Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,143,605 G→T 35.9% intergenic (‑579/+19) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,143,6050GT35.9% 283.0 / 193.4 197intergenic (‑579/+19)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (69/57);  new base T (47/24);  total (116/81)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 200 total aligned reads displayed.

AACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGC  >  CP009273/4143458‑4143754
                                                                                                                                                   |                                                                                                                                                     
aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTcc                                                                                                                                                     >  1:4100620/1‑150 (MQ=255)
aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTCGCAACAGAGCCGGATGCGGCGCGAGCTcc                                                                                                                                                     >  2:2157061/1‑150 (MQ=255)
 aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGATTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCt                                                                                                                                                    <  2:2505598/150‑1 (MQ=255)
  cAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCtt                                                                                                                                                   >  2:4039045/1‑150 (MQ=39)
   aGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTa                                                                                                                                                  >  1:3073498/1‑150 (MQ=255)
   aGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                                  >  2:1020455/1‑150 (MQ=39)
            tGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                                         <  2:1491804/150‑1 (MQ=39)
               ggcgCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGt                                                                                                                                      >  1:2131295/1‑150 (MQ=255)
                 cgcgAGCTCCTTATCCGGCCCCCGGTTGGGCCTCGTTTGAGTCTCTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGttt                                                                                                                                    <  2:2491136/150‑1 (MQ=14)
                     aGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAAt                                                                                                                                >  1:1817674/1‑150 (MQ=255)
                     aGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACAGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAAt                                                                                                                                >  1:1005455/1‑150 (MQ=255)
                       cgcCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGc                                                                                                                              >  1:144156/1‑150 (MQ=255)
                             aTCCGGCCTACTGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCaa                                                                                                                        <  2:3455782/150‑1 (MQ=255)
                             aTCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCaa                                                                                                                        >  2:3254607/1‑150 (MQ=255)
                              tCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCaaa                                                                                                                       >  1:545684/1‑150 (MQ=255)
                                  gCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATg                                                                                                                   >  2:752487/1‑150 (MQ=255)
                                  gCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATg                                                                                                                   >  2:3387173/1‑150 (MQ=255)
                                    cTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGt                                                                                                                 >  2:2776587/1‑150 (MQ=255)
                                     tACTGTTGGGCATCGTTTGAGTCTCTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTg                                                                                                                <  2:861327/150‑1 (MQ=255)
                                      ccGGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCACGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGa                                                                                                               <  2:1437835/149‑1 (MQ=255)
                                       cGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGaa                                                                                                              >  1:2576279/1‑150 (MQ=255)
                                           tGGGCATCGTTTGAGTCTCTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTAc                                                                                                          <  2:2798732/150‑1 (MQ=255)
                                             ggCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCt                                                                                                        >  2:2945430/1‑150 (MQ=255)
                                                  cGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGtt                                                                                                   >  1:1829842/1‑150 (MQ=255)
                                                         gTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGtt                                                                                            >  2:1892583/1‑150 (MQ=255)
                                                          tCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTc                                                                                           >  1:3547347/1‑150 (MQ=255)
                                                             gtGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTcgcg                                                                                        <  2:1240613/149‑1 (MQ=255)
                                                             cTGTAGGTGGGTTAAGATGCGCTAGCTTCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTcgcg                                                                                        <  1:103312/150‑1 (MQ=255)
                                                             cTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTcgcg                                                                                        >  1:3562431/1‑150 (MQ=255)
                                                               gTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCggg                                                                                      <  2:2588403/150‑1 (MQ=255)
                                                                tAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGt                                                                                     >  2:4086967/1‑150 (MQ=255)
                                                                 aGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGttt                                                                                                                                    <  2:1355939/102‑1 (MQ=18)
                                                                 aGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTg                                                                                    >  2:3792751/1‑150 (MQ=255)
                                                                  ggTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTgg                                                                                   >  1:2158389/1‑150 (MQ=255)
                                                                  ggTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCACAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTgg                                                                                   >  1:3722672/1‑150 (MQ=255)
                                                                  ggTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTgg                                                                                   >  1:1624137/1‑150 (MQ=255)
                                                                   gTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTggg                                                                                  >  1:4001058/1‑150 (MQ=255)
                                                                   gTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTggg                                                                                  >  1:4000955/1‑150 (MQ=255)
                                                                     cGGATAAGATGCGCAAGGATCGCATCCGACTTTTTTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGTGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCt                                                                                <  2:582939/150‑1 (MQ=255)
                                                                     cGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGTGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCt                                                                                <  1:2660081/150‑1 (MQ=255)
                                                                      ggATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATAACCTGGGTTATCAGTTCGCGGGTGGGCtt                                                                               >  2:1332841/1‑150 (MQ=255)
                                                                       gATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGGTCGAGGGTGGGCTTg                                                                              >  2:223791/1‑150 (MQ=255)
                                                                       gATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTg                                                                              <  1:1921342/150‑1 (MQ=255)
                                                                         tAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGAAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGAt                                                                            >  2:833113/1‑150 (MQ=255)
                                                                          aaGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATa                                                                           >  1:3175782/1‑150 (MQ=255)
                                                                           aGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATaa                                                                          <  1:3398691/150‑1 (MQ=255)
                                                                           aGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATaa                                                                          >  1:4001463/1‑150 (MQ=255)
                                                                             aTGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAAc                                                                        >  1:3243195/1‑150 (MQ=255)
                                                                                  cAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCgtgt                                                                   <  1:1811216/150‑1 (MQ=255)
                                                                                  cAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCgtgt                                                                   >  1:274023/1‑150 (MQ=255)
                                                                                   aaGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGtt                                                                  >  2:636187/1‑150 (MQ=255)
                                                                                    agcagcGCATCCGACATTCTTGCGGCCCTGGTGTTTGGCAACCGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGttt                                                                 <  1:1780678/150‑1 (MQ=37)
                                                                                    aGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGttt                                                                 >  2:415246/1‑150 (MQ=255)
                                                                                     gtatcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTc                                                                <  1:2486017/148‑1 (MQ=255)
                                                                                     gcatcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTc                                                                >  2:792121/1‑150 (MQ=255)
                                                                                     gcatcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTc                                                                <  1:3505254/150‑1 (MQ=255)
                                                                                      tatcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTggg                                                                                                     <  1:82350/111‑1 (MQ=255)
                                                                                      catcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTcc                                                               >  1:2648474/1‑150 (MQ=255)
                                                                                       atcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCa                                                              >  2:878365/1‑150 (MQ=255)
                                                                                       atcgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCa                                                              <  1:3618683/150‑1 (MQ=255)
                                                                                         cgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGa                                                            >  1:89085/1‑150 (MQ=255)
                                                                                         cgcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGa                                                            >  1:3135503/1‑150 (MQ=255)
                                                                                          gcatcCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGat                                                           >  2:1374109/1‑150 (MQ=255)
                                                                                           catcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGata                                                          >  1:2824676/1‑150 (MQ=255)
                                                                                            atcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGatat                                                         >  1:2774909/1‑150 (MQ=255)
                                                                                            atcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGatat                                                         >  1:2774718/1‑150 (MQ=255)
                                                                                            atcCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGatat                                                         >  2:475044/1‑150 (MQ=255)
                                                                                                gACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATtcat                                                     <  2:709246/150‑1 (MQ=255)
                                                                                                 aCATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATtcatc                                                    >  1:3545383/1‑150 (MQ=255)
                                                                                                   attattGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAg                                                  >  1:1062593/1‑150 (MQ=255)
                                                                                                         gCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgat                                            <  1:3107802/150‑1 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCATCAGCGCCGGATGCGGCGCGAGCTCCTTATCCGTCCTACGTTTTACTGCCAGCAAAAATGGTGAATTACCTGGGTTCTCTGTTCGCGGGTTGGATTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           <  1:156686/150‑1 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           <  1:1327156/150‑1 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           <  2:903553/150‑1 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           <  1:1595205/150‑1 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           >  1:4146617/1‑150 (MQ=255)
                                                                                                          cGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTtgatg                                           <  1:239944/150‑1 (MQ=255)
                                                                                                               cTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAAAGACATCAAAAAAGGTGAATTACCTGGGTTATCAGGTCGAGGCTGGTCATTATAAAACGGGTATCCAGAGATTCTTCAGGTTGATGGGcca                                      >  1:425980/1‑149 (MQ=18)
                                                                                                                 ggAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGa                                    >  2:3131400/1‑150 (MQ=255)
                                                                                                                  gAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGAt                                   <  1:11887/150‑1 (MQ=255)
                                                                                                                   aGTTTGGCAACAGTGCCGGAGGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATTCCCGCACAAATGGTGAAGTACCTGGGATATCAGAACGCGGGTGGGCTTGAAAAAAAGAGGGACAAGATATTCGACACGTAGATGAGCCTGagt                                  >  2:1303880/1‑148 (MQ=16)
                                                                                                                    gTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTa                                 <  1:3740604/150‑1 (MQ=255)
                                                                                                                           aaCAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGc                          >  1:1395247/1‑150 (MQ=255)
                                                                                                                           aaCAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATACAACAGGTTGATGAGCCTGATTAATTGAGc                          >  1:3785507/1‑150 (MQ=255)
                                                                                                                             cAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCa                        <  1:1743853/150‑1 (MQ=255)
                                                                                                                              aGCGCCGGATGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAg                       >  1:1508065/1‑150 (MQ=255)
                                                                                                                              aGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAg                       <  1:2158120/150‑1 (MQ=255)
                                                                                                                                cgcCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGc                     >  2:1891785/1‑150 (MQ=255)
                                                                                                                                  ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGccc                   <  1:2914874/150‑1 (MQ=255)
                                                                                                                                   cGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGcccc                  <  2:1046665/150‑1 (MQ=255)
                                                                                                                                   cGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGcccc                  <  2:1134831/150‑1 (MQ=255)
                                                                                                                                      aTGCGGCGCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAac               <  2:733036/150‑1 (MQ=255)
                                                                                                                                        gcggcgCGAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAacac             <  1:3805662/150‑1 (MQ=255)
                                                                                                                                          ggcgCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCa           <  2:425986/150‑1 (MQ=255)
                                                                                                                                           gcgcGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAg          <  2:4121222/150‑1 (MQ=255)
                                                                                                                                             gcgAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCg        <  1:629705/150‑1 (MQ=255)
                                                                                                                                               gAGCTCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTc      >  2:172888/1‑150 (MQ=255)
                                                                                                                                               gAGCTCCATATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTc      >  2:663633/1‑150 (MQ=255)
                                                                                                                                                  cgcCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCggg   <  1:3641325/150‑1 (MQ=255)
                                                                                                                                                   gcCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGc  >  2:251214/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGC  >  CP009273/4143458‑4143754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A