Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,549,144 C→A 27.2% D206Y (GAT→TAT)  eutL ← putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,549,1440CA27.2% 615.0 / 187.0 290D206Y (GAT→TAT) eutLputative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base C (112/99);  new base A (34/45);  total (146/144)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

CAGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCA  >  CP009273/2548995‑2549293
                                                                                                                                                     |                                                                                                                                                     
cAGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCATTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATc                                                                                                                                                       >  2:1298732/1‑150 (MQ=255)
cAGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATc                                                                                                                                                       <  2:4509362/150‑1 (MQ=255)
 aGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCNGCACTGCATCg                                                                                                                                                      <  1:2767252/150‑1 (MQ=255)
 aGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCg                                                                                                                                                      <  1:3920747/150‑1 (MQ=255)
  gACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCgg                                                                                                                                                     <  2:2158689/150‑1 (MQ=255)
   aCGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCTCTGCGACCATGACGTCCACTTCCAGCAATCCCAGTGCATTGAACATCGGCAACCTCCGTTACGCACGCTGGATTGGATAACGCGCGATTTCCAGCACTGCATCGGt                                                                                                                                                    >  2:4858664/1‑150 (MQ=255)
       aCGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTCTCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAg                                                                                                                                                <  1:3064026/150‑1 (MQ=255)
         gTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGc                                                                                                                                              >  1:4525447/1‑150 (MQ=255)
            aGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGtt                                                                                                                                           >  2:923343/1‑150 (MQ=255)
            aGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGtt                                                                                                                                           >  2:1972273/1‑150 (MQ=255)
            aGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGtt                                                                                                                                           <  1:1479315/150‑1 (MQ=255)
                gctTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTCCGCGCGATTTCCAGCACTGCATCGGTCAAGGCGTTCCAg                                                                                                                                       >  2:2407788/1‑150 (MQ=255)
                 ctTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAgg                                                                                                                                      <  1:345798/150‑1 (MQ=255)
                   ttGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGgct                                                                                                                                    <  2:1164520/150‑1 (MQ=255)
                    tGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGgctg                                                                                                                                   >  2:175621/1‑150 (MQ=255)
                      aGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGgctgct                                                                                                                                 >  2:1335622/1‑150 (MQ=255)
                        cATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCttt                                                                                                                               >  2:2622834/1‑150 (MQ=255)
                        cATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCttt                                                                                                                               <  2:4611670/150‑1 (MQ=255)
                         aTGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTa                                                                                                                              >  2:1650766/1‑150 (MQ=255)
                          tGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTac                                                                                                                             >  2:2754319/1‑150 (MQ=255)
                             cATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACg                                                                                                                          <  1:2013351/150‑1 (MQ=255)
                             cATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACg                                                                                                                          >  1:2326772/1‑150 (MQ=255)
                              aTCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGc                                                                                                                         >  2:1445003/1‑150 (MQ=255)
                              aTCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGc                                                                                                                         >  1:1052081/1‑150 (MQ=255)
                                ccGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTg                                                                                                                       <  1:4059683/150‑1 (MQ=255)
                                ccGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTg                                                                                                                       <  2:384687/150‑1 (MQ=255)
                                 cGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGc                                                                                                                      >  2:3136541/1‑150 (MQ=255)
                                  gCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTCCGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGcc                                                                                                                     >  1:2627438/1‑150 (MQ=255)
                                  gCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGcc                                                                                                                     <  2:851500/150‑1 (MQ=255)
                                   cTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACAGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCt                                                                                                                    <  1:1531448/150‑1 (MQ=255)
                                     gCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTgg                                                                                                                  >  1:3804669/1‑150 (MQ=255)
                                     gCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTgg                                                                                                                  >  1:1872667/1‑150 (MQ=255)
                                       aTCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCt                                                                                                                >  2:3436269/1‑150 (MQ=255)
                                        tCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTa                                                                                                               <  1:2429326/150‑1 (MQ=255)
                                              gcggcgACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGtt                                                                                                         <  1:1959211/150‑1 (MQ=255)
                                              gcggcgACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGtt                                                                                                         <  1:3689602/150‑1 (MQ=255)
                                               cggcgACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTa                                                                                                        <  2:1690572/150‑1 (MQ=255)
                                                ggcgACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTaa                                                                                                       >  1:647171/1‑150 (MQ=255)
                                                    aCCATGCCGTCCTCTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCTGTATAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAt                                                                                                   <  2:1141554/150‑1 (MQ=255)
                                                    aCCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAt                                                                                                   >  1:4869657/1‑150 (MQ=255)
                                                       aTGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtgct                                                                                                <  1:2117648/150‑1 (MQ=255)
                                                        tGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtgctg                                                                                               >  2:5328201/1‑150 (MQ=255)
                                                          ccGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGcc                                                                                             <  1:3956660/150‑1 (MQ=255)
                                                          ccGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGcc                                                                                             <  1:966950/150‑1 (MQ=255)
                                                              ccACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGagt                                                                                         >  2:1807287/1‑150 (MQ=255)
                                                                 cTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGagtagt                                                                                      >  1:1182025/1‑150 (MQ=255)
                                                                  ttCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGtt                                                                                     >  1:4385250/1‑150 (MQ=255)
                                                                     cAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGt                                                                                  <  2:3026037/150‑1 (MQ=255)
                                                                       gCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGttt                                                                                <  2:4368375/150‑1 (MQ=255)
                                                                        cAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTc                                                                               <  1:4375263/150‑1 (MQ=255)
                                                                        cAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTc                                                                               <  1:2262357/150‑1 (MQ=255)
                                                                         aaTCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCa                                                                              <  2:3661492/150‑1 (MQ=255)
                                                                         aaTCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCa                                                                              >  1:4357625/1‑150 (MQ=255)
                                                                             ccAGAGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTCCGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACg                                                                          <  2:382011/150‑1 (MQ=255)
                                                                              cAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACgg                                                                         <  1:1141661/150‑1 (MQ=255)
                                                                              cAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACgg                                                                         <  1:263837/150‑1 (MQ=255)
                                                                               aGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGt                                                                        <  2:2003801/150‑1 (MQ=255)
                                                                                gTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTg                                                                       <  1:3535072/150‑1 (MQ=255)
                                                                                  gCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGc                                                                     <  2:4139089/150‑1 (MQ=255)
                                                                                  gCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGCAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGc                                                                     <  2:1515687/150‑1 (MQ=255)
                                                                                   cATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCg                                                                    >  1:3739668/1‑150 (MQ=255)
                                                                                      tGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGa                                                                 >  1:2594714/1‑150 (MQ=255)
                                                                                       gATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGAc                                                                >  2:5068217/1‑150 (MQ=255)
                                                                                        atcatcGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACa                                                               >  2:3218057/1‑150 (MQ=255)
                                                                                         tcatcGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACAt                                                              <  2:4055517/150‑1 (MQ=255)
                                                                                           atcGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAg                                                            <  1:4468593/150‑1 (MQ=255)
                                                                                              ggCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTc                                                         >  1:469025/1‑150 (MQ=255)
                                                                                                 aaCCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGcc                                                      >  1:4595159/1‑150 (MQ=255)
                                                                                                     tCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCt                                                  >  2:4750246/1‑150 (MQ=255)
                                                                                                      ccGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTg                                                 <  1:861385/150‑1 (MQ=255)
                                                                                                         ttACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTAc                                              >  1:5066051/1‑150 (MQ=255)
                                                                                                         ttACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACAGAGGTCGCCAGCTGTAc                                              >  1:4330031/1‑150 (MQ=255)
                                                                                                            cgcacgcTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTc                                           >  1:2800982/1‑150 (MQ=255)
                                                                                                             gcacgcTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCg                                          <  1:4451520/150‑1 (MQ=255)
                                                                                                             gcacgcTGGATTGGATTACGCGCGATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTTCCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCg                                          <  1:1898288/150‑1 (MQ=255)
                                                                                                               acgcTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGc                                        >  2:5241603/1‑150 (MQ=255)
                                                                                                                  cTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGa                                     >  2:1167444/1‑150 (MQ=255)
                                                                                                                    ggattggattACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACt                                   <  2:351456/150‑1 (MQ=255)
                                                                                                                      attggattACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTc                                 >  2:2680286/1‑150 (MQ=255)
                                                                                                                        tggattACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAg                               >  1:1217229/1‑150 (MQ=255)
                                                                                                                            ttACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCt                           >  2:2243573/1‑150 (MQ=255)
                                                                                                                               cgcgcgATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCa                        <  1:580084/150‑1 (MQ=255)
                                                                                                                                gcgcgATTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCAt                       <  1:1415187/150‑1 (MQ=255)
                                                                                                                                   cgATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCaa                    >  2:3688975/1‑150 (MQ=255)
                                                                                                                                     aTTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATg                  <  2:3928396/150‑1 (MQ=255)
                                                                                                                                     aTTTCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATg                  >  2:4231042/1‑150 (MQ=255)
                                                                                                                                      tttCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGc                 >  1:3884246/1‑150 (MQ=255)
                                                                                                                                      tttCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGc                 <  1:3136103/150‑1 (MQ=255)
                                                                                                                                       ttCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGcc                <  1:2825520/150‑1 (MQ=255)
                                                                                                                                        tCCAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCa               >  2:2731129/1‑150 (MQ=255)
                                                                                                                                         ccAGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCAt              >  1:1252055/1‑150 (MQ=255)
                                                                                                                                           aGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAg            >  1:1009880/1‑150 (MQ=255)
                                                                                                                                           aGCACTGCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAg            >  2:737436/1‑150 (MQ=255)
                                                                                                                                            gCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAgg           <  1:3371760/150‑1 (MQ=255)
                                                                                                                                            gCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAgg           <  2:2456254/150‑1 (MQ=255)
                                                                                                                                               cTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGGTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCg        >  2:335357/1‑150 (MQ=255)
                                                                                                                                                 gCATAGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCt      <  2:2560047/150‑1 (MQ=255)
                                                                                                                                                   aTCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTc    <  1:2727089/150‑1 (MQ=255)
                                                                                                                                                    tCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTcc   <  1:175621/150‑1 (MQ=255)
                                                                                                                                                     cGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCa  <  2:2326772/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGACGAACGTTAGCTGCTTTGAGCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCA  >  CP009273/2548995‑2549293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A