Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F56 I0 R1 543 285.0 9366284 95.7% 8963533 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 395,611 C→A 7.9% D257Y (GAC→TAC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273395,6110CA 7.9% 1283.3 / 63.0 342D257Y (GAC→TAC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (132/181);  new base A (11/16);  total (143/199)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 345 total aligned reads displayed.

GTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACG  >  CP009273/395463‑395759
                                                                                                                                                    |                                                                                                                                                    
 ttAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCg                                                                                                                                                     <  2:1309785/149‑1 (MQ=255)
    aaaaCACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAg                                                                                                                                                  <  1:2764484/149‑1 (MQ=255)
     aaaCACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAgg                                                                                                                                                 <  1:1383759/149‑1 (MQ=255)
         acGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATa                                                                                                                                             >  2:4142230/1‑149 (MQ=255)
            tCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGaa                                                                                                                                          <  2:3781527/149‑1 (MQ=255)
            tCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGaa                                                                                                                                          <  2:3781593/149‑1 (MQ=255)
                 acGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGc                                                                                                                                     >  2:2567505/1‑149 (MQ=255)
                 acGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGc                                                                                                                                     >  2:4673188/1‑149 (MQ=255)
                   gcgcCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTg                                                                                                                                   >  1:874532/1‑149 (MQ=255)
                    cgcCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTgg                                                                                                                                  <  2:1665178/149‑1 (MQ=255)
                       cATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGa                                                                                                                               >  2:87922/1‑149 (MQ=255)
                       cATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGa                                                                                                                               <  2:2982155/149‑1 (MQ=255)
                        aTGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAg                                                                                                                              >  2:487456/1‑149 (MQ=255)
                         tGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGt                                                                                                                             >  2:3873874/1‑149 (MQ=255)
                         tGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGt                                                                                                                             <  1:3517359/149‑1 (MQ=255)
                           ccTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTAc                                                                                                                           <  1:137695/149‑1 (MQ=255)
                           ccTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTAc                                                                                                                           <  2:3935860/149‑1 (MQ=255)
                             tGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGa                                                                                                                         <  1:687238/149‑1 (MQ=255)
                             tGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGa                                                                                                                         <  1:3663958/149‑1 (MQ=255)
                              gcgcATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGAt                                                                                                                        >  1:3148365/1‑149 (MQ=255)
                                gcATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGATATCGCTGGTGAGTACGAtct                                                                                                                      >  1:739702/1‑149 (MQ=255)
                                 cATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGAtctc                                                                                                                     >  1:4204206/1‑149 (MQ=255)
                                  aTCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGAtctct                                                                                                                    >  1:4022653/1‑149 (MQ=255)
                                    cccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCTCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTcc                                                                                                                  <  2:2382453/149‑1 (MQ=255)
                                     ccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCca                                                                                                                 >  2:335798/1‑149 (MQ=255)
                                       aaCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcaca                                                                                                               >  2:1448021/1‑149 (MQ=255)
                                       aaCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcaca                                                                                                               <  1:1955851/149‑1 (MQ=255)
                                       aaCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATCTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcaca                                                                                                               >  2:803627/1‑149 (MQ=255)
                                        aCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAg                                                                                                              <  1:817323/149‑1 (MQ=255)
                                          gTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGt                                                                                                            >  1:1918298/1‑149 (MQ=255)
                                          gTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGt                                                                                                            >  1:4252675/1‑149 (MQ=255)
                                           tttGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTg                                                                                                           >  1:3015861/1‑149 (MQ=255)
                                              gATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTg                                                                                                        <  2:578527/149‑1 (MQ=255)
                                                tAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGc                                                                                                      >  1:3538444/1‑149 (MQ=255)
                                                  aGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCtt                                                                                                    >  1:3733400/1‑149 (MQ=255)
                                                   gCCTGAACGGCAATCGCCCGGATCTTATCGTCGATTTCTGGCGCAAGGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTg                                                                                                   <  2:2205558/149‑1 (MQ=255)
                                                    ccTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGc                                                                                                  >  1:4508755/1‑149 (MQ=255)
                                                     cTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCg                                                                                                 <  1:2121880/149‑1 (MQ=255)
                                                     cTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCg                                                                                                 <  1:2423314/149‑1 (MQ=255)
                                                      tGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCgg                                                                                                >  1:3084099/1‑149 (MQ=255)
                                                      tGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCgg                                                                                                >  2:3384890/1‑149 (MQ=255)
                                                       gAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGa                                                                                               <  2:3075839/149‑1 (MQ=255)
                                                       gAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGa                                                                                               <  2:1144003/149‑1 (MQ=255)
                                                        aaCGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGAt                                                                                              <  2:370117/149‑1 (MQ=255)
                                                          cGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTg                                                                                            >  1:3373506/1‑149 (MQ=255)
                                                          cGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTg                                                                                            >  1:3373422/1‑149 (MQ=255)
                                                               aTCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGtt                                                                                       <  1:387947/149‑1 (MQ=255)
                                                                tCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTg                                                                                      <  1:2544842/149‑1 (MQ=255)
                                                                   cccGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGccc                                                                                   <  1:4293401/149‑1 (MQ=255)
                                                                     cGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAg                                                                                 >  2:1602414/1‑149 (MQ=255)
                                                                       gATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGaa                                                                               <  1:30503/149‑1 (MQ=255)
                                                                         tCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACt                                                                             >  1:3947487/1‑149 (MQ=255)
                                                                           ttATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgc                                                                           <  2:1425961/149‑1 (MQ=255)
                                                                            tATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcg                                                                          >  2:1776139/1‑149 (MQ=255)
                                                                            tATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcg                                                                          >  2:2491627/1‑149 (MQ=255)
                                                                             aTCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcgc                                                                         >  1:2149679/1‑149 (MQ=255)
                                                                             aTCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcgc                                                                         <  2:3304707/149‑1 (MQ=255)
                                                                              tCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCa                                                                        <  2:1873236/149‑1 (MQ=255)
                                                                                gTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCAtt                                                                      <  2:2034069/149‑1 (MQ=255)
                                                                                    aTTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAt                                                                  <  2:2332391/149‑1 (MQ=255)
                                                                                     tttCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAtc                                                                 <  1:4673188/149‑1 (MQ=255)
                                                                                     tttCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAtc                                                                 >  2:3055415/1‑149 (MQ=255)
                                                                                         tGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                             <  1:1606882/149‑1 (MQ=255)
                                                                                              cAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCt                                                        <  1:2800093/149‑1 (MQ=255)
                                                                                               aaTGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                       >  2:719392/1‑149 (MQ=255)
                                                                                                aTGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                      >  1:458880/1‑149 (MQ=255)
                                                                                                aTGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                      >  1:309586/1‑149 (MQ=255)
                                                                                                 tGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                     <  2:1746230/149‑1 (MQ=255)
                                                                                                 tGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                     <  1:2338845/149‑1 (MQ=255)
                                                                                                  ggCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGa                                                    >  1:2247623/1‑149 (MQ=255)
                                                                                                     tGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATcc                                                 >  2:3900687/1‑149 (MQ=255)
                                                                                                       ccGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                               <  1:106751/149‑1 (MQ=255)
                                                                                                       ccGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                               >  2:1083772/1‑149 (MQ=255)
                                                                                                        cGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAATTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                              <  1:4075395/149‑1 (MQ=255)
                                                                                                         ggAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                             >  2:2711499/1‑149 (MQ=255)
                                                                                                         ggAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                             >  1:1388352/1‑149 (MQ=255)
                                                                                                             cTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCTCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                         >  1:3039212/1‑149 (MQ=255)
                                                                                                                    tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                  <  2:147368/149‑1 (MQ=255)
                                                                                                                     ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                 <  2:3074323/149‑1 (MQ=255)
                                                                                                                       cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                               <  2:2866769/149‑1 (MQ=255)
                                                                                                                       cgcgCCATCTTCGTCAATGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                               <  2:4313745/149‑1 (MQ=255)
                                                                                                                          gcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                            <  2:1559972/149‑1 (MQ=255)
                                                                                                                          gcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                            <  1:4427346/149‑1 (MQ=255)
                                                                                                                          gcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                            <  1:1269095/149‑1 (MQ=255)
                                                                                                                           ccATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                           >  1:1195138/1‑149 (MQ=255)
                                                                                                                            cATCTTCGTCAATGTACTTGGTGTTGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                          <  2:4021861/149‑1 (MQ=255)
                                                                                                                                ttCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                      <  2:2804892/149‑1 (MQ=255)
                                                                                                                                  cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                    <  2:3796622/149‑1 (MQ=255)
                                                                                                                                  cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                    <  2:4593736/149‑1 (MQ=255)
                                                                                                                                  cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                    <  2:435222/149‑1 (MQ=255)
                                                                                                                                     cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                 >  1:1116695/1‑149 (MQ=255)
                                                                                                                                      aaTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                >  2:1957328/1‑149 (MQ=255)
                                                                                                                                      aaTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                >  1:1527508/1‑149 (MQ=255)
                                                                                                                                      aaTGTACTTGGTGTAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                >  2:2182012/1‑149 (MQ=255)
                                                                                                                                       aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa               <  1:3134648/149‑1 (MQ=255)
                                                                                                                                       aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa               >  1:2004006/1‑149 (MQ=255)
                                                                                                                                           aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc           <  2:4388847/149‑1 (MQ=255)
                                                                                                                                             ttGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAg         >  2:4453697/1‑149 (MQ=255)
                                                                                                                                               ggTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt       <  2:317034/149‑1 (MQ=255)
                                                                                                                                                gtgtAGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc      >  2:1193638/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACG  >  CP009273/395463‑395759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A