Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,289,247 G→A 57.5% intergenic (‑64/+134) fdhF ← / ← mdtP formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,289,2470GA57.5% 114.8 / inf 265intergenic (‑64/+134)fdhF/mdtPformate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (50/62);  new base A (65/87);  total (115/150)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 271 total aligned reads displayed.

GCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGAC  >  CP009273/4289098‑4289395
                                                                                                                                                     |                                                                                                                                                    
  ccGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGcc                                                                                                                                                    <  2:2812760/150‑1 (MQ=255)
    ggACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCcac                                                                                                                                                  >  2:3410729/1‑150 (MQ=255)
      aCGATTGTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACAt                                                                                                                                                <  2:239011/150‑1 (MQ=255)
        gATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACAttt                                                                                                                                              <  1:2628274/150‑1 (MQ=255)
            ttGCCTTTATCGACGACCATGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTAtg                                                                                                                                          <  2:2464937/150‑1 (MQ=255)
            gtGCCGTTATCGACGACCAGGTTGATTTTGCAACCTTATGCGCTATAGGGGCAAACCGTGACGTCTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCTCTCTAGACTACAGTATGCATCTTTTATGCCACATTTTAtg                                                                                                                                          <  2:4385721/149‑1 (MQ=255)
             tGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTAtgt                                                                                                                                         <  2:182443/150‑1 (MQ=255)
              gCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTAtgtg                                                                                                                                        <  1:1590159/150‑1 (MQ=255)
              gCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTAtgtg                                                                                                                                        <  2:4047407/150‑1 (MQ=255)
                cGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTggg                                                                                                                                      <  2:3171569/150‑1 (MQ=255)
                 gTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTgggg                                                                                                                                     >  2:738137/1‑150 (MQ=255)
                    aTCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTCTGTGGGGTCa                                                                                                                                  >  2:4537699/1‑150 (MQ=255)
                      cgacgaCCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCAtt                                                                                                                                >  1:5282573/1‑150 (MQ=255)
                       gacgacCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTc                                                                                                                               >  1:3791629/1‑150 (MQ=255)
                        acgacCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTcc                                                                                                                              <  1:2443836/150‑1 (MQ=255)
                          gacCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCt                                                                                                                            <  1:1145777/150‑1 (MQ=255)
                           acCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTg                                                                                                                           <  1:1013057/150‑1 (MQ=255)
                            ccAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGa                                                                                                                          >  1:1263178/1‑150 (MQ=255)
                            ccAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGa                                                                                                                          >  1:3921101/1‑150 (MQ=255)
                             cAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGat                                                                                                                         <  1:2391168/150‑1 (MQ=255)
                               ggTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGatat                                                                                                                       <  2:4027674/150‑1 (MQ=255)
                               ggTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGatat                                                                                                                       <  1:737143/150‑1 (MQ=255)
                                gTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATAtt                                                                                                                      <  2:4168884/150‑1 (MQ=255)
                                  tGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTAc                                                                                                                    <  2:4094267/150‑1 (MQ=255)
                                      tttGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGc                                                                                                                >  1:3062494/1‑150 (MQ=255)
                                       ttGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCa                                                                                                               >  2:3936099/1‑150 (MQ=255)
                                         gCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACt                                                                                                             >  1:1304050/1‑150 (MQ=255)
                                           aaCCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACtat                                                                                                           >  2:2488380/1‑150 (MQ=255)
                                           aaCCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACtat                                                                                                           <  1:3710188/150‑1 (MQ=255)
                                           aaCCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACtat                                                                                                           <  1:1166709/150‑1 (MQ=255)
                                           aaCCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACtat                                                                                                           >  1:3771112/1‑150 (MQ=255)
                                             ccTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTGAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACtattt                                                                                                         <  2:2012551/150‑1 (MQ=255)
                                                  tGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCa                                                                                                    <  2:1445090/150‑1 (MQ=255)
                                                   gcgcAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCaa                                                                                                   <  2:3317990/150‑1 (MQ=255)
                                                   gcgcAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCaa                                                                                                   <  2:1382123/150‑1 (MQ=255)
                                                     gcAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCaaaa                                                                                                 >  2:3890856/1‑150 (MQ=255)
                                                      cAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAc                                                                                                >  2:1378473/1‑150 (MQ=255)
                                                       aaTAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCTCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAct                                                                                               >  1:847702/1‑150 (MQ=255)
                                                       aaTAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAct                                                                                               <  2:3024/150‑1 (MQ=255)
                                                       aaTAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAct                                                                                               <  1:4544672/150‑1 (MQ=255)
                                                         tAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAActct                                                                                             <  2:4720202/150‑1 (MQ=255)
                                                            gggCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGAc                                                                                          <  2:2015054/150‑1 (MQ=255)
                                                             ggCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACg                                                                                         <  1:3457753/150‑1 (MQ=255)
                                                             ggCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACg                                                                                         <  1:1624190/150‑1 (MQ=255)
                                                               cAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGaa                                                                                       >  2:4769778/1‑150 (MQ=255)
                                                                aaaCCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGaaa                                                                                      >  2:2587515/1‑150 (MQ=255)
                                                                aaaCCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGaaa                                                                                      <  1:1703914/150‑1 (MQ=255)
                                                                    cGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACa                                                                                  >  2:3234315/1‑150 (MQ=255)
                                                                    cGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACa                                                                                  >  2:2968888/1‑150 (MQ=255)
                                                                    cGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTGTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACa                                                                                  >  2:4876679/1‑150 (MQ=255)
                                                                     gTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAg                                                                                 <  1:3276981/150‑1 (MQ=255)
                                                                      tGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAgg                                                                                >  2:3987299/1‑150 (MQ=255)
                                                                       aacgacTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAATCAGGc                                                                               >  2:703354/2‑150 (MQ=255)
                                                                           acTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgtc                                                                           <  1:960055/150‑1 (MQ=255)
                                                                                  tCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGtt                                                                    >  2:4815332/1‑150 (MQ=255)
                                                                                  tCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGtt                                                                    <  1:3830218/150‑1 (MQ=255)
                                                                                  tCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGtt                                                                    >  2:2169402/1‑150 (MQ=255)
                                                                                       ggTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTtgac                                                               >  1:5055289/1‑150 (MQ=255)
                                                                                       ggTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTtgac                                                               <  1:3012499/150‑1 (MQ=255)
                                                                                          ctcGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTtgacgtg                                                            <  1:3182138/150‑1 (MQ=255)
                                                                                          ctcGCTCCAGTTAATCAAATCACGCAGACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTtgacgtg                                                            <  2:3516828/150‑1 (MQ=255)
                                                                                             gCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTGTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTtgacgtgacg                                                         >  2:4253602/1‑150 (MQ=255)
                                                                                               tCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGacgaa                                                       <  2:1705681/150‑1 (MQ=255)
                                                                                                    ttAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGacgaaacgaa                                                  <  1:4044733/150‑1 (MQ=255)
                                                                                                      aatcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAAt                                                <  2:4442431/150‑1 (MQ=255)
                                                                                                      aatcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAAt                                                >  1:3355286/1‑150 (MQ=255)
                                                                                                       atcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATa                                               <  2:1864632/150‑1 (MQ=255)
                                                                                                        tcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATaa                                              >  2:1237233/1‑149 (MQ=255)
                                                                                                         caaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATAcc                                             <  1:1493107/150‑1 (MQ=255)
                                                                                                         caaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATAcc                                             <  1:2792821/150‑1 (MQ=255)
                                                                                                          aaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACcg                                            <  1:4696276/150‑1 (MQ=255)
                                                                                                          aaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACcg                                            >  2:4499996/1‑150 (MQ=255)
                                                                                                                cGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATAGTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGAc                                      <  2:796940/150‑1 (MQ=255)
                                                                                                                 gCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACa                                     <  1:4000841/150‑1 (MQ=255)
                                                                                                                     aCGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCa                                 >  1:43623/1‑150 (MQ=255)
                                                                                                                      cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAt                                <  1:2801560/150‑1 (MQ=255)
                                                                                                                       gcgcTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATc                               <  1:2120437/150‑1 (MQ=255)
                                                                                                                       gcgcTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATc                               <  1:4771708/150‑1 (MQ=255)
                                                                                                                        cgcTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCa                              <  2:1545131/150‑1 (MQ=255)
                                                                                                                        cgcTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACTGGCACTATTTATTCAAAACTCTGAAGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGAGTGACAGCCATCa                              >  2:5085695/1‑150 (MQ=255)
                                                                                                                         gcTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAc                             >  1:2466542/1‑150 (MQ=255)
                                                                                                                           tctcGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcgc                           >  2:2296901/1‑150 (MQ=255)
                                                                                                                           tctcGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcgc                           >  1:335786/1‑150 (MQ=255)
                                                                                                                              cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGc                        <  2:2941414/150‑1 (MQ=255)
                                                                                                                               gACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCa                       <  2:3811598/150‑1 (MQ=255)
                                                                                                                               gACTACAGTATGCATCTTTTATAACACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGACACGAAATACCGCGTGACAGCCATCACGCGGCa                       >  2:3041623/1‑150 (MQ=255)
                                                                                                                                aCTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAg                      <  1:4950170/150‑1 (MQ=255)
                                                                                                                                 cTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGa                     >  2:2232535/1‑150 (MQ=255)
                                                                                                                                   aCAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACa                   <  2:4490831/150‑1 (MQ=255)
                                                                                                                                    cAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACat                  <  1:4679014/150‑1 (MQ=255)
                                                                                                                                        aTGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACatttta              >  2:3367541/1‑150 (MQ=255)
                                                                                                                                           cATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt           <  2:2674390/150‑1 (MQ=255)
                                                                                                                                           cATATTTTATAACACATTTTATGTGGGGTCATTCCCTGATATTACTGGCACTATTTATTCAAAACTCTGACGAAAAACAGGATTACGTCAGTTTTGACGTGACGATACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt           <  1:2005626/150‑1 (MQ=255)
                                                                                                                                             tCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTAttttt         <  1:886505/150‑1 (MQ=255)
                                                                                                                                                tttATACCACTTTTTATGTGGGGTCATTCCCTGATATTACGGGCACTTTTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAATCGAAATACCGCGTGACAGCCATCTCGCGGCAGACATTTTATTTTTtct      <  2:4407886/150‑1 (MQ=255)
                                                                                                                                                  ttggCCACATTTTCTGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTGGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg    <  1:4478933/147‑1 (MQ=255)
                                                                                                                                                  tataCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg    >  2:2068054/1‑150 (MQ=255)
                                                                                                                                                  tataCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg    <  1:2228660/150‑1 (MQ=255)
                                                                                                                                                  tATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg    >  2:1098985/1‑150 (MQ=255)
                                                                                                                                                    tGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgac  >  1:1794408/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGAC  >  CP009273/4289098‑4289395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGAC  >  CP009273/4289099‑4289395
                                                                                                                                                    |                                                                                                                                                    
CCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATACATCTTTTATAC                                                                                                                                                     >  SRR19973062.609638/1‑150 (MQ=60)
 CCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCC                                                                                                                                                    >  SRR19973062.4465089/1‑150 (MQ=60)
 CCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACC                                                                                                                                                    >  SRR19973062.3064156/1‑150 (MQ=60)
  CGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCA                                                                                                                                                   >  SRR19973062.785241/1‑150 (MQ=60)
  CGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCA                                                                                                                                                   <  SRR19973062.1964589/150‑1 (MQ=60)
  CGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCGTTTATACCA                                                                                                                                                   >  SRR19973062.4009061/1‑150 (MQ=60)
       GATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTT                                                                                                                                              <  SRR19973062.2642122/150‑1 (MQ=60)
       GATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTT                                                                                                                                              >  SRR19973062.3597952/1‑150 (MQ=60)
          TTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTAT                                                                                                                                           <  SRR19973062.503561/150‑1 (MQ=60)
          TTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTAT                                                                                                                                           >  SRR19973062.920436/1‑150 (MQ=60)
          TTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTAT                                                                                                                                           <  SRR19973062.518330/150‑1 (MQ=60)
           TTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATG                                                                                                                                          <  SRR19973062.4421523/150‑1 (MQ=60)
            TGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGT                                                                                                                                         <  SRR19973062.5226557/150‑1 (MQ=60)
             GCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTG                                                                                                                                        <  SRR19973062.1598039/150‑1 (MQ=60)
             GCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTG                                                                                                                                        <  SRR19973062.1597965/150‑1 (MQ=60)
             GCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTG                                                                                                                                        <  SRR19973062.171128/150‑1 (MQ=60)
               CGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGG                                                                                                                                      <  SRR19973062.997603/150‑1 (MQ=60)
                GTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGG                                                                                                                                     >  SRR19973062.1186165/1‑150 (MQ=60)
                 TTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGT                                                                                                                                    >  SRR19973062.872626/1‑150 (MQ=60)
                 TTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACACGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGT                                                                                                                                    >  SRR19973062.246796/1‑150 (MQ=60)
                   ATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGTCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGACACATTTTATGTGGGGTCA                                                                                                                                  >  SRR19973062.1170129/1‑150 (MQ=60)
                   ATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCA                                                                                                                                  >  SRR19973062.2479189/1‑150 (MQ=60)
                     CGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATT                                                                                                                                >  SRR19973062.4168337/1‑150 (MQ=60)
                     CGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATT                                                                                                                                >  SRR19973062.5310348/1‑150 (MQ=60)
                      GACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTC                                                                                                                               >  SRR19973062.3811050/1‑150 (MQ=60)
                      GACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTC                                                                                                                               >  SRR19973062.3811186/1‑150 (MQ=60)
                      GACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTC                                                                                                                               >  SRR19973062.3811044/1‑150 (MQ=60)
                       ACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCC                                                                                                                              <  SRR19973062.2456947/150‑1 (MQ=60)
                         GACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCT                                                                                                                            <  SRR19973062.1151144/150‑1 (MQ=60)
                         GACCAGGTTGATTTTGCAACCTGATGCGCAAGAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCT                                                                                                                            <  SRR19973062.873907/150‑1 (MQ=60)
                          ACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTG                                                                                                                           <  SRR19973062.1017785/150‑1 (MQ=60)
                           CCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGA                                                                                                                          <  SRR19973062.4631929/150‑1 (MQ=60)
                           CCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGA                                                                                                                          >  SRR19973062.1268999/1‑150 (MQ=60)
                           CCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGA                                                                                                                          >  SRR19973062.3941050/1‑150 (MQ=60)
                            CAGGTTGATTTTGCAACCTGATGCGCAATAGGTGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATTCCACATTTTATGTGGGGTCATTCCCTGAT                                                                                                                         <  SRR19973062.2403908/150‑1 (MQ=60)
                            CAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGAT                                                                                                                         <  SRR19973062.2403991/150‑1 (MQ=60)
                             AGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATA                                                                                                                        >  SRR19973062.2710066/1‑150 (MQ=60)
                              GGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATAT                                                                                                                       <  SRR19973062.740490/150‑1 (MQ=60)
                               GTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATT                                                                                                                      <  SRR19973062.4707204/150‑1 (MQ=60)
                               GTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATT                                                                                                                      <  SRR19973062.3364379/150‑1 (MQ=60)
                                     TTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGC                                                                                                                >  SRR19973062.3078380/1‑150 (MQ=60)
                                        GCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACT                                                                                                             >  SRR19973062.1310018/1‑150 (MQ=60)
                                         CAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTA                                                                                                            >  SRR19973062.4793333/1‑150 (MQ=60)
                                          AACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTAT                                                                                                           <  SRR19973062.3729237/150‑1 (MQ=60)
                                          AACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTAT                                                                                                           >  SRR19973062.3790436/1‑150 (MQ=60)
                                          AACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTAT                                                                                                           <  SRR19973062.1172180/150‑1 (MQ=60)
                                          AACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTAT                                                                                                           >  SRR19973062.3790435/1‑150 (MQ=60)
                                             CTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTA                                                                                                        <  SRR19973062.1430418/150‑1 (MQ=60)
                                              TGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTAT                                                                                                       <  SRR19973062.4151578/150‑1 (MQ=60)
                                                ATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTC                                                                                                     >  SRR19973062.4981611/1‑150 (MQ=60)
                                                  GCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAA                                                                                                   <  SRR19973062.2701479/150‑1 (MQ=60)
                                                      AATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCTCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACT                                                                                               >  SRR19973062.851371/1‑150 (MQ=60)
                                                      AATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACT                                                                                               <  SRR19973062.4568362/150‑1 (MQ=60)
                                                      AATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACT                                                                                               <  SRR19973062.4568338/150‑1 (MQ=60)
                                                         AGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTG                                                                                            >  SRR19973062.3303922/1‑150 (MQ=60)
                                                            GGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACG                                                                                         <  SRR19973062.3475377/150‑1 (MQ=60)
                                                            GGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACG                                                                                         <  SRR19973062.1631782/150‑1 (MQ=60)
                                                            GGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACG                                                                                         <  SRR19973062.1632221/150‑1 (MQ=60)
                                                              CAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAA                                                                                       >  SRR19973062.4598818/1‑150 (MQ=60)
                                                              CAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAA                                                                                       >  SRR19973062.1672688/1‑150 (MQ=60)
                                                               AAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAA                                                                                      >  SRR19973062.1731745/1‑150 (MQ=60)
                                                               AAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAA                                                                                      <  SRR19973062.1712256/150‑1 (MQ=60)
                                                                  CAGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAAC                                                                                   <  SRR19973062.2029488/150‑1 (MQ=60)
                                                                    GTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAG                                                                                 <  SRR19973062.3293965/150‑1 (MQ=60)
                                                                        CGACTTTTTTCATCGGTCTCGCTCCAGTTATTCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTG                                                                             >  SRR19973062.2842524/1‑150 (MQ=60)
                                                                          ACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTC                                                                           <  SRR19973062.964463/150‑1 (MQ=60)
                                                                           CTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCG                                                                          >  SRR19973062.2423822/1‑150 (MQ=60)
                                                                              TTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCA                                                                       <  SRR19973062.97188/150‑1 (MQ=60)
                                                                                 TCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTT                                                                    <  SRR19973062.3849818/150‑1 (MQ=60)
                                                                                      GGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGAC                                                               >  SRR19973062.5082085/1‑150 (MQ=60)
                                                                                      GGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGAC                                                               <  SRR19973062.3028240/150‑1 (MQ=60)
                                                                                         CTCGCTCCAGTTAATCAACTCACGCCTACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTG                                                            <  SRR19973062.3198640/150‑1 (MQ=60)
                                                                                         CTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTG                                                            <  SRR19973062.3786605/150‑1 (MQ=60)
                                                                                         CTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTG                                                            <  SRR19973062.3198638/150‑1 (MQ=60)
                                                                                         CTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTG                                                            >  SRR19973062.3150560/1‑150 (MQ=60)
                                                                                          TCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATTCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGA                                                           <  SRR19973062.1464695/150‑1 (MQ=60)
                                                                                           CGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGAC                                                          >  SRR19973062.1273391/1‑150 (MQ=60)
                                                                                           AGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGATAAACAGGCTGTCGTCAGTTTTGACGTGAC                                                          >  SRR19973062.3648187/1‑150 (MQ=60)
                                                                                              TCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAA                                                       <  SRR19973062.3428199/150‑1 (MQ=60)
                                                                                                CAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAAC                                                     <  SRR19973062.3918416/150‑1 (MQ=60)
                                                                                                 AGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACG                                                    >  SRR19973062.4887285/1‑150 (MQ=60)
                                                                                                 AGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACG                                                    <  SRR19973062.741487/150‑1 (MQ=60)
                                                                                                   TTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAA                                                  <  SRR19973062.4065384/150‑1 (MQ=60)
                                                                                                    TAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAA                                                 <  SRR19973062.2536865/150‑1 (MQ=60)
                                                                                                     AATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAAT                                                >  SRR19973062.3372572/1‑150 (MQ=60)
                                                                                                     AATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAAT                                                <  SRR19973062.4051840/150‑1 (MQ=60)
                                                                                                     AATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAAT                                                >  SRR19973062.3373014/1‑150 (MQ=60)
                                                                                                       TCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATAC                                              <  SRR19973062.1514369/150‑1 (MQ=60)
                                                                                                        CAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACC                                             <  SRR19973062.108420/150‑1 (MQ=60)
                                                                                                        CAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACC                                             <  SRR19973062.2807590/150‑1 (MQ=60)
                                                                                                        AAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACC                                             <  SRR19973062.1500440/150‑1 (MQ=60)
                                                                                                         AAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCG                                            <  SRR19973062.4720808/150‑1 (MQ=60)
                                                                                                           ATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCG                                          <  SRR19973062.700477/150‑1 (MQ=60)
                                                                                                           ATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCG                                          <  SRR19973062.3251098/150‑1 (MQ=60)
                                                                                                             CACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTG                                        <  SRR19973062.2612108/150‑1 (MQ=60)
                                                                                                                GCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACA                                     <  SRR19973062.4021047/150‑1 (MQ=60)
                                                                                                                GCATACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGAAGTGACGAAACGAAATACCGCGTGACA                                     >  SRR19973062.2140201/1‑150 (MQ=60)
                                                                                                                   TACGCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCC                                  >  SRR19973062.1729037/1‑150 (MQ=60)
                                                                                                                    ACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCA                                 >  SRR19973062.43937/1‑150 (MQ=60)
                                                                                                                    ACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCA                                 <  SRR19973062.3759769/150‑1 (MQ=60)
                                                                                                                     CGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAT                                <  SRR19973062.2816377/150‑1 (MQ=60)
                                                                                                                     CGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAT                                <  SRR19973062.2816361/150‑1 (MQ=60)
                                                                                                                      GCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATC                               <  SRR19973062.4796504/150‑1 (MQ=60)
                                                                                                                      GCGCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATC                               <  SRR19973062.2131224/150‑1 (MQ=60)
                                                                                                                        GCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAC                             >  SRR19973062.2947559/1‑150 (MQ=60)
                                                                                                                        GCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAC                             >  SRR19973062.1237750/1‑150 (MQ=60)
                                                                                                                        GCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAC                             >  SRR19973062.2479761/1‑150 (MQ=60)
                                                                                                                        GCTCTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAC                             >  SRR19973062.2178820/1‑150 (MQ=60)
                                                                                                                         CTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGACACGAAATACCGCGTGACAACCATCACG                            >  SRR19973062.4865020/1‑150 (MQ=60)
                                                                                                                          TCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGC                           >  SRR19973062.337328/1‑150 (MQ=60)
                                                                                                                          TCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGC                           >  SRR19973062.337335/1‑150 (MQ=60)
                                                                                                                           CTCGACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCG                          <  SRR19973062.1951685/150‑1 (MQ=60)
                                                                                                                               ACTACAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAG                      <  SRR19973062.4976156/150‑1 (MQ=60)
                                                                                                                                   CAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACAT                  <  SRR19973062.4703515/150‑1 (MQ=60)
                                                                                                                                   CAGTATGCATCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACAT                  <  SRR19973062.4703479/150‑1 (MQ=60)
                                                                                                                                   CAGTATGCATCTTATATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACAT                  >  SRR19973062.1160386/1‑150 (MQ=60)
                                                                                                                                         GCATATTTTATGCCAAATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATT            <  SRR19973062.307425/150‑1 (MQ=60)
                                                                                                                                          CATATTTTATAACACATTTTATGTGGGGTCATTCCCTGATATTACTGGCACTATTTATTCAAAACTCTGACGAAAAACAGGATTACGTCAGTTTTGACGTGACGATACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTT           <  SRR19973062.2015935/150‑1 (MQ=60)
                                                                                                                                            TCTTTTATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTT         <  SRR19973062.890300/150‑1 (MQ=60)
                                                                                                                                                 ttgGCCACATTTTCTGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTGGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCG    <  SRR19973062.4501930/147‑1 (MQ=60)
                                                                                                                                                 TATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCG    <  SRR19973062.3957439/150‑1 (MQ=60)
                                                                                                                                                 TATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCG    <  SRR19973062.2239813/150‑1 (MQ=60)
                                                                                                                                                 TATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCG    <  SRR19973062.2243573/150‑1 (MQ=60)
                                                                                                                                                  ATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGA   >  SRR19973062.3914721/1‑150 (MQ=60)
                                                                                                                                                  ATACCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGA   >  SRR19973062.1194272/1‑150 (MQ=60)
                                                                                                                                                   TGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGAC  >  SRR19973062.1803092/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGAC  >  CP009273/4289099‑4289395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑