Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,642,051	T→G	29.6%	A19A (GCA→GCC)	dicC ←	Qin prophage; DNA‑binding transcriptional regulator for DicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,642,051	0	T	G	29.6%	374.0 / 313.7	337	A19A (GCA→GCC)	dicC	Qin prophage; DNA‑binding transcriptional regulator for DicB
Reads supporting (aligned to +/- strand): ref base T (94/143); new base G (46/54); total (140/197)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

CGCCCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAA  >  CP009273/1641902‑1642200

cgccccgcccGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACctgct                                                                                                                                                       >  2:1724836/1‑150 (MQ=255)

  ccccgcccGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCtgcggc                                                                                                                                                     <  2:4909598/150‑1 (MQ=255)

    ccgcccgcTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCtt                                                                                                                                                   <  1:79472/150‑1 (MQ=255)

              cGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAgttt                                                                                                                                         >  2:2977706/1‑150 (MQ=255)

                 cTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACATGCGGCTTGTGCAAgttttgt                                                                                                                                      >  1:3679402/1‑150 (MQ=255)

                     cGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCTAGTTTTGttttt                                                                                                                                  <  2:1982475/150‑1 (MQ=255)

                       atatTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGa                                                                                                                                >  1:3740615/1‑150 (MQ=255)

                       atatTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGa                                                                                                                                >  1:916384/1‑150 (MQ=255)

                         atTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAAc                                                                                                                              >  2:1675687/1‑150 (MQ=255)

                         atTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAAc                                                                                                                              <  2:3279517/150‑1 (MQ=255)

                         atTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAAc                                                                                                                              >  1:3484465/1‑150 (MQ=255)

                             atcatAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGaa                                                                                                                          >  2:5006024/1‑150 (MQ=255)

                             atcatAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGaa                                                                                                                          >  2:3529794/1‑150 (MQ=255)

                               catAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAAt                                                                                                                        <  1:2163349/150‑1 (MQ=255)

                                   aaCTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACaa                                                                                                                    >  1:4635032/1‑150 (MQ=255)

                                    aCTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACaaa                                                                                                                   <  2:3344842/150‑1 (MQ=255)

                                      tttGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAag                                                                                                                 >  1:3941660/1‑150 (MQ=255)

                                       ttGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAACTCCCCTTCCCAGCTATACAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAACTACAAAaga                                                                                                                <  2:1316194/150‑1 (MQ=255)

                                        tGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAagag                                                                                                               <  1:2867292/150‑1 (MQ=255)

                                         gggATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGc                                                                                                              <  2:1257683/150‑1 (MQ=255)

                                          ggATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCg                                                                                                             >  2:539337/1‑150 (MQ=255)

                                            aTCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTc                                                                                                           >  2:1208910/1‑150 (MQ=255)

                                              cATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAg                                                                                                         >  2:1767101/1‑150 (MQ=255)

                                                tACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGtt                                                                                                       <  2:806721/150‑1 (MQ=255)

                                                 aCTGAAGCTCACCGCCAGATGCCTCCTGTAGAAGCATCGCGAGACCTTCGGGAACTATATCCCCTTTCCAGCTATACAGCGAAGCCAACCGACTACCTGAGGCTTGTTCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGttt                                                                                                      <  2:1894150/150‑1 (MQ=255)

                                                  cTGAAGCTCCCNGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGtttt                                                                                                     >  1:2764552/1‑150 (MQ=255)

                                                  cTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGtttt                                                                                                     <  1:4983160/150‑1 (MQ=255)

                                                  cTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGtttt                                                                                                     >  1:2304195/1‑150 (MQ=255)

                                                  cTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGtttt                                                                                                     <  2:4343290/150‑1 (MQ=255)

                                                     aaGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAg                                                                                                  <  2:3206098/150‑1 (MQ=255)

                                                     aaGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAg                                                                                                  <  2:94449/150‑1 (MQ=255)

                                                          ccccGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCAttt                                                                                             <  2:690605/150‑1 (MQ=255)

                                                            ccGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTaa                                                                                           <  1:2512310/150‑1 (MQ=255)

                                                             cGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTaaa                                                                                          >  2:1077167/1‑150 (MQ=255)

                                                             cGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTaaa                                                                                          >  1:4003259/1‑150 (MQ=255)

                                                             cGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTaaa                                                                                          <  2:290902/150‑1 (MQ=255)

                                                                     gCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCttt                                                                                  <  1:5067482/150‑1 (MQ=255)

                                                                      cctcctGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTa                                                                                 >  1:4920808/1‑150 (MQ=255)

                                                                        tcctGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTAtt                                                                               <  1:2595345/150‑1 (MQ=255)

                                                                           tGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGtt                                                                            <  2:2291026/150‑1 (MQ=255)

                                                                           tGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGtt                                                                            <  1:5188510/150‑1 (MQ=255)

                                                                             tAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCAAAACGAATACCTGCTTCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAg                                                                          <  2:4291073/150‑1 (MQ=255)

                                                                              aGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGt                                                                         <  2:817106/150‑1 (MQ=255)

                                                                              aGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGt                                                                         <  2:4306985/150‑1 (MQ=255)

                                                                              aGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGt                                                                         <  1:1567859/150‑1 (MQ=255)

                                                                                 cGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAt                                                                      <  2:1393931/150‑1 (MQ=255)

                                                                                  gCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAta                                                                     <  1:190385/150‑1 (MQ=255)

                                                                                  gCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAta                                                                     <  1:3522519/150‑1 (MQ=255)

                                                                                    aTCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaat                                                                   <  2:4777027/150‑2 (MQ=255)

                                                                                    aTCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaat                                                                   >  1:1880795/1‑149 (MQ=255)

                                                                                    aTCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaat                                                                   <  2:3236097/150‑2 (MQ=255)

                                                                                     tCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATACAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaatt                                                                  <  2:5258645/150‑3 (MQ=255)

                                                                                     tCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaatt                                                                  <  1:3100294/150‑3 (MQ=255)

                                                                                     tCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaatt                                                                  <  1:137418/150‑3 (MQ=255)

                                                                                      cgcgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaatta                                                                 <  2:1537174/150‑1 (MQ=255)

                                                                                      cgcgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaatta                                                                 <  2:421354/150‑1 (MQ=255)

                                                                                       gcgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaattaa                                                                <  1:2070670/150‑1 (MQ=255)

                                                                                          cgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACaa                                                             >  2:3843160/1‑150 (MQ=255)

                                                                                           gACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAag                                                            <  2:211408/150‑1 (MQ=255)

                                                                                                 cGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAagatagat                                                      <  1:592180/150‑1 (MQ=255)

                                                                                                  gggAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGTAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATg                                                     >  1:1248457/1‑150 (MQ=255)

                                                                                                  gggAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATg                                                     >  2:2636304/1‑150 (MQ=255)

                                                                                                    gAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGtt                                                   <  1:2810779/150‑1 (MQ=255)

                                                                                                       cTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAAc                                                <  1:1012548/150‑1 (MQ=255)

                                                                                                        tAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACa                                               <  1:1690590/150‑1 (MQ=255)

                                                                                                         aaaTCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACaa                                              >  1:404484/1‑150 (MQ=255)

                                                                                                           aTCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACaaaa                                            >  2:4247994/1‑150 (MQ=255)

                                                                                                             ccccTTTCCAGCTATAAAGCGACGCCAAACGAATACCTGCTGCTGGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTCAGCATGTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAAc                                          >  2:31175/1‑150 (MQ=255)

                                                                                                             ccccTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAAc                                          >  1:693428/1‑150 (MQ=255)

                                                                                                                 tttCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAg                                      <  1:4380890/150‑1 (MQ=255)

                                                                                                                    ccAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCa                                   <  1:788127/150‑1 (MQ=255)

                                                                                                                      aGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAAt                                 <  2:3942739/150‑1 (MQ=255)

                                                                                                                      aGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAAt                                 <  2:5115511/150‑1 (MQ=255)

                                                                                                                      aGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAAt                                 <  2:3407177/150‑1 (MQ=255)

                                                                                                                       gCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATa                                >  1:3402028/1‑150 (MQ=255)

                                                                                                                       gCTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATa                                <  1:2655680/150‑1 (MQ=255)

                                                                                                                        cTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATAc                               >  1:433592/1‑150 (MQ=255)

                                                                                                                        cTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATAc                               <  1:3690245/150‑1 (MQ=255)

                                                                                                                        cTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATATTCAATAc                               >  1:4775179/1‑150 (MQ=255)

                                                                                                                        cTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATAc                               <  2:682549/150‑1 (MQ=255)

                                                                                                                        cTATAAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGAGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATAc                               <  2:3679216/150‑1 (MQ=255)

                                                                                                                           taAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGAt                            >  2:4026763/1‑150 (MQ=255)

                                                                                                                           taAAGCGAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGAt                            <  1:2222425/150‑1 (MQ=255)

                                                                                                                            aaaGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGAtt                           >  1:335434/1‑150 (MQ=255)

                                                                                                                              aGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTCAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTa                         >  1:4656881/1‑150 (MQ=255)

                                                                                                                               gCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTG‑TTTTGAACAGAAAAACAAAAGAGCGTCAGTTTTAAGCATATAAAACACCTTTATTTTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATAAGATTTAga                       >  1:3794722/1‑149 (MQ=255)

                                                                                                                                 gAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCa                      >  1:3206626/1‑150 (MQ=255)

                                                                                                                                 gAAGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAAATAGATGTTAACAAAAACATAGTCAATACGATTTAGCa                      >  1:2020713/1‑150 (MQ=255)

                                                                                                                                  aaGCCAAACGAATACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCAt                     <  1:2987420/150‑1 (MQ=255)

                                                                                                                                   aGCCAAACGACTTCCTGCTGCTTGTGCTAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAATCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCTATACGATTTAGCAtt                    <  1:1724836/150‑1 (MQ=255)

                                                                                                                                       aaaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCt                <  2:3833115/150‑1 (MQ=255)

                                                                                                                                       aaaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCt                >  1:1133560/1‑150 (MQ=255)

                                                                                                                                       aaaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCt                >  2:2477034/1‑150 (MQ=255)

                                                                                                                                           gAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACt            <  2:2764175/150‑1 (MQ=255)

                                                                                                                                            aaTACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTa           >  1:4187266/1‑150 (MQ=255)

                                                                                                                                             aTACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTAt          >  1:4887024/1‑150 (MQ=255)

                                                                                                                                              tACCTGCGGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATg         <  2:910183/150‑1 (MQ=255)

                                                                                                                                               aCCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATgg        <  2:4437735/150‑1 (MQ=255)

                                                                                                                                                  tgcggcTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGaaa     >  1:3665606/1‑150 (MQ=255)

                                                                                                                                                   gctgctTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAATTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAAc    <  2:3740615/150‑1 (MQ=255)

CGCCCCGCCCGCTTCGTCTTACGATATTCATCATAAACTTTGGGATCATACTGAAGCTCCCCGCCAGATGCCTCCTGTAGACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAA  >  CP009273/1641902‑1642200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A