Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,654,144 G→T 7.2% I249I (ATC→ATA)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,654,1440GT 7.2% 1166.2 / 43.5 308I249I (ATC→ATA) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (139/146);  new base T (13/10);  total (152/156)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

TTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACAT  >  CP009273/2653995‑2654293
                                                                                                                                                     |                                                                                                                                                     
ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCcgacg                                                                                                                                                       >  1:1857726/1‑150 (MQ=255)
  cGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGAt                                                                                                                                                     >  2:558248/1‑150 (MQ=255)
   ggCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATc                                                                                                                                                    <  1:331580/150‑1 (MQ=255)
   ggCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATc                                                                                                                                                    >  1:1783767/1‑150 (MQ=255)
     cgcACCGTGTTCCAGGTCACCGGTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTg                                                                                                                                                  >  2:24078/1‑150 (MQ=255)
      gcACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCTATGCGATAGGCCTCGCCCATTCCGACGATCTgg                                                                                                                                                 >  2:5298247/1‑150 (MQ=255)
      gcACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTgg                                                                                                                                                 <  2:3775353/150‑1 (MQ=255)
        aCCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTg                                                                                                                                               >  1:3453951/1‑150 (MQ=255)
        aCCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTg                                                                                                                                               <  2:1841118/150‑1 (MQ=255)
          cGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGaa                                                                                                                                             >  2:2225209/1‑150 (MQ=255)
          cGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTgga                                                                                                                                             >  2:2797928/1‑148 (MQ=255)
          cGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGaa                                                                                                                                             >  1:5375650/1‑150 (MQ=255)
          cGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGaa                                                                                                                                             >  1:4526114/1‑150 (MQ=255)
              ttCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAgg                                                                                                                                         <  1:4797076/150‑1 (MQ=255)
               tCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGc                                                                                                                                        >  2:2340942/1‑150 (MQ=255)
               tCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGc                                                                                                                                        >  2:5181613/1‑150 (MQ=255)
                 cAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCag                                                                                                                                      <  1:2778621/150‑1 (MQ=255)
                 cAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCag                                                                                                                                      >  2:1534066/1‑150 (MQ=255)
                   ggTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCggag                                                                                                                                    <  2:1556555/150‑1 (MQ=255)
                   ggTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCagag                                                                                                                                    <  2:3801354/150‑1 (MQ=255)
                    gTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGt                                                                                                                                   <  2:4915229/150‑1 (MQ=255)
                        ccGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCg                                                                                                                               >  2:5003045/1‑150 (MQ=255)
                          gTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGa                                                                                                                             <  2:2649155/150‑1 (MQ=255)
                           ttCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGaa                                                                                                                            >  2:4152767/1‑150 (MQ=255)
                           ttCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGaa                                                                                                                            <  1:3904647/150‑1 (MQ=255)
                            tCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAAc                                                                                                                           <  1:1840710/150‑1 (MQ=255)
                                gTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCAt                                                                                                                       >  2:5496229/1‑150 (MQ=255)
                                gTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCAt                                                                                                                       <  2:4745414/150‑1 (MQ=255)
                                 tAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATa                                                                                                                      >  1:252962/1‑150 (MQ=255)
                                    aCTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACcg                                                                                                                   >  1:1261018/1‑150 (MQ=255)
                                      ttcttcGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACcgcg                                                                                                                 <  1:1352468/150‑1 (MQ=255)
                                        cttcGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCt                                                                                                               >  1:2306311/1‑150 (MQ=255)
                                        cttcGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCt                                                                                                               >  2:1719493/1‑150 (MQ=255)
                                         ttcGACATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTc                                                                                                              <  1:1765826/150‑1 (MQ=255)
                                            gATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTg                                                                                                           <  2:3177394/150‑1 (MQ=255)
                                               atCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGACCGCATACCGCGCTCGTGAcc                                                                                                        >  2:3991802/1‑150 (MQ=255)
                                                tCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccg                                                                                                       <  1:3576888/150‑1 (MQ=255)
                                                 cTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgc                                                                                                      >  2:1290791/1‑150 (MQ=255)
                                                   ttGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccg                                                                                                    <  1:5585890/150‑1 (MQ=255)
                                                     gATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgcc                                                                                                  >  1:2918578/1‑150 (MQ=255)
                                                     gATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgcc                                                                                                  >  1:2938273/1‑150 (MQ=255)
                                                      aTGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGAccgccgccg                                                                                                 >  1:4335069/1‑150 (MQ=255)
                                                        gCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTg                                                                                               >  2:3239872/1‑150 (MQ=255)
                                                          cGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCa                                                                                             >  1:3293623/1‑150 (MQ=255)
                                                          cGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCa                                                                                             <  2:1186959/150‑1 (MQ=255)
                                                          cGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCa                                                                                             >  2:1973193/1‑150 (MQ=255)
                                                           gTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAt                                                                                            >  1:1384551/1‑150 (MQ=255)
                                                            ttCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATATGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAtt                                                                                           >  1:2058068/1‑150 (MQ=255)
                                                              ccACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTg                                                                                         >  2:682736/1‑150 (MQ=255)
                                                                 caGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTgcgc                                                                                      >  1:5001534/1‑150 (MQ=255)
                                                                  aGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCt                                                                                     <  1:2615871/150‑1 (MQ=255)
                                                                   gACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCtt                                                                                    >  1:2394418/1‑150 (MQ=255)
                                                                    aCGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTc                                                                                   <  1:2789524/150‑1 (MQ=255)
                                                                    aCGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGACCAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCAGTTGCGCTTc                                                                                   >  2:1395334/1‑150 (MQ=255)
                                                                    aCGGTTACGCAGGCCGCGCAGACGGTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTc                                                                                   <  1:3803261/150‑1 (MQ=255)
                                                                      ggTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGa                                                                                 >  1:2037696/1‑150 (MQ=255)
                                                                       gTTACGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGAt                                                                                <  2:2108217/150‑1 (MQ=255)
                                                                           cGCAGGCCGCGCAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGt                                                                            <  1:2333021/150‑1 (MQ=255)
                                                                             cAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTAc                                                                          <  1:5246177/150‑1 (MQ=255)
                                                                                     gcAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGtt                                                                  <  1:4495036/150‑1 (MQ=255)
                                                                                     gcAGACGTTCCATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGtt                                                                  <  2:3503744/150‑1 (MQ=255)
                                                                                      cAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGttt                                                                 <  2:481569/150‑1 (MQ=255)
                                                                                        gACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTac                                                               <  2:3949738/150‑1 (MQ=255)
                                                                                          cGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTacga                                                             >  2:2754010/1‑150 (MQ=255)
                                                                                           gTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTacgac                                                            >  1:1162969/1‑150 (MQ=255)
                                                                                               cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACTATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtac                                                        <  1:3047575/150‑1 (MQ=255)
                                                                                               cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtac                                                        <  1:847561/150‑1 (MQ=255)
                                                                                               cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtac                                                        <  2:252962/150‑1 (MQ=255)
                                                                                               cATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtac                                                        <  1:732553/150‑1 (MQ=255)
                                                                                               cATCTCGGTAGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtac                                                        <  2:43565/150‑1 (MQ=255)
                                                                                                   tcGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacatac                                                    <  1:1005402/150‑1 (MQ=255)
                                                                                                    cGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGtacataca                                                   >  1:3736119/1‑150 (MQ=255)
                                                                                                       tCGCCATCTCTTCTTTTGCGTTGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAgcg                                                <  1:3373597/150‑1 (MQ=255)
                                                                                                            aTCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCg                                           >  1:1925486/1‑150 (MQ=255)
                                                                                                             tctcTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGa                                          <  1:2592665/150‑1 (MQ=255)
                                                                                                              ctctTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGAt                                         >  2:742161/1‑150 (MQ=255)
                                                                                                              ctctTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGAt                                         >  1:4262876/1‑150 (MQ=255)
                                                                                                                cttcttTTGCGATGCGATAGGCCTCGCCCATTCCGTCGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATAc                                       <  2:5282360/150‑1 (MQ=255)
                                                                                                                   cttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCtt                                    >  2:3105192/1‑150 (MQ=255)
                                                                                                                    ttttGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCttt                                   >  2:3645648/1‑150 (MQ=255)
                                                                                                                         cgatgcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGc                              <  2:5455745/150‑1 (MQ=255)
                                                                                                                            tgcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCAt                           >  1:457856/1‑150 (MQ=255)
                                                                                                                             gcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATa                          <  2:1007491/150‑1 (MQ=255)
                                                                                                                              cgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCCTAg                         >  1:5390995/1‑150 (MQ=255)
                                                                                                                              cgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAg                         <  1:24078/150‑1 (MQ=255)
                                                                                                                                 tAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGAtt                      <  1:2604005/150‑1 (MQ=255)
                                                                                                                                    gCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTtg                   <  1:3112228/150‑1 (MQ=255)
                                                                                                                                    gCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTtg                   <  1:1534066/150‑1 (MQ=255)
                                                                                                                                    gCCGCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTtg                   >  2:601486/1‑150 (MQ=255)
                                                                                                                                       tCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGGTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGa                >  1:5119954/1‑150 (MQ=255)
                                                                                                                                        cGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGAc               <  1:898320/150‑1 (MQ=255)
                                                                                                                                         gCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGAcc              >  2:3474028/1‑150 (MQ=255)
                                                                                                                                          cccATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCg             <  1:682736/150‑1 (MQ=255)
                                                                                                                                            cATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACCTACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGga           >  2:5397578/1‑150 (MQ=255)
                                                                                                                                             aTTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGgag          >  1:1833544/1‑150 (MQ=255)
                                                                                                                                             aTTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGgag          <  1:4112329/150‑1 (MQ=255)
                                                                                                                                               tCCGACGATCTGGTGAACAGGCAGAGTGTCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGaa        >  2:4778366/1‑150 (MQ=255)
                                                                                                                                                ccGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGaaa       <  1:1570727/150‑1 (MQ=255)
                                                                                                                                                ccGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGaaa       >  1:3962280/1‑150 (MQ=255)
                                                                                                                                                    cgaTCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACa   <  2:866875/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACAT  >  CP009273/2653995‑2654293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A