Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 631,999 C→A 36.1% P9P (CCG→CCT)  ybdN ← PAPS reductase‑like domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273631,9990CA36.1% 384.8 / 247.4 251P9P (CCG→CCT) ybdNPAPS reductase‑like domain protein
Reads supporting (aligned to +/- strand):  ref base C (89/71);  new base A (47/44);  total (136/115)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTT  >  CP009273/631850‑632147
                                                                                                                                                     |                                                                                                                                                    
cAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGc                                                                                                                                                      >  1:658818/1‑150 (MQ=255)
 aGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCg                                                                                                                                                     <  1:1512976/150‑1 (MQ=255)
  gATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCgg                                                                                                                                                    <  2:4108436/150‑1 (MQ=255)
   aTTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGa                                                                                                                                                   <  2:2167110/150‑1 (MQ=255)
        tttGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAgg                                                                                                                                              >  1:1029667/1‑150 (MQ=255)
        tttGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAgg                                                                                                                                              >  1:3667644/1‑150 (MQ=255)
         ttGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGa                                                                                                                                             >  1:3074445/1‑150 (MQ=255)
          tGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAgaggaagaggaa                                                                                                                                            <  1:3694376/150‑1 (MQ=255)
           gCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAAt                                                                                                                                           >  2:4059621/1‑150 (MQ=255)
           gCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAAt                                                                                                                                           >  2:225971/1‑150 (MQ=255)
            cccATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGATAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAAtt                                                                                                                                          >  2:2825390/1‑150 (MQ=255)
               aTTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTta                                                                                                                                       >  2:1920119/1‑150 (MQ=255)
                   gTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATaaa                                                                                                                                   >  1:2065765/1‑150 (MQ=255)
                       ggCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGa                                                                                                                               >  1:2182337/1‑150 (MQ=255)
                        gCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGAc                                                                                                                              <  2:761400/150‑1 (MQ=255)
                        gCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGAc                                                                                                                              <  2:1137136/150‑1 (MQ=255)
                         cAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACa                                                                                                                             >  2:1284840/1‑150 (MQ=255)
                         cAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACa                                                                                                                             >  2:3749090/1‑150 (MQ=255)
                          aaGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACAt                                                                                                                            >  1:1695019/1‑150 (MQ=255)
                              tCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGa                                                                                                                        >  2:1411308/1‑150 (MQ=255)
                              tCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGa                                                                                                                        >  1:3454815/1‑150 (MQ=255)
                                ggCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAg                                                                                                                      <  1:3348134/150‑1 (MQ=255)
                                    gTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGc                                                                                                                  <  1:460712/150‑1 (MQ=255)
                                    gTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGc                                                                                                                  >  2:4067690/1‑150 (MQ=255)
                                       aGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGctct                                                                                                               <  2:4120349/150‑1 (MQ=255)
                                        gATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGctctc                                                                                                              <  2:3851482/150‑1 (MQ=255)
                                         aTGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGctctct                                                                                                             >  2:3658077/1‑150 (MQ=255)
                                          tGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTg                                                                                                            <  2:3584577/150‑1 (MQ=255)
                                          tGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTg                                                                                                            <  2:3054827/150‑1 (MQ=255)
                                              gcaTTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACg                                                                                                        >  1:1351834/1‑150 (MQ=255)
                                                aTTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGtt                                                                                                      >  2:293768/1‑150 (MQ=255)
                                                  tAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTAc                                                                                                    <  2:2949164/150‑1 (MQ=255)
                                                  aaaaCCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGGGTTGATTGTCCAGGTAATACGCTCACGTGCAGCTTCCAGAATAGTGAGCGGAAGAGGAAATTTATAAATAGACAACTGAAGACGCTCTCGGAACGTTAc                                                                                                    >  2:1027564/2‑150 (MQ=255)
                                                   aaaCCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACa                                                                                                   <  1:2037532/150‑1 (MQ=255)
                                                   aaaCCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACa                                                                                                   >  2:3285597/1‑150 (MQ=255)
                                                      ccAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAggg                                                                                                <  1:1279019/150‑1 (MQ=255)
                                                       cAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGt                                                                                               <  2:3817576/150‑1 (MQ=255)
                                                       cAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGt                                                                                               <  2:2372314/150‑1 (MQ=255)
                                                         gagTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGa                                                                                             >  1:1419383/1‑150 (MQ=255)
                                                           gTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATc                                                                                           <  1:644964/150‑1 (MQ=255)
                                                            tCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCa                                                                                          <  2:2914746/150‑1 (MQ=255)
                                                                   cgccAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGAt                                                                                   <  2:2746679/150‑1 (MQ=255)
                                                                      cAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAg                                                                                >  1:470503/1‑150 (MQ=255)
                                                                          aaaTGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTg                                                                            >  2:1344415/1‑150 (MQ=255)
                                                                           aaTGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGt                                                                           >  2:466863/1‑150 (MQ=255)
                                                                           aaTGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGt                                                                           >  1:3867890/1‑150 (MQ=255)
                                                                            aTGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGtt                                                                          <  1:1649080/150‑1 (MQ=255)
                                                                            aTGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGtt                                                                          <  1:1648793/150‑1 (MQ=255)
                                                                              gagaCACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTaa                                                                        >  2:3229012/1‑150 (MQ=255)
                                                                              gagaCACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTaa                                                                        >  2:409079/1‑150 (MQ=255)
                                                                               agaCACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAAt                                                                       <  2:907883/150‑1 (MQ=255)
                                                                                gaCACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAAtt                                                                      <  1:2004702/150‑1 (MQ=255)
                                                                                 acacAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATta                                                                     <  2:940693/150‑1 (MQ=255)
                                                                                      aaCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAg                                                                >  1:2318741/1‑150 (MQ=255)
                                                                                      aaCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAg                                                                >  1:1850464/1‑150 (MQ=255)
                                                                                       aCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGt                                                               >  2:2585055/1‑150 (MQ=255)
                                                                                       aCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGt                                                               <  1:244461/150‑1 (MQ=255)
                                                                                       aCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGt                                                               <  1:244421/150‑1 (MQ=255)
                                                                                       aCGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGt                                                               <  1:1926770/150‑1 (MQ=255)
                                                                                        cgcgGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGGGAGATAAGTCTGTTAATTATAAAGTa                                                              >  1:944961/1‑150 (MQ=255)
                                                                                            gggTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAaca                                                          >  2:4147489/1‑150 (MQ=255)
                                                                                            gggTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAaca                                                          >  1:1754886/1‑150 (MQ=255)
                                                                                              gTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACt                                                        <  2:2360315/150‑1 (MQ=255)
                                                                                               tAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTg                                                       >  1:1566959/1‑150 (MQ=255)
                                                                                               tAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTg                                                       >  2:1812669/1‑150 (MQ=255)
                                                                                                  ggTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTaa                                                    >  2:2660106/1‑150 (MQ=255)
                                                                                                     gtTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGc                                                 <  2:3520104/150‑1 (MQ=255)
                                                                                                       tGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCag                                               <  2:718978/150‑1 (MQ=255)
                                                                                                       tGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCag                                               >  1:2682411/1‑150 (MQ=255)
                                                                                                         aGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCagag                                             <  1:724696/150‑1 (MQ=255)
                                                                                                          gtgtCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAgt                                            >  2:1587600/1‑150 (MQ=255)
                                                                                                             tCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGtt                                         <  2:3976230/150‑1 (MQ=255)
                                                                                                             tCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGtt                                         >  1:807852/1‑150 (MQ=255)
                                                                                                              ccAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGttt                                        <  2:1624678/150‑1 (MQ=255)
                                                                                                              ccAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGttt                                        <  1:1549786/150‑1 (MQ=255)
                                                                                                                aGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTa                                      >  1:1334471/1‑150 (MQ=255)
                                                                                                                aGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTa                                      >  2:2445444/1‑150 (MQ=255)
                                                                                                                aGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAAATTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATAATAAAGTAAACACTGTAAAGCAGAGTGTTTTa                                      >  2:512237/1‑150 (MQ=255)
                                                                                                                   tAATACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTAttt                                   <  1:2888586/150‑1 (MQ=255)
                                                                                                                    aaTACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTAtttt                                  >  1:3987096/1‑150 (MQ=255)
                                                                                                                     aTACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTAttttt                                 >  1:3573079/1‑150 (MQ=255)
                                                                                                                     aTACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGGTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTAttttt                                 >  1:3062067/1‑150 (MQ=255)
                                                                                                                      tACGCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTc                                <  1:785874/150‑1 (MQ=255)
                                                                                                                        cGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCAt                              <  1:3910048/150‑1 (MQ=255)
                                                                                                                         gCTCTCGTGCAGCTTCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCAtt                             >  1:2966903/1‑150 (MQ=255)
                                                                                                                           tctcGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTcg                           >  1:428762/1‑150 (MQ=255)
                                                                                                                              cGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCgcgc                        >  2:592568/1‑150 (MQ=255)
                                                                                                                              cGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCgcgc                        >  2:1839947/1‑150 (MQ=255)
                                                                                                                               gTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAACAGAGTGTTTTATTTTTCATTCGCGCt                       <  1:326180/150‑1 (MQ=255)
                                                                                                                                  cAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCtgtg                    >  2:836319/1‑150 (MQ=255)
                                                                                                                                   aGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGa                   >  1:1885845/1‑150 (MQ=255)
                                                                                                                                      ttCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGaata                >  1:1146664/1‑150 (MQ=255)
                                                                                                                                      ttCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGaata                >  1:3121228/1‑150 (MQ=255)
                                                                                                                                      ttCCAGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGaata                >  1:3238436/1‑150 (MQ=255)
                                                                                                                                          aGAATATTGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGaataaata            <  1:1216182/150‑1 (MQ=255)
                                                                                                                                            aaTATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGa          <  1:4082895/150‑1 (MQ=255)
                                                                                                                                                 tGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGaaa     >  2:3118371/1‑150 (MQ=255)
                                                                                                                                                 tGAGAGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGAAGAGAGTTTTATTTTTCATTCTCGCTGTGAATAAATAGAAGaaa     >  2:773131/1‑150 (MQ=255)
                                                                                                                                                  gagaGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAg    >  2:3694476/1‑150 (MQ=255)
                                                                                                                                                   aGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACCGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAATGt   >  2:3337979/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTT  >  CP009273/631850‑632147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A