Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,787,242 C→A 18.0% D192Y (GAT→TAT)  btuD ← vitamin B12 transporter subunit : ATP‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,787,2420CA18.0% 923.1 / 125.4 327D192Y (GAT→TAT) btuDvitamin B12 transporter subunit : ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (114/154);  new base A (29/30);  total (143/184)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 335 total aligned reads displayed.

GACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCA  >  CP009273/1787092‑1787391
                                                                                                                                                      |                                                                                                                                                     
 aCCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCTCCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATc                                                                                                                                                       <  2:4840410/150‑1 (MQ=255)
  ccTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCg                                                                                                                                                      >  1:5112630/1‑150 (MQ=255)
    ttCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCCCGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTg                                                                                                                                                    <  2:389941/150‑1 (MQ=255)
       gATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACt                                                                                                                                                 <  1:3865071/150‑1 (MQ=255)
       gATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACt                                                                                                                                                 >  2:1398233/1‑150 (MQ=255)
       gATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACAACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACt                                                                                                                                                 >  2:250053/1‑150 (MQ=255)
        atatCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTg                                                                                                                                                >  1:388097/1‑150 (MQ=255)
          atCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCt                                                                                                                                              <  1:242307/150‑1 (MQ=255)
             cAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCtcat                                                                                                                                           <  2:2563669/150‑1 (MQ=255)
             cAGACGACGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCtcat                                                                                                                                           <  1:4545101/150‑1 (MQ=255)
                aCGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATcac                                                                                                                                        <  2:4735938/150‑1 (MQ=255)
                aCGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATcac                                                                                                                                        <  2:1258789/150‑1 (MQ=255)
                 cGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATcaca                                                                                                                                       <  2:5252408/150‑1 (MQ=255)
                  ggCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACaa                                                                                                                                      >  2:2459840/1‑150 (MQ=255)
                   gCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAAt                                                                                                                                     <  1:3768569/150‑1 (MQ=255)
                       aaaTTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGcc                                                                                                                                 >  2:3168766/1‑150 (MQ=255)
                       aaaTTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGcc                                                                                                                                 <  1:4122445/150‑1 (MQ=255)
                       aaaTTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGcc                                                                                                                                 <  2:1063558/150‑1 (MQ=255)
                             aTCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCt                                                                                                                           >  1:2225417/1‑150 (MQ=255)
                              tCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCtt                                                                                                                          >  2:181614/1‑150 (MQ=255)
                               cccATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTg                                                                                                                         <  2:634884/150‑1 (MQ=255)
                                 cATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCt                                                                                                                       >  1:2942277/1‑150 (MQ=255)
                                 cATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCt                                                                                                                       <  1:5044413/150‑1 (MQ=255)
                                 cATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCt                                                                                                                       >  2:879142/1‑150 (MQ=255)
                                  aTAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTg                                                                                                                      >  2:66382/1‑150 (MQ=255)
                                   tAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGa                                                                                                                     >  2:3081354/1‑150 (MQ=255)
                                     ggCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGaca                                                                                                                   >  2:4394784/1‑150 (MQ=255)
                                         tGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAgc                                                                                                               <  1:171561/150‑1 (MQ=255)
                                            gcCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAgcgcg                                                                                                            <  1:894483/150‑1 (MQ=255)
                                            gcCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAgcgcg                                                                                                            >  1:4589565/1‑150 (MQ=255)
                                               aGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTc                                                                                                         <  2:272050/150‑1 (MQ=255)
                                               aGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTc                                                                                                         >  2:4234737/1‑150 (MQ=255)
                                                   ttGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTCCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGaa                                                                                                     <  2:2590819/150‑1 (MQ=255)
                                                     ggcggcGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAAtt                                                                                                   <  1:2139366/150‑1 (MQ=255)
                                                      gcggcgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAAttt                                                                                                  <  2:4570921/150‑1 (MQ=255)
                                                      gcggcgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAAttt                                                                                                  <  1:3421636/150‑1 (MQ=255)
                                                        ggcgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTg                                                                                                <  1:945753/150‑1 (MQ=255)
                                                         gcgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCCTGCTGACACAGCGCGCTCAGAATTTTGt                                                                                               <  2:3966269/150‑1 (MQ=255)
                                                         gcgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGt                                                                                               <  1:3318788/150‑1 (MQ=255)
                                                          cgTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTc                                                                                              <  1:1916150/150‑1 (MQ=255)
                                                           gTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCt                                                                                             <  2:3534550/150‑1 (MQ=255)
                                                            tGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTa                                                                                            <  2:3701381/150‑1 (MQ=255)
                                                             gAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTaa                                                                                           >  2:4959450/1‑150 (MQ=255)
                                                               gCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACg                                                                                         >  1:2503109/1‑150 (MQ=255)
                                                                 aCCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCa                                                                                       >  2:1125365/1‑150 (MQ=255)
                                                                 aCCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCa                                                                                       <  1:15209/150‑1 (MQ=255)
                                                                  ccTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCAc                                                                                      >  2:5178532/1‑150 (MQ=255)
                                                                   ctctTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACt                                                                                     <  2:1456334/150‑1 (MQ=255)
                                                                   ctctTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACt                                                                                     >  1:1025977/1‑150 (MQ=255)
                                                                   ctctTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACt                                                                                     <  2:134902/150‑1 (MQ=255)
                                                                     ctTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACttt                                                                                   >  2:2736011/1‑150 (MQ=255)
                                                                      ttCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTttg                                                                                  <  1:4057424/150‑1 (MQ=255)
                                                                        cccTGCGTCCCCTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTttgtt                                                                                >  1:2358327/1‑150 (MQ=255)
                                                                         ccTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTttgttg                                                                               <  1:2769170/150‑1 (MQ=255)
                                                                          cTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTgc                                                                              >  2:1236079/1‑150 (MQ=255)
                                                                              gTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCaa                                                                          >  1:2065120/1‑150 (MQ=255)
                                                                               tCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAAc                                                                         >  1:3599849/1‑150 (MQ=255)
                                                                                ccACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACa                                                                        >  2:2238390/1‑150 (MQ=255)
                                                                                ccACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACa                                                                        >  1:1113401/1‑150 (MQ=255)
                                                                                 cacaGGCCAGCATTTTTCCACCTTATAGCAACCACGCCCGATGCGCATGACGCACTGTGTGGTTGATATCTTGACTGCTCATCACAAACGCCAGTCCATGCTGACACAGCGCGATCAGAATTATGTCTAACTCACTAAGTAGCGCAACAt                                                                       >  2:5294162/1‑150 (MQ=255)
                                                                                 cACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACAt                                                                       >  2:3455074/1‑150 (MQ=255)
                                                                                   cTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCa                                                                     >  2:1600150/1‑150 (MQ=255)
                                                                                   cTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCa                                                                     >  1:1252157/1‑150 (MQ=255)
                                                                                   cTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCa                                                                     >  1:2856394/1‑150 (MQ=255)
                                                                                     ggCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAg                                                                   <  2:3739364/150‑1 (MQ=255)
                                                                                     ggCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAg                                                                   <  2:5262792/150‑1 (MQ=255)
                                                                                       ccAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGAc                                                                 <  1:3158466/150‑1 (MQ=255)
                                                                                       ccAGCATTTGTCCACCTTTTAGCAACCTCGCACGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACATTCGCCAGTCCTTGCTGTCACAGCGCGCTCTGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGAc                                                                 <  1:445120/150‑1 (MQ=255)
                                                                                        cAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACt                                                                <  1:834394/150‑1 (MQ=255)
                                                                                            aTTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTc                                                            <  2:772117/150‑1 (MQ=255)
                                                                                            aTTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTc                                                            <  2:2054341/150‑1 (MQ=255)
                                                                                              ttttCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCAt                                                          <  2:3631330/150‑1 (MQ=255)
                                                                                              ttttCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCAt                                                          <  2:4487113/150‑1 (MQ=255)
                                                                                              ttttCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCAt                                                          >  1:2621519/1‑150 (MQ=255)
                                                                                                   cACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCt                                                     <  1:1766603/150‑1 (MQ=255)
                                                                                                     ccTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCtca                                                   <  1:4736656/150‑1 (MQ=255)
                                                                                                     cattttAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTCACGCACGATGTGGCGCCACATAAAGACTGTTCATCTGCtca                                                   >  2:1538688/3‑150 (MQ=255)
                                                                                                          tAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCaag                                              <  2:3307297/150‑1 (MQ=255)
                                                                                                              aaCCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAagc                                          >  1:1652227/1‑150 (MQ=255)
                                                                                                                ccACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAagcag                                        <  1:3679914/150‑1 (MQ=255)
                                                                                                                  aCGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAgcagca                                      <  2:951113/150‑1 (MQ=255)
                                                                                                                     cccGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAAtt                                   <  2:1706943/150‑1 (MQ=255)
                                                                                                                       cGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTgg                                 >  1:356745/1‑150 (MQ=255)
                                                                                                                       cGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTgg                                 <  2:2925641/150‑1 (MQ=255)
                                                                                                                          tGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCt                              <  1:719011/150‑1 (MQ=255)
                                                                                                                          tGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCt                              <  1:1221081/150‑1 (MQ=255)
                                                                                                                          tGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTGTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCt                              <  1:2396647/150‑1 (MQ=255)
                                                                                                                           gcgcATGACGCATTGTGTGGTTGAGATCGTGTCTGCTCATCAAAATCGCCAGTAATTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTg                             <  1:5275063/150‑1 (MQ=255)
                                                                                                                               aTGACGCAATGTGTGGTTGAGATAGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCggg                         >  1:4835778/1‑150 (MQ=255)
                                                                                                                                  aCGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGAtt                      >  1:4990357/1‑150 (MQ=255)
                                                                                                                                   cGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTg                     <  1:4904931/150‑1 (MQ=255)
                                                                                                                                     cAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGc                   <  2:3612835/150‑1 (MQ=255)
                                                                                                                                      aaTGTGTGGTTGAGATCGTGACTGCTAATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAAAGCACTATGTTGCGCAACATCAAGACTGTTCATCGTCTCATAAATAAGAAGCAATTGGCCTGCGGGATTGGCt                  >  2:3422216/1‑150 (MQ=255)
                                                                                                                                        tgtgtgGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGACTGCGGGATTGGCTtg                >  2:5316466/1‑150 (MQ=255)
                                                                                                                                           gtgGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTgtgg             >  1:2018755/1‑150 (MQ=255)
                                                                                                                                              gTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGgtgt          <  1:2828363/150‑1 (MQ=255)
                                                                                                                                               ttGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGtgtg         >  1:144337/1‑150 (MQ=255)
                                                                                                                                                tGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGa        <  1:1663891/150‑1 (MQ=255)
                                                                                                                                                    aTCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTg    <  1:2459840/150‑1 (MQ=255)
                                                                                                                                                      cGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCa  >  2:4887423/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCACTGGCCAGCATTTTTCCACCTTTTAGCAACCACGCCCGATGCGCATGACGCAATGTGTGGTTGAGATCGTGACTGCTCATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCA  >  CP009273/1787092‑1787391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A