                               >  2:4035183/1‑150 (MQ=255)
                       tACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgttgt                                                                                                                               >  2:5186702/1‑150 (MQ=255)
                        aCCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgttgtt                                                                                                                              <  2:5648410/150‑1 (MQ=255)
                        aCCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGgttgtt                                                                                                                              <  1:4203085/150‑1 (MQ=255)
                         cccGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTc                                                                                                                             <  2:4093193/150‑1 (MQ=255)
                           cGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTccc                                                                                                                           <  1:5422560/150‑1 (MQ=255)
                            gCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCg                                                                                                                          <  2:166956/150‑1 (MQ=255)
                               cccAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTg                                                                                                                       >  1:2390758/1‑150 (MQ=255)
                               cccAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTg                                                                                                                       >  1:3687091/1‑150 (MQ=255)
                               cccAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTg                                                                                                                       <  2:1365838/150‑1 (MQ=255)
                                ccAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTgg                                                                                                                      <  1:3291299/150‑1 (MQ=255)
                                 cAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTggg                                                                                                                     <  1:1357161/150‑1 (MQ=255)
                                  aaaTACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGc                                                                                                                    <  2:5213292/150‑1 (MQ=255)
                                  aaaTACAAAGTCGGTAATTTTCCGTTCAGACCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACCAAGAACTGTGGTTGTTCCCGGTGGGc                                                                                                                    >  2:1978511/1‑150 (MQ=255)
                                      aCAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTg                                                                                                                <  2:5373820/150‑1 (MQ=255)
                                      aCAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTg                                                                                                                >  2:5591478/1‑150 (MQ=255)
                                       cAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGa                                                                                                               >  2:2654563/1‑150 (MQ=255)
                                        aaaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGaa                                                                                                              >  1:1777212/1‑150 (MQ=255)
                                         aaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAg                                                                                                             >  2:1788032/1‑150 (MQ=255)
                                         aaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAg                                                                                                             >  1:4147260/1‑150 (MQ=255)
                                         aaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAg                                                                                                             >  1:1702600/1‑150 (MQ=255)
                                          aGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGc                                                                                                            <  2:1267865/150‑1 (MQ=255)
                                             cGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCt                                                                                                         >  2:3815427/1‑150 (MQ=255)
                                                tAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGAt                                                                                                      <  2:3167406/150‑1 (MQ=255)
                                                tAATTTTCAGTTCAGCCCTGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCTCCTATGGCCGCGTGACATAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTgtt                                                                                                      <  2:2764443/150‑3 (MQ=255)
                                                     ttCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTa                                                                                                 <  2:2004648/150‑1 (MQ=255)
                                                      tCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTaa                                                                                                >  1:4650229/1‑150 (MQ=255)
                                                       ccGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTaaa                                                                                               >  1:5331658/1‑150 (MQ=255)
                                                       ccGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTaaa                                                                                               <  1:457223/150‑1 (MQ=255)
                                                         gTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGt                                                                                             <  1:4248568/150‑1 (MQ=255)
                                                         gTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAAATCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGt                                                                                             >  1:702505/1‑150 (MQ=255)
                                                           tCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTcc                                                                                           >  1:5154739/1‑150 (MQ=255)
                                                            cAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACATGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCg                                                                                          <  1:478138/150‑1 (MQ=255)
                                                             aGCCCGGTGAAAGATCGTGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGa                                                                                         >  2:3279063/1‑150 (MQ=255)
                                                               cccGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGAtt                                                                                       <  2:2537170/150‑1 (MQ=255)
                                                               cccGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGAtt                                                                                       <  2:5679592/150‑1 (MQ=255)
                                                                 cGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGAtttt                                                                                     >  1:2199506/1‑150 (MQ=255)
                                                                   gTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTtct                                                                                   >  1:5576277/1‑150 (MQ=255)
                                                                     gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCg                                                                                 >  1:78139/1‑150 (MQ=255)
                                                                     gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCg                                                                                 >  2:1544052/1‑150 (MQ=255)
                                                                     gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCg                                                                                 <  1:3399535/150‑1 (MQ=255)
                                                                      aaaGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCgg                                                                                <  2:1890245/150‑1 (MQ=255)
                                                                       aaGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGt                                                                               <  2:1174899/150‑1 (MQ=255)
                                                                        aGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCCTTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGtt                                                                              >  2:5536444/1‑150 (MQ=255)
                                                                          aTCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTgg                                                                            >  1:369892/1‑150 (MQ=255)
                                                                          aTCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTgg                                                                            <  2:4546666/150‑1 (MQ=255)
                                                                           tCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTAGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCAGATTTTCTCTGTTggg                                                                           >  1:1025417/1‑150 (MQ=255)
                                                                             gcgAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGa                                                                         <  2:5301935/150‑1 (MQ=255)
                                                                                   cTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAAc                                                                   <  1:1599383/150‑1 (MQ=255)
                                                                                    tGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACt                                                                  <  2:352687/150‑1 (MQ=255)
                                                                                      gACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTg                                                                <  1:300377/150‑1 (MQ=255)
                                                                                          aaTTCGTCAGGCGGCTACAGGTGTTGGCTTAACCGGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCga                                                            <  2:5433973/150‑1 (MQ=255)
                                                                                           aTTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCgag                                                           >  1:660878/1‑150 (MQ=255)
                                                                                              cGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACg                                                        >  2:1905729/1‑150 (MQ=255)
                                                                                              cGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACg                                                        >  1:615046/1‑150 (MQ=255)
                                                                                                  aGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGcaca                                                    <  2:4756730/150‑1 (MQ=255)
                                                                                                       gcTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACaaa                                               >  2:5468048/1‑150 (MQ=255)
                                                                                                       gcTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACaaa                                               <  1:1081439/150‑1 (MQ=255)
                                                                                                       gcTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACaaa                                               <  1:3586229/150‑1 (MQ=255)
                                                                                                         tACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAgg                                             <  2:4436245/150‑1 (MQ=255)
                                                                                                           cAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGtt                                           <  2:8591/150‑1 (MQ=255)
                                                                                                           cAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGtt                                           <  1:899595/150‑1 (MQ=255)
                                                                                                               tgtTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCg                                       <  1:2369739/150‑1 (MQ=255)
                                                                                                                 ttGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCt                                     <  1:4058932/150‑1 (MQ=255)
                                                                                                                   ggCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTgg                                   >  2:375215/1‑150 (MQ=255)
                                                                                                                    gCTTAAACTGGGATGAAAACCAGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGgc                                  <  2:3914237/150‑1 (MQ=255)
                                                                                                                         aaCTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCAt                             <  1:5497919/150‑1 (MQ=255)
                                                                                                                           cTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGa                           <  1:2943285/150‑1 (MQ=255)
                                                                                                                              ggATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGc                        >  2:3637173/1‑150 (MQ=255)
                                                                                                                               gATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCg                       >  2:2221344/1‑150 (MQ=255)
                                                                                                                               gATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCg                       <  1:647600/150‑1 (MQ=255)
                                                                                                                                  gAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGtt                    <  2:1333531/150‑1 (MQ=255)
                                                                                                                                  gAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGtt                    <  1:2046499/150‑1 (MQ=255)
                                                                                                                                   aaaaCCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTa                   <  2:4239468/150‑1 (MQ=255)
                                                                                                                                       ccTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGc               >  1:605729/1‑150 (MQ=255)
                                                                                                                                       ccTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGc               >  1:114294/1‑150 (MQ=255)
                                                                                                                                       ccTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGGTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGc               >  2:2987512/1‑150 (MQ=255)
                                                                                                                                          gcgcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTCAACTTGACGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGctcg            >  2:4848219/1‑149 (MQ=255)
                                                                                                                                            gcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTg          >  1:4344502/1‑150 (MQ=255)
                                                                                                                                            gcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCGTg          >  2:2416800/1‑150 (MQ=255)
                                                                                                                                              ctctGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGcc        <  1:2300241/150‑1 (MQ=255)
                                                                                                                                               tctGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCt       >  2:77075/1‑150 (MQ=255)
                                                                                                                                                 tGGCCGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCa     <  1:4995489/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCC  >  CP009273/1915406‑1915703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A