Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,143,969 G→T 27.4% intergenic (+64/+208) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,143,9690GT27.4% 554.8 / 170.6 266intergenic (+64/+208)yghA/exbDputative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base G (88/105);  new base T (30/43);  total (118/148)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 271 total aligned reads displayed.

GTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTC  >  CP009273/3143822‑3144118
                                                                                                                                                   |                                                                                                                                                     
gTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGaa                                                                                                                                                     >  2:4395898/1‑150 (MQ=255)
gTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGaa                                                                                                                                                     >  2:4212937/1‑150 (MQ=255)
  atatGTTTATCTGGCTAGTCAGGAGTCGAGCTACGTCACCGCAGACGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGaaca                                                                                                                                                   <  2:335484/150‑3 (MQ=255)
   tatGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAAc                                                                                                                                                  <  2:3943205/150‑1 (MQ=255)
   tatGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAAc                                                                                                                                                  <  2:4252376/150‑1 (MQ=255)
   tatGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAAc                                                                                                                                                  >  1:920609/1‑150 (MQ=255)
      gTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGcc                                                                                                                                               >  2:4124672/1‑150 (MQ=255)
        ttATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCtt                                                                                                                                             <  1:1297442/150‑1 (MQ=255)
         tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTa                                                                                                                                            <  1:3409064/150‑1 (MQ=255)
         tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGAGGTGGCGAAAACGCCTTa                                                                                                                                            <  2:1705007/150‑1 (MQ=255)
         tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTGACCTGATGTGGCTAAAACGCCTTa                                                                                                                                            <  2:3756874/150‑1 (MQ=255)
          aTCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGGTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTAt                                                                                                                                           >  2:3075638/1‑150 (MQ=255)
            cTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATcc                                                                                                                                         <  2:2600787/150‑1 (MQ=255)
            cTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATcc                                                                                                                                         <  2:2761425/150‑1 (MQ=255)
             tGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCg                                                                                                                                        >  2:2653867/1‑150 (MQ=255)
                 aaGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTa                                                                                                                                    >  1:4281954/1‑150 (MQ=255)
                    tCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACaa                                                                                                                                 >  1:1637126/1‑150 (MQ=255)
                     cAGGAGTCGAGCTACGTCACCGCAGTAGTGCACGGCGTGTGCGGCGGCGAGCAGTTTGGTTAAATATATGCCCGGTTGTGAAAAGCAACCGGGCATCAGTGTGATTTCACGCGTCCCTGATGTGGCTAGAACGCCTGAGCCGCCTACaca                                                                                                                                >  2:4509804/1‑148 (MQ=255)
                      aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAAc                                                                                                                               >  1:4190348/1‑150 (MQ=255)
                      aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAAc                                                                                                                               >  2:14846/1‑150 (MQ=255)
                      aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAAc                                                                                                                               <  1:2802610/150‑1 (MQ=255)
                      aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAAc                                                                                                                               <  1:5051382/150‑1 (MQ=255)
                          gTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCAt                                                                                                                           <  2:4960621/150‑1 (MQ=255)
                          gTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCAt                                                                                                                           <  2:2057958/150‑1 (MQ=255)
                           tCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATg                                                                                                                          <  2:5212941/150‑1 (MQ=255)
                              aGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAg                                                                                                                       <  2:5294399/150‑1 (MQ=255)
                              aGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAg                                                                                                                       <  1:4116743/150‑1 (MQ=255)
                                cTACGTCACCGCAGACGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCACCCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGAt                                                                                                                     >  2:4092075/1‑150 (MQ=255)
                                cTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGAt                                                                                                                     <  2:250161/150‑1 (MQ=255)
                                   cGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCa                                                                                                                  <  2:1058130/150‑1 (MQ=255)
                                     tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAAc                                                                                                                <  1:2737770/150‑1 (MQ=255)
                                     tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAAc                                                                                                                <  1:1725428/150‑1 (MQ=255)
                                     tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAAc                                                                                                                <  2:3290629/150‑1 (MQ=255)
                                           cAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTg                                                                                                          <  2:1290872/150‑1 (MQ=255)
                                             gAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCa                                                                                                        <  2:2841274/150‑1 (MQ=255)
                                             gAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCa                                                                                                        <  1:1267328/150‑1 (MQ=255)
                                                 tGCACGGCGTGAGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAACCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAg                                                                                                    <  2:2957364/150‑1 (MQ=255)
                                                   cACGGCGTGTGCGGCGGCGAGCATTTATGTTAAAAATATGCCCGGTTGTGATAAGCAACCGGGCATCATTGTGAATTCTCTCTTTCCTGATGTGGCGAAATCGCCTTATCCGACTACAAACGCATGAAGATTCAACATGTTGCAATAGAt                                                                                                  <  2:58088/150‑1 (MQ=38)
                                                      ggCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGAtttt                                                                                               <  2:2090962/150‑1 (MQ=255)
                                                       gCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTg                                                                                              <  1:4766133/150‑1 (MQ=255)
                                                       gCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTg                                                                                              <  2:4583803/150‑1 (MQ=255)
                                                         gtgtGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTa                                                                                            >  1:5148748/1‑150 (MQ=255)
                                                         gtgtGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTa                                                                                            <  2:3208192/150‑1 (MQ=255)
                                                          tgtgCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAg                                                                                           <  1:3575972/150‑1 (MQ=255)
                                                          tgtgCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAg                                                                                           <  2:746553/150‑1 (MQ=255)
                                                            tgCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGc                                                                                         <  2:1581782/150‑1 (MQ=255)
                                                                gcggcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGa                                                                                     <  2:1884370/150‑1 (MQ=255)
                                                                 cggcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCATCCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGAt                                                                                    <  1:2979894/150‑1 (MQ=255)
                                                                 cggcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGAt                                                                                    <  1:1936051/150‑1 (MQ=255)
                                                                   gcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATaa                                                                                  >  1:5004496/1‑150 (MQ=255)
                                                                   gcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCGTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATaa                                                                                  <  2:1167472/150‑1 (MQ=255)
                                                                       gCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGAATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGt                                                                              <  2:126942/150‑1 (MQ=255)
                                                                        cATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTa                                                                             <  1:1195426/150‑1 (MQ=255)
                                                                          tttAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAgc                                                                           >  1:1918301/1‑150 (MQ=255)
                                                                           ttAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAgcg                                                                          <  2:3099274/150‑1 (MQ=255)
                                                                           ttAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAgcg                                                                          <  1:2265502/150‑1 (MQ=255)
                                                                           ttAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAgcg                                                                          <  1:3666236/150‑1 (MQ=255)
                                                                            tAGGTTAAAAAAATGCCCGGTTGTGAAAAGCACCCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAgcgc                                                                         >  2:2219738/1‑150 (MQ=255)
                                                                              ggTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCAt                                                                       >  1:4372903/1‑150 (MQ=255)
                                                                               gTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAACACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATc                                                                      >  1:334284/1‑150 (MQ=255)
                                                                               gTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATc                                                                      >  2:3090427/1‑150 (MQ=255)
                                                                               gTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATc                                                                      >  1:2030400/1‑150 (MQ=255)
                                                                                 tAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                    <  2:350436/150‑1 (MQ=255)
                                                                                      aaaTGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                               <  2:1954452/150‑1 (MQ=255)
                                                                                        aTGCCCGGTTGTGAAAAGCAACCGGGCATCAGTGTGAATTCCCTCTTACCTGATGTGGCGAAAACGCCGTATCCGCCTACAAACGCAGGAAGATTCAACAGGGTGCAATAGAGTGTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAGTGa                                                             >  2:3595445/1‑150 (MQ=38)
                                                                                         tGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAg                                                            <  2:1826532/150‑1 (MQ=255)
                                                                                         tGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATTTTGCAATATATTTTGTAGGCCTGATAAGCGTAGCTCATCAGGCATTGAg                                                            >  2:4900618/1‑150 (MQ=255)
                                                                                         tGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAg                                                            <  1:69083/150‑1 (MQ=255)
                                                                                           cccGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCa                                                          <  2:5099342/150‑1 (MQ=255)
                                                                                           cccGGTGGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCa                                                          >  2:906975/1‑150 (MQ=255)
                                                                                                 tgtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAAt                                                    >  2:2187244/1‑150 (MQ=255)
                                                                                                 tgtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAAt                                                    <  1:5301919/150‑1 (MQ=255)
                                                                                                 tgtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAAt                                                    <  1:4696523/150‑1 (MQ=255)
                                                                                                  gtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATg                                                   <  1:4003537/150‑1 (MQ=255)
                                                                                                  gtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATg                                                   <  2:4673846/150‑1 (MQ=255)
                                                                                                         gCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATg                                            <  1:1944903/150‑1 (MQ=255)
                                                                                                          cAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGc                                           >  1:2469885/1‑150 (MQ=255)
                                                                                                            aCCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcgt                                         <  2:4444964/150‑1 (MQ=255)
                                                                                                              cGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcgtcg                                       >  2:1323546/1‑150 (MQ=255)
                                                                                                                  catcatTGTGAATTCACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGaa                                   >  1:944628/1‑150 (MQ=255)
                                                                                                                   atcatTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAAc                                  >  2:1751504/1‑150 (MQ=255)
                                                                                                                      atTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGcc                               >  2:367415/1‑150 (MQ=255)
                                                                                                                      atTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGcc                               >  1:4518354/1‑150 (MQ=255)
                                                                                                                      atTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGcc                               >  2:4828426/1‑150 (MQ=255)
                                                                                                                            aagtCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATcc                         <  1:4486562/147‑1 (MQ=255)
                                                                                                                            aaTTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATcc                         <  1:906975/150‑1 (MQ=255)
                                                                                                                                cACTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGcc                     <  2:2379720/150‑1 (MQ=255)
                                                                                                                                 aCTCTTACCTGATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCt                    >  1:2574511/1‑150 (MQ=255)
                                                                                                                                  ctctTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTa                   >  1:2210904/1‑150 (MQ=255)
                                                                                                                                  ctctTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTa                   >  1:3474248/1‑150 (MQ=255)
                                                                                                                                    ctTCCCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCATGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACa                 <  2:1015525/150‑1 (MQ=255)
                                                                                                                                      tACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACaaa               >  1:585062/1‑150 (MQ=255)
                                                                                                                                           gATGTGGCTAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc          <  1:14846/150‑1 (MQ=255)
                                                                                                                                           gATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc          <  1:1110810/150‑1 (MQ=255)
                                                                                                                                           gATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc          >  1:4472120/1‑150 (MQ=255)
                                                                                                                                                 gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGAt    <  1:123879/150‑1 (MQ=255)
                                                                                                                                                   gAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTc  >  1:3697723/1‑150 (MQ=255)
                                                                                                                                                   gAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTc  >  1:1024947/1‑150 (MQ=255)
                                                                                                                                                   gAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTc  <  2:2508412/150‑1 (MQ=255)
                                                                                                                                                   gAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTc  >  2:2785422/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTC  >  CP009273/3143822‑3144118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A