Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,008,669 C→A 28.1% T103T (ACC→ACA)  fliG → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,008,6690CA28.1% 652.3 / 201.1 315T103T (ACC→ACA) fliGflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base C (116/110);  new base A (41/48);  total (157/158)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCT  >  CP009273/2008520‑2008818
                                                                                                                                                     |                                                                                                                                                     
   gtgCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGcc                                                                                                                                                    >  2:3001117/1‑150 (MQ=255)
    tgCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCa                                                                                                                                                   <  1:85074/150‑1 (MQ=255)
     gCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAg                                                                                                                                                  <  2:1575787/150‑1 (MQ=255)
       tGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCg                                                                                                                                                <  1:1238545/150‑1 (MQ=255)
        ggCGGAGTTTGGGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCgg                                                                                                                                               <  2:1961558/150‑1 (MQ=255)
           ggAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGAGGTAt                                                                                                                                            >  1:4144234/1‑150 (MQ=255)
             aGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTg                                                                                                                                          <  2:514914/150‑1 (MQ=255)
              gTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGa                                                                                                                                         >  2:4151500/1‑150 (MQ=255)
                 tGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAAc                                                                                                                                      <  1:4214533/150‑1 (MQ=255)
                  gAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACg                                                                                                                                     >  1:3351101/1‑150 (MQ=255)
                      aagaagCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCa                                                                                                                                 >  1:2051066/1‑150 (MQ=255)
                         aagCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACt                                                                                                                              <  1:1899500/150‑1 (MQ=255)
                                aCAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGa                                                                                                                       >  1:2866275/1‑150 (MQ=255)
                                aCAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGa                                                                                                                       <  1:4455868/150‑1 (MQ=255)
                                   gTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCTCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGcc                                                                                                                    <  1:4620178/150‑1 (MQ=255)
                                   gTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGcc                                                                                                                    >  1:1381511/1‑150 (MQ=255)
                                     ttGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCcac                                                                                                                  >  2:3243123/1‑150 (MQ=255)
                                      tGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCcaca                                                                                                                 <  2:2698185/150‑1 (MQ=255)
                                           cACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAgcg                                                                                                            <  1:2978565/150‑1 (MQ=255)
                                              tGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGcgccg                                                                                                         >  1:3666784/1‑150 (MQ=255)
                                              tGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTGTATGGAGCCACAGAGcgccg                                                                                                         >  2:3087982/1‑150 (MQ=255)
                                               gAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGcgccgc                                                                                                        >  2:5300222/1‑150 (MQ=255)
                                               gAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGcgccgc                                                                                                        >  2:1874313/1‑150 (MQ=255)
                                                  tatCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGa                                                                                                     >  1:5146459/1‑150 (MQ=255)
                                                  tatCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGa                                                                                                     <  1:1700151/150‑1 (MQ=255)
                                                    tCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATc                                                                                                   <  1:401639/150‑1 (MQ=255)
                                                     cAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCt                                                                                                  >  2:2229562/1‑150 (MQ=255)
                                                     cAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCt                                                                                                  <  2:282931/150‑1 (MQ=255)
                                                       aCGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGa                                                                                                >  2:1417048/1‑150 (MQ=255)
                                                         gCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGAtt                                                                                              >  2:367273/1‑150 (MQ=255)
                                                         gCCAACGAGTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGAtt                                                                                              <  2:4287317/150‑1 (MQ=255)
                                                          ccAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTTCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTc                                                                                             >  2:559357/1‑150 (MQ=255)
                                                          ccAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTc                                                                                             <  1:3464650/150‑1 (MQ=255)
                                                           cAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcg                                                                                            >  2:2025129/1‑150 (MQ=255)
                                                            aaCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcgc                                                                                           <  1:389900/150‑1 (MQ=255)
                                                            aaCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcgc                                                                                           <  1:2553505/150‑1 (MQ=255)
                                                            aaCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcgc                                                                                           >  1:4843434/1‑150 (MQ=255)
                                                            aaCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTAAGGAGCCACAGAGCGCCGCCGATCTGATTcgc                                                                                           <  2:1310849/150‑1 (MQ=255)
                                                            aaCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcgc                                                                                           >  1:2831159/1‑150 (MQ=255)
                                                             aCGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTcgcg                                                                                          <  2:3448225/150‑1 (MQ=255)
                                                              cGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGa                                                                                         >  1:3785960/1‑150 (MQ=255)
                                                                aTTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATg                                                                                       >  1:5016942/1‑150 (MQ=255)
                                                                 ttATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCAGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGa                                                                                      >  1:2699115/1‑150 (MQ=255)
                                                                  tATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAg                                                                                     <  2:3490816/150‑1 (MQ=255)
                                                                     cTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCAt                                                                                  <  2:1771934/150‑1 (MQ=255)
                                                                        cgcTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCg                                                                               <  1:1555375/150‑1 (MQ=255)
                                                                          cTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCa                                                                             >  1:4360463/1‑150 (MQ=255)
                                                                             ggTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAAt                                                                          >  2:2791385/1‑150 (MQ=255)
                                                                             ggTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAAt                                                                          <  1:4078071/150‑1 (MQ=255)
                                                                              gTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAAtt                                                                         <  1:2946409/150‑1 (MQ=255)
                                                                                aTTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTAt                                                                       <  2:3330679/150‑1 (MQ=255)
                                                                                aTTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTAt                                                                       >  2:1703728/1‑150 (MQ=255)
                                                                                 ttGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATc                                                                      <  2:4947019/150‑1 (MQ=255)
                                                                                 ttGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATc                                                                      <  1:892569/150‑1 (MQ=255)
                                                                                  tGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCg                                                                     >  1:2594633/1‑150 (MQ=255)
                                                                                    gTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGAGATTCTCGAAACTCGCGCTACCGCCAGCGGTAATGAAACGCTCAACTTTATGGAGCCACATAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCTAATTATCGcc                                                                   >  2:2634267/1‑150 (MQ=255)
                                                                                       aaaGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGccacc                                                                >  1:4491164/1‑150 (MQ=255)
                                                                                           ctctGGGTGAAGAACGTGCCGCCAGCCTGCTGGTAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTc                                                            <  1:3295240/150‑1 (MQ=255)
                                                                                           ctctGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTc                                                            <  2:2526332/150‑1 (MQ=255)
                                                                                           ctctGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTc                                                            <  2:2172835/150‑1 (MQ=255)
                                                                                            tctGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCACATTATCGCCACCATTCt                                                           >  2:1479228/1‑150 (MQ=255)
                                                                                              tGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTgg                                                         >  2:5075564/1‑150 (MQ=255)
                                                                                              tGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTgg                                                         >  2:2429225/1‑150 (MQ=255)
                                                                                                ggTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTg                                                       <  2:4203391/150‑1 (MQ=255)
                                                                                                ggTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTg                                                       <  2:3482751/150‑1 (MQ=255)
                                                                                                   gaagaaCGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCAt                                                    <  2:1104120/150‑1 (MQ=255)
                                                                                                    aagaaCGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATc                                                   >  1:3500978/1‑150 (MQ=255)
                                                                                                         cGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAg                                              <  2:3175814/150‑1 (MQ=255)
                                                                                                          gTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCACATTATCGCCACCATTCTGGTGCATCTGAAgc                                             >  2:119444/1‑150 (MQ=255)
                                                                                                               gccAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGcc                                        <  1:725573/150‑1 (MQ=255)
                                                                                                                ccAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGccc                                       >  1:4313565/1‑150 (MQ=255)
                                                                                                                   gCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAg                                    >  2:4005437/1‑150 (MQ=255)
                                                                                                                   gCCTGCTGGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAg                                    <  2:4360463/150‑1 (MQ=255)
                                                                                                                    ccTGCTGGACGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGGGCATCTGAAGCGCGCCCAAgc                                   >  2:3399033/1‑150 (MQ=255)
                                                                                                                    ccTGCTGGAAGATATTCTCGATTCTCGCGATACCGCCAGCGGTAGTGAAACGCTCAACTTTTTGGAGCCTCAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAATTTATCGCGTCCATTCTGGTGCTTCTGAAGCGCGCCCAAgc                                   <  2:2644750/150‑1 (MQ=255)
                                                                                                                     ctgctgGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAgcc                                  >  1:1161203/1‑150 (MQ=255)
                                                                                                                     ctgctgGAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAgcc                                  <  1:5291571/150‑1 (MQ=255)
                                                                                                                         tgGAAGATCTTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATGCTGGTGCCTCTGAAGCGCGCCCAAGccgccg                              >  2:532529/1‑150 (MQ=255)
                                                                                                                          ggAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGa                             >  1:683135/1‑150 (MQ=255)
                                                                                                                           gAAGATATTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCACAACTTTATGGAGCCAAAGATCGCCGCAGATCTGATTAGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCACAAGCCGCAGat                            >  1:10633/1‑150 (MQ=255)
                                                                                                                            aaGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGata                           <  2:719209/150‑1 (MQ=255)
                                                                                                                             aGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGatat                          >  1:1731927/1‑150 (MQ=255)
                                                                                                                              gATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATAtt                         >  1:4666832/1‑150 (MQ=255)
                                                                                                                              gATATTCTCGAAACTCGCGATACCGCCAGCGGGATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCAGCTGAAGCGCGCCCAAGCCGCCGATAtt                         >  2:828742/1‑150 (MQ=255)
                                                                                                                                tatTCTCGAAACTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGAtcttct                       >  2:5215135/1‑150 (MQ=255)
                                                                                                                                 atTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTg                      >  2:3871844/1‑150 (MQ=255)
                                                                                                                                 atTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTg                      >  1:3612670/1‑150 (MQ=255)
                                                                                                                                 atTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTg                      >  2:3662306/1‑150 (MQ=255)
                                                                                                                                  ttCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTgg                     >  1:3061067/1‑150 (MQ=255)
                                                                                                                                        aaaCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCgttgt               >  1:5070745/1‑150 (MQ=255)
                                                                                                                                        aaaCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCgttgt               >  2:4821534/1‑150 (MQ=255)
                                                                                                                                        aaaCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCgttgt               <  2:295320/150‑1 (MQ=255)
                                                                                                                                         aaCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCgttgtt              <  2:1210305/150‑1 (MQ=255)
                                                                                                                                         aaCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCgttgtt              <  1:2429489/150‑1 (MQ=255)
                                                                                                                                          aCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTc             <  1:605773/150‑1 (MQ=255)
                                                                                                                                          aCTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTc             >  2:1169498/1‑150 (MQ=255)
                                                                                                                                           cTCGCGATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGAGGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCg            >  1:1648921/1‑150 (MQ=255)
                                                                                                                                               cgATACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGa        <  1:3184008/150‑1 (MQ=255)
                                                                                                                                                 aTACAGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAAc      >  1:2786722/1‑150 (MQ=255)
                                                                                                                                                     cGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCt  <  1:3278972/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGATCTGATTCGCGATGAGCATCCGCAAATTATCGCCACCATTCTGGTGCATCTGAAGCGCGCCCAAGCCGCCGATATTCTGGCGTTGTTCGATGAACGTCT  >  CP009273/2008520‑2008818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A