Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,510,311 C→A 37.7% G119C (GGT→TGT)  damX ← cell division protein that binds to the septal ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,510,3110CA37.7% 382.9 / 284.2 272G119C (GGT→TGT) damXcell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base C (87/82);  new base A (48/55);  total (135/137)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

CTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTT  >  CP009273/3510162‑3510457
                                                                                                                                                     |                                                                                                                                                  
ctgAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgatacc                                                                                                                                                    <  1:4122945/150‑1 (MQ=255)
   aGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgatacagat                                                                                                                                                 <  2:891897/150‑1 (MQ=255)
       ttttCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATc                                                                                                                                             >  2:2557631/1‑150 (MQ=255)
       ttttCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATc                                                                                                                                             <  1:3713484/150‑1 (MQ=255)
           ccGCAGACGTGGGTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAAca                                                                                                                                         <  1:1508443/150‑1 (MQ=255)
             gCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAAcagc                                                                                                                                       >  2:2273028/1‑150 (MQ=255)
              cAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAAcagca                                                                                                                                      >  2:2211100/1‑150 (MQ=255)
               aGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAAcagcag                                                                                                                                     >  1:2716775/1‑150 (MQ=255)
               aGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTATTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAAcagcag                                                                                                                                     >  2:2139315/1‑150 (MQ=255)
                gACGTGGTTCCCGGCGCTGGCTGCTCGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGt                                                                                                                                    <  2:4027675/150‑1 (MQ=255)
                gACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGTTCGGTCGCTTTGCCAGCATGATCATTACTCTTCTCGCCAGACGCGGTTTGATCGCTGGATGTGGTCGAGGGGGCTTTTAGCGCAGAAGCGATACAGATGATCATCTGCAGt                                                                                                                                    <  2:4055226/150‑1 (MQ=255)
                  cGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAg                                                                                                                                  >  2:2935777/1‑150 (MQ=255)
                   gTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGa                                                                                                                                 >  2:1166312/1‑150 (MQ=255)
                   gTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGa                                                                                                                                 >  2:833882/1‑150 (MQ=255)
                   gTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGa                                                                                                                                 >  1:705680/1‑150 (MQ=255)
                     ggTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAAc                                                                                                                               >  2:3135863/1‑150 (MQ=255)
                        tCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAg                                                                                                                            <  1:632803/150‑1 (MQ=255)
                        tCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAg                                                                                                                            >  1:2985731/1‑150 (MQ=255)
                           cGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAAt                                                                                                                         <  1:3254413/150‑1 (MQ=255)
                           cGGCGCTGGCTGCACGCCAGTCGCCTGATCGGTCGGATTGCCAGCAAGATCTATTCTCTTCTCGCCAGTCGCGTTTTGATGGATGGTAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACTGCAGTTGAACCAGAAt                                                                                                                         <  2:1218462/150‑1 (MQ=255)
                            ggCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATg                                                                                                                        <  1:2473799/150‑1 (MQ=255)
                              cgcTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGcc                                                                                                                      >  2:1801220/1‑150 (MQ=255)
                               gctggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCg                                                                                                                     <  2:2734808/150‑1 (MQ=255)
                               gctggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCg                                                                                                                     <  2:3924359/150‑1 (MQ=255)
                               gctggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCg                                                                                                                     >  2:634391/1‑150 (MQ=255)
                                ctggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGa                                                                                                                    >  1:1239737/1‑150 (MQ=255)
                                ctggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGa                                                                                                                    <  2:3811223/150‑1 (MQ=255)
                                 tggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGAc                                                                                                                   >  2:1122986/1‑150 (MQ=255)
                                  ggctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACg                                                                                                                  <  1:3594352/150‑1 (MQ=255)
                                     tgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGccc                                                                                                               >  1:811514/1‑150 (MQ=255)
                                     tgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGccc                                                                                                               >  1:1359151/1‑150 (MQ=255)
                                          gCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcat                                                                                                          <  2:2456855/150‑1 (MQ=255)
                                            cATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatca                                                                                                        <  1:84176/150‑1 (MQ=255)
                                            cATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatca                                                                                                        <  1:4071600/150‑1 (MQ=255)
                                             aTTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatcat                                                                                                       <  1:2842738/150‑1 (MQ=255)
                                               tCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCatat                                                                                                     <  1:221167/150‑1 (MQ=255)
                                                  ccTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACt                                                                                                  <  2:1456996/150‑1 (MQ=255)
                                                       tCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACga                                                                                             >  2:3372868/1‑150 (MQ=255)
                                                         ggTCGCATTGCCAGCAAGATCAATACTCTTGTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACgaga                                                                                           >  1:2140034/1‑150 (MQ=255)
                                                           tCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAg                                                                                         >  2:482343/1‑150 (MQ=255)
                                                           tCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAg                                                                                         >  2:3689405/1‑150 (MQ=255)
                                                           tCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAg                                                                                         <  2:1563313/150‑1 (MQ=255)
                                                             gCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCg                                                                                       >  2:734254/1‑150 (MQ=255)
                                                              cATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCgg                                                                                      >  2:1846528/1‑150 (MQ=255)
                                                                ttGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGt                                                                                    <  2:3640404/150‑1 (MQ=255)
                                                                  gCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGttt                                                                                  <  1:1141196/150‑1 (MQ=255)
                                                                    cAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTAc                                                                                >  2:140615/1‑150 (MQ=255)
                                                                      gCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTg                                                                              >  2:1310480/1‑150 (MQ=255)
                                                                        aaGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgc                                                                            >  2:3953567/1‑150 (MQ=255)
                                                                          gATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgctg                                                                          <  1:2733111/150‑1 (MQ=255)
                                                                               aTACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCtttt                                                                     <  2:3549137/150‑1 (MQ=255)
                                                                               aTACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGACGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTTCtttt                                                                     >  1:157327/1‑150 (MQ=255)
                                                                               aTACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCtttt                                                                     >  2:907633/1‑150 (MQ=255)
                                                                                tACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCttttt                                                                    <  2:32796/150‑1 (MQ=255)
                                                                                tACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCttttt                                                                    <  2:2088464/150‑1 (MQ=255)
                                                                                 aCTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCtttttt                                                                   >  2:1271999/1‑150 (MQ=255)
                                                                                  ctctTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTg                                                                  >  2:2886923/1‑150 (MQ=255)
                                                                                        tcGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTa                                                            <  1:448155/150‑1 (MQ=255)
                                                                                         cGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTAc                                                           <  1:363896/150‑1 (MQ=255)
                                                                                         cGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTAc                                                           <  1:2445091/150‑1 (MQ=255)
                                                                                         cGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTAc                                                           <  1:2561979/150‑1 (MQ=255)
                                                                                          gCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTAcg                                                          >  2:2560158/1‑150 (MQ=255)
                                                                                             aGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCgg                                                       >  1:693623/1‑150 (MQ=255)
                                                                                             aGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCgg                                                       <  2:2761858/150‑1 (MQ=255)
                                                                                               aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgac                                                     <  1:343432/150‑1 (MQ=255)
                                                                                               aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgac                                                     <  1:2264343/150‑1 (MQ=255)
                                                                                               aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgac                                                     <  1:841282/150‑1 (MQ=255)
                                                                                                 gcgGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgacga                                                   <  1:1864085/150‑1 (MQ=255)
                                                                                                 gcgGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgacga                                                   <  1:3962662/150‑1 (MQ=255)
                                                                                                  cgGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgacgac                                                  <  2:541662/150‑1 (MQ=255)
                                                                                                   ggTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGacgacg                                                 >  1:978957/1‑150 (MQ=255)
                                                                                                       tGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGctct                                             <  1:1784886/150‑1 (MQ=255)
                                                                                                         aTCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAc                                           <  1:483152/150‑1 (MQ=255)
                                                                                                         aTCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAc                                           >  2:3642177/1‑150 (MQ=255)
                                                                                                         aTCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAc                                           <  1:384274/150‑1 (MQ=255)
                                                                                                            gCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcgcg                                        >  1:2309216/1‑150 (MQ=255)
                                                                                                            gCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcgcg                                        >  1:3382800/1‑150 (MQ=255)
                                                                                                              tGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGct                                      <  2:4114631/150‑1 (MQ=255)
                                                                                                                  aGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTc                                  >  2:3787596/1‑150 (MQ=255)
                                                                                                                     ggTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATc                               >  1:1118560/1‑150 (MQ=255)
                                                                                                                       tCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCa                             <  1:2273028/150‑1 (MQ=255)
                                                                                                                       tCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCa                             >  1:2003898/1‑150 (MQ=255)
                                                                                                                         gAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACTAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACg                           <  2:2216935/150‑1 (MQ=255)
                                                                                                                         gAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACg                           >  2:2646107/1‑150 (MQ=255)
                                                                                                                              ggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                      <  1:3523800/150‑1 (MQ=255)
                                                                                                                              ggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                      <  2:1359958/150‑1 (MQ=255)
                                                                                                                              ggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                      >  2:3093323/1‑150 (MQ=255)
                                                                                                                                cTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAt                    <  1:861715/150‑1 (MQ=255)
                                                                                                                                 ttttAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATc                   >  1:2401454/1‑150 (MQ=255)
                                                                                                                                    tAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTc                <  1:3915622/150‑1 (MQ=255)
                                                                                                                                    tAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTc                <  2:603531/150‑1 (MQ=255)
                                                                                                                                     aGCGCAGATCAGATACCGTTGATCAACAGCATTAGAACCAGAATGCCTACGCCCATCATCATATACTTACGAGAAGCGGTTTTACTGGCTGCTTTTTTGCGCTTACGCGTACGACGCTCTACTCGCTCTTCATCCACGGTTTCATCTTCg               <  2:4165546/150‑1 (MQ=255)
                                                                                                                                     aGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCg               >  1:3982494/1‑150 (MQ=255)
                                                                                                                                         cAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGAtt           >  2:1536050/1‑150 (MQ=255)
                                                                                                                                         cAGAACCGATACAGATGATCAACAGCAGTAGAANCAGAATGCCNACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGAtt           <  1:1384424/150‑1 (MQ=255)
                                                                                                                                           gAACCGATACCGATGATCAACAGCTGTAGAACCAGTTTGCCGACGACCATCATCTTATACTGACGCGATGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCTTCCACGGTTTCATCTTCGGATTCg         <  2:1659495/150‑1 (MQ=255)
                                                                                                                                            aaCCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGt        >  2:2325194/1‑150 (MQ=255)
                                                                                                                                              ccgataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTct      <  2:811572/150‑2 (MQ=255)
                                                                                                                                                 atacagatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAAtt   >  1:2930531/1‑150 (MQ=255)
                                                                                                                                                  taccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAAttt  >  2:2196516/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTT  >  CP009273/3510162‑3510457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A