Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,771,358 G→A 100% intergenic (+217/+147) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,771,3580GA99.1% 740.1 / ‑2.3 214intergenic (+217/+147)ypjF/ypjACP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (120/92);  total (122/92)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

AACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATG  >  CP009273/2771211‑2771503
                                                                                                                                                   |                                                                                                                                                 
aaCCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAgt                                                                                                                                                 >  1:3039403/1‑150 (MQ=255)
     cccctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAg                                                                                                                                            <  2:3056076/150‑1 (MQ=255)
         tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGaca                                                                                                                                        >  2:2548585/1‑150 (MQ=255)
         tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGaca                                                                                                                                        <  2:1259283/150‑1 (MQ=255)
         tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGaca                                                                                                                                        >  2:3220724/1‑150 (MQ=255)
         tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGaca                                                                                                                                        <  2:2370656/150‑1 (MQ=255)
          cccccGAAAAAACCTTACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAAACAATTTAACAACAAGTTACACAACCAATTCAGACACCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaa                                                                                                                                       >  1:2220921/1‑150 (MQ=255)
          cccccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaa                                                                                                                                       <  2:3928751/150‑1 (MQ=255)
          cccccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaa                                                                                                                                       >  2:3754751/1‑150 (MQ=255)
           ccccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaac                                                                                                                                      >  2:1316276/1‑150 (MQ=255)
            cccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGacaaca                                                                                                                                     >  2:107406/1‑150 (MQ=255)
             ccGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAacac                                                                                                                                    <  2:568045/150‑1 (MQ=255)
                aaaaaaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaa                                                                                                                                 <  2:174449/150‑1 (MQ=255)
                   aaaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACcaacaa                                                                                                                              >  2:4139410/1‑150 (MQ=255)
                    aaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACaaa                                                                                                                             <  1:1847781/150‑1 (MQ=255)
                    aaCCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACaaa                                                                                                                             >  2:188762/1‑150 (MQ=255)
                       cTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAAc                                                                                                                          >  2:4128252/1‑150 (MQ=255)
                       cTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAAc                                                                                                                          >  1:3798841/1‑150 (MQ=255)
                        tGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAATCa                                                                                                                         >  1:2694161/1‑150 (MQ=255)
                        tGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACa                                                                                                                         >  1:2617567/1‑150 (MQ=255)
                          aCATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAgg                                                                                                                       <  2:2528402/150‑1 (MQ=255)
                            aTTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGaa                                                                                                                     >  2:1020172/1‑150 (MQ=255)
                              tttCTTTTAGGACCAACAATGTGACAAAAATGAAAATTGAACTGAAGATTACTATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGt                                                                                                                   <  1:1121944/150‑1 (MQ=255)
                                   tttAGGACCAACAATGGGACCAAAATGAAAATTGATCTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGGCAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGaa                                                                                                              <  1:3471981/150‑1 (MQ=255)
                                   tttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGGCAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGaa                                                                                                              <  1:1377449/150‑1 (MQ=255)
                                      aGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGtc                                                                                                           >  2:421115/1‑150 (MQ=255)
                                       ggACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGtct                                                                                                          >  1:1037782/1‑150 (MQ=255)
                                        gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGtctc                                                                                                         >  2:115771/1‑150 (MQ=255)
                                         aCCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCa                                                                                                        <  2:1590148/150‑1 (MQ=255)
                                           caacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAAGCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGc                                                                                                      >  2:4023078/1‑150 (MQ=255)
                                            aacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCa                                                                                                     >  1:3065198/1‑150 (MQ=255)
                                             acaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCaa                                                                                                    <  2:1551027/150‑1 (MQ=255)
                                               aaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCaaaa                                                                                                  >  2:2226315/1‑150 (MQ=255)
                                               aaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCaaaa                                                                                                  >  1:2064320/1‑150 (MQ=255)
                                                 tGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAaca                                                                                                <  2:2189069/150‑1 (MQ=255)
                                                    gACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCg                                                                                             <  2:4094209/150‑1 (MQ=255)
                                                     aCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGa                                                                                            <  1:2441042/150‑1 (MQ=255)
                                                     aCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGa                                                                                            <  1:360019/150‑1 (MQ=255)
                                                      ccAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAc                                                                                           >  2:2546858/1‑150 (MQ=255)
                                                       cAAAATGAAAATTGAACTGAAGCTTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                                                                                          <  1:324263/150‑1 (MQ=255)
                                                        aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  2:3714280/150‑1 (MQ=255)
                                                        aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  1:4168835/150‑1 (MQ=255)
                                                        aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACAAAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                                                                                         <  2:3241669/150‑1 (MQ=255)
                                                         aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAg                                                                                        <  2:2057738/150‑1 (MQ=255)
                                                         aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAg                                                                                        >  2:661701/1‑150 (MQ=255)
                                                          aaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGa                                                                                       <  1:588892/150‑1 (MQ=255)
                                                          aaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGa                                                                                       <  1:2669270/150‑1 (MQ=255)
                                                          aaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGa                                                                                       <  1:2669548/150‑1 (MQ=255)
                                                           aTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGACAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGAc                                                                                      <  2:1492609/150‑1 (MQ=255)
                                                              aaaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGt                                                                                   <  1:2387017/150‑1 (MQ=255)
                                                               aaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGTTTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTg                                                                                  <  1:3408536/150‑1 (MQ=255)
                                                                aaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTga                                                                                 >  2:2336773/1‑150 (MQ=255)
                                                                aaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTga                                                                                 >  1:1217418/1‑150 (MQ=255)
                                                                 aTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGATGTCTCAGCAAAACACCGACCAGACGGTgag                                                                                <  2:607129/150‑1 (MQ=255)
                                                                 aTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgag                                                                                >  2:1193025/1‑150 (MQ=255)
                                                                 aTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgag                                                                                <  1:2132061/150‑1 (MQ=255)
                                                                   tGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAATCAGGAAGTTCGAAGTCTCAGCAAAACACCGACCAGACGGTgagga                                                                              >  1:172519/1‑150 (MQ=255)
                                                                    gAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgaggag                                                                             >  1:2463237/1‑150 (MQ=255)
                                                                     aaCTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGgaga                                                                            >  2:1675051/1‑150 (MQ=255)
                                                                          aaGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATaa                                                                       >  1:1828583/1‑150 (MQ=255)
                                                                             aTTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAg                                                                    >  2:2268697/1‑150 (MQ=255)
                                                                                  aatcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATAc                                                               >  2:1336366/1‑150 (MQ=255)
                                                                                   aacaatTTAACAACAAGTTACACAAACAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACATTGTAAGGACAACACCAACAAAAACATGAAGTTAGAAGTCTCAGCAAAACACCGACAAGACGGTGAGGAGACATAAAAGCATACg                                                              >  1:775324/3‑150 (MQ=255)
                                                                                    tcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGc                                                             >  1:262254/1‑150 (MQ=255)
                                                                                      aatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCaa                                                           >  1:2808361/1‑150 (MQ=255)
                                                                                      aatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCaa                                                           >  1:2501864/1‑150 (MQ=255)
                                                                                       atTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCaaa                                                          >  2:3981705/1‑150 (MQ=255)
                                                                                         ttAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAgg                                                        >  2:16908/1‑150 (MQ=255)
                                                                                         ttAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAgg                                                        <  1:672895/150‑1 (MQ=255)
                                                                                          tAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCACAGGa                                                       >  2:240350/1‑150 (MQ=255)
                                                                                          tAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGa                                                       <  2:3344915/150‑1 (MQ=255)
                                                                                           aacaacAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAg                                                      >  1:2278959/1‑150 (MQ=255)
                                                                                             caacaaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGcc                                                    >  2:3734117/1‑150 (MQ=255)
                                                                                              aacaaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCcg                                                   <  1:1470158/150‑1 (MQ=255)
                                                                                                caaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCcgcg                                                 <  1:638517/150‑1 (MQ=255)
                                                                                                 aaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCgg                                                >  1:1071591/1‑150 (MQ=255)
                                                                                                 aaGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCgg                                                >  1:457921/1‑150 (MQ=255)
                                                                                                   gTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCt                                              >  1:2011641/1‑150 (MQ=255)
                                                                                                     tACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTcc                                            >  1:2985856/1‑150 (MQ=255)
                                                                                                      acacAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACCGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCt                                           <  2:2667048/150‑1 (MQ=255)
                                                                                                       cacaACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTg                                          >  1:2820420/1‑150 (MQ=255)
                                                                                                       cacaACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACAACAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAATGAGCCGCGGCTCCTg                                          >  1:544785/1‑150 (MQ=255)
                                                                                                          aaCCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTa                                       >  2:2417670/1‑150 (MQ=255)
                                                                                                          aaCCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTa                                       <  1:1260177/150‑1 (MQ=255)
                                                                                                                  cAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCACAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTCACATGaaa                               >  2:3411858/1‑150 (MQ=255)
                                                                                                                  cAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGaaa                               <  2:1959636/150‑1 (MQ=255)
                                                                                                                   aGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGACGTTCGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAg                              >  2:3739089/1‑150 (MQ=255)
                                                                                                                        ccGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCcac                         >  1:2673636/1‑150 (MQ=255)
                                                                                                                            cAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAt                     <  1:2862265/150‑1 (MQ=255)
                                                                                                                             aGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtg                    >  1:2569805/1‑150 (MQ=255)
                                                                                                                             aGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtg                    >  1:1169706/1‑150 (MQ=255)
                                                                                                                             aGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtg                    >  2:2496261/1‑150 (MQ=255)
                                                                                                                              gCCCACCAATAATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtgt                   <  2:2865228/150‑1 (MQ=255)
                                                                                                                               cccACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAACCAGGAAGTTAGAAGTCTCAGCAAAACACCGACCCGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAtgtg                  <  2:2300801/150‑1 (MQ=255)
                                                                                                                                ccaccaATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACAT‑AAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTggg                <  1:2244362/150‑1 (MQ=255)
                                                                                                                                  accaATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGc               <  1:4053420/150‑1 (MQ=255)
                                                                                                                                        cATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTc         >  1:3328191/1‑150 (MQ=255)
                                                                                                                                         aTGATTGGACAGTGTAAGGACAACACCAACAAAAACAGTAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCg        <  1:1122901/150‑1 (MQ=255)
                                                                                                                                              tGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGAt   >  1:769792/1‑150 (MQ=255)
                                                                                                                                               ggACAGTGTAAGGACAACACCAACAAAAACAGGAACTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGTATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATg  <  2:2881449/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATG  >  CP009273/2771211‑2771503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTC  >  CP009273/2771209‑2771506
                                                                                                                                                     |                                                                                                                                                    
GTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACA                                                                                                                                                      >  SRR19973074.2894381/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     >  SRR19973074.644760/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     >  SRR19973074.644721/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     <  SRR19973074.2755343/150‑1 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     <  SRR19973074.1925107/150‑1 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     >  SRR19973074.2328724/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     >  SRR19973074.2328723/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     >  SRR19973074.1893995/1‑150 (MQ=60)
 TAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTGTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAG                                                                                                                                                     <  SRR19973074.1925215/150‑1 (MQ=60)
  AACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGT                                                                                                                                                    >  SRR19973074.3045025/1‑150 (MQ=60)
     CTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTA                                                                                                                                                 >  SRR19973074.3051399/1‑150 (MQ=60)
         CCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGA                                                                                                                                             <  SRR19973074.1138241/150‑1 (MQ=60)
           TCCCCCTAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACA                                                                                                                                           <  SRR19973074.2773473/150‑1 (MQ=60)
            CCCCCGAAAAAACCTTACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAAACAATTTAACAACAAGTTACACAACCAATTCAGACACCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAA                                                                                                                                          >  SRR19973074.2225100/1‑150 (MQ=60)
            CCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAA                                                                                                                                          >  SRR19973074.2225107/1‑150 (MQ=60)
            CCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATACAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAG                                                                                                                                          >  SRR19973074.2225092/1‑150 (MQ=60)
                CGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACC                                                                                                                                      >  SRR19973074.4100368/1‑150 (MQ=60)
                  AAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAA                                                                                                                                    >  SRR19973074.1901157/1‑150 (MQ=60)
                      AACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAA                                                                                                                                <  SRR19973074.1851310/150‑1 (MQ=60)
                      AACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAA                                                                                                                                <  SRR19973074.1848098/150‑1 (MQ=60)
                        CCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAA                                                                                                                              <  SRR19973074.1695205/150‑1 (MQ=60)
                         CTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAAC                                                                                                                             >  SRR19973074.3805799/1‑150 (MQ=60)
                          TGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAATCA                                                                                                                            >  SRR19973074.2699165/1‑150 (MQ=60)
                          TGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACA                                                                                                                            >  SRR19973074.2622464/1‑150 (MQ=60)
                              ATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAA                                                                                                                        <  SRR19973074.2819201/150‑1 (MQ=60)
                                TTTCTTTTAGGACCAACAATGTGACAAAAATGAAAATTGAACTGAAGATTACTATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGT                                                                                                                      <  SRR19973074.1124060/150‑1 (MQ=60)
                                  TCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTA                                                                                                                    >  SRR19973074.604660/1‑150 (MQ=60)
                                     TTTAGGACCAACAATGGGACCAAAATGAAAATTGATCTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGGCAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAA                                                                                                                 <  SRR19973074.3478360/150‑1 (MQ=60)
                                     TTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGGCAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAA                                                                                                                 <  SRR19973074.1379994/150‑1 (MQ=60)
                                     TTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAA                                                                                                                 <  SRR19973074.3038637/150‑1 (MQ=60)
                                         GGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCT                                                                                                             >  SRR19973074.1039692/1‑150 (MQ=60)
                                           ACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCA                                                                                                           <  SRR19973074.2841212/150‑1 (MQ=60)
                                            CCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAG                                                                                                          >  SRR19973074.1069843/1‑150 (MQ=60)
                                              AACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCA                                                                                                        >  SRR19973074.3070848/1‑150 (MQ=60)
                                                 AATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAA                                                                                                     >  SRR19973074.2068195/1‑150 (MQ=60)
                                                   TGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACA                                                                                                   <  SRR19973074.1173755/150‑1 (MQ=60)
                                                       ACCAAAATGATAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGTCTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACTAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGA                                                                                               <  SRR19973074.2445578/150‑1 (MQ=60)
                                                       ACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGA                                                                                               <  SRR19973074.360679/150‑1 (MQ=60)
                                                       ACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGA                                                                                               <  SRR19973074.2445560/150‑1 (MQ=60)
                                                         CAAAATGAAAATTGAACTGAAGCTTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACC                                                                                             <  SRR19973074.324855/150‑1 (MQ=60)
                                                         CAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACC                                                                                             >  SRR19973074.2697718/1‑150 (MQ=60)
                                                          AAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCA                                                                                            <  SRR19973074.4176498/150‑1 (MQ=60)
                                                           AAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAG                                                                                           <  SRR19973074.3661266/150‑1 (MQ=60)
                                                            AATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGA                                                                                          <  SRR19973074.2677904/150‑1 (MQ=60)
                                                            AATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGA                                                                                          <  SRR19973074.2674521/150‑1 (MQ=60)
                                                            AATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGA                                                                                          <  SRR19973074.2674251/150‑1 (MQ=60)
                                                            AATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGA                                                                                          <  SRR19973074.590028/150‑1 (MQ=60)
                                                            AATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGA                                                                                          <  SRR19973074.2674243/150‑1 (MQ=60)
                                                               GAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGG                                                                                       <  SRR19973074.1386168/150‑1 (MQ=60)
                                                                AAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGT                                                                                      <  SRR19973074.1819616/150‑1 (MQ=60)
                                                                AAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGT                                                                                      <  SRR19973074.2391443/150‑1 (MQ=60)
                                                                AAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGT                                                                                      <  SRR19973074.2391445/150‑1 (MQ=60)
                                                                 AAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGTTTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTG                                                                                     <  SRR19973074.3414837/150‑1 (MQ=60)
                                                                  AATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGA                                                                                    >  SRR19973074.1219752/1‑150 (MQ=60)
                                                                   ATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACACAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAG                                                                                   >  SRR19973074.2470883/1‑150 (MQ=60)
                                                                   ATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAG                                                                                   <  SRR19973074.2136044/150‑1 (MQ=60)
                                                                   ATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAG                                                                                   <  SRR19973074.2136063/150‑1 (MQ=60)
                                                                     TGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAATCAGGAAGTTCGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGA                                                                                 >  SRR19973074.172897/1‑150 (MQ=60)
                                                                      GAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAG                                                                                >  SRR19973074.2467801/1‑150 (MQ=60)
                                                                       AACTGAAGATTACAATTAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGA                                                                               >  SRR19973074.825895/1‑150 (MQ=60)
                                                                       AACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGA                                                                               >  SRR19973074.45087/1‑150 (MQ=60)
                                                                         CTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACA                                                                             <  SRR19973074.421930/150‑1 (MQ=60)
                                                                          TGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACAT                                                                            <  SRR19973074.263650/150‑1 (MQ=60)
                                                                            AAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAA                                                                          >  SRR19973074.1394789/1‑150 (MQ=60)
                                                                            AAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAA                                                                          >  SRR19973074.1832072/1‑150 (MQ=60)
                                                                                     ATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACG                                                                 <  SRR19973074.2219102/150‑1 (MQ=60)
                                                                                     ATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACG                                                                 >  SRR19973074.776777/1‑150 (MQ=60)
                                                                                     AACAATTTAACAACAAGTTACACAAACAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACATTGTAAGGACAACACCAACAAAAACATGAAGTTAGAAGTCTCAGCAAAACACCGACAAGACGGTGAGGAGACATAAAAGCATACG                                                                 >  SRR19973074.776810/1‑150 (MQ=60)
                                                                                      TCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGC                                                                >  SRR19973074.262771/1‑150 (MQ=60)
                                                                                      TCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGC                                                                <  SRR19973074.2054219/150‑1 (MQ=60)
                                                                                        AATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTACGGACAACACCAACAAAAAAAGGAAGTTAGAAGTCTCAGCAAATCACCGACCAGACGGTGAGGAGACATAAAAGGATACGAAA                                                              >  SRR19973074.2506530/1‑150 (MQ=60)
                                                                                        AATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAA                                                              >  SRR19973074.2813547/1‑150 (MQ=60)
                                                                                        AATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAA                                                              >  SRR19973074.2506517/1‑150 (MQ=60)
                                                                                           TTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACCGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGG                                                           <  SRR19973074.674048/150‑1 (MQ=60)
                                                                                           TTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGG                                                           <  SRR19973074.674134/150‑1 (MQ=60)
                                                                                             AACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAG                                                         >  SRR19973074.540469/1‑150 (MQ=60)
                                                                                             AACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAG                                                         >  SRR19973074.2283238/1‑150 (MQ=60)
                                                                                             AACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAG                                                         >  SRR19973074.2283162/1‑150 (MQ=60)
                                                                                                AACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCG                                                      >  SRR19973074.3982186/1‑150 (MQ=60)
                                                                                                AACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCG                                                      <  SRR19973074.1472923/150‑1 (MQ=60)
                                                                                                 ACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGC                                                     >  SRR19973074.2415058/1‑150 (MQ=60)
                                                                                                  CAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCG                                                    <  SRR19973074.639714/150‑1 (MQ=60)
                                                                                                   AAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGG                                                   >  SRR19973074.458818/1‑150 (MQ=60)
                                                                                                   AAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGG                                                   >  SRR19973074.1073581/1‑150 (MQ=60)
                                                                                                     GTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCT                                                 >  SRR19973074.2015453/1‑150 (MQ=60)
                                                                                                       TACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCC                                               >  SRR19973074.2991407/1‑150 (MQ=60)
                                                                                                         CACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTG                                             >  SRR19973074.2825632/1‑150 (MQ=60)
                                                                                                         CACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACAACAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAATGAGCCGCGGCTCCTG                                             >  SRR19973074.545866/1‑150 (MQ=60)
                                                                                                          ACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAACCACGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGG                                            <  SRR19973074.335545/150‑1 (MQ=60)
                                                                                                            AACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA                                          <  SRR19973074.1262596/150‑1 (MQ=60)
                                                                                                             ACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAA                                         >  SRR19973074.3542306/1‑150 (MQ=60)
                                                                                                             ACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAA                                         >  SRR19973074.3264934/1‑150 (MQ=60)
                                                                                                                 ATTCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATG                                     >  SRR19973074.1254406/1‑150 (MQ=60)
                                                                                                                   TCAGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAA                                   >  SRR19973074.1344612/1‑150 (MQ=60)
                                                                                                                     AGACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAATGAGCCGCGGCTCCTGGTAACATGAAAT                                 >  SRR19973074.331982/1‑150 (MQ=60)
                                                                                                                      GACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGC                                >  SRR19973074.259277/1‑150 (MQ=60)
                                                                                                                      GACTCCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGC                                >  SRR19973074.2626013/1‑150 (MQ=60)
                                                                                                                          CCGCCAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCAC                            >  SRR19973074.2678611/1‑150 (MQ=60)
                                                                                                                              CAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAT                        >  SRR19973074.2797438/1‑150 (MQ=60)
                                                                                                                              CAGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGAT                        <  SRR19973074.2867577/150‑1 (MQ=60)
                                                                                                                               AGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATG                       >  SRR19973074.1171931/1‑150 (MQ=60)
                                                                                                                               AGCCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATG                       >  SRR19973074.2574603/1‑150 (MQ=60)
                                                                                                                                 CCCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTG                     <  SRR19973074.2897948/150‑1 (MQ=60)
                                                                                                                                  CCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGG                    <  SRR19973074.3180753/150‑1 (MQ=60)
                                                                                                                                  CCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGG                    <  SRR19973074.1085970/150‑1 (MQ=60)
                                                                                                                                  CCACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACAT‑AAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGG                   <  SRR19973074.2248576/150‑1 (MQ=60)
                                                                                                                                    ACCAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGC                  <  SRR19973074.4060916/150‑1 (MQ=60)
                                                                                                                                      CAATCATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTT                <  SRR19973074.343034/150‑1 (MQ=60)
                                                                                                                                          CATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTC            >  SRR19973074.3334370/1‑150 (MQ=60)
                                                                                                                                          CATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTC            >  SRR19973074.723428/1‑150 (MQ=60)
                                                                                                                                           ATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGTAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCG           <  SRR19973074.1125021/150‑1 (MQ=60)
                                                                                                                                           ATGATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCG           <  SRR19973074.1125015/150‑1 (MQ=60)
                                                                                                                                             GATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTT         >  SRR19973074.564683/1‑150 (MQ=60)
                                                                                                                                             GATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTACTGGTAACATGAAAGCCCACAGATTTGGGCTTTTTAGTT         >  SRR19973074.784988/1‑150 (MQ=60)
                                                                                                                                              ATTGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTG        <  SRR19973074.2567668/150‑1 (MQ=60)
                                                                                                                                                TGGACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGAT      >  SRR19973074.771269/1‑150 (MQ=60)
                                                                                                                                                   ACAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGT   <  SRR19973074.3002165/150‑1 (MQ=60)
                                                                                                                                                    CAGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTC  >  SRR19973074.1556289/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTC  >  CP009273/2771209‑2771506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑