Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,512,031 C→A 13.1% S29* (TCA→TAA)  yfeC → DUF1323 family putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,512,0310CA13.1% 961.4 / 75.6 300S29* (TCA→TAA) yfeCDUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (128/132);  new base A (19/21);  total (147/153)
Fisher's exact test for biased strand distribution p-value = 8.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TGTTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTG  >  CP009273/2511882‑2512179
                                                                                                                                                     |                                                                                                                                                    
tgtTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATGAATAAATGGGTACGCAAGGAAGGCTGGACAACGTc                                                                                                                                                      >  2:1103282/1‑150 (MQ=255)
 gtTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCa                                                                                                                                                     >  2:4819463/1‑150 (MQ=255)
    ataAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCa                                                                                                                                                  >  2:3690210/1‑150 (MQ=255)
     taAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCaa                                                                                                                                                 <  2:4080792/150‑1 (MQ=255)
       aaaTGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCaaaa                                                                                                                                               >  2:29130/1‑150 (MQ=255)
       aaaTGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCaaaa                                                                                                                                               >  1:2711225/1‑150 (MQ=255)
       aaaTGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCaaaa                                                                                                                                               >  1:5019776/1‑150 (MQ=255)
         aTGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAAcc                                                                                                                                             >  2:3354049/1‑150 (MQ=255)
          tGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCt                                                                                                                                            >  2:1255934/1‑150 (MQ=255)
          tGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCt                                                                                                                                            >  2:2265017/1‑150 (MQ=255)
           gAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTg                                                                                                                                           <  2:4551619/150‑1 (MQ=255)
           gAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTg                                                                                                                                           >  2:2712603/1‑150 (MQ=255)
             actactAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGc                                                                                                                                         <  2:2619661/150‑1 (MQ=255)
               tactaATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGt                                                                                                                                       >  1:5064026/1‑150 (MQ=255)
                  taATATACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATTGGTACGCAAGGAAGTCTGGACAACGTCACCAAAACATTGCGTCCa                                                                                                                                    >  1:3469967/1‑150 (MQ=255)
                  taATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCa                                                                                                                                    <  2:2191899/150‑1 (MQ=255)
                     tAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAggg                                                                                                                                 >  1:382294/1‑150 (MQ=255)
                     tAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACAAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCAAggg                                                                                                                                 >  1:3063833/1‑150 (MQ=255)
                      aGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGt                                                                                                                                >  2:1806553/1‑150 (MQ=255)
                       gACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTg                                                                                                                               >  2:5281292/1‑150 (MQ=255)
                        aCCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTgg                                                                                                                              <  1:1796047/150‑1 (MQ=255)
                        aCCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTgg                                                                                                                              <  1:530965/150‑1 (MQ=255)
                              atacatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGc                                                                                                                        >  1:4162326/1‑150 (MQ=255)
                               tacatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGcc                                                                                                                       <  1:258975/150‑1 (MQ=255)
                                acatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATGACTAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGccc                                                                                                                      <  1:2872726/150‑1 (MQ=255)
                                   ttCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGAc                                                                                                                   >  1:1020629/1‑150 (MQ=255)
                                    tCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACt                                                                                                                  <  2:2415812/150‑1 (MQ=255)
                                      aGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTgg                                                                                                                >  1:1987914/1‑150 (MQ=255)
                                       gggAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGt                                                                                                               >  2:1884742/1‑150 (MQ=255)
                                        ggAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGtt                                                                                                              <  2:384119/150‑1 (MQ=255)
                                        ggAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGtt                                                                                                              <  2:1697527/150‑1 (MQ=255)
                                          aaTTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCa                                                                                                            >  2:480071/1‑150 (MQ=255)
                                            ttGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACg                                                                                                          <  1:4102942/150‑1 (MQ=255)
                                            ttGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACg                                                                                                          <  1:480071/150‑1 (MQ=255)
                                              gTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTc                                                                                                        <  1:2494334/150‑1 (MQ=255)
                                                tATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCaa                                                                                                      <  1:1376719/150‑1 (MQ=255)
                                                 aTGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAAt                                                                                                     <  1:4576430/150‑1 (MQ=255)
                                                  tGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATg                                                                                                    <  2:2837615/150‑1 (MQ=255)
                                                  tGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATg                                                                                                    <  2:3006863/150‑1 (MQ=255)
                                                    ttCAAGGAGAGGATGACACCAGATGAACGTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGaa                                                                                                  <  1:4030594/150‑1 (MQ=255)
                                                       aaGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAg                                                                                               >  2:3408628/1‑150 (MQ=255)
                                                       aaGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAg                                                                                               <  2:4111254/150‑1 (MQ=255)
                                                        aGGAGAGGATGACACCAGATGAACGTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAgg                                                                                              <  1:1503267/150‑1 (MQ=255)
                                                         ggAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCTGACCATTAATATATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGTAAAGCCCGACTGGTTCACGTCAATGAACAGGt                                                                                             <  1:3957843/150‑1 (MQ=255)
                                                         ggAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGt                                                                                             >  1:2075932/1‑150 (MQ=255)
                                                          gagaGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAACTGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGtt                                                                                            <  2:5184222/150‑1 (MQ=255)
                                                           agagGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTc                                                                                           <  1:4948852/150‑1 (MQ=255)
                                                            gagGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCg                                                                                          >  1:2536934/1‑150 (MQ=255)
                                                              ggATGACACCAGATGAACTTGCCAGACTGACCGGCTATGGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTg                                                                                        <  2:4314250/150‑1 (MQ=255)
                                                                 tGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAat                                                                                     <  2:2096535/150‑1 (MQ=255)
                                                                 tGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAat                                                                                     <  2:3579638/150‑1 (MQ=255)
                                                                   acacCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatat                                                                                   >  2:828029/1‑150 (MQ=255)
                                                                   acacCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatat                                                                                   >  1:2655950/1‑150 (MQ=255)
                                                                   acacCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatat                                                                                   >  1:2307169/1‑150 (MQ=255)
                                                                   acacCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatat                                                                                   <  1:872430/150‑1 (MQ=255)
                                                                     acCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatatat                                                                                 >  1:3122188/1‑150 (MQ=255)
                                                                       cAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTc                                                                               <  2:2527233/150‑1 (MQ=255)
                                                                       cAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTc                                                                               <  1:3355136/150‑1 (MQ=255)
                                                                       cAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACAATTAATAAATGGGTACGCAAGGAAGGATTGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTc                                                                               <  1:3848222/150‑1 (MQ=255)
                                                                         gATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGc                                                                             >  2:715926/1‑150 (MQ=255)
                                                                            gAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAAt                                                                          <  1:5222660/150‑1 (MQ=255)
                                                                            gAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAAt                                                                          <  2:4244312/150‑1 (MQ=255)
                                                                            gAACGTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAAt                                                                          <  1:4693246/150‑1 (MQ=255)
                                                                                     aGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGt                                                                 >  2:473616/1‑150 (MQ=255)
                                                                                       aCTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGACTATATTCTCACTGCTGAACGTcc                                                               >  1:2746702/1‑150 (MQ=255)
                                                                                       aCTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCAAAACATcc                                                               <  2:2049282/150‑1 (MQ=255)
                                                                                          gACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGa                                                            >  1:1865334/1‑150 (MQ=255)
                                                                                          gACCGGCTATAGCCGCCAGACCATGAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGa                                                            <  2:3508881/150‑1 (MQ=255)
                                                                                               gCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTc                                                       <  1:2320529/150‑1 (MQ=255)
                                                                                                 tataGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAg                                                     <  2:5019776/150‑1 (MQ=255)
                                                                                                  ataGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAgg                                                    >  2:4464477/1‑150 (MQ=255)
                                                                                                      ccgccAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGgaga                                                >  2:1164585/1‑150 (MQ=255)
                                                                                                       cgccAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGaa                                               >  2:1581029/1‑150 (MQ=255)
                                                                                                        gccAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAg                                              <  1:3821127/150‑1 (MQ=255)
                                                                                                         ccAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAgc                                             <  1:3224680/150‑1 (MQ=255)
                                                                                                          cAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAgcg                                            >  2:1197409/1‑150 (MQ=255)
                                                                                                          cAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAgcg                                            >  1:593320/1‑150 (MQ=255)
                                                                                                           aGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAgcgc                                           >  1:3098891/1‑150 (MQ=255)
                                                                                                             aCCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCt                                         <  1:5281461/150‑1 (MQ=255)
                                                                                                              tcATTAATAAATGGGTAAGCAAGGAAGGCTGGTCAAAGTAAACAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTGAATGATCAGGTTCGTTAATATATTCGCAATGCTGAACGTCAAGAAGGTCAGGGTGATGCGCCTg                                        <  2:3183277/149‑1 (MQ=255)
                                                                                                               cATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGc                                       >  1:325256/1‑150 (MQ=255)
                                                                                                                  taataaATGGGTACGCAAGGAAGGATGGACAACGTCACCAAAAACTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCt                                    >  2:1416254/1‑150 (MQ=255)
                                                                                                                    ataaATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCttt                                  >  1:2822697/1‑150 (MQ=255)
                                                                                                                     taaATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTc                                 <  2:5320773/150‑1 (MQ=255)
                                                                                                                      aaaTGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCt                                <  2:2340245/150‑1 (MQ=255)
                                                                                                                                 cAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGcgcc                     <  2:2962748/150‑1 (MQ=255)
                                                                                                                                 cAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTGAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGcgcc                     >  1:4342981/1‑150 (MQ=255)
                                                                                                                                   aggaaggCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGcgccgc                   >  1:4510739/1‑150 (MQ=255)
                                                                                                                                      aaggCTGGACAACGTCACCACAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATAGTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTg                <  2:164169/150‑1 (MQ=255)
                                                                                                                                          cTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGt            <  1:1584732/150‑1 (MQ=255)
                                                                                                                                           tGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTa           >  2:4512343/1‑150 (MQ=255)
                                                                                                                                           tGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTa           >  1:716631/1‑150 (MQ=255)
                                                                                                                                            ggACAACGTCACCAAACCCGGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTAt          <  1:1103282/150‑1 (MQ=255)
                                                                                                                                            ggAAAACGTCAACAAACCCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATTAACAGGTTCGTTAATATATTCGCATTGCTGAACGTCCAGAAGTTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTAt          <  1:3603818/150‑1 (MQ=255)
                                                                                                                                             gACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTAtt         >  2:4174020/1‑150 (MQ=255)
                                                                                                                                                aaCGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCt      <  1:1631933/150‑1 (MQ=255)
                                                                                                                                                 aCGTCACCAAAACCTGTCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg     <  2:3122841/150‑1 (MQ=255)
                                                                                                                                                 aCGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg     <  2:1278460/150‑1 (MQ=255)
                                                                                                                                                   gTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATAGTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGt   <  2:561867/150‑1 (MQ=255)
                                                                                                                                                    tCACCAAACCCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTg  <  1:1255934/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGTTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTG  >  CP009273/2511882‑2512179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A