Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,380,078 C→T 100% S247F (TCT→TTT)  yfbL → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,380,0780CT99.2% 795.7 / ‑2.4 241S247F (TCT→TTT) yfbLputative peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (99/140);  total (99/142)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

TATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTT  >  CP009273/2379933‑2380222
                                                                                                                                                 |                                                                                                                                                
 aTTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAg                                                                                                                                              >  1:663121/1‑149 (MQ=255)
 aTTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAg                                                                                                                                              >  1:1972540/1‑149 (MQ=255)
   tACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGa                                                                                                                                            <  2:3680320/149‑1 (MQ=255)
   tACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGa                                                                                                                                            <  2:1460677/149‑1 (MQ=255)
    aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAt                                                                                                                                           <  2:1375151/149‑1 (MQ=255)
     cGACTCGGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                          <  2:1865769/149‑1 (MQ=255)
         tctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATc                                                                                                                                      <  1:3677296/149‑1 (MQ=255)
          ctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCt                                                                                                                                     <  2:1850518/149‑1 (MQ=255)
           tGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTg                                                                                                                                    >  1:1097450/1‑149 (MQ=255)
            gcgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGACAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGt                                                                                                                                   <  1:2020669/149‑1 (MQ=255)
                cTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTAt                                                                                                                               <  1:3081343/149‑1 (MQ=255)
                  ggAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTc                                                                                                                             >  1:4042943/1‑149 (MQ=255)
                  ggAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTc                                                                                                                             <  1:4005941/149‑1 (MQ=255)
                  ggAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTc                                                                                                                             >  1:3771830/1‑149 (MQ=255)
                    aaGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTa                                                                                                                           <  2:1476166/149‑1 (MQ=255)
                      gCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATg                                                                                                                         <  1:3871425/149‑1 (MQ=255)
                      gCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATg                                                                                                                         <  1:3350509/149‑1 (MQ=255)
                         aGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAt                                                                                                                      >  2:1578148/1‑149 (MQ=255)
                          gAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATa                                                                                                                     <  2:1762305/149‑1 (MQ=255)
                          gAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATa                                                                                                                     >  2:2975694/1‑149 (MQ=255)
                            aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcc                                                                                                                   >  1:845966/1‑149 (MQ=255)
                            aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcc                                                                                                                   >  1:929741/1‑149 (MQ=255)
                            aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcc                                                                                                                   >  1:3027426/1‑149 (MQ=255)
                             ttacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAccc                                                                                                                  >  1:104524/1‑149 (MQ=255)
                              tacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                 >  2:3889914/1‑149 (MQ=255)
                              tacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                 >  2:232152/1‑149 (MQ=255)
                               acccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                >  1:2269317/1‑149 (MQ=255)
                               acccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                <  1:3110190/149‑1 (MQ=255)
                                cccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAg                                                                                                               <  2:3101729/149‑1 (MQ=255)
                                ccctgacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAg                                                                                                               <  1:1808326/149‑1 (MQ=255)
                                  cttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAggg                                                                                                             >  1:2986281/1‑149 (MQ=255)
                                    tacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGtt                                                                                                           <  1:500697/149‑1 (MQ=255)
                                     acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGttt                                                                                                          <  1:2604250/149‑1 (MQ=255)
                                          gCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTcc                                                                                                     >  2:609747/1‑149 (MQ=255)
                                             aTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCgg                                                                                                  >  1:1571077/1‑149 (MQ=255)
                                                    ggCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAt                                                                                           >  1:3958955/1‑149 (MQ=255)
                                                     gCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAtt                                                                                          >  1:3372061/1‑149 (MQ=255)
                                                         tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                      <  1:3038953/149‑1 (MQ=255)
                                                         tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                      <  2:394633/149‑1 (MQ=255)
                                                         tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                      <  2:394550/149‑1 (MQ=255)
                                                         tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                      <  1:1338848/149‑1 (MQ=255)
                                                         tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                      <  1:2749982/149‑1 (MQ=255)
                                                          aTCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTctct                                                                                     >  2:2172866/1‑149 (MQ=255)
                                                             ccGATCGGGGAGACTTGATTGCCGTGGTCGGCAGAAGACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGac                                                                                  <  2:2847537/149‑1 (MQ=255)
                                                               gATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacca                                                                                >  2:2519776/1‑149 (MQ=255)
                                                                aTCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGaccac                                                                               >  1:3396216/1‑149 (MQ=255)
                                                                aTCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGaccac                                                                               >  1:2876620/1‑149 (MQ=255)
                                                                 tCGGGGAGACTTTATTGCCGTGGTCGACAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGaccacc                                                                              <  2:1264528/149‑1 (MQ=255)
                                                                    gggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGa                                                                           >  1:3166416/1‑149 (MQ=255)
                                                                    gggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGa                                                                           >  1:793588/1‑149 (MQ=255)
                                                                    gggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGa                                                                           >  1:1114190/1‑149 (MQ=255)
                                                                      gagaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAat                                                                         <  2:3211088/149‑1 (MQ=255)
                                                                        gaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAatta                                                                       <  1:3463495/149‑1 (MQ=255)
                                                                        gaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAatta                                                                       <  2:2686219/149‑1 (MQ=255)
                                                                          cTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCGCTGACCACCTGAattatt                                                                     <  2:2142578/149‑1 (MQ=255)
                                                                             tATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGc                                                                  <  1:251126/149‑1 (MQ=255)
                                                                             tATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGc                                                                  <  2:2418305/149‑1 (MQ=255)
                                                                                tGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAac                                                               <  2:112760/149‑1 (MQ=255)
                                                                                  ccGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAacac                                                             <  1:2772043/149‑1 (MQ=255)
                                                                                    gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTTTTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGa                                                           <  1:3202183/149‑1 (MQ=255)
                                                                                      ggTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGata                                                         <  1:1950068/149‑1 (MQ=255)
                                                                                      ggTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGata                                                         >  1:4098701/1‑149 (MQ=255)
                                                                                          ggCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTcc                                                     <  2:3777085/149‑1 (MQ=255)
                                                                                           gCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTccc                                                    <  1:3387520/149‑1 (MQ=255)
                                                                                             aGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGc                                                  <  2:1872744/149‑1 (MQ=255)
                                                                                              gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                 >  2:2623989/1‑149 (MQ=255)
                                                                                              gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                 <  2:2761526/149‑1 (MQ=255)
                                                                                              gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                 <  2:3927569/149‑1 (MQ=255)
                                                                                              gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                 >  1:1626946/1‑149 (MQ=255)
                                                                                                  aCAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATaa                                             <  2:1563807/149‑1 (MQ=255)
                                                                                                  aCAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATaa                                             <  2:360995/149‑1 (MQ=255)
                                                                                                  aCAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATaa                                             <  2:1220125/149‑1 (MQ=255)
                                                                                                   cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAAt                                            <  2:693145/149‑1 (MQ=255)
                                                                                                   cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAAt                                            <  2:3367862/149‑1 (MQ=255)
                                                                                                   cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAAt                                            <  2:2877506/149‑1 (MQ=255)
                                                                                                   cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAAt                                            <  2:904873/149‑1 (MQ=255)
                                                                                                     ggATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGa                                          <  2:854097/149‑1 (MQ=255)
                                                                                                     ggATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGa                                          <  1:2730729/149‑1 (MQ=255)
                                                                                                      gATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAt                                         <  1:599150/149‑1 (MQ=255)
                                                                                                      gATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAt                                         >  1:1419085/1‑149 (MQ=255)
                                                                                                        tatCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTa                                       >  1:243947/1‑149 (MQ=255)
                                                                                                            aaCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGa                                   <  2:1972540/149‑1 (MQ=255)
                                                                                                             aCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGac                                  >  1:2951042/1‑149 (MQ=255)
                                                                                                             aCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGac                                  <  2:549314/149‑1 (MQ=255)
                                                                                                              cGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGaca                                 <  1:2677206/149‑1 (MQ=255)
                                                                                                               gCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGacac                                >  2:3424563/1‑149 (MQ=255)
                                                                                                                   ttCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCt                            >  1:1996078/1‑149 (MQ=255)
                                                                                                                       tCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCttttt                        >  1:906832/1‑149 (MQ=255)
                                                                                                                              aaaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaa                 >  1:255140/1‑149 (MQ=255)
                                                                                                                                 gCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaataa              >  2:442051/1‑149 (MQ=255)
                                                                                                                                     cATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACaa          <  2:4098701/149‑1 (MQ=255)
                                                                                                                                      aTTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACAACGCTTTTTATCGTAATAAACAAt         <  2:2528237/149‑1 (MQ=255)
                                                                                                                                       ttgttgTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATa        >  2:2123949/1‑149 (MQ=255)
                                                                                                                                       ttgttgTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATa        <  1:3197910/149‑1 (MQ=255)
                                                                                                                                          ttgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCa     <  2:2950612/149‑1 (MQ=255)
                                                                                                                                          ttgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCa     >  2:765869/1‑149 (MQ=255)
                                                                                                                                          ttgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCa     >  2:2827690/1‑149 (MQ=255)
                                                                                                                                           tgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCAc    <  2:754492/149‑1 (MQ=255)
                                                                                                                                            gtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACt   >  1:187476/1‑149 (MQ=255)
                                                                                                                                             tCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACtt  >  2:2594391/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTT  >  CP009273/2379933‑2380222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCC  >  CP009273/2379930‑2380226
                                                                                                                                                    |                                                                                                                                                    
GGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTT                                                                                                                                                     <  SRR19973068.1745972/150‑1 (MQ=60)
GGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTT                                                                                                                                                     >  SRR19973068.3409606/1‑150 (MQ=60)
 GCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTC                                                                                                                                                    <  SRR19973068.3258822/150‑1 (MQ=60)
  CTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCA                                                                                                                                                   >  SRR19973068.3077923/1‑150 (MQ=60)
  CTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCA                                                                                                                                                   >  SRR19973068.2027053/1‑150 (MQ=60)
   TATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAG                                                                                                                                                  >  SRR19973068.733764/1‑150 (MQ=60)
   TATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAG                                                                                                                                                  >  SRR19973068.1985603/1‑150 (MQ=60)
   TATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAG                                                                                                                                                  >  SRR19973068.667377/1‑150 (MQ=60)
            TCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCT                                                                                                                                         <  SRR19973068.3699505/150‑1 (MQ=60)
             CTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTG                                                                                                                                        >  SRR19973068.1104759/1‑150 (MQ=60)
             CTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTG                                                                                                                                        >  SRR19973068.1104806/1‑150 (MQ=60)
             CTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTG                                                                                                                                        <  SRR19973068.2661950/150‑1 (MQ=60)
              TGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGT                                                                                                                                       >  SRR19973068.3118192/1‑150 (MQ=60)
              TGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGT                                                                                                                                       >  SRR19973068.500689/1‑150 (MQ=60)
               GCGCCTGGAAGCCAGAATTACCCTTACCCTGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCTGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTT                                                                                                                                      <  SRR19973068.934417/150‑1 (MQ=60)
               GCGCCTGGAAGCCAGAATTACCCTTACCCTGCAAGGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTT                                                                                                                                      <  SRR19973068.3005267/150‑1 (MQ=60)
               GCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGACAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTT                                                                                                                                      <  SRR19973068.2033929/150‑1 (MQ=60)
                 GCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTA                                                                                                                                    <  SRR19973068.2345069/150‑1 (MQ=60)
                   CTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATT                                                                                                                                  <  SRR19973068.3441445/150‑1 (MQ=60)
                   CTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATT                                                                                                                                  <  SRR19973068.3100124/150‑1 (MQ=60)
                   CTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATT                                                                                                                                  <  SRR19973068.2688839/150‑1 (MQ=60)
                    TGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTC                                                                                                                                 >  SRR19973068.4067298/1‑150 (MQ=60)
                    TGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTC                                                                                                                                 >  SRR19973068.3794335/1‑150 (MQ=60)
                    TGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTC                                                                                                                                 <  SRR19973068.3200097/150‑1 (MQ=60)
                     GGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCT                                                                                                                                <  SRR19973068.4030144/150‑1 (MQ=60)
                     GGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCT                                                                                                                                >  SRR19973068.3388327/1‑150 (MQ=60)
                     GGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCT                                                                                                                                >  SRR19973068.910980/1‑150 (MQ=60)
                      GAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTA                                                                                                                               <  SRR19973068.3220909/150‑1 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCGGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            <  SRR19973068.3895656/150‑1 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            >  SRR19973068.3778870/1‑150 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            >  SRR19973068.2601272/1‑150 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            <  SRR19973068.3894799/150‑1 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            <  SRR19973068.3369324/150‑1 (MQ=60)
                         GCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGA                                                                                                                            <  SRR19973068.3370918/150‑1 (MQ=60)
                           CAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAT                                                                                                                          <  SRR19973068.2750333/150‑1 (MQ=60)
                           CAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAT                                                                                                                          <  SRR19973068.3939180/150‑1 (MQ=60)
                            AGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATA                                                                                                                         >  SRR19973068.1885136/1‑150 (MQ=60)
                            AGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATA                                                                                                                         >  SRR19973068.214082/1‑150 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       <  SRR19973068.1359992/150‑1 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       >  SRR19973068.851505/1‑150 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       >  SRR19973068.2544309/1‑150 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       >  SRR19973068.3046037/1‑150 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       <  SRR19973068.339646/150‑1 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       >  SRR19973068.935980/1‑150 (MQ=60)
                              AATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACC                                                                                                                       >  SRR19973068.851720/1‑150 (MQ=60)
                               ATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCC                                                                                                                      >  SRR19973068.105554/1‑150 (MQ=60)
                                TTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCC                                                                                                                     <  SRR19973068.404336/150‑1 (MQ=60)
                                TTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCC                                                                                                                     <  SRR19973068.404423/150‑1 (MQ=60)
                                 TACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCA                                                                                                                    <  SRR19973068.72672/150‑1 (MQ=60)
                                 TACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCA                                                                                                                    >  SRR19973068.2284038/1‑150 (MQ=60)
                                 TACCCTTACCCGGAAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCATAATACTGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCA                                                                                                                    <  SRR19973068.1524500/150‑1 (MQ=60)
                                  ACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAG                                                                                                                   <  SRR19973068.3129172/150‑1 (MQ=60)
                                   CCCTTACCCGGCAAGGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGG                                                                                                                  <  SRR19973068.1820211/150‑1 (MQ=60)
                                   CCCTGACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGG                                                                                                                  <  SRR19973068.1820192/150‑1 (MQ=60)
                                   CCCTGACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGG                                                                                                                  <  SRR19973068.3809244/150‑1 (MQ=60)
                                    CCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGG                                                                                                                 <  SRR19973068.2817088/150‑1 (MQ=60)
                                    CCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGG                                                                                                                 >  SRR19973068.3004755/1‑150 (MQ=60)
                                      TTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTCTGAATACCCCAGGGTT                                                                                                               >  SRR19973068.2170973/1‑150 (MQ=60)
                                       TACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTT                                                                                                              <  SRR19973068.504195/150‑1 (MQ=60)
                                        ACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTA                                                                                                             <  SRR19973068.2620679/150‑1 (MQ=60)
                                         CCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTAT                                                                                                            <  SRR19973068.1150663/150‑1 (MQ=60)
                                          CCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATT                                                                                                           <  SRR19973068.520081/150‑1 (MQ=60)
                                           CGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTC                                                                                                          <  SRR19973068.1076498/150‑1 (MQ=60)
                                               AATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGG                                                                                                      >  SRR19973068.1581408/1‑150 (MQ=60)
                                                      TGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAT                                                                                               >  SRR19973068.3982906/1‑150 (MQ=60)
                                                      TGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAT                                                                                               >  SRR19973068.1983925/1‑150 (MQ=60)
                                                       GGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATT                                                                                              >  SRR19973068.3392536/1‑150 (MQ=60)
                                                         CTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAgatcggaagagcacacgtctgaactccagtc                                                                                            >  SRR19973068.4018273/1‑119 (MQ=60)
                                                            TATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCT                                                                                         <  SRR19973068.3057603/150‑1 (MQ=60)
                                                            TATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCT                                                                                         <  SRR19973068.1347504/150‑1 (MQ=60)
                                                            TATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCT                                                                                         <  SRR19973068.2766974/150‑1 (MQ=60)
                                                            TATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCT                                                                                         <  SRR19973068.2766884/150‑1 (MQ=60)
                                                                  GATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCAC                                                                                   >  SRR19973068.2894532/1‑150 (MQ=60)
                                                                  GATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCAC                                                                                   >  SRR19973068.3416779/1‑150 (MQ=60)
                                                                  GATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCAC                                                                                   >  SRR19973068.3416776/1‑150 (MQ=60)
                                                                    TCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCT                                                                                 <  SRR19973068.2751463/150‑1 (MQ=60)
                                                                     CGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTG                                                                                >  SRR19973068.2880498/1‑150 (MQ=60)
                                                                      GGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGA                                                                               >  SRR19973068.1121636/1‑150 (MQ=60)
                                                                      GGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGA                                                                               >  SRR19973068.3301317/1‑150 (MQ=60)
                                                                      GGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGA                                                                               >  SRR19973068.1709290/1‑150 (MQ=60)
                                                                      GGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGA                                                                               >  SRR19973068.798983/1‑150 (MQ=60)
                                                                      GGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGA                                                                               >  SRR19973068.3185885/1‑150 (MQ=60)
                                                                         GAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATT                                                                            <  SRR19973068.1986796/150‑1 (MQ=60)
                                                                           GACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTAT                                                                          <  SRR19973068.3484466/150‑1 (MQ=60)
                                                                            ACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATT                                                                         >  SRR19973068.3652563/1‑150 (MQ=60)
                                                                            ACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATT                                                                         <  SRR19973068.1913248/150‑1 (MQ=60)
                                                                               TTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGC                                                                      >  SRR19973068.3058618/1‑150 (MQ=60)
                                                                                TATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCA                                                                     <  SRR19973068.252943/150‑1 (MQ=60)
                                                                                    GCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACAC                                                                 <  SRR19973068.2695241/150‑1 (MQ=60)
                                                                                     CCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACG                                                                <  SRR19973068.2789153/150‑1 (MQ=60)
                                                                                       GTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTTTTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGAT                                                              <  SRR19973068.3221872/150‑1 (MQ=60)
                                                                                        TGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATA                                                             >  SRR19973068.4123258/1‑150 (MQ=60)
                                                                                        TGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATA                                                             >  SRR19973068.4123406/1‑150 (MQ=60)
                                                                                         GGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATAT                                                            <  SRR19973068.1963011/150‑1 (MQ=60)
                                                                                         GGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATAT                                                            <  SRR19973068.1963270/150‑1 (MQ=60)
                                                                                            CGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCC                                                         >  SRR19973068.86472/1‑150 (MQ=60)
                                                                                             GGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTCTTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCC                                                        >  SRR19973068.352257/1‑150 (MQ=60)
                                                                                              GCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCG                                                       <  SRR19973068.3408062/150‑1 (MQ=60)
                                                                                                AGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCC                                                     >  SRR19973068.1637562/1‑150 (MQ=60)
                                                                                                 GAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCA                                                    >  SRR19973068.163061/1‑150 (MQ=60)
                                                                                                 GAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCA                                                    <  SRR19973068.2504772/150‑1 (MQ=60)
                                                                                                        GGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAT                                             >  SRR19973068.1428299/1‑150 (MQ=60)
                                                                                                        GGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAT                                             >  SRR19973068.2508465/1‑150 (MQ=60)
                                                                                                        GGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAT                                             <  SRR19973068.2747573/150‑1 (MQ=60)
                                                                                                         GATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATT                                            <  SRR19973068.603187/150‑1 (MQ=60)
                                                                                                          ATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTA                                           >  SRR19973068.3051318/1‑150 (MQ=60)
                                                                                                          ATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTA                                           >  SRR19973068.245730/1‑150 (MQ=60)
                                                                                                               AACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGAC                                      >  SRR19973068.2969350/1‑150 (MQ=60)
                                                                                                               AACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGAC                                      >  SRR19973068.2968919/1‑150 (MQ=60)
                                                                                                                 CGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACAC                                    <  SRR19973068.2693897/150‑1 (MQ=60)
                                                                                                                  GCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACC                                   >  SRR19973068.3182476/1‑150 (MQ=60)
                                                                                                                     GTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCT                                >  SRR19973068.2009233/1‑150 (MQ=60)
                                                                                                                      TTCGTCAGTTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCATGGTTTATTCCCGGTATTGATTTCTCTGAACAACTGAATTATTGGCAACAAGATATTCCCGCCATAATGATTACTGACACCTCTT                               <  SRR19973068.1130042/150‑1 (MQ=60)
                                                                                                                      TTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTT                               <  SRR19973068.3194863/150‑1 (MQ=60)
                                                                                                                         GTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTT                            >  SRR19973068.912949/1‑150 (MQ=60)
                                                                                                                         GTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTT                            >  SRR19973068.3003559/1‑150 (MQ=60)
                                                                                                                           CAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTAT                          <  SRR19973068.654877/150‑1 (MQ=60)
                                                                                                                            AGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATC                         >  SRR19973068.4089590/1‑150 (MQ=60)
                                                                                                                                AAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAA                     >  SRR19973068.1569293/1‑150 (MQ=60)
                                                                                                                                AAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAA                     >  SRR19973068.256973/1‑150 (MQ=60)
                                                                                                                                 AAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAAT                    <  SRR19973068.3409875/150‑1 (MQ=60)
                                                                                                                                   AGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAA                  >  SRR19973068.3030341/1‑150 (MQ=60)
                                                                                                                                      GGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACA               <  SRR19973068.997918/150‑1 (MQ=60)
                                                                                                                                          TTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATAC           <  SRR19973068.3217583/150‑1 (MQ=60)
                                                                                                                                              TGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACT       >  SRR19973068.188955/1‑150 (MQ=60)
                                                                                                                                                  ATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCC   <  SRR19973068.3085136/150‑1 (MQ=60)
                                                                                                                                                   TTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCC  >  SRR19973068.1369120/1‑150 (MQ=60)
                                                                                                                                                   TTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCC  >  SRR19973068.1369109/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCC  >  CP009273/2379930‑2380226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑