Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,087,754 C→A 44.4% H31N (CAT→AAT)  rhaS → transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,087,7540CA44.4% 209.8 / inf 245H31N (CAT→AAT) rhaStranscriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base C (60/76);  new base A (51/58);  total (111/134)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 256 total aligned reads displayed.

TAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGT  >  CP009273/4087607‑4087903
                                                                                                                                                   |                                                                                                                                                     
tAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAaat                                                                                                                                                     <  1:1294753/150‑1 (MQ=255)
       cTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATg                                                                                                                                              >  1:1409960/1‑150 (MQ=255)
        tGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGa                                                                                                                                             >  1:435856/1‑150 (MQ=255)
         gACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGAt                                                                                                                                            <  1:1065246/150‑1 (MQ=255)
           ccTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGAttt                                                                                                                                          <  1:2937977/150‑1 (MQ=255)
           ccTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCATATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGAttt                                                                                                                                          <  1:2677476/150‑1 (MQ=255)
            cTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGAtttt                                                                                                                                         <  2:3744729/150‑1 (MQ=255)
             tCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTc                                                                                                                                        <  2:1533736/150‑1 (MQ=255)
                tgatgtcTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATg                                                                                                                                     <  2:3626504/144‑1 (MQ=255)
                tGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATg                                                                                                                                     >  2:629328/1‑150 (MQ=255)
                tGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATg                                                                                                                                     >  1:1813254/1‑150 (MQ=255)
                tGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCATGAACATCATCATGATTTTCATg                                                                                                                                     <  2:726436/150‑1 (MQ=255)
                    tACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCAGTATTGTAACGCGTCCGTGGCGATATAACCCAGGCTCCCGCAGGCGGATTTTCCAGAACATAATCATTATTTTAATGAAAt                                                                                                                                 >  1:725112/1‑150 (MQ=255)
                     aCTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAAtt                                                                                                                                <  2:1599523/150‑1 (MQ=255)
                      cTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATtg                                                                                                                               <  1:1594840/150‑1 (MQ=255)
                      cTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATtg                                                                                                                               <  2:1242856/150‑1 (MQ=255)
                      cTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATtg                                                                                                                               >  1:1204294/1‑150 (MQ=255)
                      cTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATtg                                                                                                                               <  1:942865/150‑1 (MQ=255)
                       tATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATtgt                                                                                                                              <  2:2392871/150‑1 (MQ=255)
                       tATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATtgt                                                                                                                              >  1:787267/1‑150 (MQ=255)
                       tATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATAATCATGATTTTCATGAAATtgt                                                                                                                              >  2:2880821/1‑150 (MQ=255)
                        aTTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATtgtg                                                                                                                             <  1:3044017/150‑1 (MQ=255)
                         tttCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGa                                                                                                                            <  2:419663/150‑1 (MQ=255)
                          ttCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACAGAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAACTCATCATGATTTTCATGAACTTGTGAt                                                                                                                           >  2:1202112/1‑150 (MQ=255)
                            cGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATAATCATGATTTTCATGAAATTGTGATTg                                                                                                                         >  1:853906/1‑150 (MQ=255)
                                  gtTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAAc                                                                                                                   <  2:93543/150‑1 (MQ=255)
                                    tGACGACATCAGGAGGCCAGTATTACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACAt                                                                                                                 <  1:3433905/150‑1 (MQ=255)
                                      acgacATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATgg                                                                                                               <  1:3214138/150‑1 (MQ=255)
                                      acgacATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATgg                                                                                                               >  1:2903316/1‑150 (MQ=255)
                                        gacATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATATAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCa                                                                                                             >  1:1433231/1‑150 (MQ=255)
                                        gacATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCa                                                                                                             <  2:2493869/150‑1 (MQ=255)
                                        gacATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCa                                                                                                             <  1:880265/150‑1 (MQ=255)
                                             cAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGt                                                                                                        <  1:3844641/150‑1 (MQ=255)
                                              aggaggCCATTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGTATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTa                                                                                                       <  1:3607866/150‑1 (MQ=255)
                                               ggaggCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTAt                                                                                                      >  2:1459532/1‑150 (MQ=255)
                                                   gCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCAt                                                                                                  <  1:1871271/150‑1 (MQ=255)
                                                   gCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCAt                                                                                                  <  1:3928519/150‑1 (MQ=255)
                                                        tATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGtt                                                                                             <  2:3290575/150‑1 (MQ=255)
                                                        tATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGtt                                                                                             <  1:1719687/150‑1 (MQ=255)
                                                        tATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGtt                                                                                             <  1:3525999/150‑1 (MQ=255)
                                                        tATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGtt                                                                                             >  2:3419600/1‑150 (MQ=255)
                                                            aCCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAAt                                                                                         <  2:829714/150‑1 (MQ=255)
                                                            aCCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAAt                                                                                         <  2:363161/150‑1 (MQ=255)
                                                            aCCGTATTACATAGTGGGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCTAAAGAACCCCGGCTCCCGCAGGCGGATATTCCTGAACAACATCATGATTTTCCTGAAATGGTGATTGTCGAACATGGCACGGGTCTTCATGTGTGTAAt                                                                                         >  2:1688074/1‑150 (MQ=255)
                                                               gTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATggg                                                                                      >  1:1143837/1‑150 (MQ=255)
                                                                  ttACATAGTGTGGATTTTTTTACGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTGATGTGTTTAATGGGCAg                                                                                   >  1:1770849/1‑150 (MQ=255)
                                                                     cATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGccc                                                                                <  1:2338948/150‑1 (MQ=255)
                                                                      aTAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCt                                                                               <  1:3966387/150‑1 (MQ=255)
                                                                             ggATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCAt                                                                        >  2:2452409/1‑150 (MQ=255)
                                                                             ggATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCAt                                                                        >  2:236987/1‑150 (MQ=255)
                                                                                 ttttttCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCAcc                                                                    >  1:4119454/1‑150 (MQ=255)
                                                                                 ttttttCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCAcc                                                                    >  2:2420401/1‑150 (MQ=255)
                                                                                    tttCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGt                                                                 <  2:2259945/150‑1 (MQ=255)
                                                                                    tttCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGt                                                                 >  1:2523441/1‑150 (MQ=255)
                                                                                    tttCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGt                                                                 >  2:1959124/1‑150 (MQ=255)
                                                                                      tCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTgg                                                               >  1:3815796/1‑150 (MQ=255)
                                                                                        cGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTTTTTAATGGGCAGCCCTATACCATCACCGGTGGCa                                                             >  1:2554867/1‑150 (MQ=255)
                                                                                         gTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCAc                                                            >  2:2037793/1‑150 (MQ=255)
                                                                                         gTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCAc                                                            >  1:4034411/1‑150 (MQ=255)
                                                                                         gTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCAc                                                            >  1:4034886/1‑150 (MQ=255)
                                                                                           cTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACgg                                                          >  1:1092484/1‑150 (MQ=255)
                                                                                            tGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGt                                                         >  2:3956071/1‑150 (MQ=255)
                                                                                              gTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCt                                                       <  2:2565348/150‑1 (MQ=255)
                                                                                              gTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCt                                                       >  2:183420/1‑150 (MQ=255)
                                                                                               tAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTg                                                      <  2:3682695/150‑1 (MQ=255)
                                                                                                aaCGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGt                                                     >  2:270929/1‑150 (MQ=255)
                                                                                                aaCGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGt                                                     >  1:349607/1‑150 (MQ=255)
                                                                                                aaCGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGt                                                     >  1:2089698/1‑150 (MQ=255)
                                                                                                aaCGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGt                                                     >  2:633072/1‑150 (MQ=255)
                                                                                                  cgcgTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGttt                                                   >  1:4090698/1‑150 (MQ=255)
                                                                                                     gTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGt                                                >  2:1130569/1‑150 (MQ=255)
                                                                                                          tGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTAcgcg                                           <  2:2118295/150‑1 (MQ=255)
                                                                                                           ggCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGa                                          >  1:1348369/1‑150 (MQ=255)
                                                                                                           ggCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGa                                          <  2:2438380/150‑1 (MQ=255)
                                                                                                           ggCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGa                                          <  2:725112/150‑1 (MQ=255)
                                                                                                                 aGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGa                                    <  2:1978023/150‑1 (MQ=255)
                                                                                                                   aaCCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATc                                  >  1:3542989/1‑150 (MQ=255)
                                                                                                                   aaCCCCGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATc                                  >  1:1529838/1‑150 (MQ=255)
                                                                                                                     ccccGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCgg                                <  2:1390432/150‑1 (MQ=255)
                                                                                                                     ccccGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCgg                                <  1:3023183/150‑1 (MQ=255)
                                                                                                                      cccGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGc                               <  2:7435/150‑1 (MQ=255)
                                                                                                                        cGGCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCAt                             >  1:1296737/1‑150 (MQ=255)
                                                                                                                          gCTCCCGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCt                           <  1:1024595/150‑1 (MQ=255)
                                                                                                                           cTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTg                          <  1:373218/150‑1 (MQ=255)
                                                                                                                            tCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGt                         >  1:809847/1‑150 (MQ=255)
                                                                                                                               cGCAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATg                      >  2:3141997/1‑150 (MQ=255)
                                                                                                                                 cAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGaa                    <  1:1673422/150‑1 (MQ=255)
                                                                                                                                 cAGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGaa                    >  2:350125/1‑150 (MQ=255)
                                                                                                                                  aGGCGGATTTTCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAAc                   <  2:2766999/150‑1 (MQ=255)
                                                                                                                                     cGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATa                >  1:677793/1‑150 (MQ=255)
                                                                                                                                          tttCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGAt           <  2:3789250/150‑1 (MQ=255)
                                                                                                                                          tttCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGAt           <  2:3094899/150‑1 (MQ=255)
                                                                                                                                           ttCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATa          <  2:1352219/150‑1 (MQ=255)
                                                                                                                                           ttCCTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATa          <  2:3229993/150‑1 (MQ=255)
                                                                                                                                             ccTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAAt        <  1:2247867/150‑1 (MQ=255)
                                                                                                                                             ccTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGAACGGCATCTGTATGAACATACCGATAAt        >  1:3116992/1‑150 (MQ=255)
                                                                                                                                              cTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCTCGGGTATTCATGTGTTTTATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATc       <  2:2623982/150‑1 (MQ=255)
                                                                                                                                              cTGAAAATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATc       >  2:3385173/1‑150 (MQ=255)
                                                                                                                                              cTGAAAATAATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATc       >  2:2222732/1‑150 (MQ=255)
                                                                                                                                                   catcatcatGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATCTgtgt  <  1:1101378/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAACATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATCACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGT  >  CP009273/4087607‑4087903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A