Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,093,906 C→A 22.7% E282* (GAG→TAG)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,093,9060CA22.7% 896.5 / 170.7 360E282* (GAG→TAG) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base C (139/138);  new base A (24/59);  total (163/197)
Fisher's exact test for biased strand distribution p-value = 6.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

AACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGA  >  CP009273/2093757‑2094055
                                                                                                                                                     |                                                                                                                                                     
aaCGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTc                                                                                                                                                       >  2:970504/1‑150 (MQ=255)
   gATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGt                                                                                                                                                    <  1:164560/150‑1 (MQ=255)
    aTTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACTCTGATCTTTCAGAGAAGAGAGATAACGTGCAAACACAGACTCGGTa                                                                                                                                                   >  2:5163754/1‑150 (MQ=255)
    aTTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTa                                                                                                                                                   >  2:1337838/1‑150 (MQ=255)
      tttGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAAt                                                                                                                                                 >  1:1448522/1‑150 (MQ=255)
      tttGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAAt                                                                                                                                                 <  1:4977988/150‑1 (MQ=255)
        tGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCa                                                                                                                                               <  2:1463391/150‑1 (MQ=255)
        tGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCa                                                                                                                                               <  1:3940318/150‑1 (MQ=255)
         gCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAg                                                                                                                                              >  2:5059461/1‑150 (MQ=255)
             aGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCACCACGCTGATCTTTCAGAGACGCGATATAACGTGCAAACACAGACTCGGTACTCAGCGAc                                                                                                                                          >  2:947809/1‑150 (MQ=255)
             aGATACAGCGCACGACGAACTTGTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGAc                                                                                                                                          <  2:683573/150‑1 (MQ=255)
              gATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACa                                                                                                                                         >  1:1484253/1‑150 (MQ=255)
                 aCAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCg                                                                                                                                      >  1:4264860/1‑150 (MQ=255)
                 aCAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCg                                                                                                                                      >  2:215473/1‑150 (MQ=255)
                   aGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGATATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGt                                                                                                                                    <  1:3476764/150‑1 (MQ=255)
                   aGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGATATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGt                                                                                                                                    <  1:3255735/150‑1 (MQ=255)
                   aGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGt                                                                                                                                    >  1:5176021/1‑150 (MQ=255)
                    gcgcACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGtt                                                                                                                                   <  2:2569224/150‑1 (MQ=255)
                     cgcACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGATAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTc                                                                                                                                  <  1:2073564/150‑1 (MQ=255)
                     cgcACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTc                                                                                                                                  <  2:5250580/150‑1 (MQ=255)
                     cgcACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTc                                                                                                                                  <  1:3000714/150‑1 (MQ=255)
                         cgacgaACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCg                                                                                                                              >  1:1870295/1‑150 (MQ=255)
                          gacgaACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCga                                                                                                                             >  2:2819176/1‑150 (MQ=255)
                             gaACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGAt                                                                                                                          <  1:4195486/150‑1 (MQ=255)
                              aaCTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATc                                                                                                                         <  2:2967208/150‑1 (MQ=255)
                               aCTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATcc                                                                                                                        >  2:3192938/1‑150 (MQ=255)
                                      cGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAgcgcgc                                                                                                                 >  2:4717591/1‑150 (MQ=255)
                                      cGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAgcgcgc                                                                                                                 <  1:259217/150‑1 (MQ=255)
                                        aTGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGctc                                                                                                               >  2:230754/1‑150 (MQ=255)
                                           aaCTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTctgg                                                                                                            >  2:634756/1‑150 (MQ=255)
                                            aCTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGACGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTctggc                                                                                                           >  2:2303029/1‑150 (MQ=255)
                                            aCTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTctggc                                                                                                           <  2:2507901/150‑1 (MQ=255)
                                              tCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTctggctg                                                                                                         >  1:2649602/1‑150 (MQ=255)
                                               cAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTctggctgg                                                                                                        >  1:2298406/1‑150 (MQ=255)
                                                aGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGt                                                                                                       >  1:4565258/1‑150 (MQ=255)
                                                  ccTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTcc                                                                                                     >  2:325251/1‑150 (MQ=255)
                                                   cTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCa                                                                                                    >  2:3645217/1‑150 (MQ=255)
                                                   cTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCa                                                                                                    <  2:1007975/150‑1 (MQ=255)
                                                      gTCGCCTTGTGGTTGTGCGTGAGTACAAGATAGAACTTTAGTTGCGGCAACACGCTTATATTTCAGAGAAGATATATAACGTGAAAAAACAGCATCGGTATTCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCtttt                                                                                                 <  1:4530741/150‑1 (MQ=255)
                                                      gTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCAttt                                                                                                 <  2:4232002/150‑1 (MQ=255)
                                                         gCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTAcc                                                                                              <  2:1050180/150‑1 (MQ=255)
                                                         gCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTAcc                                                                                              >  1:2451094/1‑150 (MQ=255)
                                                              ctggctgTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTAc                                                                                         >  1:3533154/1‑150 (MQ=255)
                                                                 gctgTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATATTTCAGAGAAGAGATATACCGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCtt                                                                                      <  1:4949193/150‑1 (MQ=255)
                                                                   tgtgCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTg                                                                                    <  2:3154351/150‑1 (MQ=255)
                                                                   tgtgCTTGCGGACCAGAGAGAACTTTAGATGAGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCTGTAATCAGCGACAGCGGTTCGCCGAGATCAAGAGCGCTCTGGCTGGTCCATTTACAGGTACCTTTg                                                                                    >  2:2721156/1‑150 (MQ=255)
                                                                     tgCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGtt                                                                                  <  1:3264734/150‑1 (MQ=255)
                                                                      gCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTa                                                                                 >  1:2949348/1‑150 (MQ=255)
                                                                      gCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTa                                                                                 <  1:48092/150‑1 (MQ=255)
                                                                        ttGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGc                                                                               >  2:5023975/1‑150 (MQ=255)
                                                                             gACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCtt                                                                          <  1:2525669/150‑1 (MQ=255)
                                                                             gACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCtt                                                                          <  1:2749519/150‑1 (MQ=255)
                                                                             gACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCtt                                                                          <  1:2199163/150‑1 (MQ=255)
                                                                             gACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTCCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCtt                                                                          <  1:1254816/150‑1 (MQ=255)
                                                                               ccAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCa                                                                        >  1:3828340/1‑150 (MQ=255)
                                                                                cAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCAt                                                                       >  2:4976471/1‑150 (MQ=255)
                                                                                 agagagAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTCCCTTTGTTAGCCGCTTCATc                                                                      >  2:4471986/1‑150 (MQ=255)
                                                                                 agagagAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATc                                                                      >  1:5069067/1‑150 (MQ=255)
                                                                                   agagaACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCa                                                                    >  2:1925697/1‑150 (MQ=255)
                                                                                    gagaACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTAACTTTGTTAGACGCTTCATACAg                                                                   >  1:1063045/1‑150 (MQ=255)
                                                                                     agaACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAgg                                                                  >  1:1220968/1‑150 (MQ=255)
                                                                                      gaACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGa                                                                 >  1:584845/1‑150 (MQ=255)
                                                                                        aCTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATc                                                               <  1:277720/150‑1 (MQ=255)
                                                                                           ttAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATACCGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATcaca                                                            <  1:1795927/150‑1 (MQ=255)
                                                                                           ttAGATGCGGCAACACGCTGAACTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGACCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATcaca                                                            >  2:2606543/1‑150 (MQ=255)
                                                                                            tAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACAt                                                           >  2:3463282/1‑150 (MQ=255)
                                                                                                   ggCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAg                                                    <  2:829398/150‑1 (MQ=255)
                                                                                                      aaCACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTa                                                 >  1:342183/1‑150 (MQ=255)
                                                                                                      aaCACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTa                                                 >  2:144192/1‑150 (MQ=255)
                                                                                                       acacGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAg                                                <  2:1734665/150‑1 (MQ=255)
                                                                                                         acGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGtt                                              <  2:4922479/150‑1 (MQ=255)
                                                                                                          cGCTGATCTTTCAGAGAAGAGATATAAAGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTa                                             >  2:1150868/1‑150 (MQ=255)
                                                                                                           gatGATCTTTCATAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTAc                                            <  1:4163171/148‑1 (MQ=255)
                                                                                                                tCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCt                                       <  1:1355526/150‑1 (MQ=255)
                                                                                                                 cTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCtt                                      >  1:2114863/1‑150 (MQ=255)
                                                                                                                  tttCAGAGAAGAGATATAACGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTc                                     <  2:3854612/150‑1 (MQ=255)
                                                                                                                    tCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCAt                                   <  1:3735889/150‑1 (MQ=255)
                                                                                                                     cAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATc                                  >  2:4534487/1‑150 (MQ=255)
                                                                                                                      agagaagagaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCt                                 >  1:1149367/1‑150 (MQ=255)
                                                                                                                       gagaagagaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCtt                                <  1:3961436/150‑1 (MQ=255)
                                                                                                                        agaagagaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCttt                               <  1:525632/150‑1 (MQ=255)
                                                                                                                          aagagaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCttttt                             >  1:412075/1‑150 (MQ=255)
                                                                                                                           agagaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCtttttt                            >  2:5293173/1‑150 (MQ=255)
                                                                                                                            gagaAATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGATCGCCGAGATCCAGCGCGCTATCTCTGGACCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTATTg                           >  1:4850311/1‑150 (MQ=255)
                                                                                                                             agaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTgg                          <  1:3103919/150‑1 (MQ=255)
                                                                                                                              gaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGt                         >  2:3531271/1‑150 (MQ=255)
                                                                                                                              gaTATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGt                         <  1:842655/150‑1 (MQ=255)
                                                                                                                                  taACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGATACCGTCTTCATCTTTTTTGTTGAAg                     >  1:4682981/1‑150 (MQ=255)
                                                                                                                                   aaCGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTGTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGa                    >  2:4087385/1‑150 (MQ=255)
                                                                                                                                    aCGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGat                   <  2:3348567/150‑1 (MQ=255)
                                                                                                                                    aCGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGGACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGat                   <  2:926961/150‑1 (MQ=255)
                                                                                                                                    aCGTGCAAACACAGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTAACGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGat                   <  1:743912/150‑1 (MQ=255)
                                                                                                                                      gTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGatat                 <  1:4769063/150‑1 (MQ=255)
                                                                                                                                       tGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATc                <  2:2207537/150‑1 (MQ=255)
                                                                                                                                        gCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCt               <  2:1852015/150‑1 (MQ=255)
                                                                                                                                          aaaCACTGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTAACTTTGTGAGCCGATTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCttt             <  1:4361799/150‑1 (MQ=255)
                                                                                                                                           aaCACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTg            >  1:366418/1‑150 (MQ=255)
                                                                                                                                              acaGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTg         <  2:619458/150‑1 (MQ=255)
                                                                                                                                                aGACTAGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGAt       <  1:3201192/150‑1 (MQ=255)
                                                                                                                                                   cTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTc    >  2:3710056/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTTAGATGCGGCAACACGCTGATCTTTCAGAGAAGAGATATAACGTGCAAACACAGACTCGGTAATCAGCGACAGCGGTTCGCCGAGATCCAGCGCGCTCTGGCTGGTCCATTTACCGGTACCTTTGTTAGCCGCTTCATCCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGA  >  CP009273/2093757‑2094055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A