Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,669,978 C→A 100% A69A (GCG→GCT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,669,9780CA96.2% 633.5 / 14.3 213A69A (GCG→GCT) yphDinner membrane putative ABC superfamily sugar transporter permease
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (98/107);  total (102/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

CCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGAC  >  CP009273/2669841‑2670123
                                                                                                                                         |                                                                                                                                                 
 cTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCa                                                                                                                                       <  1:1224509/149‑1 (MQ=255)
  tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCaa                                                                                                                                      <  1:3273651/149‑1 (MQ=255)
    aCAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATc                                                                                                                                    <  2:3334186/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAACTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCCGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:1812264/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:3844899/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:1812469/149‑1 (MQ=255)
         aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                               >  2:3614780/1‑149 (MQ=255)
         aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                               >  2:1278686/1‑149 (MQ=255)
          acacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                              >  2:3418049/1‑149 (MQ=255)
           cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtgg                                                                                                                             <  2:2614502/149‑1 (MQ=255)
             ccAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtggtg                                                                                                                           >  1:3955995/1‑149 (MQ=255)
              cagcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAAGTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtgg                                                                                                                          >  2:2401695/1‑149 (MQ=255)
               agcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTggc                                                                                                                         >  1:2806001/1‑149 (MQ=255)
                   ggCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                                                                     >  2:1926545/1‑149 (MQ=255)
                   ggCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                                                                     <  2:2576076/149‑1 (MQ=255)
                    gCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCAt                                                                                                                    <  2:3975507/149‑1 (MQ=255)
                      aCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg                                                                                                                  >  1:2120170/1‑149 (MQ=255)
                                 aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                       >  1:1114450/1‑149 (MQ=255)
                                 aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                       >  1:3520801/1‑149 (MQ=255)
                                  gCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTc                                                                                                      >  1:1707499/1‑149 (MQ=255)
                                    ggAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                    >  1:3451586/1‑149 (MQ=255)
                                     gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                   >  2:3883138/1‑149 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  1:3418049/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  2:3945030/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  1:3702854/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  2:2797595/149‑1 (MQ=255)
                                        cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTgg                                                                                                <  1:3905609/149‑1 (MQ=255)
                                         ttCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                               >  2:1595327/1‑149 (MQ=255)
                                             aaTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAtt                                                                                           >  1:3747204/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATCATCATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  2:11651/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  1:2043759/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  2:11538/1‑149 (MQ=255)
                                                tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                        <  2:3016282/149‑1 (MQ=255)
                                                tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                        <  1:537259/149‑1 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    >  1:1084925/1‑149 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    <  2:3173115/149‑1 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    <  2:3382145/149‑1 (MQ=255)
                                                     caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgaga                                                                                   >  1:3664805/1‑149 (MQ=255)
                                                     caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgaga                                                                                   <  1:2380207/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:3120603/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:3120493/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:1214054/149‑1 (MQ=255)
                                                       aaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATa                                                                                 >  1:2236586/1‑149 (MQ=255)
                                                        aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                >  2:2461042/1‑149 (MQ=255)
                                                        aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                <  2:1707499/149‑1 (MQ=255)
                                                         aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                               <  1:3780185/149‑1 (MQ=255)
                                                         aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                               >  1:252964/1‑149 (MQ=255)
                                                          aTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAg                                                                              >  1:1414022/1‑149 (MQ=255)
                                                           tGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                             <  1:802755/149‑1 (MQ=255)
                                                            gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGcc                                                                            <  1:3376961/149‑1 (MQ=255)
                                                               aGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                                                                         >  1:742031/1‑149 (MQ=255)
                                                                      ccGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTc                                                                  >  2:2903743/1‑149 (MQ=255)
                                                                        gagaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAg                                                                <  2:894870/149‑1 (MQ=255)
                                                                         agaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAgg                                                               <  2:2221810/149‑1 (MQ=255)
                                                                           aCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGga                                                             >  1:3831045/1‑149 (MQ=255)
                                                                             aaaaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGgaga                                                           <  1:1623234/149‑1 (MQ=255)
                                                                               aaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaag                                                         >  2:3276166/1‑149 (MQ=255)
                                                                                  ccaccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGAcc                                                      >  2:2560941/1‑149 (MQ=255)
                                                                                  ccaccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGAcc                                                      >  2:3767416/1‑149 (MQ=255)
                                                                                   caccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCa                                                     >  2:1648656/1‑149 (MQ=255)
                                                                                   caccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCa                                                     <  2:2188731/149‑1 (MQ=255)
                                                                                    accaTCGGCCCAACGCTGACATCAATTTCTCCGGAGATATTAATCAGCGTCATAGCCCAGGCGGCACTCCCACTGGTGGCGGCATCGCGCAGCACGTTCATCTTGTTATTAAATGAGATAAAGCCAGGCGCGTTATGGGAGAAGAGCAg                                                    <  2:1669171/149‑1 (MQ=255)
                                                                                    accaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAg                                                    <  2:653962/149‑1 (MQ=255)
                                                                                     ccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGa                                                   >  2:20276/1‑149 (MQ=255)
                                                                                      caTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGAt                                                  <  2:1761931/149‑1 (MQ=255)
                                                                                           gCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGa                                             >  1:3783267/1‑149 (MQ=255)
                                                                                            cccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGaa                                            <  1:778054/149‑1 (MQ=255)
                                                                                             ccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                           <  1:847942/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  1:443730/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  2:864864/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          >  2:2076202/1‑149 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  1:445430/149‑1 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATCCTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        <  2:2441503/149‑1 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:2321927/1‑149 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:1178876/1‑149 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:46559/1‑149 (MQ=255)
                                                                                                   cTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAAt                                     <  1:729522/149‑1 (MQ=255)
                                                                                                    tGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATc                                    <  1:1670325/149‑1 (MQ=255)
                                                                                                     gACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                   <  1:1557078/149‑1 (MQ=255)
                                                                                                     gACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                   <  2:3614085/149‑1 (MQ=255)
                                                                                                         tCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcaccac                               >  2:1051465/1‑149 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCACTGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             <  2:2964042/149‑1 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             >  2:4108494/1‑149 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             >  1:2579402/1‑149 (MQ=255)
                                                                                                            aTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAg                            >  2:1490996/1‑149 (MQ=255)
                                                                                                                 aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                       >  1:425902/1‑149 (MQ=255)
                                                                                                                 aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                       <  1:2398886/149‑1 (MQ=255)
                                                                                                                     gagaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                   <  2:3301158/149‑1 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  <  1:976278/149‑1 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  >  1:2581603/1‑149 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  >  1:4043359/1‑149 (MQ=255)
                                                                                                                        ataataATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc                >  1:2732672/1‑149 (MQ=255)
                                                                                                                           ataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCAtt             >  1:1047259/1‑149 (MQ=255)
                                                                                                                            taatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa            >  2:1182946/1‑149 (MQ=255)
                                                                                                                             aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGATAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa           <  2:3664805/149‑1 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCCATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          <  1:3891555/149‑1 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          >  1:2337289/1‑149 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          >  1:2739368/1‑149 (MQ=255)
                                                                                                                                 aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATg       >  1:3289545/1‑149 (MQ=255)
                                                                                                                                  gCGTCATAGCCCAGGCGGCTATCCCTATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCATTGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATgg      <  2:764299/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
CCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGAC  >  CP009273/2669841‑2670123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACTGAC  >  CP009273/2669829‑2670127
                                                                                                                                                     |                                                                                                                                                     
CCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATA                                                                                                                                                       >  SRR19973068.2203048/1‑150 (MQ=60)
 CCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAG                                                                                                                                                      <  SRR19973068.906175/150‑1 (MQ=60)
 CCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAG                                                                                                                                                      >  SRR19973068.2793672/1‑150 (MQ=60)
           GCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGC                                                                                                                                            >  SRR19973068.2714063/1‑150 (MQ=60)
            CCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCA                                                                                                                                           <  SRR19973068.1672325/150‑1 (MQ=60)
             CTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAA                                                                                                                                          <  SRR19973068.1232650/150‑1 (MQ=60)
              TAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAAT                                                                                                                                         <  SRR19973068.3293739/150‑1 (MQ=60)
              TAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAAT                                                                                                                                         >  SRR19973068.3852006/1‑150 (MQ=60)
              TAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAAT                                                                                                                                         <  SRR19973068.1670052/150‑1 (MQ=60)
                  AGCAACCCCAGCTGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCA                                                                                                                                     <  SRR19973068.1824273/150‑1 (MQ=60)
                  AGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAACTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCCGCGTCATAGCCCAGGCGGCAATCCCA                                                                                                                                     <  SRR19973068.1824167/150‑1 (MQ=60)
                  AGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCA                                                                                                                                     <  SRR19973068.1824375/150‑1 (MQ=60)
                  AGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCA                                                                                                                                     <  SRR19973068.3867510/150‑1 (MQ=60)
                  AGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCA                                                                                                                                     <  SRR19973068.3868102/150‑1 (MQ=60)
                   GCAACACCCGCAGGCACGTCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAA                                                                                                                                    <  SRR19973068.3469972/150‑1 (MQ=60)
                    CAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAT                                                                                                                                   <  SRR19973068.861649/150‑1 (MQ=60)
                     AACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATG                                                                                                                                  <  SRR19973068.430499/150‑1 (MQ=60)
                        ACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTG                                                                                                                               >  SRR19973068.3979930/1‑150 (MQ=60)
                          CAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGC                                                                                                                             >  SRR19973068.2823438/1‑150 (MQ=60)
                          CAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGC                                                                                                                             <  SRR19973068.646325/150‑1 (MQ=60)
                                 CACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCG                                                                                                                      >  SRR19973068.2134025/1‑150 (MQ=60)
                                 CACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCG                                                                                                                      >  SRR19973068.3402654/1‑150 (MQ=60)
                                     CCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCA                                                                                                                  >  SRR19973068.3896601/1‑150 (MQ=60)
                                      CACCGCCAGCGGAACTTCACATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAG                                                                                                                 <  SRR19973068.2361004/150‑1 (MQ=60)
                                         CGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCAC                                                                                                              <  SRR19973068.1096060/150‑1 (MQ=60)
                                            CAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTT                                                                                                           >  SRR19973068.1121897/1‑150 (MQ=60)
                                            CAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTT                                                                                                           >  SRR19973068.3541566/1‑150 (MQ=60)
                                            CAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTT                                                                                                           >  SRR19973068.3541660/1‑150 (MQ=60)
                                            CAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTT                                                                                                           >  SRR19973068.3542211/1‑150 (MQ=60)
                                             AGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTC                                                                                                          >  SRR19973068.1718400/1‑150 (MQ=60)
                                               CGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAT                                                                                                        >  SRR19973068.3472436/1‑150 (MQ=60)
                                               CGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAT                                                                                                        >  SRR19973068.4010441/1‑150 (MQ=60)
                                                  AACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTG                                                                                                     <  SRR19973068.2595324/150‑1 (MQ=60)
                                                  AACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTG                                                                                                     <  SRR19973068.3438679/150‑1 (MQ=60)
                                                  AACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTG                                                                                                     <  SRR19973068.2767687/150‑1 (MQ=60)
                                                  AACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTG                                                                                                     <  SRR19973068.3725138/150‑1 (MQ=60)
                                                    CTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGT                                                                                                   <  SRR19973068.3929235/150‑1 (MQ=60)
                                                      TCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTA                                                                                                 <  SRR19973068.827274/150‑1 (MQ=60)
                                                      TCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTA                                                                                                 <  SRR19973068.2899515/150‑1 (MQ=60)
                                                        AAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATT                                                                                               >  SRR19973068.3769631/1‑150 (MQ=60)
                                                         AATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTC                                                                                              >  SRR19973068.2057126/1‑150 (MQ=60)
                                                         AATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTC                                                                                              >  SRR19973068.2057099/1‑150 (MQ=60)
                                                            TGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAT                                                                                           <  SRR19973068.540972/150‑1 (MQ=60)
                                                             GCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATG                                                                                          >  SRR19973068.1252855/1‑150 (MQ=60)
                                                               AGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAG                                                                                        >  SRR19973068.1092118/1‑150 (MQ=60)
                                                                GCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGA                                                                                       >  SRR19973068.3686899/1‑150 (MQ=60)
                                                                GCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGA                                                                                       >  SRR19973068.3686958/1‑150 (MQ=60)
                                                                 CAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAT                                                                                      >  SRR19973068.107888/1‑150 (MQ=60)
                                                                 CAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAT                                                                                      <  SRR19973068.2395400/150‑1 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     <  SRR19973068.1222146/150‑1 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     >  SRR19973068.2251180/1‑150 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     <  SRR19973068.1222139/150‑1 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     <  SRR19973068.3139700/150‑1 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     <  SRR19973068.3139590/150‑1 (MQ=60)
                                                                  AAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATA                                                                                     <  SRR19973068.3139599/150‑1 (MQ=60)
                                                                   AAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAA                                                                                    >  SRR19973068.1215502/1‑150 (MQ=60)
                                                                    AAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAA                                                                                   >  SRR19973068.254788/1‑150 (MQ=60)
                                                                     AATGCCAGGCACACCGAGACAAAAGCCGCCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAG                                                                                  <  SRR19973068.3802783/150‑1 (MQ=60)
                                                                     AATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAG                                                                                  >  SRR19973068.1423187/1‑150 (MQ=60)
                                                                     AATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAG                                                                                  <  SRR19973068.3802740/150‑1 (MQ=60)
                                                                     AATGCAAGGAACACAGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCTGAGATAATAATCAGCGTCATAGCACAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAG                                                                                  <  SRR19973068.1750828/150‑1 (MQ=60)
                                                                      ATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGC                                                                                 >  SRR19973068.3799326/1‑150 (MQ=60)
                                                                      ATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGC                                                                                 <  SRR19973068.1843789/150‑1 (MQ=60)
                                                                       TGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCC                                                                                <  SRR19973068.808220/150‑1 (MQ=60)
                                                                        GCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCA                                                                               <  SRR19973068.3397452/150‑1 (MQ=60)
                                                                          CAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGG                                                                             >  SRR19973068.746973/1‑150 (MQ=60)
                                                                                   CGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAG                                                                    <  SRR19973068.3697485/150‑1 (MQ=60)
                                                                                      GACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGA                                                                 >  SRR19973068.3854144/1‑150 (MQ=60)
                                                                                         AAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAA                                                              <  SRR19973068.1633829/150‑1 (MQ=60)
                                                                                         AAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAA                                                              >  SRR19973068.2290429/1‑150 (MQ=60)
                                                                                                   ATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAA                                                    >  SRR19973068.2382206/1‑150 (MQ=60)
                                                                                                      GGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGA                                                 >  SRR19973068.3805847/1‑150 (MQ=60)
                                                                                                        CCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAT                                               <  SRR19973068.783590/150‑1 (MQ=60)
                                                                                                        CCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAT                                               <  SRR19973068.783342/150‑1 (MQ=60)
                                                                                                         CCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATG                                              >  SRR19973068.3144053/1‑150 (MQ=60)
                                                                                                         CCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATG                                              >  SRR19973068.3762740/1‑150 (MQ=60)
                                                                                                         CCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATG                                              <  SRR19973068.853714/150‑1 (MQ=60)
                                                                                                          CAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGG                                             <  SRR19973068.448789/150‑1 (MQ=60)
                                                                                                          CAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGG                                             <  SRR19973068.448592/150‑1 (MQ=60)
                                                                                                          CAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGG                                             <  SRR19973068.446913/150‑1 (MQ=60)
                                                                                                          CAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGG                                             <  SRR19973068.446877/150‑1 (MQ=60)
                                                                                                           AACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGC                                            <  SRR19973068.2970487/150‑1 (MQ=60)
                                                                                                               CTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATC                                        <  SRR19973068.734281/150‑1 (MQ=60)
                                                                                                               CTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATC                                        <  SRR19973068.734330/150‑1 (MQ=60)
                                                                                                               CTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATC                                        <  SRR19973068.734617/150‑1 (MQ=60)
                                                                                                                TGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCA                                       <  SRR19973068.1681115/150‑1 (MQ=60)
                                                                                                                TGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCA                                       <  SRR19973068.1681103/150‑1 (MQ=60)
                                                                                                                 GACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAC                                      <  SRR19973068.1567326/150‑1 (MQ=60)
                                                                                                                   CATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCA                                    >  SRR19973068.773396/1‑150 (MQ=60)
                                                                                                                   CATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCA                                    >  SRR19973068.3914674/1‑150 (MQ=60)
                                                                                                                      CAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCA                                 >  SRR19973068.2595711/1‑150 (MQ=60)
                                                                                                                      CAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCA                                 >  SRR19973068.2596332/1‑150 (MQ=60)
                                                                                                                       AATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAG                                >  SRR19973068.3360976/1‑150 (MQ=60)
                                                                                                                            CACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAC                           >  SRR19973068.428913/1‑150 (MQ=60)
                                                                                                                            CACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAC                           >  SRR19973068.428915/1‑150 (MQ=60)
                                                                                                                            CACAGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGAGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAC                           <  SRR19973068.2416956/150‑1 (MQ=60)
                                                                                                                             ACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACC                          >  SRR19973068.393686/1‑150 (MQ=60)
                                                                                                                             ACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACC                          <  SRR19973068.2414141/150‑1 (MQ=60)
                                                                                                                             ACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACC                          <  SRR19973068.2414138/150‑1 (MQ=60)
                                                                                                                                 GAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATC                      >  SRR19973068.4067717/1‑150 (MQ=60)
                                                                                                                                 GAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATC                      >  SRR19973068.2597923/1‑150 (MQ=60)
                                                                                                                                  AGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCT                     <  SRR19973068.982737/150‑1 (MQ=60)
                                                                                                                                  AGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCT                     <  SRR19973068.1586550/150‑1 (MQ=60)
                                                                                                                                   GATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTC                    >  SRR19973068.2749525/1‑150 (MQ=60)
                                                                                                                                      AATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATT                 >  SRR19973068.1054022/1‑150 (MQ=60)
                                                                                                                                         TATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAAT              >  SRR19973068.2756246/1‑150 (MQ=60)
                                                                                                                                         AATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAAT              <  SRR19973068.218677/150‑1 (MQ=60)
                                                                                                                                         AATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAAT              >  SRR19973068.2352311/1‑150 (MQ=60)
                                                                                                                                         AATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAAT              >  SRR19973068.2756208/1‑150 (MQ=60)
                                                                                                                                          ATCAGCGTCATAGCCCAGGCGGCAATCCCCATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATA             <  SRR19973068.3915089/150‑1 (MQ=60)
                                                                                                                                          ATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATA             <  SRR19973068.3915765/150‑1 (MQ=60)
                                                                                                                                            CAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATG           >  SRR19973068.3309713/1‑150 (MQ=60)
                                                                                                                                            CAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGACAGGCGCGTTCAGTGAGAAGACCAGATAAAGACTGGCAATCACCACCAGCAAACCGATCTCATTAATATG           >  SRR19973068.3309138/1‑150 (MQ=60)
                                                                                                                                                  CATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACT     <  SRR19973068.1523726/150‑1 (MQ=60)
                                                                                                                                                     AGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACTGAC  >  SRR19973068.1333317/1‑150 (MQ=60)
                                                                                                                                                     AGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACTGAC  >  SRR19973068.3393798/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACTGAC  >  CP009273/2669829‑2670127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑