Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,788,981 A→T 41.6% S143R (AGT→AGA)  btuC ← vitamin B12 transporter subunit: membrane component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,788,9810AT41.6% 158.3 / inf 238S143R (AGT→AGA) btuCvitamin B12 transporter subunit: membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (76/63);  new base T (56/43);  total (132/106)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 238 total aligned reads displayed.

CCAGCTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTA  >  CP009273/1788834‑1789128
                                                                                                                                                   |                                                                                                                                                   
ccagcTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACt                                                                                                                                                   <  2:3207799/150‑1 (MQ=255)
 cagcTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTg                                                                                                                                                  <  1:211827/150‑1 (MQ=255)
 cagcTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTg                                                                                                                                                  >  2:2431886/1‑150 (MQ=255)
  agcTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTgg                                                                                                                                                 >  1:712611/1‑150 (MQ=255)
    cTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTc                                                                                                                                               <  1:3020545/150‑1 (MQ=255)
    cTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTc                                                                                                                                               >  1:1114628/1‑150 (MQ=255)
    cTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTc                                                                                                                                               <  1:309106/150‑1 (MQ=255)
    cTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTc                                                                                                                                               <  1:3990614/150‑1 (MQ=255)
         ccgccAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAg                                                                                                                                          <  2:1353960/150‑1 (MQ=255)
          cgccAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGa                                                                                                                                         >  1:1946189/1‑150 (MQ=255)
           gccAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCCCGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGAt                                                                                                                                        <  2:3536243/150‑1 (MQ=255)
            ccAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATg                                                                                                                                       >  2:2524559/1‑150 (MQ=255)
             cAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATga                                                                                                                                      >  2:1186647/1‑150 (MQ=255)
             cAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATga                                                                                                                                      <  2:1402098/150‑1 (MQ=255)
              aGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATgac                                                                                                                                     <  1:1540894/150‑1 (MQ=255)
              aGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATgac                                                                                                                                     <  1:1900899/150‑1 (MQ=255)
              aGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATgac                                                                                                                                     <  2:4022174/150‑1 (MQ=255)
              aGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATgac                                                                                                                                     <  1:646702/150‑1 (MQ=255)
                 cTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATgacgac                                                                                                                                  >  1:2388636/1‑150 (MQ=255)
                      ccgccAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCg                                                                                                                             <  1:1142780/150‑1 (MQ=255)
                        acaaaaaCCGACCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGACAACGTCATTAGTTCGATACAGATAATCACTAATGAAACGCCAGCCAGCAATAACCTACTTGTCGAAATATGACTAAGGGCGaa                                                                                                                           >  2:782614/4‑150 (MQ=255)
                          cAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAAc                                                                                                                         >  1:231745/1‑150 (MQ=255)
                           aaaaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACg                                                                                                                        <  1:418472/150‑1 (MQ=255)
                           aaaaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACg                                                                                                                        >  1:2555867/1‑150 (MQ=255)
                           aaaaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACg                                                                                                                        >  2:3833215/1‑150 (MQ=255)
                           aaaaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACg                                                                                                                        >  1:986521/1‑150 (MQ=255)
                            aaaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGt                                                                                                                       <  1:2341329/150‑1 (MQ=255)
                             aaCCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTa                                                                                                                      <  2:1834604/150‑1 (MQ=255)
                                   ccATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTCAGAGTa                                                                                                                >  2:3584281/1‑150 (MQ=255)
                                    catcatCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTAt                                                                                                               <  1:3128591/150‑1 (MQ=255)
                                     atcatcCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTAtt                                                                                                              <  2:3091390/150‑1 (MQ=255)
                                     atcatcCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTAtt                                                                                                              <  1:3872807/150‑1 (MQ=255)
                                       catcCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTaa                                                                                                            >  2:2067897/1‑150 (MQ=255)
                                        atcCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTaaa                                                                                                           >  2:3138050/1‑150 (MQ=255)
                                         tcCTGTACATCAGCTGACGAAAATCAACTGAGGTGGATAAGTAGATAGCCCACTTCATTAGTGCTCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGTGTATTAAAg                                                                                                          <  2:2823908/150‑1 (MQ=255)
                                          ccAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGt                                                                                                         <  2:2543417/150‑1 (MQ=255)
                                           cAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGtg                                                                                                        >  1:3992526/1‑150 (MQ=255)
                                           cAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGtg                                                                                                        >  1:2731485/1‑150 (MQ=255)
                                               aCATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGACGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGtgatga                                                                                                    >  1:2400169/1‑150 (MQ=255)
                                                cATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTgatgat                                                                                                   >  1:4787/1‑150 (MQ=255)
                                                cATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTgatgat                                                                                                   >  2:2665020/1‑150 (MQ=255)
                                                 aTCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATa                                                                                                  >  2:1984620/1‑150 (MQ=255)
                                                 aTCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATa                                                                                                  <  1:1548357/150‑1 (MQ=255)
                                                  tCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATaa                                                                                                 <  2:111370/150‑1 (MQ=255)
                                                    aGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAgc                                                                                               >  2:1754084/1‑150 (MQ=255)
                                                         aCGCAAATAAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTTCAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAAAGTAAGAGTATTAAAGTGATGATAAGCGCGcc                                                                                          <  1:1037271/150‑1 (MQ=255)
                                                          cGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCa                                                                                         >  1:3292536/1‑150 (MQ=255)
                                                          cGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCa                                                                                         >  1:3507380/1‑150 (MQ=255)
                                                          cGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCa                                                                                         <  2:708258/150‑1 (MQ=255)
                                                           gCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAg                                                                                        >  1:2654080/1‑150 (MQ=255)
                                                               aTCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAAt                                                                                    <  2:1903297/150‑1 (MQ=255)
                                                                  aaCTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGc                                                                                 >  2:3626944/1‑150 (MQ=255)
                                                                     tGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGAATTAAAGTGATGATAAGCGCGCCAGCAATCGcaca                                                                              >  2:2616811/1‑150 (MQ=255)
                                                                      gAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGcacac                                                                             >  1:86575/1‑150 (MQ=255)
                                                                      gAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGcacac                                                                             >  1:2540352/1‑150 (MQ=255)
                                                                        ggTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAg                                                                           >  1:2014523/1‑150 (MQ=255)
                                                                           ggAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGccc                                                                        >  1:3506045/1‑150 (MQ=255)
                                                                            gAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCt                                                                       <  1:2793856/150‑1 (MQ=255)
                                                                             aaaaGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTa                                                                      <  1:3865348/150‑1 (MQ=255)
                                                                                       aGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGtt                                                            >  2:110267/1‑150 (MQ=255)
                                                                                        gCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTg                                                           >  2:207607/1‑150 (MQ=255)
                                                                                        gCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTg                                                           >  1:3700906/1‑150 (MQ=255)
                                                                                            aCGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGa                                                       >  2:1615651/1‑150 (MQ=255)
                                                                                             cGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAg                                                      >  2:52386/1‑150 (MQ=255)
                                                                                             cGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAg                                                      <  2:619146/150‑1 (MQ=255)
                                                                                             cGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAg                                                      >  2:2272132/1‑150 (MQ=255)
                                                                                               tCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGtt                                                    <  2:3675931/150‑1 (MQ=255)
                                                                                               tCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGtt                                                    <  2:3942177/150‑1 (MQ=255)
                                                                                               tCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGtt                                                    <  2:3804714/150‑1 (MQ=255)
                                                                                                cATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttg                                                   >  2:1025509/1‑150 (MQ=255)
                                                                                                 aTTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgc                                                  >  2:3577172/1‑150 (MQ=255)
                                                                                                    aGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgccct                                               >  1:1447025/1‑150 (MQ=255)
                                                                                                      tGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttg                                             >  2:2109111/1‑150 (MQ=255)
                                                                                                          cTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCa                                         >  1:2546950/1‑150 (MQ=255)
                                                                                                              aGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCa                                     >  2:2341433/1‑150 (MQ=255)
                                                                                                                aTAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAAt                                   >  1:2975703/1‑150 (MQ=255)
                                                                                                                  aaTCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATAc                                 <  2:912840/150‑1 (MQ=255)
                                                                                                                      ccTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                             >  2:2492702/1‑150 (MQ=255)
                                                                                                                       cTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCgcgg                            >  1:3832423/1‑150 (MQ=255)
                                                                                                                        tAATGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCgcggc                           <  1:4068729/150‑1 (MQ=255)
                                                                                                                         aaTGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCgcggcg                          >  2:3697233/1‑150 (MQ=255)
                                                                                                                           tGCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGAt                        <  2:3004712/150‑1 (MQ=255)
                                                                                                                            gCAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATACGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATa                       >  2:3263434/1‑150 (MQ=255)
                                                                                                                            gCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAACGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATa                       <  1:1320591/150‑1 (MQ=255)
                                                                                                                             cAACGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATaa                      >  2:1429475/1‑150 (MQ=255)
                                                                                                                             cAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCACTACCGCGGCGATaa                      >  2:1028875/1‑150 (MQ=255)
                                                                                                                              aaCGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAg                     >  1:812756/1‑150 (MQ=255)
                                                                                                                              aaCGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCACACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAg                     >  1:337238/1‑150 (MQ=255)
                                                                                                                               aCGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGc                    >  2:2998351/1‑150 (MQ=255)
                                                                                                                                cGCCAGCCAGCAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGcc                   <  2:1538026/150‑1 (MQ=255)
                                                                                                                                  ccagccagCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGcccc                 <  1:3383966/150‑1 (MQ=255)
                                                                                                                                   cagccagcAATAACCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTCGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCa                >  2:2786235/1‑150 (MQ=255)
                                                                                                                                    agccagcAATAACCGTCTGGACGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGATGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCAc               >  2:2468705/1‑150 (MQ=255)
                                                                                                                                    agccagcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCAc               >  2:1265300/1‑150 (MQ=255)
                                                                                                                                        agcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATACGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCt           <  2:1787245/150‑1 (MQ=255)
                                                                                                                                        agcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCt           >  2:2665956/1‑150 (MQ=255)
                                                                                                                                              aaCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCg     >  2:3511545/1‑150 (MQ=255)
                                                                                                                                              aaCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCg     >  2:3797710/1‑150 (MQ=255)
                                                                                                                                               aCCGTCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGt    <  2:2751525/150‑1 (MQ=255)
                                                                                                                                               aCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGt    <  1:133842/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAGCTTTGCCGCCAGTCTACGCCGCCAAAACCGCCCATCATCCAGTACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTA  >  CP009273/1788834‑1789128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A