Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 637,005 C→A 20.5% D107Y (GAT→TAT)  uspG ← universal stress protein UP12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273637,0050CA20.5% 745.3 / 134.6 268D107Y (GAT→TAT) uspGuniversal stress protein UP12
Reads supporting (aligned to +/- strand):  ref base C (90/122);  new base A (26/30);  total (116/152)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

GGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGCTCTTCAAAAC  >  CP009273/636856‑637154
                                                                                                                                                     |                                                                                                                                                     
gggATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATc                                                                                                                                                       >  1:1328160/1‑150 (MQ=255)
gggATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATc                                                                                                                                                       >  2:3870562/1‑150 (MQ=255)
 ggATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCa                                                                                                                                                      <  1:1928772/150‑1 (MQ=255)
   aTGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGc                                                                                                                                                    >  2:2373201/1‑150 (MQ=255)
   aTGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGc                                                                                                                                                    >  2:1312473/1‑150 (MQ=255)
       cTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGccccc                                                                                                                                                <  2:1560727/150‑1 (MQ=255)
       cTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGccccc                                                                                                                                                >  1:4696830/1‑150 (MQ=255)
         ccGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAg                                                                                                                                              >  2:4719910/1‑150 (MQ=255)
         ccGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAg                                                                                                                                              >  2:4719896/1‑150 (MQ=255)
              cAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGttctt                                                                                                                                         >  1:116114/1‑150 (MQ=255)
               aGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGttcttc                                                                                                                                        <  2:2155244/150‑1 (MQ=255)
                gggCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTtcttct                                                                                                                                       >  2:2414517/1‑150 (MQ=255)
                gggCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTtcttct                                                                                                                                       >  1:3086615/1‑150 (MQ=255)
                 ggCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTg                                                                                                                                      <  2:3285002/150‑1 (MQ=255)
                   cGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGcc                                                                                                                                    >  2:1940471/1‑150 (MQ=255)
                    gggATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCa                                                                                                                                   >  2:4398260/1‑150 (MQ=255)
                     ggATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCaa                                                                                                                                  <  1:1225584/150‑1 (MQ=255)
                        tACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTc                                                                                                                               <  1:3151082/150‑1 (MQ=255)
                         aCTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCa                                                                                                                              <  1:506728/150‑1 (MQ=255)
                          cTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCAt                                                                                                                             >  1:4065587/1‑150 (MQ=255)
                          cTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCAt                                                                                                                             >  2:502720/1‑150 (MQ=255)
                          cTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCAt                                                                                                                             >  2:4330524/1‑150 (MQ=255)
                           ttATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCAtt                                                                                                                            <  1:3697794/150‑1 (MQ=255)
                           ttATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCAtt                                                                                                                            >  1:1760572/1‑150 (MQ=255)
                                cTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACtt                                                                                                                       >  2:1055658/1‑150 (MQ=255)
                                  ggCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCa                                                                                                                     >  1:226144/1‑150 (MQ=255)
                                      aTTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACACCATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATccc                                                                                                                 <  2:2765734/150‑1 (MQ=255)
                                      aTTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATccc                                                                                                                 <  2:2200063/150‑1 (MQ=255)
                                             cacaACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCt                                                                                                          <  1:2708151/150‑1 (MQ=255)
                                              acaACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTa                                                                                                         <  1:4517988/150‑1 (MQ=255)
                                                aaCCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTAcc                                                                                                       <  1:2428514/150‑1 (MQ=255)
                                                aaCCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTAcc                                                                                                       >  2:1896436/1‑150 (MQ=255)
                                                  ccAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCaa                                                                                                     <  2:3036804/150‑1 (MQ=255)
                                                  ccAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCaa                                                                                                     <  1:4480145/150‑1 (MQ=255)
                                                  ccAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTCAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCaa                                                                                                     <  1:1290188/150‑1 (MQ=255)
                                                     gCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAAc                                                                                                  <  1:3207810/150‑1 (MQ=255)
                                                      cACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACg                                                                                                 <  1:1728961/150‑1 (MQ=255)
                                                        ccGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGa                                                                                               >  2:2701620/1‑150 (MQ=255)
                                                        ccGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGa                                                                                               <  2:3018188/150‑1 (MQ=255)
                                                            cAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACAtg                                                                                           >  1:1387717/1‑150 (MQ=255)
                                                            cAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACAtg                                                                                           <  1:1408452/150‑1 (MQ=255)
                                                               aTTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACAtgttg                                                                                        >  2:4838002/1‑150 (MQ=255)
                                                                ttGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACAtgttgt                                                                                       <  2:3100696/150‑1 (MQ=255)
                                                                 tggcgtggcgGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATgttgtt                                                                                      <  1:4372506/150‑1 (MQ=255)
                                                                 tggcgtggcgGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATgttgtt                                                                                      >  2:2299782/1‑150 (MQ=255)
                                                                   gcgtggcgGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTa                                                                                    <  1:2550048/150‑1 (MQ=255)
                                                                     gtggcgGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAAt                                                                                  >  2:39910/1‑150 (MQ=255)
                                                                          ggATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCggg                                                                             >  1:4823103/1‑150 (MQ=255)
                                                                           gATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGa                                                                            <  1:673562/150‑1 (MQ=255)
                                                                              cACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAgg                                                                         >  1:1970630/1‑150 (MQ=255)
                                                                               aCGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGa                                                                        <  2:4236705/150‑1 (MQ=255)
                                                                                 gCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATc                                                                      <  2:4597530/150‑1 (MQ=255)
                                                                                 gCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATc                                                                      <  2:3160332/150‑1 (MQ=255)
                                                                                 gCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATc                                                                      <  2:2147950/150‑1 (MQ=255)
                                                                                 gCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATc                                                                      <  2:2652390/150‑1 (MQ=255)
                                                                                  cTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCg                                                                     <  1:4398260/150‑1 (MQ=255)
                                                                                   tCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTTAATTCATACCGCACGCAACCAAAACGGACAATTTTTTTAATGCGGGAAGGATCGa                                                                    >  1:2935466/1‑150 (MQ=255)
                                                                                   tCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGa                                                                    >  2:1211398/1‑150 (MQ=255)
                                                                                     gAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATg                                                                  <  2:575704/150‑1 (MQ=255)
                                                                                      aGGCGTTAGAACCTACCAGATGTGTCGAAATCTATGGGTTGCGAGAAACAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATgt                                                                 <  1:2055183/150‑2 (MQ=255)
                                                                                          gTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGaa                                                             >  1:970520/1‑150 (MQ=255)
                                                                                          gTTAGAACCTAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGaa                                                             >  1:3416483/1‑150 (MQ=255)
                                                                                          gTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGaa                                                             >  2:3599219/1‑150 (MQ=255)
                                                                                          gTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGaa                                                             <  1:3714788/150‑1 (MQ=255)
                                                                                           ttAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAg                                                            <  1:760569/150‑1 (MQ=255)
                                                                                            tAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGt                                                           >  1:410944/1‑150 (MQ=255)
                                                                                                   tAACAGATGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGAc                                                    >  1:32899/1‑150 (MQ=255)
                                                                                                   tAACAGAGGGGGCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGAc                                                    <  2:4213429/150‑1 (MQ=255)
                                                                                                      cAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCAt                                                 <  2:404115/150‑1 (MQ=255)
                                                                                                          tGGGTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGtt                                             <  1:3267264/150‑1 (MQ=255)
                                                                                                          tGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGtt                                             >  1:1948506/1‑150 (MQ=255)
                                                                                                             gTCGAAATCTATGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGc                                          >  1:4157726/1‑150 (MQ=255)
                                                                                                                    tCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGtt                                   <  1:4386880/150‑1 (MQ=255)
                                                                                                                     ctaTGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTc                                  <  2:1770428/148‑1 (MQ=255)
                                                                                                                      gATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCt                                 >  2:920391/1‑150 (MQ=255)
                                                                                                                      gATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCt                                 <  2:342762/150‑1 (MQ=255)
                                                                                                                       aTGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCtt                                <  1:2679255/150‑1 (MQ=255)
                                                                                                                       aTGGGTTGCGAGAACCAAGAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCtt                                <  2:639947/150‑1 (MQ=255)
                                                                                                                        tGGGTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTtg                               <  1:1312473/150‑1 (MQ=255)
                                                                                                                         gggTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTtgt                              >  1:805824/1‑150 (MQ=255)
                                                                                                                         gggTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTtgt                              <  1:1993528/150‑1 (MQ=255)
                                                                                                                         gggTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTtgt                              >  1:1525682/1‑150 (MQ=255)
                                                                                                                          ggTTGCGAGAACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTtgtg                             <  1:1041678/150‑1 (MQ=255)
                                                                                                                               cGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCa                        <  1:2151311/150‑1 (MQ=255)
                                                                                                                                gagaACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCAt                       >  2:3244562/1‑150 (MQ=255)
                                                                                                                                  gaACCAATAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGt                     <  2:1754965/150‑1 (MQ=255)
                                                                                                                                     ccAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGc                  <  1:954144/150‑1 (MQ=255)
                                                                                                                                      cAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCa                 >  1:1013402/1‑150 (MQ=255)
                                                                                                                                         tAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGAt              <  1:2565814/150‑1 (MQ=255)
                                                                                                                                         tAACTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGAt              <  2:4674692/150‑1 (MQ=255)
                                                                                                                                          aaCTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATg             <  1:3870562/150‑1 (MQ=255)
                                                                                                                                          aaCTACAACATAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATg             >  2:4510906/1‑150 (MQ=255)
                                                                                                                                           aCTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGc            <  1:4612611/150‑1 (MQ=255)
                                                                                                                                            cTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGct           <  1:3572474/150‑1 (MQ=255)
                                                                                                                                            cTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGct           <  1:4330524/150‑1 (MQ=255)
                                                                                                                                              acaacaTCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGctct         <  2:1387717/150‑1 (MQ=255)
                                                                                                                                              acaacaTCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGctct         >  2:2567946/1‑150 (MQ=255)
                                                                                                                                                aacaTAAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGCTCTTc       >  1:3658120/1‑150 (MQ=255)
                                                                                                                                                 acaTCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGCTCTTCa      <  2:1667148/150‑1 (MQ=255)
                                                                                                                                                 acaTAAGCCCCCAGTTCTTCTGCCAACTCCTTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGCTCTTCa      <  1:1223823/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTCGAAATCGATGGGTTGCGAGAACCAATAACTACAACATCAGCCCCCAGTTCTTCTGCCAACTCATTGACTTCATCCCGCACGCTACCAAAACGGACATGTTGTTTAATGCGGGAAGGATCGATGGTGAAGTGGCTGACCATCGTTTGCAGACGTTCTTGTGCTTCATGTTGCAGATGCTCTTCAAAAC  >  CP009273/636856‑637154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A