Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,448 A→C 32.1% intergenic (‑206/+53) cvpA ← / ← dedD colicin V production membrane protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,4480AC32.1% 311.3 / inf 374intergenic (‑206/+53)cvpA/dedDcolicin V production membrane protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (127/127);  new base C (62/57);  total (189/185)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

Only 100 of 378 total aligned reads displayed.

AAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCATCCG  >  CP009273/2424299‑2424595
                                                                                                                                                     |                                                                                                                                                   
 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                                    <  1:2259573/150‑1 (MQ=255)
 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                                    >  1:4775711/1‑150 (MQ=255)
  gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcc                                                                                                                                                   >  2:1115593/1‑150 (MQ=255)
     aaCGTTTGCGTAGGGATTTCCGTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCt                                                                                                                                                <  2:2144472/150‑1 (MQ=255)
      aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                                               <  1:2426841/150‑1 (MQ=255)
        gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCg                                                                                                                                             >  1:5122732/1‑150 (MQ=255)
        gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCg                                                                                                                                             >  1:4869950/1‑150 (MQ=255)
          ttGTGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTa                                                                                                                                           <  2:4229034/150‑1 (MQ=255)
            gCGGAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAgg                                                                                                                                         <  2:4953085/150‑1 (MQ=255)
             cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                                                                                                                        >  2:4917346/1‑150 (MQ=255)
             cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                                                                                                                        >  2:2608427/1‑150 (MQ=255)
              gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGcc                                                                                                                                       >  2:1599904/1‑150 (MQ=255)
              gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGAAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGcc                                                                                                                                       >  1:654198/1‑150 (MQ=255)
              gTAGGGATTTCCTACCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGACACGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCGGCGTCGGcc                                                                                                                                       >  1:528423/1‑150 (MQ=39)
               tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCt                                                                                                                                      <  2:611139/150‑1 (MQ=255)
               tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                                                                                                                      >  1:3807413/1‑150 (MQ=255)
               tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                                                                                                                      <  2:4640927/150‑1 (MQ=255)
                   gATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                                                                                                                  >  2:3663786/1‑150 (MQ=255)
                    aTTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATaa                                                                                                                                 >  1:1660084/1‑150 (MQ=255)
                       tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGAc                                                                                                                              <  2:4075524/150‑1 (MQ=255)
                        ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGAcg                                                                                                                             <  2:4769725/150‑1 (MQ=255)
                                 gcATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCgc                                                                                                                    <  1:1553717/150‑2 (MQ=255)
                                 gcATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGt                                                                                                                    >  1:3476004/1‑150 (MQ=255)
                                       aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                                                                                                              >  1:2536687/1‑150 (MQ=255)
                                       aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                                                                                                              >  1:2862393/1‑150 (MQ=255)
                                       aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                                                                                                              <  1:3023/150‑1 (MQ=255)
                                        tAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa                                                                                                             <  2:52858/150‑1 (MQ=255)
                                         aaaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg                                                                                                            >  2:3777990/1‑150 (MQ=255)
                                         aaaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCATTGCGGCATCGGGCAATGCGAGTCGGATGCGGCGAAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg                                                                                                            >  1:2635994/1‑150 (MQ=255)
                                          aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                                                                                           >  1:125786/1‑150 (MQ=255)
                                           aaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                          >  1:4747210/1‑150 (MQ=255)
                                           aaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                          >  1:3394318/1‑150 (MQ=255)
                                            caTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                                         <  2:5047544/149‑1 (MQ=255)
                                            aaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                                         <  1:2927995/150‑1 (MQ=255)
                                               ggCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATACGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                                                                                      >  2:1840194/1‑150 (MQ=255)
                                                   gtGAAAAAATATTCAACGCCATCGCCTTTTTAGGCCTTGGCGGCATCGGGCATGGCGTGTCGGTTGCGGCGTAAACGCCTTAACCGACCTACGGTTCTCCCCCTGCGTCGGCCTGTTAAGACGCGCCAGCGTCGCATCAGGCAAGACCGt                                                                                                  <  2:2412565/149‑1 (MQ=37)
                                                      aaaaaaTATTCCACGCCATCGACTTTTTTTGCGTTTGCGTCATCGGGCTTTGCGTGTCGTATGCGGCTTAACCGCCTTATCCGACCTACGGTTCTCCCCCTGCTTTTGACTTATATGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                                               <  2:1984482/150‑1 (MQ=255)
                                                      aaaaaaTATACAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAACCGCCTTATCCGACCTACGGTTCTACCCCTGCGTTGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                                               <  2:2360529/150‑1 (MQ=255)
                                                       aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGGAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATCAGACGCGCCAGCGTCGCATCAGGCACGACCggagta                                                                                              >  2:3293952/1‑145 (MQ=255)
                                                        aaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTaa                                                                                             >  2:2245904/1‑150 (MQ=255)
                                                           atatTCTACGCCTTCTACTTTTTATGCCTTTGCGGCAGCGGGCAATGTGTGTCGGTTGCGGCGTAAACGCCTTATCGGTCCTTCGGTTCTCCCCGTGGGTTGGCCTGTTATGAGGCGCCATCGTGGCATCTGGCAAGACCGTATTAATTc                                                                                          <  1:4338458/150‑1 (MQ=255)
                                                           atatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTc                                                                                          <  1:3433088/150‑1 (MQ=255)
                                                            tatTCAACGCCATCTACTTTTTATGCCTTTGCGGAATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATACGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                                                         <  2:2339830/150‑1 (MQ=255)
                                                             atTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCgg                                                                                        >  1:4592349/1‑150 (MQ=255)
                                                              ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGc                                                                                       >  2:4738062/1‑150 (MQ=255)
                                                                    gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAg                                                                                 <  1:205043/150‑1 (MQ=255)
                                                                          gACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCAtt                                                                           <  2:4289232/150‑1 (MQ=255)
                                                                            cTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAc                                                                         >  2:2304122/1‑150 (MQ=255)
                                                                                ttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACg                                                                     <  1:5230189/150‑1 (MQ=255)
                                                                                   ggCCTTTGCGGCTTCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTCGGCCTGATACGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa                                                                  <  1:4672578/149‑1 (MQ=255)
                                                                                    gCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCAc                                                                 <  2:674025/150‑1 (MQ=255)
                                                                                       tttGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                                              >  1:1914034/1‑150 (MQ=255)
                                                                                           cGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGcc                                                          <  1:874317/150‑1 (MQ=255)
                                                                                            ggCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCa                                                         <  2:4809284/150‑1 (MQ=255)
                                                                                               ttggggCATTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCTTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGaa                                                      <  1:4073773/147‑1 (MQ=255)
                                                                                               aTCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGaa                                                      >  2:3914740/1‑150 (MQ=255)
                                                                                               aTCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGaa                                                      >  1:770745/1‑150 (MQ=255)
                                                                                               aTCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGaa                                                      >  1:1317476/1‑150 (MQ=255)
                                                                                                  gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGt                                                   >  1:705740/1‑150 (MQ=255)
                                                                                                   ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCCGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGtt                                                  <  2:5037773/150‑1 (MQ=255)
                                                                                                   ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGtt                                                  <  2:156438/150‑1 (MQ=255)
                                                                                                     cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                                                >  2:1468666/1‑150 (MQ=255)
                                                                                                     cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                                                <  2:1422453/150‑1 (MQ=255)
                                                                                                     cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                                                >  2:1436058/1‑150 (MQ=255)
                                                                                                       ttGCGTGTCGGTTGCGGCTTAAACGCCTTATCCGACCTACGTTTCTCCCCCTGCGTTGGCCTTATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCtt                                              <  2:136377/149‑1 (MQ=38)
                                                                                                       ttGCGTGTCGGATGCGGCTTAAACGCCTTATCCGACCTAGGGTTCTACCCCGGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCtt                                              <  1:5022957/149‑1 (MQ=255)
                                                                                                       aggCGTGTCGGTTGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCtt                                              <  2:2442671/148‑1 (MQ=255)
                                                                                                       aTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCtt                                              >  2:4440584/1‑150 (MQ=255)
                                                                                                        tGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTc                                             >  2:2531024/1‑150 (MQ=255)
                                                                                                          cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCaa                                           >  1:5058905/1‑150 (MQ=255)
                                                                                                           gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAAc                                          >  1:1947175/1‑150 (MQ=255)
                                                                                                            tgtCGGTTGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                                         <  1:4553999/150‑1 (MQ=255)
                                                                                                            tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                                         >  2:1249174/1‑150 (MQ=255)
                                                                                                            tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                                         <  1:4086081/150‑1 (MQ=255)
                                                                                                            tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                                         >  2:4182039/1‑150 (MQ=255)
                                                                                                             gtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTc                                        >  2:2724553/1‑150 (MQ=255)
                                                                                                                 gATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCAccc                                    >  2:1749432/1‑150 (MQ=255)
                                                                                                                          tAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTTGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCt                           <  1:39793/150‑1 (MQ=255)
                                                                                                                          tAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGTCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCt                           >  2:19062/1‑150 (MQ=255)
                                                                                                                          tAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCt                           >  1:998980/1‑150 (MQ=255)
                                                                                                                           aaaCGCCTTATCCGACCTACGTTTCTACCCCTGCGTAGGCATGATAAGACGCGCCCGGGTCGCTACAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCtt                          <  1:2437767/150‑1 (MQ=255)
                                                                                                                            aaCGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCttt                         <  2:3434768/150‑1 (MQ=255)
                                                                                                                              cGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCa                       >  1:3627926/1‑150 (MQ=255)
                                                                                                                               gCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAg                      >  2:3050243/1‑150 (MQ=255)
                                                                                                                                ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGc                     <  2:4834868/150‑1 (MQ=255)
                                                                                                                                      ccGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCt               <  2:2833048/150‑1 (MQ=255)
                                                                                                                                      ccGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCt               <  2:889326/150‑1 (MQ=255)
                                                                                                                                      ccGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCt               <  2:2047642/150‑1 (MQ=255)
                                                                                                                                       cGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCtt              <  2:3112576/150‑1 (MQ=255)
                                                                                                                                       cGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCtt              <  1:1044472/150‑1 (MQ=255)
                                                                                                                                        gACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCttt             >  1:1156961/1‑150 (MQ=255)
                                                                                                                                         aCCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAACAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTc            <  1:1115593/150‑1 (MQ=255)
                                                                                                                                          ccTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCg           >  2:293754/1‑150 (MQ=255)
                                                                                                                                           cTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGa          >  1:1812550/1‑150 (MQ=255)
                                                                                                                                             aCGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAgg        <  2:1660084/150‑1 (MQ=255)
                                                                                                                                               ggTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCa      >  2:337760/1‑150 (MQ=255)
                                                                                                                                               ggTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCa      >  1:1407505/1‑150 (MQ=255)
                                                                                                                                                gTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCAt     >  1:3382140/1‑150 (MQ=255)
                                                                                                                                                 ttCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCATc    >  2:575170/1‑150 (MQ=255)
                                                                                                                                                  tctcCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCATcc   <  2:5157104/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCATCCG  >  CP009273/2424299‑2424595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A