Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 397,959 G→A 100% A253T (GCG→ACG)  phoA → bacterial alkaline phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273397,9590GA97.7% 846.9 / 6.5 263A253T (GCG→ACG) phoAbacterial alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base G (6/0);  new base A (151/104);  total (158/105)
Fisher's exact test for biased strand distribution p-value = 8.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

GCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATAT  >  CP009273/397812‑398104
                                                                                                                                                   |                                                                                                                                                 
 ctctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTAAACGCTCGTGCCGACGTTACTCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGacgc                                                                                                                                                >  1:1181896/1‑150 (MQ=255)
  tctGGATAAAGGCGGAAAAGGATCGATTACCGATCTGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAATCGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGAcgcg                                                                                                                                               <  2:1901329/150‑1 (MQ=255)
   ctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGt                                                                                                                                              <  2:4676631/150‑1 (MQ=255)
   ctGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGt                                                                                                                                              >  2:1772325/1‑150 (MQ=255)
     ggAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCGGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTgg                                                                                                                                            >  2:2349147/1‑150 (MQ=255)
       aaaaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGtt                                                                                                                                          >  2:133144/1‑150 (MQ=255)
         aaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTAt                                                                                                                                        >  2:4255421/1‑150 (MQ=255)
         aaaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTAt                                                                                                                                        >  2:4195138/1‑150 (MQ=255)
          aaGGCGGAAAAGGATCGATTACCGACCAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATc                                                                                                                                       <  1:132363/150‑1 (MQ=255)
          aaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGACACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATc                                                                                                                                       >  2:4867060/1‑150 (MQ=255)
          aaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATc                                                                                                                                       >  1:4937390/1‑150 (MQ=255)
          aaGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTATGCTGAAACGGCAAACGCTGGTGAAATGCAGGGAAAAACGCTGCGTGAACATGCACATACTCGTGGTAATc                                                                                                                                       >  1:4870895/1‑150 (MQ=255)
           aGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCa                                                                                                                                      >  1:1068654/1‑150 (MQ=255)
              cGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCTTGCCGACGTTACGCTTGGCGGCGGAGCAAAAACCTTTTCTGAAACGGCAACCGCTGTTGAATGGCAGGGAAAAACGCTGCTTGAACAGGCACAGACGCTTGGTTATCAGtt                                                                                                                                   >  1:1958109/1‑150 (MQ=255)
              cGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGtt                                                                                                                                   <  1:732704/150‑1 (MQ=255)
               ggAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTg                                                                                                                                  >  1:2354805/1‑150 (MQ=255)
               ggAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTg                                                                                                                                  >  2:5085440/1‑150 (MQ=255)
                 aaaaGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGt                                                                                                                                <  2:517328/150‑1 (MQ=255)
                 aaaaGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGt                                                                                                                                >  1:1237351/1‑150 (MQ=255)
                  aaaGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTg                                                                                                                               >  1:3015861/1‑150 (MQ=255)
                     ggATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGc                                                                                                                            >  1:101288/1‑150 (MQ=255)
                        tCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAt                                                                                                                         >  2:3931373/1‑150 (MQ=255)
                          gATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGAtgc                                                                                                                       >  2:3277265/1‑150 (MQ=255)
                           aTTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGTGCGAtgct                                                                                                                      <  2:5303702/150‑1 (MQ=255)
                           aTTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtgct                                                                                                                      >  2:3816961/1‑150 (MQ=255)
                           aTTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtgct                                                                                                                      <  1:3872501/150‑1 (MQ=255)
                           aTTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAtgct                                                                                                                      <  1:1822962/150‑1 (MQ=255)
                               ccGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCt                                                                                                                  >  1:1459851/1‑150 (MQ=255)
                                cGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTc                                                                                                                 >  1:815596/1‑150 (MQ=255)
                                 gAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCa                                                                                                                >  2:4653780/1‑150 (MQ=255)
                                  aaCAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCATTGATGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCAc                                                                                                               >  1:3796948/1‑150 (MQ=255)
                                   aCAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACt                                                                                                              <  1:153201/150‑1 (MQ=255)
                                     aGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGa                                                                                                            <  2:2030259/150‑1 (MQ=255)
                                       ctgctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAAt                                                                                                          >  2:2637251/1‑150 (MQ=255)
                                        tgctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAAtt                                                                                                         <  1:2248270/150‑1 (MQ=255)
                                        tgctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAAtt                                                                                                         >  2:5256815/1‑150 (MQ=255)
                                         gtttAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTc                                                                                                        <  1:1917482/148‑1 (MQ=255)
                                         gctTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTc                                                                                                        <  2:3652787/150‑1 (MQ=255)
                                            tAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGt                                                                                                     >  1:32081/1‑150 (MQ=255)
                                             aaCGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTg                                                                                                    <  2:2532943/150‑1 (MQ=255)
                                              aCGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGa                                                                                                   <  2:1073408/150‑1 (MQ=255)
                                                gCTCGTGCCGACGTTACGCTTGGCGGTGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGTTGCCTCACTGAATTCGGTGACg                                                                                                 <  2:241461/150‑1 (MQ=255)
                                                    gTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAg                                                                                             <  1:2093182/150‑1 (MQ=255)
                                                        cGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGaa                                                                                         <  2:893558/150‑1 (MQ=255)
                                                        cGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGaa                                                                                         >  1:2453696/1‑150 (MQ=255)
                                                          aCGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATc                                                                                       <  2:4623621/150‑1 (MQ=255)
                                                            gTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAg                                                                                     <  1:4389042/150‑1 (MQ=255)
                                                            gTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAg                                                                                     >  2:1270207/1‑150 (MQ=255)
                                                              tACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCa                                                                                   >  1:2045014/1‑150 (MQ=255)
                                                              tACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCa                                                                                   <  2:4339719/150‑1 (MQ=255)
                                                                cGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACTGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaa                                                                                 <  2:37184/150‑1 (MQ=255)
                                                                cGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCaaa                                                                                 <  1:4572388/150‑1 (MQ=255)
                                                                    tGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAAcc                                                                             >  1:167130/1‑150 (MQ=255)
                                                                    tGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAAcc                                                                             >  1:5328023/1‑150 (MQ=255)
                                                                        ggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTg                                                                         <  1:3618590/150‑1 (MQ=255)
                                                                        ggcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTg                                                                         >  2:169065/1‑150 (MQ=255)
                                                                         gcggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGACGCGAATCAGCAAAAACCCCTGc                                                                        >  2:1805783/1‑150 (MQ=255)
                                                                          cggcgCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACAGAATTCGGTGACGGAAGAGAATCAGCAAAAACCCCTGCt                                                                       >  2:4037336/1‑150 (MQ=255)
                                                                            gcgcAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTg                                                                     >  1:421980/1‑150 (MQ=255)
                                                                            gcgcAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTg                                                                     >  2:5203470/1‑150 (MQ=255)
                                                                            gcgcAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTg                                                                     <  1:402594/150‑1 (MQ=255)
                                                                                 aaaaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTg                                                                >  2:556862/1‑150 (MQ=255)
                                                                                 aaaaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTg                                                                >  2:2376275/1‑150 (MQ=255)
                                                                                  aaaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGt                                                               >  2:3157688/1‑150 (MQ=255)
                                                                                   aaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAACACCCCTGCTTGGCCTGtt                                                              <  2:3451309/150‑1 (MQ=255)
                                                                                   aaCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGtt                                                              >  1:1418015/1‑150 (MQ=255)
                                                                                    aCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGttt                                                             <  1:5021397/150‑1 (MQ=255)
                                                                                     ccTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTg                                                            >  2:3538857/1‑150 (MQ=255)
                                                                                     ccTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTg                                                            <  1:3305162/150‑1 (MQ=255)
                                                                                      cTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGc                                                           >  2:1945027/1‑150 (MQ=255)
                                                                                      cTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGc                                                           >  2:2877450/1‑150 (MQ=255)
                                                                                         tGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGa                                                        <  1:3129226/150‑1 (MQ=255)
                                                                                            tGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACgg                                                     <  2:4506092/150‑1 (MQ=255)
                                                                                               aaCGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCaa                                                  >  2:2746389/1‑150 (MQ=255)
                                                                                               aaCGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCaa                                                  <  2:2542736/150‑1 (MQ=255)
                                                                                                aCGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAat                                                 >  2:4892238/1‑150 (MQ=255)
                                                                                                    cAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGc                                             >  1:2383367/1‑150 (MQ=255)
                                                                                                         gCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTg                                        <  1:3912697/150‑1 (MQ=255)
                                                                                                             gTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCt                                    <  2:619225/150‑1 (MQ=255)
                                                                                                               gAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTgg                                  >  1:3669724/1‑150 (MQ=255)
                                                                                                                aaTGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGc                                 >  2:1218016/1‑150 (MQ=255)
                                                                                                                      aGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGAc                           >  1:3316184/1‑150 (MQ=255)
                                                                                                                       gggAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGAcc                          <  1:349559/150‑1 (MQ=255)
                                                                                                                         gAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGa                        <  2:2924667/150‑1 (MQ=255)
                                                                                                                          aaaaaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGaa                       >  2:4422619/1‑150 (MQ=255)
                                                                                                                           aaaaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGaaa                      >  1:5135633/1‑150 (MQ=255)
                                                                                                                           aaaaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGaaa                      >  2:2682529/1‑150 (MQ=255)
                                                                                                                            aaaCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAg                     <  2:2129320/150‑1 (MQ=255)
                                                                                                                              aCGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAGTATGCCAGTGCGCTGGCTAGGACCGAAAGCa                   <  2:4998414/150‑1 (MQ=255)
                                                                                                                               cGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCaa                  <  2:3421252/150‑1 (MQ=255)
                                                                                                                                 cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                <  2:4937361/150‑1 (MQ=255)
                                                                                                                                 cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                >  1:2635084/1‑150 (MQ=255)
                                                                                                                                 cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                <  2:4942845/150‑1 (MQ=255)
                                                                                                                                 cTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACg                >  1:933926/1‑150 (MQ=255)
                                                                                                                                     gTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCATGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTAcc            >  2:2391373/1‑150 (MQ=255)
                                                                                                                                      tGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCa           <  1:3439930/150‑1 (MQ=255)
                                                                                                                                        aaCAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATg         <  2:3034587/150‑1 (MQ=255)
                                                                                                                                           aggcacagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCa      <  2:281631/150‑1 (MQ=255)
                                                                                                                                               acagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAatat  <  1:278899/150‑1 (MQ=255)
                                                                                                                                               acagacGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAatat  <  1:2936515/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATAT  >  CP009273/397812‑398104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCG  >  CP009273/397810‑398106
                                                                                                                                                     |                                                                                                                                                   
ACGCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGA                                                                                                                                                     >  SRR19973062.1190506/1‑150 (MQ=60)
 CGCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGAC                                                                                                                                                    <  SRR19973062.5347160/150‑1 (MQ=60)
   CTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTAAACGCTCGTGCCGACGTTACTCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGC                                                                                                                                                  >  SRR19973062.1187422/1‑150 (MQ=60)
           AAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTAT                                                                                                                                          <  SRR19973062.1143030/150‑1 (MQ=60)
           AAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTAT                                                                                                                                          <  SRR19973062.3802812/150‑1 (MQ=60)
           AAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTAC                                                                                                                                          <  SRR19973062.1143009/150‑1 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGACCAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATC                                                                                                                                         <  SRR19973062.133152/150‑1 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATC                                                                                                                                         >  SRR19973062.1554012/1‑150 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATC                                                                                                                                         >  SRR19973062.823953/1‑150 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATC                                                                                                                                         >  SRR19973062.4968732/1‑150 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATC                                                                                                                                         >  SRR19973062.4963214/1‑150 (MQ=60)
            AAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATC                                                                                                                                         >  SRR19973062.4963185/1‑150 (MQ=60)
             AGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCA                                                                                                                                        <  SRR19973062.3016222/150‑1 (MQ=60)
             AGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCA                                                                                                                                        <  SRR19973062.3842511/150‑1 (MQ=60)
             AGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCA                                                                                                                                        >  SRR19973062.1073680/1‑150 (MQ=60)
              GGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAG                                                                                                                                       <  SRR19973062.2059924/150‑1 (MQ=60)
                CGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCTTGCCGACGTTACGCTTGGCGGCGGAGCAAAAACCTTTTCTGAAACGGCAACCGCTGTTGAATGGCAGGGAAAAACGCTGCTTGAACAGGCACAGACGCTTGGTTATCAGTT                                                                                                                                     >  SRR19973062.1968177/1‑150 (MQ=60)
                CGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTT                                                                                                                                     <  SRR19973062.736035/150‑1 (MQ=60)
                CGGAAAAGGATCGATTACCGAACAGCGGCTTAACGCTCGCGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTT                                                                                                                                     <  SRR19973062.2980929/150‑1 (MQ=60)
                 GGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTG                                                                                                                                    >  SRR19973062.2367356/1‑150 (MQ=60)
                  GAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGG                                                                                                                                   >  SRR19973062.2380692/1‑150 (MQ=60)
                   AAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGT                                                                                                                                  >  SRR19973062.3198254/1‑150 (MQ=60)
                   AAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGT                                                                                                                                  >  SRR19973062.1243086/1‑150 (MQ=60)
                    AAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTG                                                                                                                                 >  SRR19973062.3031616/1‑150 (MQ=60)
                    AAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTG                                                                                                                                 <  SRR19973062.2059900/150‑1 (MQ=60)
                      AGGATCGATTACCGAACAGCTGCTTAACTCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAG                                                                                                                               >  SRR19973062.415777/1‑150 (MQ=60)
                      AGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAG                                                                                                                               >  SRR19973062.853975/1‑150 (MQ=60)
                       GGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGC                                                                                                                              <  SRR19973062.3895934/150‑1 (MQ=60)
                       GGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGC                                                                                                                              <  SRR19973062.2360693/150‑1 (MQ=60)
                       GGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGC                                                                                                                              >  SRR19973062.101944/1‑150 (MQ=60)
                          TCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGAT                                                                                                                           <  SRR19973062.770921/150‑1 (MQ=60)
                           CGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAACCCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATG                                                                                                                          >  SRR19973062.202703/1‑150 (MQ=60)
                           CGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATG                                                                                                                          <  SRR19973062.3320464/150‑1 (MQ=60)
                            GATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGC                                                                                                                         <  SRR19973062.5108026/150‑1 (MQ=60)
                             ATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCT                                                                                                                        <  SRR19973062.3892264/150‑1 (MQ=60)
                             ATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCT                                                                                                                        <  SRR19973062.1831952/150‑1 (MQ=60)
                              TTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTG                                                                                                                       >  SRR19973062.2684015/1‑150 (MQ=60)
                              TTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTG                                                                                                                       <  SRR19973062.3489285/150‑1 (MQ=60)
                              TGACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTG                                                                                                                       <  SRR19973062.1645774/150‑1 (MQ=60)
                               TACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGC                                                                                                                      >  SRR19973062.1261683/1‑150 (MQ=60)
                                ACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCC                                                                                                                     <  SRR19973062.3624076/150‑1 (MQ=60)
                                 CCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCT                                                                                                                    >  SRR19973062.1466988/1‑150 (MQ=60)
                                 CCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCT                                                                                                                    >  SRR19973062.2645652/1‑150 (MQ=60)
                                  CGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTC                                                                                                                   >  SRR19973062.819201/1‑150 (MQ=60)
                                   GAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCA                                                                                                                  <  SRR19973062.1717745/150‑1 (MQ=60)
                                   GAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCA                                                                                                                  >  SRR19973062.4560119/1‑150 (MQ=60)
                                    AACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCATTGATGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCAC                                                                                                                 >  SRR19973062.3816386/1‑150 (MQ=60)
                                     ACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACT                                                                                                                <  SRR19973062.154081/150‑1 (MQ=60)
                                      CAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTG                                                                                                               >  SRR19973062.531530/1‑150 (MQ=60)
                                          TGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATT                                                                                                           <  SRR19973062.2259489/150‑1 (MQ=60)
                                           GTTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTC                                                                                                          <  SRR19973062.1927302/150‑1 (MQ=60)
                                            CTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCG                                                                                                         >  SRR19973062.1359967/1‑150 (MQ=60)
                                              TAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGT                                                                                                       >  SRR19973062.32311/1‑150 (MQ=60)
                                               AACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTG                                                                                                      >  SRR19973062.3984611/1‑150 (MQ=60)
                                               AACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTG                                                                                                      <  SRR19973062.2534431/150‑1 (MQ=60)
                                                 CGCTCGTGCCGTCGTGACGCTGGGCGGCGGCGCAACAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGGAGGGATAACCGCTGCGTGAACAGGCACAGACGCGGGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGAC                                                                                                    <  SRR19973062.3768539/150‑1 (MQ=60)
                                                  GCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGACAAACGCTGCGTGAACAGGCACAGACGCGAGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACG                                                                                                   >  SRR19973062.1377613/1‑150 (MQ=60)
                                                      GTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAG                                                                                               <  SRR19973062.2103877/150‑1 (MQ=60)
                                                      GTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAG                                                                                               <  SRR19973062.4896262/150‑1 (MQ=60)
                                                       TGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGC                                                                                              <  SRR19973062.1780918/150‑1 (MQ=60)
                                                          CGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAA                                                                                           >  SRR19973062.2466867/1‑150 (MQ=60)
                                                          CGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAA                                                                                           >  SRR19973062.2466745/1‑150 (MQ=60)
                                                           GACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAAT                                                                                          <  SRR19973062.1917921/150‑1 (MQ=60)
                                                            ACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATC                                                                                         >  SRR19973062.3688059/1‑150 (MQ=60)
                                                              GTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAG                                                                                       <  SRR19973062.4521544/150‑1 (MQ=60)
                                                              GTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAG                                                                                       >  SRR19973062.3388973/1‑150 (MQ=60)
                                                              GTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAG                                                                                       <  SRR19973062.4411529/150‑1 (MQ=60)
                                                                TACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCA                                                                                     >  SRR19973062.3246181/1‑150 (MQ=60)
                                                                TACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCA                                                                                     >  SRR19973062.2055411/1‑150 (MQ=60)
                                                                TACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCA                                                                                     >  SRR19973062.2055500/1‑150 (MQ=60)
                                                                  CGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAA                                                                                   <  SRR19973062.4596282/150‑1 (MQ=60)
                                                                      TGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACC                                                                               >  SRR19973062.5355972/1‑150 (MQ=60)
                                                                      TGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACC                                                                               >  SRR19973062.168061/1‑150 (MQ=60)
                                                                          GGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTG                                                                           <  SRR19973062.3637056/150‑1 (MQ=60)
                                                                          GGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTG                                                                           >  SRR19973062.5024265/1‑150 (MQ=60)
                                                                             GGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTT                                                                        <  SRR19973062.2326155/150‑1 (MQ=60)
                                                                              GCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTG                                                                       >  SRR19973062.423797/1‑150 (MQ=60)
                                                                              GCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTG                                                                       <  SRR19973062.404367/150‑1 (MQ=60)
                                                                                  AAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCT                                                                   >  SRR19973062.5249546/1‑150 (MQ=60)
                                                                                    AAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGT                                                                 <  SRR19973062.3545980/150‑1 (MQ=60)
                                                                                    AAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGT                                                                 >  SRR19973062.3196371/1‑150 (MQ=60)
                                                                                    AAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGT                                                                 >  SRR19973062.504761/1‑150 (MQ=60)
                                                                                     AACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTT                                                                >  SRR19973062.1424957/1‑150 (MQ=60)
                                                                                     AACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTT                                                                >  SRR19973062.1424831/1‑150 (MQ=60)
                                                                                      ACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTT                                                               <  SRR19973062.5047984/150‑1 (MQ=60)
                                                                                       CCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTG                                                              <  SRR19973062.3322263/150‑1 (MQ=60)
                                                                                       CCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTG                                                              <  SRR19973062.3322327/150‑1 (MQ=60)
                                                                                        CTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGC                                                             >  SRR19973062.3067785/1‑150 (MQ=60)
                                                                                           TGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGA                                                          <  SRR19973062.3145332/150‑1 (MQ=60)
                                                                                            GCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGAC                                                         >  SRR19973062.3358763/1‑150 (MQ=60)
                                                                                              TGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGG                                                       >  SRR19973062.1197309/1‑150 (MQ=60)
                                                                                              TGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGG                                                       >  SRR19973062.1197139/1‑150 (MQ=60)
                                                                                               GAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGC                                                      >  SRR19973062.1930502/1‑150 (MQ=60)
                                                                                                    GGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATAT                                                 >  SRR19973062.4283084/1‑150 (MQ=60)
                                                                                                      CAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGC                                               >  SRR19973062.2396142/1‑150 (MQ=60)
                                                                                                      CAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGC                                               >  SRR19973062.2396146/1‑150 (MQ=60)
                                                                                                         CCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAG                                            <  SRR19973062.215447/150‑1 (MQ=60)
                                                                                                          CGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGT                                           <  SRR19973062.3863454/150‑1 (MQ=60)
                                                                                                           GCTGGTGAATGGCAGGGAAAATAGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGATCGATGCTGCCACACTGAAATCGGTGAAGGAAGCTAATCAGCAAAAACAACAGCTTGGCCTGTTTGCAGACTGAAATATGCCAGAG                                          >  SRR19973062.1535662/1‑150 (MQ=60)
                                                                                                           GCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTG                                          <  SRR19973062.3932654/150‑1 (MQ=60)
                                                                                                           GCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTG                                          <  SRR19973062.3932621/150‑1 (MQ=60)
                                                                                                             TGGTGAATGGCAGGGAAAAACTCTGCGTGAACAGGCACAGAAGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCG                                        >  SRR19973062.1478052/1‑150 (MQ=60)
                                                                                                                 GAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGG                                    >  SRR19973062.102065/1‑150 (MQ=60)
                                                                                                                 GAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGG                                    >  SRR19973062.3688542/1‑150 (MQ=60)
                                                                                                                 GAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGG                                    >  SRR19973062.4750751/1‑150 (MQ=60)
                                                                                                                    TGGCAGGGAAACACGCTGCGTGAACAGGCACAGTCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCTGCAAACACCCCTGATTGGCCTGTTTGCTGACGGCTATATGCCAGTGCGCTGGCTA                                 <  SRR19973062.4400093/150‑1 (MQ=60)
                                                                                                                    TGGCAGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTA                                 >  SRR19973062.3681762/1‑150 (MQ=60)
                                                                                                                        AGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGAC                             >  SRR19973062.3333329/1‑150 (MQ=60)
                                                                                                                        AGGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGAC                             >  SRR19973062.2206989/1‑150 (MQ=60)
                                                                                                                         GGGAAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACC                            <  SRR19973062.351153/150‑1 (MQ=60)
                                                                                                                            AAAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAA                         >  SRR19973062.4166801/1‑150 (MQ=60)
                                                                                                                             AAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAA                        >  SRR19973062.5162796/1‑150 (MQ=60)
                                                                                                                             AAAACGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAA                        >  SRR19973062.2843188/1‑150 (MQ=60)
                                                                                                                                 CGCTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAA                    >  SRR19973062.1850722/1‑150 (MQ=60)
                                                                                                                                   CTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACG                  >  SRR19973062.938114/1‑150 (MQ=60)
                                                                                                                                   CTGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACG                  >  SRR19973062.2648963/1‑150 (MQ=60)
                                                                                                                                    TGCGTGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGT                 <  SRR19973062.3325931/150‑1 (MQ=60)
                                                                                                                                        TGAACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCA             <  SRR19973062.3457505/150‑1 (MQ=60)
                                                                                                                                           ACAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGACAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGG          >  SRR19973062.1290369/1‑150 (MQ=60)
                                                                                                                                            CAGGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGC         >  SRR19973062.599699/1‑150 (MQ=60)
                                                                                                                                              GGCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAA       >  SRR19973062.562013/1‑150 (MQ=60)
                                                                                                                                               GCACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAAT      <  SRR19973062.2929394/150‑1 (MQ=60)
                                                                                                                                                 ACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATAT    <  SRR19973062.280270/150‑1 (MQ=60)
                                                                                                                                                 ACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATAT    <  SRR19973062.280215/150‑1 (MQ=60)
                                                                                                                                                 ACAGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATAT    <  SRR19973062.2951968/150‑1 (MQ=60)
                                                                                                                                                   AGACGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCG  <  SRR19973062.5284485/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ACGCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCG  >  CP009273/397810‑398106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑