Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,226,594 G→T 25.8% G80C (GGT→TGT)  sanA → vancomycin high temperature exclusion protein, DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,226,5940GT25.8% 809.5 / 213.3 369G80C (GGT→TGT) sanAvancomycin high temperature exclusion protein, DUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (132/140);  new base T (49/47);  total (182/187)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

AAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGATTCCATC  >  CP009273/2226445‑2226743
                                                                                                                                                     |                                                                                                                                                     
aaaCCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCg                                                                                                                                                       >  2:2747426/1‑150 (MQ=255)
aaaCCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCg                                                                                                                                                       <  2:1884563/150‑1 (MQ=255)
  aCCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAACTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCggt                                                                                                                                                     <  2:5043041/150‑1 (MQ=255)
    cgcgCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCggtaa                                                                                                                                                   <  2:2781149/150‑1 (MQ=255)
     gcgcCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCggtaag                                                                                                                                                  <  2:2942986/150‑1 (MQ=255)
       gcCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCggtaaggt                                                                                                                                                >  1:1090421/1‑150 (MQ=255)
        ccTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCtgtaaggta                                                                                                                                               <  2:1233628/150‑1 (MQ=255)
         cTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCggtaaggtaa                                                                                                                                              <  2:67310/150‑1 (MQ=255)
          ttATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTaaa                                                                                                                                             <  1:5203210/150‑1 (MQ=255)
          ttATATCTACGACGAATTGCAGGATCTCCCCTAACGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTaaa                                                                                                                                             <  1:3040459/150‑1 (MQ=255)
            atatCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAAtt                                                                                                                                           >  2:3360122/1‑150 (MQ=255)
               tctcCGACGTATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAATTATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATc                                                                                                                                        <  1:2727024/150‑1 (MQ=255)
               tCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATc                                                                                                                                        >  1:619495/1‑150 (MQ=255)
                cTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCt                                                                                                                                       <  2:1127252/150‑1 (MQ=255)
                cTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCt                                                                                                                                       >  1:2905221/1‑150 (MQ=255)
                  acgacgAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTAt                                                                                                                                     <  2:560296/150‑1 (MQ=255)
                     acgaATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTAc                                                                                                                                  >  1:1618585/1‑150 (MQ=255)
                     acgaATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTAc                                                                                                                                  <  2:247876/150‑1 (MQ=255)
                     acgaATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTAc                                                                                                                                  >  2:430695/1‑150 (MQ=255)
                      cgaATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACt                                                                                                                                 <  1:2259824/150‑1 (MQ=255)
                       gaATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTCTTACTg                                                                                                                                >  2:3583450/1‑150 (MQ=255)
                          ttGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAgc                                                                                                                             <  2:3990616/150‑1 (MQ=255)
                           tGCAGGATCTCCCCTACCGCCAGGTCGGGGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAgcg                                                                                                                            >  1:1733816/1‑150 (MQ=255)
                              aGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAgcggcg                                                                                                                         <  2:3685836/150‑1 (MQ=255)
                              aGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAgcggcg                                                                                                                         >  1:3124339/1‑150 (MQ=255)
                              aGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAgcggcg                                                                                                                         <  1:3692122/150‑1 (MQ=255)
                                  tctcCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATaa                                                                                                                     <  2:4907090/150‑1 (MQ=255)
                                   ctcCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAAc                                                                                                                    >  1:4675045/1‑150 (MQ=255)
                                      cccTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGAGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCa                                                                                                                 <  1:4238747/150‑1 (MQ=255)
                                         tACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTg                                                                                                              <  1:4058416/150‑1 (MQ=255)
                                         tACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTg                                                                                                              <  1:5289537/150‑1 (MQ=255)
                                              ccAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAg                                                                                                         <  2:5179170/150‑1 (MQ=255)
                                              ccAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAg                                                                                                         >  2:3221499/1‑150 (MQ=255)
                                                aGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGtt                                                                                                       <  1:2822399/150‑1 (MQ=255)
                                                  gTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTtat                                                                                                     <  2:4097587/150‑1 (MQ=255)
                                                   tCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTtata                                                                                                    <  1:1919351/150‑1 (MQ=255)
                                                        gtggtgCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAg                                                                                               <  1:3756078/150‑1 (MQ=255)
                                                        gtggtgCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGCAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAg                                                                                               <  1:1991381/150‑1 (MQ=255)
                                                           gtgCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCg                                                                                            >  1:4765784/1‑150 (MQ=255)
                                                            tgCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGa                                                                                           >  2:4229252/1‑150 (MQ=255)
                                                             gCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGAt                                                                                          <  1:4849149/150‑1 (MQ=255)
                                                              cTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATg                                                                                         >  1:2594503/1‑150 (MQ=255)
                                                               tCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGa                                                                                        >  1:2528743/1‑150 (MQ=255)
                                                                  gAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCa                                                                                     >  1:3996711/1‑150 (MQ=255)
                                                                   aaCAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCAt                                                                                    <  2:1937214/150‑1 (MQ=255)
                                                                   aaCAGCAAAATATTATCGTACGGGCGTAATTACTCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCAt                                                                                    <  1:4607070/150‑1 (MQ=255)
                                                                    aCAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATg                                                                                   <  2:3529619/150‑1 (MQ=255)
                                                                      aGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATgcg                                                                                 >  2:3143508/1‑150 (MQ=255)
                                                                      aGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATgcg                                                                                 <  1:3201688/150‑1 (MQ=255)
                                                                      aGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATgcg                                                                                 >  2:2001745/1‑150 (MQ=255)
                                                                         aaaaTATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCaa                                                                              >  2:2663509/1‑150 (MQ=255)
                                                                         aaaaTATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCaa                                                                              >  1:2932732/1‑150 (MQ=255)
                                                                             tattatCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGAt                                                                          >  2:926440/1‑150 (MQ=255)
                                                                             tattatCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGAt                                                                          <  2:1699959/150‑1 (MQ=255)
                                                                              attatCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGAtt                                                                         >  2:4376509/1‑150 (MQ=255)
                                                                                   cGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATc                                                                    >  2:576173/1‑150 (MQ=255)
                                                                                   cGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATc                                                                    <  1:3640136/150‑1 (MQ=255)
                                                                                    gTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCTATGACCATGCGCAAAGATTTAAtag                                                                   >  1:1362400/1‑148 (MQ=255)
                                                                                    gTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCg                                                                   >  2:3818753/1‑150 (MQ=255)
                                                                                     tACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCgc                                                                  >  1:4831221/1‑150 (MQ=255)
                                                                                     tACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCgc                                                                  >  1:1671322/1‑150 (MQ=255)
                                                                                         ggCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCgctgct                                                              >  2:4943507/1‑150 (MQ=255)
                                                                                         ggCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCgctgct                                                              <  2:4792033/150‑1 (MQ=255)
                                                                                             taattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtg                                                          >  1:1521319/1‑150 (MQ=255)
                                                                                              aattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtgt                                                         >  1:210332/1‑150 (MQ=255)
                                                                                              aattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtgt                                                         >  1:812030/1‑150 (MQ=255)
                                                                                              aattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtgt                                                         >  1:3360310/1‑150 (MQ=255)
                                                                                              aattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAACAAAAAAAGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtgt                                                         >  2:446884/1‑150 (MQ=255)
                                                                                              aattaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCATATAACAGCGGTAAGGTTAATTATATATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGgtgt                                                         <  1:4976234/150‑1 (MQ=255)
                                                                                               attaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTc                                                        <  1:3242561/150‑1 (MQ=255)
                                                                                               attaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTc                                                        >  2:3264964/1‑150 (MQ=255)
                                                                                               attaatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTc                                                        >  1:4215767/1‑150 (MQ=255)
                                                                                                  aatCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGAc                                                     >  1:242413/1‑150 (MQ=255)
                                                                                                      aGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCAt                                                 >  1:2665828/1‑150 (MQ=255)
                                                                                                      aGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCAt                                                 >  1:4532958/1‑150 (MQ=255)
                                                                                                      aGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTCAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCAt                                                 >  2:873613/1‑150 (MQ=255)
                                                                                                       gTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCagc                                                >  1:3070257/1‑148 (MQ=255)
                                                                                                        tattatCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCTACCCATCa                                               >  1:5115369/1‑150 (MQ=255)
                                                                                                        tattatCGCTACCGCATTCAAGGAGCAATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCa                                               >  2:2272726/1‑150 (MQ=255)
                                                                                                         attatCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAg                                              >  2:5249876/1‑150 (MQ=255)
                                                                                                         attatCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAg                                              >  1:445208/1‑150 (MQ=255)
                                                                                                               gCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTg                                        <  2:2306879/150‑1 (MQ=255)
                                                                                                                    cGCATTCAAGGAGCGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTtctc                                   >  1:1050097/1‑150 (MQ=255)
                                                                                                                       aTTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGAt                                >  1:4092736/1‑150 (MQ=255)
                                                                                                                       aTTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGAt                                >  2:2149547/1‑150 (MQ=255)
                                                                                                                        ttCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGAtt                               <  2:2381698/150‑1 (MQ=255)
                                                                                                                         tCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTa                              <  2:4826204/150‑1 (MQ=255)
                                                                                                                               aGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAgg                        >  2:4577400/1‑150 (MQ=255)
                                                                                                                                 cGATTAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCt                      >  1:3886309/1‑150 (MQ=255)
                                                                                                                                    ttAATGACTATAACAGAGTTAAGGTAAATTATCTATTACTGAGCGTCGATAACGCATTGCAAATTTATAATGAGCCGATGAACATTCGCAAAGTTTTAATCGCTGATGGTGTCGACCCTTCAGATATTGTTATCGTTTTCGCAGGCTTTc                   <  2:2564980/150‑1 (MQ=255)
                                                                                                                                     tAATGCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCg                  <  2:821576/150‑1 (MQ=255)
                                                                                                                                         gCCTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACg              >  2:5097802/1‑150 (MQ=255)
                                                                                                                                          ccTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGc             <  2:4699635/150‑1 (MQ=255)
                                                                                                                                           cTATAACAGCTGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCt            >  1:1195137/1‑150 (MQ=255)
                                                                                                                                           cTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCt            >  2:1620028/1‑150 (MQ=255)
                                                                                                                                            tataACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTg           <  1:883473/150‑1 (MQ=255)
                                                                                                                                            tataACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTg           <  2:1347863/150‑1 (MQ=255)
                                                                                                                                               aaCAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGAt        <  2:1431365/150‑1 (MQ=255)
                                                                                                                                                aCAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGAtt       <  2:484552/150‑1 (MQ=255)
                                                                                                                                                    cGGTAAGGTAAATTATCTATTTCTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGATTCCAt   <  1:2149831/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCGCGCCTTATATCTACGACGAATTGCAGGATCTCCCCTACCGCCAGGTCGGTGTGGTGCTCGGAACAGCAAAATATTATCGTACTGGCGTAATTAATCAGTATTATCGCTACCGCATTCAAGGAGCGATTAATGCCTATAACAGCGGTAAGGTAAATTATCTATTACTGAGCGGCGATAACGCATTGCAAAGTTATAATGAGCCGATGACCATGCGCAAAGATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGATTCCATC  >  CP009273/2226445‑2226743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A