Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,706,734 C→T 20.7% intergenic (+319/‑292) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,706,7340CT20.7% 310.6 / 75.6 135intergenic (+319/‑292)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (64/43);  new base T (17/11);  total (81/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 136 total aligned reads displayed.

TTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATT  >  CP009273/1706585‑1706882
                                                                                                                                                     |                                                                                                                                                     
ttcGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAAc                                                                                                                                                       <  1:1059268/150‑1 (MQ=255)
    tGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTa                                                                                                                                                   <  2:1706728/150‑1 (MQ=255)
      aTCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTaaa                                                                                                                                                 >  1:103727/1‑150 (MQ=255)
      aTCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTaaa                                                                                                                                                 >  1:3842660/1‑150 (MQ=255)
      aTCAAGATCACGAAGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTaaa                                                                                                                                                 <  1:4568563/150‑1 (MQ=255)
        cAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATa                                                                                                                                               <  2:2433531/150‑1 (MQ=255)
         aaGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAg                                                                                                                                              <  2:1726223/150‑1 (MQ=255)
         aaGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAg                                                                                                                                              >  2:3594909/1‑150 (MQ=255)
            aTCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAg                                                                                                                                           >  2:3039747/1‑150 (MQ=255)
            aTCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAg                                                                                                                                           >  2:3244154/1‑150 (MQ=255)
                cGATGCTGTCGCAATGTAAAAACATTGCAAGTATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACa                                                                                                                                       >  1:1016723/1‑150 (MQ=255)
                    gCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATc                                                                                                                                   >  1:2255915/1‑150 (MQ=255)
                          gCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGtt                                                                                                                             <  2:3559313/150‑1 (MQ=255)
                          gCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGtt                                                                                                                             <  2:51413/150‑1 (MQ=255)
                            aaTGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTaa                                                                                                                           >  2:3787747/1‑150 (MQ=255)
                             aTGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAg                                                                                                                          <  2:4431415/150‑1 (MQ=255)
                              tGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGc                                                                                                                         <  1:4027472/150‑1 (MQ=255)
                               gTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGcc                                                                                                                        <  1:1890928/150‑1 (MQ=255)
                                tAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCa                                                                                                                       >  1:4353415/1‑150 (MQ=255)
                                tAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCa                                                                                                                       <  1:674890/150‑1 (MQ=255)
                                 aaaaaCATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCAt                                                                                                                      <  2:195214/150‑1 (MQ=255)
                                   aaaCATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCa                                                                                                                    <  1:1415823/150‑1 (MQ=255)
                                    aaCATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAc                                                                                                                   >  2:1262447/1‑150 (MQ=255)
                                       aTTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcccc                                                                                                                <  2:1670166/150‑1 (MQ=255)
                                              gAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTca                                                                                                         <  1:1220926/150‑1 (MQ=255)
                                              gAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTca                                                                                                         >  2:3242979/1‑150 (MQ=255)
                                                aTCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTcagt                                                                                                       >  2:4257013/1‑150 (MQ=255)
                                                 tCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTcagtg                                                                                                      <  1:4714516/150‑1 (MQ=255)
                                                  cAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTcagtgc                                                                                                     >  2:3945424/1‑150 (MQ=255)
                                                       tAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑a                                                                                               >  1:3560801/1‑150 (MQ=255)
                                                       tAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑a                                                                                               >  1:3560797/1‑150 (MQ=255)
                                                       tAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑a                                                                                               <  2:4575927/150‑1 (MQ=255)
                                                       tAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑a                                                                                               >  1:4520902/1‑150 (MQ=255)
                                                       tAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑a                                                                                               >  1:4520898/1‑150 (MQ=255)
                                                        aaaaTCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑aa                                                                                              >  1:3304685/1‑150 (MQ=255)
                                                        aaaaTCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑aa                                                                                              >  1:1927111/1‑150 (MQ=255)
                                                           aTCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑aaaaa                                                                                           >  1:3961381/1‑150 (MQ=255)
                                                             cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAAt                                                                                         <  2:552311/150‑1 (MQ=255)
                                                             cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAAt                                                                                         <  2:1986534/150‑1 (MQ=255)
                                                             cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAAt                                                                                         >  2:3482273/1‑150 (MQ=255)
                                                             cATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAAt                                                                                         <  2:3900242/150‑1 (MQ=255)
                                                              aTTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATc                                                                                        >  2:2678493/1‑150 (MQ=255)
                                                                   aaTTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCg                                                                                   <  1:1856852/150‑1 (MQ=255)
                                                                    aTTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGt                                                                                  <  1:3636111/150‑1 (MQ=255)
                                                                      ttACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTa                                                                                >  1:3686240/1‑150 (MQ=255)
                                                                      ttACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTa                                                                                >  1:3686235/1‑150 (MQ=255)
                                                                       tACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTAc                                                                               >  1:1280026/1‑150 (MQ=255)
                                                                        acacacGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAAATCTGCCGTTAc                                                                               >  2:2329396/1‑150 (MQ=255)
                                                                         cacacGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGt                                                                             <  1:3242951/150‑1 (MQ=255)
                                                                          acacGCAACAAATATTGACCTACAAAACATTACTCTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAAAATATCTCCGTTAAGCCATCACCCCCCTATAAGTTCAGTG‑AAAAAATCTGCCGTTACGtt                                                                            <  2:2294052/150‑1 (MQ=255)
                                                                          acacGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGtt                                                                            <  2:2293324/150‑1 (MQ=255)
                                                                             cGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGttttt                                                                         >  2:1008101/1‑150 (MQ=255)
                                                                             cGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGttttt                                                                         <  1:3875509/150‑1 (MQ=255)
                                                                              gCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGtttttt                                                                        <  1:2477468/150‑1 (MQ=255)
                                                                                  caaATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGaaa                                                                    <  2:3577959/150‑1 (MQ=255)
                                                                                  caaATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGaaa                                                                    <  2:3577965/150‑1 (MQ=255)
                                                                                      tatTGACCTACAAAACATTACACTGTCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                                                <  1:825010/150‑1 (MQ=255)
                                                                                      tatTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                                                >  2:3316109/1‑150 (MQ=255)
                                                                                      tatTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                                                <  1:3253928/150‑1 (MQ=255)
                                                                                         tGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                                             >  2:1970716/1‑150 (MQ=255)
                                                                                           aCCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                           >  2:3691319/1‑150 (MQ=255)
                                                                                           aCCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACg                                                           >  2:1228182/1‑150 (MQ=255)
                                                                                              tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                                        <  2:1873047/150‑1 (MQ=255)
                                                                                                cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGa                                                      >  1:918743/1‑150 (MQ=255)
                                                                                                 aaaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGAt                                                     >  2:2266017/1‑150 (MQ=255)
                                                                                                 aaaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGAt                                                     >  1:2377954/1‑150 (MQ=255)
                                                                                                  aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATa                                                    >  2:179365/1‑150 (MQ=255)
                                                                                                  aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATa                                                    >  1:3991431/1‑150 (MQ=255)
                                                                                                     cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCATAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAAGCTGGATaaaa                                                 >  1:1626183/1‑148 (MQ=255)
                                                                                                     cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                                 >  2:4558777/1‑150 (MQ=255)
                                                                                                     cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                                 >  2:3569113/1‑150 (MQ=255)
                                                                                                      aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAACAATTTAACGCTGGATAACAt                                                >  1:1878298/1‑150 (MQ=255)
                                                                                                      aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                                                >  2:435394/1‑150 (MQ=255)
                                                                                                          cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                                            >  2:4814124/1‑150 (MQ=255)
                                                                                                           acTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTccc                                           >  1:2381517/1‑150 (MQ=255)
                                                                                                            cTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTccag                                          >  1:2483678/1‑148 (MQ=255)
                                                                                                                cTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGact                                      >  1:1835465/1‑148 (MQ=255)
                                                                                                                 tATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATg                                     >  2:36305/1‑150 (MQ=255)
                                                                                                                  aTTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGACTgg                                    >  2:3990113/1‑150 (MQ=255)
                                                                                                                  aTTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATgg                                    >  2:3989882/1‑150 (MQ=255)
                                                                                                                    ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATgggt                                  <  2:2070886/150‑3 (MQ=255)
                                                                                                                      ttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa                                >  2:1570386/1‑150 (MQ=255)
                                                                                                                      ttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa                                >  2:1252661/1‑150 (MQ=255)
                                                                                                                        cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAt                              <  2:2920866/150‑1 (MQ=255)
                                                                                                                         aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAtt                             >  1:4233639/1‑150 (MQ=255)
                                                                                                                         aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAtt                             >  1:1921635/1‑150 (MQ=255)
                                                                                                                         aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAtt                             >  1:170292/1‑150 (MQ=255)
                                                                                                                         aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAtt                             >  1:1924208/1‑150 (MQ=255)
                                                                                                                          gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTc                            >  2:1209067/1‑150 (MQ=255)
                                                                                                                           aaaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGACTGGTTGAATTcc                           >  1:1897901/1‑150 (MQ=255)
                                                                                                                           aaaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGAAAACATTTCCCGGAATGGTTGAATTcc                           >  1:1897335/1‑150 (MQ=255)
                                                                                                                             aCTGGACTATCTCACTAGTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcccc                         >  1:109540/1‑150 (MQ=255)
                                                                                                                             aCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcccc                         <  2:1778331/150‑1 (MQ=255)
                                                                                                                              cTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCg                        >  1:2084233/1‑150 (MQ=255)
                                                                                                                              cTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCg                        >  1:2084254/1‑150 (MQ=255)
                                                                                                                                       ctcACTATTAAAAATGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTta               <  2:1448705/150‑1 (MQ=255)
                                                                                                                                               tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAAc       >  1:3126765/1‑150 (MQ=255)
                                                                                                                                               tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAAc       <  1:3787747/150‑1 (MQ=255)
                                                                                                                                                   aaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTACCCGCCTCAGTTATATGTAACAGat   >  2:3277350/1‑150 (MQ=255)
                                                                                                                                                    aCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGatt  <  2:4451464/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTG‑AAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATT  >  CP009273/1706585‑1706882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A