Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,132,601 T→G 38.3% I172M (ATT→ATG)  yghU → putative S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,132,6010TG38.3% 117.0 / inf 256I172M (ATT→ATG) yghUputative S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (77/81);  new base G (43/55);  total (120/136)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 256 total aligned reads displayed.

TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTT  >  CP009273/3132454‑3132747
                                                                                                                                                   |                                                                                                                                                  
 tATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAg                                                                                                                                                 <  2:2683617/150‑1 (MQ=255)
   tCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTa                                                                                                                                               >  1:2000201/1‑150 (MQ=255)
          gagaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATc                                                                                                                                        <  1:988802/150‑1 (MQ=255)
           agaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCa                                                                                                                                       <  1:519517/150‑1 (MQ=255)
            gaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCaa                                                                                                                                      <  2:2910552/150‑1 (MQ=255)
                 ttGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCt                                                                                                                                 <  2:3041606/150‑1 (MQ=255)
                   ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCttt                                                                                                                               <  2:2574880/150‑1 (MQ=255)
                   ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCttt                                                                                                                               <  1:2270531/150‑1 (MQ=255)
                   ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCttt                                                                                                                               <  2:1358909/150‑1 (MQ=255)
                    gCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTa                                                                                                                              >  1:4164726/1‑150 (MQ=255)
                      tACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                            <  2:2614339/150‑1 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCAt                                                                                                                          >  1:2102298/1‑150 (MQ=255)
                         ttCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATg                                                                                                                         >  1:2559896/1‑150 (MQ=255)
                         ttCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATg                                                                                                                         >  2:1268599/1‑150 (MQ=255)
                            cTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGaa                                                                                                                      <  2:3679992/150‑1 (MQ=255)
                               ccGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGcc                                                                                                                   >  1:572239/1‑150 (MQ=255)
                                cGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                  >  2:1777087/1‑150 (MQ=255)
                                cGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                  <  1:1407222/150‑1 (MQ=255)
                                  cAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaaa                                                                                                                >  2:1452323/1‑150 (MQ=255)
                                  cAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaaa                                                                                                                >  2:561778/1‑150 (MQ=255)
                                   aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAAc                                                                                                               >  2:2670546/1‑150 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACg                                                                                                              <  1:2267973/150‑1 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACg                                                                                                              >  1:3673268/1‑150 (MQ=255)
                                     gATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGt                                                                                                             >  2:157142/1‑150 (MQ=255)
                                      aTTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGGTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTc                                                                                                            >  2:3574192/1‑150 (MQ=255)
                                           gCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTc                                                                                                       <  2:3117714/150‑1 (MQ=255)
                                             aaaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGa                                                                                                     <  1:3087537/150‑1 (MQ=255)
                                              aaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGAc                                                                                                    >  1:900097/1‑150 (MQ=255)
                                              aaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGAc                                                                                                    <  2:895824/150‑1 (MQ=255)
                                              aaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGAc                                                                                                    <  2:4001198/150‑1 (MQ=255)
                                              aaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGAc                                                                                                    >  2:1520506/1‑150 (MQ=255)
                                               aGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACg                                                                                                   <  2:2510155/150‑1 (MQ=255)
                                                 cGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTg                                                                                                 >  1:2864067/1‑150 (MQ=255)
                                                  gTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGc                                                                                                >  2:2684556/1‑150 (MQ=255)
                                                   tACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCt                                                                                               <  2:2561690/150‑1 (MQ=255)
                                                   tACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCt                                                                                               <  2:1916979/150‑1 (MQ=255)
                                                       gAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGAt                                                                                           >  2:2857306/1‑150 (MQ=255)
                                                          aCGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAg                                                                                        <  2:2056239/150‑1 (MQ=255)
                                                          aCGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAg                                                                                        <  2:1415020/150‑1 (MQ=255)
                                                            gATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCa                                                                                      >  2:323383/1‑150 (MQ=255)
                                                              tGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAAc                                                                                    >  2:745487/1‑150 (MQ=255)
                                                               gAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                   >  2:2200999/1‑150 (MQ=255)
                                                                     gCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgca                                                                             >  1:3308261/1‑150 (MQ=255)
                                                                      cTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcag                                                                            <  2:2086179/150‑1 (MQ=255)
                                                                       tGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcagc                                                                           <  1:2643427/150‑1 (MQ=255)
                                                                        gTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcagca                                                                          >  1:2818551/1‑150 (MQ=255)
                                                                         ttCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCAt                                                                         <  1:3483628/150‑1 (MQ=255)
                                                                           cTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATaa                                                                       <  2:3195500/150‑1 (MQ=255)
                                                                            tGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAg                                                                      >  2:1865908/1‑150 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                    <  1:233878/150‑1 (MQ=255)
                                                                                  cAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                >  1:3238631/1‑150 (MQ=255)
                                                                                  cAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                <  1:879771/150‑1 (MQ=255)
                                                                                   aGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgttg                                                               <  2:2077678/150‑1 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGc                                                              <  2:1130925/150‑1 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGc                                                              >  2:2467105/1‑150 (MQ=255)
                                                                                     ggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCg                                                             <  2:1899259/150‑1 (MQ=255)
                                                                                       cgcgGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATGAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCggg                                                           <  1:201786/150‑1 (MQ=255)
                                                                                        gcgGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGc                                                          <  1:3629496/150‑1 (MQ=255)
                                                                                          ggCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                        >  1:709102/1‑150 (MQ=255)
                                                                                          ggCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                        >  1:2832985/1‑150 (MQ=255)
                                                                                          ggCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                        >  1:3370660/1‑150 (MQ=255)
                                                                                          ggCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                        >  2:818648/1‑150 (MQ=255)
                                                                                           gCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGAt                                                       <  1:3687777/150‑1 (MQ=255)
                                                                                            cACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATg                                                      <  1:704040/150‑1 (MQ=255)
                                                                                             aCCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGa                                                     <  1:2774382/150‑1 (MQ=255)
                                                                                             aCCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGAGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGa                                                     >  2:3356532/1‑150 (MQ=255)
                                                                                                gTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTa                                                  >  1:1755658/1‑150 (MQ=255)
                                                                                                   ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTacac                                               >  2:3950753/1‑150 (MQ=255)
                                                                                                   ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTacac                                               <  1:2882094/150‑1 (MQ=255)
                                                                                                      cggcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAt                                            >  2:3840166/1‑150 (MQ=255)
                                                                                                       ggcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAtt                                           >  1:1233948/1‑150 (MQ=255)
                                                                                                       ggcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAtt                                           >  1:1405915/1‑150 (MQ=255)
                                                                                                         cggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGc                                         >  1:3996283/1‑150 (MQ=255)
                                                                                                          ggtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCg                                        <  1:3936606/150‑1 (MQ=255)
                                                                                                          ggtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCg                                        >  2:3277634/1‑150 (MQ=255)
                                                                                                            tggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGa                                      <  1:3753382/150‑1 (MQ=255)
                                                                                                             ggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGat                                     <  1:442355/150‑1 (MQ=255)
                                                                                                              gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                    >  1:1549332/1‑150 (MQ=255)
                                                                                                              gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                    <  1:3311789/150‑1 (MQ=255)
                                                                                                              gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                    >  1:820424/1‑150 (MQ=255)
                                                                                                                  tGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGc                                <  1:1051369/150‑1 (MQ=255)
                                                                                                                    gTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGa                              >  1:4062356/1‑150 (MQ=255)
                                                                                                                    gTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGa                              >  1:165490/1‑150 (MQ=255)
                                                                                                                     tCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAt                             >  2:4144000/1‑150 (MQ=255)
                                                                                                                      cACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAtt                            >  1:3816758/1‑150 (MQ=255)
                                                                                                                         ttttACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCAGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTgg                         >  2:2376063/1‑150 (MQ=255)
                                                                                                                            tACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCg                      <  2:2947464/150‑1 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTg                    >  1:3198815/1‑150 (MQ=255)
                                                                                                                                  tACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGttt                <  1:407253/150‑1 (MQ=255)
                                                                                                                                  tACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGttt                <  2:1097421/150‑1 (MQ=255)
                                                                                                                                   aCGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTg               <  1:1290096/150‑1 (MQ=255)
                                                                                                                                   aCGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTg               <  2:1998406/150‑1 (MQ=255)
                                                                                                                                         cGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACg         >  1:2912277/1‑150 (MQ=255)
                                                                                                                                         cGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACg         >  1:301328/1‑150 (MQ=255)
                                                                                                                                           gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg       <  2:2753133/150‑1 (MQ=255)
                                                                                                                                           gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg       >  2:1791202/1‑150 (MQ=255)
                                                                                                                                           gTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg       >  1:1137956/1‑150 (MQ=255)
                                                                                                                                             aaaGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtggt     >  1:3760306/1‑150 (MQ=255)
                                                                                                                                                gATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGtt  <  1:1800258/150‑1 (MQ=255)
                                                                                                                                                gATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGtt  >  1:1472167/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTT  >  CP009273/3132454‑3132747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A