Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,266,646 C→A 100% G91C (GGT→TGT)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,266,6460CA98.4% 860.1 / 3.9 246G91C (GGT→TGT) garLalpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (127/115);  total (127/119)
Fisher's exact test for biased strand distribution p-value = 5.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

CGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266499‑3266792
                                                                                                                                                   |                                                                                                                                                  
cGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGa                                                                                                                                                  <  2:284136/150‑1 (MQ=255)
cGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGa                                                                                                                                                  >  2:152899/1‑150 (MQ=255)
 gAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGat                                                                                                                                                 <  1:2344069/150‑1 (MQ=255)
  aaaTAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGata                                                                                                                                                >  1:1576642/1‑150 (MQ=255)
   aataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                               <  1:768868/150‑1 (MQ=255)
     taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATcc                                                                                                                                             <  1:3420211/150‑1 (MQ=255)
      aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            >  1:1775169/1‑150 (MQ=255)
         ccGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCagaa                                                                                                                                         <  1:440352/150‑1 (MQ=255)
          cGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCagaag                                                                                                                                        <  2:3183353/150‑1 (MQ=255)
             cACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACg                                                                                                                                     >  2:2423762/1‑150 (MQ=255)
                ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCtt                                                                                                                                  <  1:1235919/150‑1 (MQ=255)
                  tGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaa                                                                                                                                <  2:3541899/150‑1 (MQ=255)
                   gCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaat                                                                                                                               <  2:2566246/150‑1 (MQ=255)
                   gCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaat                                                                                                                               <  2:797150/150‑1 (MQ=255)
                        aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                          >  2:1488043/1‑150 (MQ=255)
                        aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                          >  1:2384777/1‑150 (MQ=255)
                         cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                         <  2:4087101/150‑1 (MQ=255)
                         cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                         >  2:958801/1‑150 (MQ=255)
                          atatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAcc                                                                                                                        <  2:1135223/150‑1 (MQ=255)
                          atatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAcc                                                                                                                        >  1:914183/1‑150 (MQ=255)
                           tatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCg                                                                                                                       <  1:626135/150‑1 (MQ=255)
                              tGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCAAGAAGACGCTTAATAATTACCGGCt                                                                                                                    >  2:227475/1‑150 (MQ=255)
                               ggCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTc                                                                                                                   <  2:867346/150‑1 (MQ=255)
                               ggCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTc                                                                                                                   >  2:3604128/1‑150 (MQ=255)
                                 cgcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGt                                                                                                                 <  2:2108344/150‑1 (MQ=255)
                                  gcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGtt                                                                                                                <  1:2603032/150‑1 (MQ=255)
                                    ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              >  1:3128175/1‑150 (MQ=255)
                                    ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              >  2:3472465/1‑150 (MQ=255)
                                    ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              <  2:2651245/150‑1 (MQ=255)
                                    ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              <  2:2675043/150‑1 (MQ=255)
                                     gTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGt                                                                                                             <  2:825902/150‑1 (MQ=255)
                                     gTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGt                                                                                                             <  1:2039035/150‑1 (MQ=255)
                                       gagaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTCTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCg                                                                                                           >  2:2311598/1‑150 (MQ=255)
                                         gaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGc                                                                                                         <  1:1492628/150‑1 (MQ=255)
                                              ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  2:1676790/1‑150 (MQ=255)
                                              ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  2:608611/1‑150 (MQ=255)
                                              ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  1:515208/1‑150 (MQ=255)
                                              ggAGACGCCGCGAATGACAACCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTAATAGAAACAGATATCCAGAAGACGCTTAATAAGTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  2:3954444/1‑150 (MQ=255)
                                                agaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCa                                                                                                  >  1:2230264/1‑150 (MQ=255)
                                                 gaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAACCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCac                                                                                                 >  2:3862348/1‑150 (MQ=255)
                                                  aCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCact                                                                                                <  1:3691177/150‑1 (MQ=255)
                                                   cgccgcGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCacta                                                                                               <  1:2773374/150‑1 (MQ=255)
                                                    gccgcGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCactac                                                                                              >  1:1353756/1‑150 (MQ=255)
                                                      cgcgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTg                                                                                            <  1:146432/150‑1 (MQ=255)
                                                      cgcgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTg                                                                                            <  1:1875460/150‑1 (MQ=255)
                                                        cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          <  1:245048/150‑1 (MQ=255)
                                                         gAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcg                                                                                         >  1:1819518/1‑150 (MQ=255)
                                                             gCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgct                                                                                     <  2:3906561/150‑1 (MQ=255)
                                                              ccTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctg                                                                                    >  2:483541/1‑150 (MQ=255)
                                                              ccTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctg                                                                                    <  2:756327/150‑1 (MQ=255)
                                                               cTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctgg                                                                                   >  2:3103659/1‑150 (MQ=255)
                                                               cTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctgg                                                                                   >  2:670933/1‑150 (MQ=255)
                                                                 tCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcg                                                                                 <  2:412495/150‑1 (MQ=255)
                                                                   cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAATTTATAGAAACAGATATACAGAAGACGCTTAAAAATTACCGGCTCGTTGGGCGGCACTCGCACTACTGTCgcgctggcgct                                                                               >  1:1612999/1‑150 (MQ=255)
                                                                   cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgct                                                                               <  1:2318281/150‑1 (MQ=255)
                                                                    ggTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgctg                                                                              >  2:3259853/1‑150 (MQ=255)
                                                                      tGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGcc                                                                            <  2:535637/150‑1 (MQ=255)
                                                                          tAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTc                                                                        <  1:333832/150‑1 (MQ=255)
                                                                              gggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAgg                                                                    >  1:1028030/1‑150 (MQ=255)
                                                                               ggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGc                                                                   >  2:3812076/1‑150 (MQ=255)
                                                                                 ttGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCa                                                                 <  1:2480805/150‑1 (MQ=255)
                                                                                  tGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCAt                                                                >  1:3230571/1‑150 (MQ=255)
                                                                                  tGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTGATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCAt                                                                >  2:3011157/1‑150 (MQ=255)
                                                                                      gCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAAc                                                            >  2:1280664/1‑150 (MQ=255)
                                                                                      gCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAAc                                                            >  1:4044212/1‑150 (MQ=255)
                                                                                       ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACt                                                           <  1:2488503/150‑1 (MQ=255)
                                                                                        cACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTg                                                          >  2:1019022/1‑150 (MQ=255)
                                                                                        cACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTg                                                          <  1:3943602/150‑1 (MQ=255)
                                                                                        cACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATACAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTg                                                          >  2:1943061/1‑150 (MQ=255)
                                                                                         aCCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGc                                                         >  2:1952729/1‑150 (MQ=255)
                                                                                          ccGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCg                                                        <  2:3415508/150‑1 (MQ=255)
                                                                                          ccGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCg                                                        <  2:3415480/150‑1 (MQ=255)
                                                                                          ccGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCg                                                        <  2:488154/150‑1 (MQ=255)
                                                                                            gCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                      <  1:1055044/150‑1 (MQ=255)
                                                                                            gCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                      >  1:3245514/1‑150 (MQ=255)
                                                                                              cAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAAt                                                    >  2:2998765/1‑150 (MQ=255)
                                                                                               aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATa                                                   >  2:752982/1‑150 (MQ=255)
                                                                                                gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaa                                                  >  1:3834627/1‑150 (MQ=255)
                                                                                                gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaa                                                  >  1:3834635/1‑150 (MQ=255)
                                                                                                   ctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACg                                               >  2:2528699/1‑150 (MQ=255)
                                                                                                       ttCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGga                                           >  1:1624159/1‑150 (MQ=255)
                                                                                                       ttCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGga                                           <  1:846754/150‑1 (MQ=255)
                                                                                                          ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGat                                        <  2:3050227/150‑1 (MQ=255)
                                                                                                           tctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGata                                       <  1:3284658/150‑1 (MQ=255)
                                                                                                            ctTTTGTTTCTACAAAAGGAATCAGGTAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGatat                                      <  2:1565008/150‑1 (MQ=255)
                                                                                                             ttttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATc                                     >  1:1334547/1‑150 (MQ=255)
                                                                                                             ttttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATc                                     <  2:4073230/150‑1 (MQ=255)
                                                                                                                 gTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGttt                                 <  1:3741342/150‑1 (MQ=255)
                                                                                                                   ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTgg                               <  1:1618402/150‑1 (MQ=255)
                                                                                                                   ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTgg                               <  2:1858761/150‑1 (MQ=255)
                                                                                                                       aCAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCa                           >  1:4077511/1‑150 (MQ=255)
                                                                                                                               aaTCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGcc                   >  2:2455843/1‑150 (MQ=255)
                                                                                                                                  cAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATc                <  2:294208/150‑1 (MQ=255)
                                                                                                                                     gAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAg             <  1:3515669/150‑1 (MQ=255)
                                                                                                                                       aGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCa           <  2:2215467/150‑1 (MQ=255)
                                                                                                                                            taGAAACAGATATCCAGAAGACGCTTAATAATTACCGGATCGTTGGTCGGCACTCGCACTACTGTCGCGCTGGCGCTGACTTTCAAGGCCATTAACTGCGGAATAAACGTGGATATATCGTTTGGCGAATGTTCGCCATCCAGCAccagc      >  2:2479930/1‑150 (MQ=255)
                                                                                                                                              gAAACAGATATCGAGAAGACTCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCTCTGGCGCTGCCTTTCTAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGAAccagcca    <  2:3208037/150‑1 (MQ=255)
                                                                                                                                              gAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagcca    >  2:527919/1‑150 (MQ=255)
                                                                                                                                               aaaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagccag   >  2:35132/1‑150 (MQ=255)
                                                                                                                                                aaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCAATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGt  >  2:1414934/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266499‑3266792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGTCAA  >  CP009273/3266497‑3266795
                                                                                                                                                     |                                                                                                                                                     
AGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACA                                                                                                                                                       >  SRR19973074.283927/1‑150 (MQ=60)
 GCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAG                                                                                                                                                      <  SRR19973074.3123150/150‑1 (MQ=60)
 GCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAG                                                                                                                                                      <  SRR19973074.3313379/150‑1 (MQ=60)
  CGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGA                                                                                                                                                     >  SRR19973074.38231/1‑150 (MQ=60)
  CGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGA                                                                                                                                                     >  SRR19973074.2382598/1‑150 (MQ=60)
   GAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGAT                                                                                                                                                    <  SRR19973074.2348448/150‑1 (MQ=60)
    AAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATA                                                                                                                                                   >  SRR19973074.1579640/1‑150 (MQ=60)
     AATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATAT                                                                                                                                                  <  SRR19973074.770344/150‑1 (MQ=60)
       TAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCC                                                                                                                                                <  SRR19973074.736718/150‑1 (MQ=60)
       TAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCC                                                                                                                                                <  SRR19973074.3426526/150‑1 (MQ=60)
        AATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCA                                                                                                                                               >  SRR19973074.1778513/1‑150 (MQ=60)
           CCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAA                                                                                                                                            <  SRR19973074.441203/150‑1 (MQ=60)
             GCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGA                                                                                                                                          <  SRR19973074.445487/150‑1 (MQ=60)
                  GGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTT                                                                                                                                     <  SRR19973074.1238285/150‑1 (MQ=60)
                  GGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTT                                                                                                                                     >  SRR19973074.844898/1‑150 (MQ=60)
                   GTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTA                                                                                                                                    <  SRR19973074.2569077/150‑1 (MQ=60)
                    TGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGCAGACGCTTAA                                                                                                                                   <  SRR19973074.1840755/150‑1 (MQ=60)
                     GCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAAT                                                                                                                                  <  SRR19973074.3822727/150‑1 (MQ=60)
                         AACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATT                                                                                                                              <  SRR19973074.2518114/150‑1 (MQ=60)
                          ACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTA                                                                                                                             >  SRR19973074.2389203/1‑150 (MQ=60)
                          ACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTA                                                                                                                             >  SRR19973074.2389144/1‑150 (MQ=60)
                            ATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACC                                                                                                                           >  SRR19973074.915927/1‑150 (MQ=60)
                            ATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACC                                                                                                                           >  SRR19973074.2285789/1‑150 (MQ=60)
                             TATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCG                                                                                                                          >  SRR19973074.3260844/1‑150 (MQ=60)
                             TATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCG                                                                                                                          <  SRR19973074.627312/150‑1 (MQ=60)
                                    GCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTT                                                                                                                   <  SRR19973074.2607900/150‑1 (MQ=60)
                                      GGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGG                                                                                                                 >  SRR19973074.3133896/1‑150 (MQ=60)
                                      GGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGG                                                                                                                 >  SRR19973074.3133904/1‑150 (MQ=60)
                                       GTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGT                                                                                                                <  SRR19973074.2042878/150‑1 (MQ=60)
                                         GAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTACTACAAAAGGAATCAGGAAGTTATAGAAACAGAAATCCAGAAGACGCTTAAAAATTACCGGCTCGTTGGTAG                                                                                                              >  SRR19973074.1996230/1‑150 (MQ=60)
                                           GAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGC                                                                                                            <  SRR19973074.1495438/150‑1 (MQ=60)
                                               CGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTC                                                                                                        <  SRR19973074.600936/150‑1 (MQ=60)
                                                GGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCG                                                                                                       >  SRR19973074.1685351/1‑150 (MQ=60)
                                                GGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCG                                                                                                       >  SRR19973074.516238/1‑150 (MQ=60)
                                                  AGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCA                                                                                                     >  SRR19973074.2234459/1‑150 (MQ=60)
                                                    ACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACT                                                                                                   <  SRR19973074.3742528/150‑1 (MQ=60)
                                                    ACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACT                                                                                                   <  SRR19973074.3698002/150‑1 (MQ=60)
                                                    ACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACT                                                                                                   <  SRR19973074.3697957/150‑1 (MQ=60)
                                                     CGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTA                                                                                                  <  SRR19973074.2778465/150‑1 (MQ=60)
                                                     CGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTA                                                                                                  <  SRR19973074.2778448/150‑1 (MQ=60)
                                                      GCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTAC                                                                                                 >  SRR19973074.1356352/1‑150 (MQ=60)
                                                      GCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTAC                                                                                                 >  SRR19973074.1356259/1‑150 (MQ=60)
                                                      GCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTAC                                                                                                 >  SRR19973074.1356274/1‑150 (MQ=60)
                                                        CGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTG                                                                                               <  SRR19973074.146762/150‑1 (MQ=60)
                                                        CGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTG                                                                                               <  SRR19973074.1879048/150‑1 (MQ=60)
                                                          CGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGC                                                                                             <  SRR19973074.245537/150‑1 (MQ=60)
                                                          CGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGC                                                                                             <  SRR19973074.245556/150‑1 (MQ=60)
                                                           GAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCG                                                                                            >  SRR19973074.1822984/1‑150 (MQ=60)
                                                             ATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCG                                                                                          <  SRR19973074.1398303/150‑1 (MQ=60)
                                                              TGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGC                                                                                         >  SRR19973074.861556/1‑150 (MQ=60)
                                                               GCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCT                                                                                        <  SRR19973074.1306800/150‑1 (MQ=60)
                                                                CCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTG                                                                                       <  SRR19973074.4056606/150‑1 (MQ=60)
                                                                     CGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAATTTATAGAAACAGATATACAGAAGACGCTTAAAAATTACCGGCTCGTTGGGCGGCACTCGCACTACTGTCGCGCTGGCGCT                                                                                  >  SRR19973074.1616064/1‑150 (MQ=60)
                                                                     CGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCT                                                                                  <  SRR19973074.2322614/150‑1 (MQ=60)
                                                                     CGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCT                                                                                  <  SRR19973074.3885914/150‑1 (MQ=60)
                                                                          GGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTT                                                                             >  SRR19973074.3388887/1‑150 (MQ=60)
                                                                            TAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTC                                                                           <  SRR19973074.334415/150‑1 (MQ=60)
                                                                            TAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTC                                                                           <  SRR19973074.334441/150‑1 (MQ=60)
                                                                                GGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGG                                                                       >  SRR19973074.1029932/1‑150 (MQ=60)
                                                                                GGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGG                                                                       >  SRR19973074.1029927/1‑150 (MQ=60)
                                                                                   TTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCA                                                                    <  SRR19973074.2485419/150‑1 (MQ=60)
                                                                                   TTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCA                                                                    <  SRR19973074.2485404/150‑1 (MQ=60)
                                                                                    TGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCAT                                                                   >  SRR19973074.3236546/1‑150 (MQ=60)
                                                                                       TGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAA                                                                >  SRR19973074.359661/1‑150 (MQ=60)
                                                                                        GCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAAC                                                               >  SRR19973074.4051691/1‑150 (MQ=60)
                                                                                         CCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACT                                                              <  SRR19973074.2493123/150‑1 (MQ=60)
                                                                                          CACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTG                                                             <  SRR19973074.3950869/150‑1 (MQ=60)
                                                                                              GCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGA                                                         >  SRR19973074.3251521/1‑150 (MQ=60)
                                                                                              GCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGA                                                         <  SRR19973074.4041669/150‑1 (MQ=60)
                                                                                              GCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGA                                                         >  SRR19973074.3251180/1‑150 (MQ=60)
                                                                                              GCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGA                                                         <  SRR19973074.1056987/150‑1 (MQ=60)
                                                                                               CCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAA                                                        >  SRR19973074.1610186/1‑150 (MQ=60)
                                                                                                  GCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAA                                                     >  SRR19973074.3841621/1‑150 (MQ=60)
                                                                                                  GCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAA                                                     >  SRR19973074.3841617/1‑150 (MQ=60)
                                                                                                  GCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAA                                                     >  SRR19973074.3841625/1‑150 (MQ=60)
                                                                                                     CTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACG                                                  >  SRR19973074.2133992/1‑150 (MQ=60)
                                                                                                       GCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTG                                                <  SRR19973074.1747182/150‑1 (MQ=60)
                                                                                                        CTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGG                                               >  SRR19973074.2792694/1‑150 (MQ=60)
                                                                                                         TTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGA                                              <  SRR19973074.848386/150‑1 (MQ=60)
                                                                                                         TTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGA                                              >  SRR19973074.1627240/1‑150 (MQ=60)
                                                                                                         TTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGA                                              >  SRR19973074.1627207/1‑150 (MQ=60)
                                                                                                          TCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAG                                             <  SRR19973074.1821088/150‑1 (MQ=60)
                                                                                                           CCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGA                                            <  SRR19973074.785867/150‑1 (MQ=60)
                                                                                                           CCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGA                                            >  SRR19973074.954137/1‑150 (MQ=60)
                                                                                                             TCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATA                                          <  SRR19973074.1563229/150‑1 (MQ=60)
                                                                                                             TCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATA                                          <  SRR19973074.3290755/150‑1 (MQ=60)
                                                                                                             TCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATA                                          <  SRR19973074.3290745/150‑1 (MQ=60)
                                                                                                              CTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATAT                                         <  SRR19973074.2479584/150‑1 (MQ=60)
                                                                                                               TTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATC                                        >  SRR19973074.1337040/1‑150 (MQ=60)
                                                                                                                  TGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTT                                     >  SRR19973074.555522/1‑150 (MQ=60)
                                                                                                                   GTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTT                                    <  SRR19973074.1562206/150‑1 (MQ=60)
                                                                                                                   GTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTT                                    <  SRR19973074.3748228/150‑1 (MQ=60)
                                                                                                                     TTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGG                                  <  SRR19973074.1621475/150‑1 (MQ=60)
                                                                                                                     TTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGG                                  <  SRR19973074.1621047/150‑1 (MQ=60)
                                                                                                                     TTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGG                                  >  SRR19973074.1471016/1‑150 (MQ=60)
                                                                                                                      TCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGC                                 <  SRR19973074.1619289/150‑1 (MQ=60)
                                                                                                                       CTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCG                                >  SRR19973074.3875753/1‑150 (MQ=60)
                                                                                                                        TACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGC                               >  SRR19973074.2399418/1‑150 (MQ=60)
                                                                                                                         ACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCA                              >  SRR19973074.4085047/1‑150 (MQ=60)
                                                                                                                         ACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCA                              >  SRR19973074.4085053/1‑150 (MQ=60)
                                                                                                                           AAAAGGAATCATGAAGTTATAGAAACAGATATCCAGAAGACGATTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATG                            <  SRR19973074.2332634/150‑1 (MQ=60)
                                                                                                                           AAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATG                            >  SRR19973074.1999168/1‑150 (MQ=60)
                                                                                                                                   TCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCAT                    >  SRR19973074.2247515/1‑150 (MQ=60)
                                                                                                                                      GGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCA                 <  SRR19973074.965495/150‑1 (MQ=60)
                                                                                                                                       GAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAG                <  SRR19973074.3522129/150‑1 (MQ=60)
                                                                                                                                       GAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAG                <  SRR19973074.3522128/150‑1 (MQ=60)
                                                                                                                                         AGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCA              <  SRR19973074.1334462/150‑1 (MQ=60)
                                                                                                                                             ATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAG          >  SRR19973074.4131103/1‑150 (MQ=60)
                                                                                                                                             ATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAG          >  SRR19973074.3385923/1‑150 (MQ=60)
                                                                                                                                                GAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCA       >  SRR19973074.1135137/1‑150 (MQ=60)
                                                                                                                                                  AACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT     >  SRR19973074.3188992/1‑150 (MQ=60)
                                                                                                                                                    CAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGTCA   >  SRR19973074.3665069/1‑150 (MQ=60)
                                                                                                                                                    CAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGTCA   >  SRR19973074.3664926/1‑150 (MQ=60)
                                                                                                                                                     AGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGTCAA  >  SRR19973074.2728662/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGTCAA  >  CP009273/3266497‑3266795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑