Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,400,876 C→T 17.0% intergenic (‑756/‑164) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,400,8760CT17.0% 900.2 / 139.5 336intergenic (‑756/‑164)lrhA/alaAtranscriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
Reads supporting (aligned to +/- strand):  ref base C (134/144);  new base T (19/38);  total (154/182)
Fisher's exact test for biased strand distribution p-value = 4.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

CGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAA  >  CP009273/2400728‑2401025
                                                                                                                                                    |                                                                                                                                                     
cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCt                                                                                                                                                      >  1:101625/1‑150 (MQ=255)
cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCt                                                                                                                                                      <  2:2289408/150‑1 (MQ=255)
 gTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTc                                                                                                                                                     >  2:4135996/1‑150 (MQ=255)
 gTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATACACTATATAACATAGAATGTAAAGCATATACACCTc                                                                                                                                                     >  1:3605530/1‑150 (MQ=255)
    aTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCatt                                                                                                                                                  <  1:4623588/150‑1 (MQ=255)
      gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCattat                                                                                                                                                <  2:3907383/150‑1 (MQ=255)
      gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCattat                                                                                                                                                <  2:3969379/150‑1 (MQ=255)
      gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCattat                                                                                                                                                >  1:5055292/1‑150 (MQ=255)
        ggAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                                              >  2:4394829/1‑150 (MQ=255)
        ggAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                                              <  2:2748753/150‑1 (MQ=255)
            atatCATCATCGCGTTTCATTAGAGAATTCATAAATGAGGCATTTTCACAACATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTc                                                                                                                                          <  2:806992/150‑1 (MQ=255)
             tatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCa                                                                                                                                         >  1:4470975/1‑150 (MQ=255)
              atcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCat                                                                                                                                        >  1:2593074/1‑150 (MQ=255)
              atcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCat                                                                                                                                        >  1:1188926/1‑150 (MQ=255)
               tcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCatt                                                                                                                                       <  1:1223160/150‑1 (MQ=255)
               tcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCatt                                                                                                                                       >  2:4782980/1‑150 (MQ=255)
                catcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCatta                                                                                                                                      >  1:4946950/1‑150 (MQ=255)
                 atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACCGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCattat                                                                                                                                     <  1:1311317/150‑1 (MQ=255)
                 atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCattat                                                                                                                                     <  2:4687964/150‑1 (MQ=255)
                 atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                                                     <  1:4528128/150‑1 (MQ=255)
                  tcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCattatt                                                                                                                                    <  2:4249550/150‑1 (MQ=255)
                  tcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattatt                                                                                                                                    <  1:4585942/150‑1 (MQ=255)
                   catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAATAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCAttatta                                                                                                                                   <  1:1973967/150‑1 (MQ=255)
                      cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGt                                                                                                                                <  2:2783844/150‑1 (MQ=255)
                         gTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttat                                                                                                                             <  2:1424141/150‑1 (MQ=255)
                            tCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTaa                                                                                                                          <  1:1677150/150‑1 (MQ=255)
                             cATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAc                                                                                                                         >  2:1719537/1‑150 (MQ=255)
                             cATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAc                                                                                                                         >  1:781256/1‑150 (MQ=255)
                              aTTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAATACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACa                                                                                                                        >  2:1014110/1‑150 (MQ=255)
                              aTTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACa                                                                                                                        <  2:2260328/150‑1 (MQ=255)
                               ttAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCATTATTAAGTTATTAACAg                                                                                                                       >  1:4307996/1‑150 (MQ=255)
                                  gTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcac                                                                                                                    >  1:4193398/1‑150 (MQ=255)
                                   tGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                                                   >  1:1424567/1‑150 (MQ=255)
                                     aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATCATTTTGTCATTATTAAGTTATTAACAGCACAAt                                                                                                                 <  2:1911431/150‑1 (MQ=255)
                                      aTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATc                                                                                                                <  1:2159740/150‑1 (MQ=255)
                                          ttAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACTCCTCATTATTTTGTCATTATTAAGTGATTAACAGCACAATCGAGc                                                                                                            >  2:4066083/1‑150 (MQ=255)
                                          ttAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGc                                                                                                            >  2:1300845/1‑150 (MQ=255)
                                          ttAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGc                                                                                                            >  2:799575/1‑150 (MQ=255)
                                            aaaTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAGCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                                          >  2:3485300/1‑150 (MQ=255)
                                                gAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTccc                                                                                                      >  2:5088327/1‑150 (MQ=255)
                                                     aTTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTg                                                                                                 >  2:2493186/1‑150 (MQ=255)
                                                     aTTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTg                                                                                                 >  2:17967/1‑150 (MQ=255)
                                                     aTTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTg                                                                                                 >  2:705101/1‑150 (MQ=255)
                                                      ttttCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTgg                                                                                                <  1:1532188/150‑1 (MQ=255)
                                                            cacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAt                                                                                          <  1:2203529/150‑1 (MQ=255)
                                                                 ttATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAt                                                                                     <  2:3332662/150‑1 (MQ=255)
                                                                 ttATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAt                                                                                     <  1:3067718/150‑1 (MQ=255)
                                                                  tATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAtt                                                                                    <  2:150308/150‑1 (MQ=255)
                                                                  tATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAtt                                                                                    >  2:1533193/1‑150 (MQ=255)
                                                                    tCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCt                                                                                  <  1:227846/150‑1 (MQ=255)
                                                                     cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTg                                                                                 <  2:3623004/150‑1 (MQ=255)
                                                                     cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTg                                                                                 >  1:2613626/1‑150 (MQ=255)
                                                                       tACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCa                                                                               <  2:2907088/150‑1 (MQ=255)
                                                                       tACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCa                                                                               <  1:569273/150‑1 (MQ=255)
                                                                         cAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGa                                                                             >  1:5245571/1‑150 (MQ=255)
                                                                          aGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAc                                                                            >  2:3535326/1‑150 (MQ=255)
                                                                          aGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAc                                                                            >  2:3151532/1‑150 (MQ=255)
                                                                            aTAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                                          >  1:4082409/1‑150 (MQ=255)
                                                                             tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                                         <  1:4394829/150‑1 (MQ=255)
                                                                                aaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaa                                                                      >  2:4426976/1‑150 (MQ=255)
                                                                                aaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaa                                                                      >  2:938739/1‑150 (MQ=255)
                                                                                aaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaa                                                                      >  1:2493504/1‑150 (MQ=255)
                                                                                       aGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCg                                                               >  2:2130093/1‑150 (MQ=255)
                                                                                         aCTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                                             <  2:2820066/150‑1 (MQ=255)
                                                                                           ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCt                                                           >  2:1955280/1‑150 (MQ=255)
                                                                                           ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCt                                                           <  2:1120703/150‑1 (MQ=255)
                                                                                            tACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTg                                                          <  1:2958440/150‑1 (MQ=255)
                                                                                              cAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTggg                                                        >  2:4279816/1‑150 (MQ=255)
                                                                                               aaatAAGAATCAGAACAATTCACTATATAACAGATAATGTAAATCATATACACCTCATTATTTTGTCATTATTAAGATATTAACAGCAAAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTgggg                                                       >  2:584645/4‑150 (MQ=255)
                                                                                               aaTTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCAGCAGACCTTCAAAACACCGTCCTgggg                                                       >  1:2106198/1‑150 (MQ=255)
                                                                                               aaTTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTgggg                                                       >  2:864183/1‑150 (MQ=255)
                                                                                                 ttAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGa                                                     >  1:3643805/1‑150 (MQ=255)
                                                                                                  tAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAg                                                    <  2:4639244/150‑1 (MQ=255)
                                                                                                    aGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTa                                                  <  2:514470/150‑1 (MQ=255)
                                                                                                      aaTCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                                                >  2:376653/1‑150 (MQ=255)
                                                                                                        tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACAtt                                              <  2:1158339/150‑1 (MQ=255)
                                                                                                          aGAACAATTCACTATATAACATTGAATGTAAAGCGTATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGt                                            >  2:1201412/1‑150 (MQ=255)
                                                                                                          aGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGt                                            >  1:1278961/1‑150 (MQ=255)
                                                                                                           gAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGtt                                           <  1:801197/150‑1 (MQ=255)
                                                                                                            aaCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTCTTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTc                                          <  1:908699/150‑1 (MQ=255)
                                                                                                            aaCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTc                                          >  1:2813236/1‑150 (MQ=255)
                                                                                                              cAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTa                                        <  1:3105205/150‑1 (MQ=255)
                                                                                                               aaTTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTaa                                       >  2:1604732/1‑150 (MQ=255)
                                                                                                                  tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt                                    <  2:4519268/150‑1 (MQ=255)
                                                                                                                       atataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCTTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACtt                               >  2:2674256/1‑150 (MQ=255)
                                                                                                                        tataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTc                              <  1:3903506/150‑1 (MQ=255)
                                                                                                                         ataACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTcc                             >  2:5254021/1‑150 (MQ=255)
                                                                                                                           aaCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAc                           >  2:485636/1‑150 (MQ=255)
                                                                                                                           aaCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAc                           >  1:2329721/1‑150 (MQ=255)
                                                                                                                               ttGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCa                       <  1:3180102/150‑1 (MQ=255)
                                                                                                                               ttGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCa                       <  1:4138773/150‑1 (MQ=255)
                                                                                                                                tgaaTGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAg                      >  1:4823049/4‑150 (MQ=255)
                                                                                                                                        aaGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGc              <  1:705101/150‑1 (MQ=255)
                                                                                                                                            atatACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATa          >  1:3958913/1‑150 (MQ=255)
                                                                                                                                               taCACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACa       >  2:1236972/1‑150 (MQ=255)
                                                                                                                                                acacTTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAg      <  2:2077772/150‑1 (MQ=255)
                                                                                                                                                acacCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAg      <  1:3234791/150‑1 (MQ=255)
                                                                                                                                                 cacCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGc     <  1:33777/150‑1 (MQ=255)
                                                                                                                                                 cacCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGc     <  2:307341/150‑1 (MQ=255)
                                                                                                                                                   ccTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCaa   <  2:2593074/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAA  >  CP009273/2400728‑2401025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A