Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,802,047 G→A 74.5% intergenic (+100/‑6) ydiZ → / → yniA uncharacterized protein/fructosamine kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,802,0470GA74.5% 424.6 / 170.0 227intergenic (+100/‑6)ydiZ/yniAuncharacterized protein/fructosamine kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (31/27);  new base A (100/69);  total (131/96)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

AAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAA  >  CP009273/1801901‑1802196
                                                                                                                                                  |                                                                                                                                                     
aaaTCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGAca                                                                                                                                                    <  1:2485456/150‑1 (MQ=255)
 aaTCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGAcat                                                                                                                                                   <  1:3499639/150‑1 (MQ=255)
  aTCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAAcatc                                                                                                                                                  >  1:832408/1‑150 (MQ=255)
      ccATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCAtgt                                                                                                                                              >  1:3281242/1‑150 (MQ=255)
      ccATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCAtgt                                                                                                                                              <  1:2359542/150‑1 (MQ=255)
      ccATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCAtgt                                                                                                                                              >  1:392045/1‑150 (MQ=255)
          cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTggca                                                                                                                                          >  1:2064516/1‑150 (MQ=255)
          cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggca                                                                                                                                          >  1:3961993/1‑150 (MQ=255)
           aCGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggcag                                                                                                                                         <  2:549061/150‑1 (MQ=255)
           aCGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggcag                                                                                                                                         >  2:511808/1‑150 (MQ=255)
                 tGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATc                                                                                                                                   >  2:3008516/1‑150 (MQ=255)
                   ggaggaATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAg                                                                                                                                 <  2:446368/150‑1 (MQ=255)
                          tggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCtt                                                                                                                          <  1:495993/150‑1 (MQ=255)
                          tggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCtt                                                                                                                          >  1:596776/1‑150 (MQ=255)
                             aatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTg                                                                                                                       >  1:3302528/1‑150 (MQ=255)
                             aatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTg                                                                                                                       >  2:899307/1‑150 (MQ=255)
                               tAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTgag                                                                                                                     <  2:1138493/150‑1 (MQ=255)
                               tAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTgag                                                                                                                     >  2:3703372/1‑150 (MQ=255)
                               tAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgag                                                                                                                     >  2:94498/1‑150 (MQ=255)
                                 gCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcg                                                                                                                   <  1:3522048/150‑1 (MQ=255)
                                   aaCAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgag                                                                                                                 <  2:1032320/150‑1 (MQ=255)
                                   aaCAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgag                                                                                                                 >  2:343116/1‑150 (MQ=255)
                                   aaCAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgag                                                                                                                 >  2:1486237/1‑150 (MQ=255)
                                    aCAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgagc                                                                                                                >  1:2979936/1‑150 (MQ=255)
                                      aGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAg                                                                                                              >  1:1809616/1‑150 (MQ=255)
                                      aGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAg                                                                                                              >  2:2713550/1‑150 (MQ=255)
                                        aaaaTAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGtt                                                                                                            <  1:2970303/150‑1 (MQ=255)
                                        aaaaTAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGtt                                                                                                            >  2:1640870/1‑150 (MQ=255)
                                         aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                           <  2:3196905/150‑1 (MQ=255)
                                         aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                           >  2:3099490/1‑150 (MQ=255)
                                         aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                           <  2:3234634/150‑1 (MQ=255)
                                         aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                           <  2:2886011/150‑1 (MQ=255)
                                          aataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAg                                                                                                          >  2:683074/1‑150 (MQ=255)
                                          aataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAg                                                                                                          >  2:1945914/1‑150 (MQ=255)
                                           ataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGTCAGTCAATCAGTCGTCTTTTGAGCGAGCAGTTAgg                                                                                                         >  2:128084/1‑150 (MQ=255)
                                             aaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTg                                                                                                       <  2:119389/150‑1 (MQ=255)
                                              aatGGTTTTTTGGGAATTATCATTCTTTTGGGTGGGTTTTGTCGTTTTTTTACACCGCATTCTTGGGGTTAACTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGa                                                                                                      <  2:4543241/150‑1 (MQ=255)
                                               atGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGaa                                                                                                     >  2:2848691/1‑150 (MQ=255)
                                                 ggTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAgg                                                                                                   >  1:3174519/1‑150 (MQ=255)
                                                  gTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGc                                                                                                  <  2:216204/150‑1 (MQ=255)
                                                       tGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAAt                                                                                             >  2:2252521/1‑150 (MQ=255)
                                                       tGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAAt                                                                                             >  2:1449908/1‑150 (MQ=255)
                                                        gggCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATc                                                                                            <  2:1195081/150‑1 (MQ=255)
                                                          gCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGa                                                                                          >  1:1290711/1‑150 (MQ=255)
                                                           cAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGaa                                                                                         >  2:4531397/1‑150 (MQ=255)
                                                             ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACt                                                                                       >  1:2763545/1‑150 (MQ=255)
                                                             ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACt                                                                                       >  2:2351141/1‑150 (MQ=255)
                                                             ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACt                                                                                       >  1:161545/1‑150 (MQ=255)
                                                             ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCCAAATCGAACt                                                                                       >  1:162115/1‑150 (MQ=255)
                                                               aatCAGTCTGTGGTGTGCGTTAGATCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGTGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCTGTTAGGTGAAGTCGAAATCTAAATga                                                                                     >  2:4708547/1‑149 (MQ=255)
                                                                  cAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTa                                                                                  <  1:4104997/150‑1 (MQ=255)
                                                                    gTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAAt                                                                                <  2:3963223/150‑1 (MQ=255)
                                                                     tCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATg                                                                               >  1:1910982/1‑150 (MQ=255)
                                                                      cTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGa                                                                              <  1:1118621/150‑1 (MQ=255)
                                                                      cTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAAATGCGTAATGa                                                                              >  1:161367/1‑150 (MQ=255)
                                                                           gtgtGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGc                                                                         <  2:4544374/150‑1 (MQ=255)
                                                                              tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTg                                                                      >  1:1733313/1‑150 (MQ=255)
                                                                                cGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGc                                                                    <  2:1426595/150‑1 (MQ=255)
                                                                                 gTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCg                                                                   <  2:4154430/150‑1 (MQ=255)
                                                                                 gTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCg                                                                   <  2:567285/150‑1 (MQ=255)
                                                                                  ttAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCgg                                                                  <  1:2762544/150‑1 (MQ=255)
                                                                                  ttAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCgg                                                                  >  2:3761253/1‑150 (MQ=255)
                                                                                   tAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGga                                                                 >  1:93755/1‑150 (MQ=255)
                                                                                    aGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGgag                                                                <  1:4500178/150‑1 (MQ=255)
                                                                                       tCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAg                                                             >  1:4833936/1‑150 (MQ=255)
                                                                                        cGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGt                                                            <  1:1355247/150‑1 (MQ=255)
                                                                                         gtgtTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTc                                                           >  2:1853224/1‑150 (MQ=255)
                                                                                               ttACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGcc                                                     >  1:3035914/1‑150 (MQ=255)
                                                                                                        aTTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCAt                                            <  1:3753190/150‑1 (MQ=255)
                                                                                                         ttCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCAtt                                           <  1:3799407/150‑1 (MQ=255)
                                                                                                         ttCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCAtt                                           <  1:4620638/150‑1 (MQ=255)
                                                                                                          tCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCAttt                                          >  2:3190358/1‑150 (MQ=255)
                                                                                                           cTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTg                                         >  1:253254/1‑150 (MQ=255)
                                                                                                                gcTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTa                                    >  1:1972243/1‑150 (MQ=255)
                                                                                                                gcTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTa                                    >  2:24780/1‑150 (MQ=255)
                                                                                                                   aaCCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGc                                 <  2:3118581/150‑1 (MQ=255)
                                                                                                                     ccTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAg                               >  2:4472944/1‑150 (MQ=255)
                                                                                                                     ccTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGAAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAg                               >  1:3849798/1‑150 (MQ=255)
                                                                                                                      cTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAgg                              >  2:3077673/1‑150 (MQ=255)
                                                                                                                       ttATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGa                             >  2:4297917/1‑150 (MQ=255)
                                                                                                                        tATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGAc                            >  2:4284723/1‑150 (MQ=255)
                                                                                                                         aTGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACa                           <  2:3532407/150‑1 (MQ=255)
                                                                                                                                 gCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                   <  2:395005/150‑1 (MQ=255)
                                                                                                                                 gCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                   <  2:392045/150‑1 (MQ=255)
                                                                                                                                 gCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                   <  1:1792185/150‑1 (MQ=255)
                                                                                                                                 gCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                   >  2:181113/1‑150 (MQ=255)
                                                                                                                                 gCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                   <  2:3281242/150‑1 (MQ=255)
                                                                                                                                  catacaacaTGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAtttttt                  >  1:4183969/4‑150 (MQ=255)
                                                                                                                                  cAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAtttttt                  >  2:424531/1‑150 (MQ=255)
                                                                                                                                   aGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTc                 <  1:1545219/150‑1 (MQ=255)
                                                                                                                                   aGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTc                 <  1:2598552/150‑1 (MQ=255)
                                                                                                                                   aGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTc                 >  1:3999016/1‑150 (MQ=255)
                                                                                                                                       aacaTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa             >  2:3826176/1‑150 (MQ=255)
                                                                                                                                        acaTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCaa            >  2:3839988/1‑150 (MQ=255)
                                                                                                                                         caTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTAGTCaaa           >  1:3347866/1‑150 (MQ=255)
                                                                                                                                          aTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAt          <  1:652216/150‑1 (MQ=255)
                                                                                                                                          aTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAt          <  2:161367/150‑1 (MQ=255)
                                                                                                                                           tGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAtg         <  1:273654/150‑1 (MQ=255)
                                                                                                                                             ggAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAtgtg       <  1:3324959/150‑1 (MQ=255)
                                                                                                                                               agaaaCATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGtgat     >  1:4042217/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAA  >  CP009273/1801901‑1802196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  CP009273/1801899‑1802195
                                                                                                                                                    |                                                                                                                                                    
TGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAA                                                                                                                                                     <  SRR19973081.1561432/150‑1 (MQ=60)
  AAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACA                                                                                                                                                   <  SRR19973081.2500917/150‑1 (MQ=60)
   AATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACAT                                                                                                                                                  <  SRR19973081.3521090/150‑1 (MQ=60)
   AATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACAT                                                                                                                                                  >  SRR19973081.1625369/1‑150 (MQ=60)
    ATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATC                                                                                                                                                 >  SRR19973081.837692/1‑150 (MQ=60)
        CCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGT                                                                                                                                             >  SRR19973081.3301195/1‑150 (MQ=60)
        CCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGT                                                                                                                                             >  SRR19973081.3301234/1‑150 (MQ=60)
        CCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGT                                                                                                                                             >  SRR19973081.394578/1‑150 (MQ=60)
        CCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGT                                                                                                                                             >  SRR19973081.397553/1‑150 (MQ=60)
        CCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGT                                                                                                                                             <  SRR19973081.2374207/150‑1 (MQ=60)
            CACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCA                                                                                                                                         >  SRR19973081.2077578/1‑150 (MQ=60)
            CACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCA                                                                                                                                         >  SRR19973081.1716516/1‑150 (MQ=60)
            CACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCA                                                                                                                                         >  SRR19973081.3986213/1‑150 (MQ=60)
            CACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCA                                                                                                                                         >  SRR19973081.3987446/1‑150 (MQ=60)
                           ATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCT                                                                                                                          >  SRR19973081.186640/1‑150 (MQ=60)
                            TGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTT                                                                                                                         <  SRR19973081.1836788/150‑1 (MQ=60)
                            TGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTT                                                                                                                         >  SRR19973081.602855/1‑150 (MQ=60)
                            TGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTT                                                                                                                         <  SRR19973081.499528/150‑1 (MQ=60)
                            TGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTT                                                                                                                         >  SRR19973081.600976/1‑150 (MQ=60)
                             GGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTAAACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCTGGCAATCAGTCGTCTTG                                                                                                                        >  SRR19973081.3507534/1‑150 (MQ=60)
                               AATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTG                                                                                                                      >  SRR19973081.2459258/1‑150 (MQ=60)
                               AATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTG                                                                                                                      >  SRR19973081.3322690/1‑150 (MQ=60)
                                   GCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCG                                                                                                                  <  SRR19973081.3543187/150‑1 (MQ=60)
                                   GCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCG                                                                                                                  <  SRR19973081.3543590/150‑1 (MQ=60)
                                      ACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGC                                                                                                               >  SRR19973081.2998296/1‑150 (MQ=60)
                                      ACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGC                                                                                                               >  SRR19973081.2998229/1‑150 (MQ=60)
                                        AGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAG                                                                                                             >  SRR19973081.1821417/1‑150 (MQ=60)
                                        AGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAG                                                                                                             >  SRR19973081.1820712/1‑150 (MQ=60)
                                        AGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAG                                                                                                             <  SRR19973081.2021486/150‑1 (MQ=60)
                                          AAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTT                                                                                                           <  SRR19973081.2988601/150‑1 (MQ=60)
                                             ATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGG                                                                                                        >  SRR19973081.89807/1‑150 (MQ=60)
                                              TAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGT                                                                                                       >  SRR19973081.734140/1‑150 (MQ=60)
                                                  TGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAG                                                                                                   <  SRR19973081.2264533/150‑1 (MQ=60)
                                                   GGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGG                                                                                                  >  SRR19973081.3193828/1‑150 (MQ=60)
                                                         TGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAAT                                                                                            <  SRR19973081.3605010/150‑1 (MQ=60)
                                                            GCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGA                                                                                         <  SRR19973081.324927/150‑1 (MQ=60)
                                                            GCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGA                                                                                         >  SRR19973081.1299286/1‑150 (MQ=60)
                                                               ATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACT                                                                                      >  SRR19973081.2780467/1‑150 (MQ=60)
                                                               ATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACT                                                                                      >  SRR19973081.2780503/1‑150 (MQ=60)
                                                               ATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACT                                                                                      >  SRR19973081.163031/1‑150 (MQ=60)
                                                               ATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCCAAATCGAACT                                                                                      >  SRR19973081.163603/1‑150 (MQ=60)
                                                                 AATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGC                                                                                    >  SRR19973081.591203/1‑150 (MQ=60)
                                                                    CAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTA                                                                                 <  SRR19973081.4129976/150‑1 (MQ=60)
                                                                    CAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTA                                                                                 <  SRR19973081.4128940/150‑1 (MQ=60)
                                                                       TCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATG                                                                              >  SRR19973081.1923200/1‑150 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGA                                                                             <  SRR19973081.4144222/150‑1 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGA                                                                             <  SRR19973081.1125943/150‑1 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGA                                                                             <  SRR19973081.217251/150‑1 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGA                                                                             >  SRR19973081.163909/1‑150 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGA                                                                             <  SRR19973081.1630073/150‑1 (MQ=60)
                                                                        CTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAAATGCGTAATGA                                                                             >  SRR19973081.162852/1‑150 (MQ=60)
                                                                                TGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTG                                                                     <  SRR19973081.158734/150‑1 (MQ=60)
                                                                                TGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTG                                                                     >  SRR19973081.1744690/1‑150 (MQ=60)
                                                                                 GCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGG                                                                    >  SRR19973081.4875260/1‑150 (MQ=60)
                                                                                 GCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGG                                                                    >  SRR19973081.4876397/1‑150 (MQ=60)
                                                                                   GTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCG                                                                  >  SRR19973081.3713400/1‑150 (MQ=60)
                                                                                    TTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGG                                                                 <  SRR19973081.12902/150‑1 (MQ=60)
                                                                                    TTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGG                                                                 <  SRR19973081.2779500/150‑1 (MQ=60)
                                                                                    TTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGG                                                                 <  SRR19973081.2779235/150‑1 (MQ=60)
                                                                                     TAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGA                                                                >  SRR19973081.94754/1‑150 (MQ=60)
                                                                                      AGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAG                                                               <  SRR19973081.4527346/150‑1 (MQ=60)
                                                                                      AGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAG                                                               <  SRR19973081.1329887/150‑1 (MQ=60)
                                                                                         TCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAG                                                            <  SRR19973081.2925854/150‑1 (MQ=60)
                                                                                         TCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAG                                                            >  SRR19973081.4862992/1‑150 (MQ=60)
                                                                                          CGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGT                                                           <  SRR19973081.1364335/150‑1 (MQ=60)
                                                                                                 TTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCC                                                    >  SRR19973081.3054618/1‑150 (MQ=60)
                                                                                                   ACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGC                                                  >  SRR19973081.3015474/1‑150 (MQ=60)
                                                                                                     ACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCAT                                                <  SRR19973081.1557861/150‑1 (MQ=60)
                                                                                                         CATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCA                                            >  SRR19973081.2525113/1‑150 (MQ=60)
                                                                                                         CATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCA                                            >  SRR19973081.3126991/1‑150 (MQ=60)
                                                                                                         CATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCA                                            >  SRR19973081.4858666/1‑150 (MQ=60)
                                                                                                         CATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCA                                            >  SRR19973081.3788075/1‑150 (MQ=60)
                                                                                                          ATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCAT                                           <  SRR19973081.3776191/150‑1 (MQ=60)
                                                                                                           TTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATT                                          <  SRR19973081.3822756/150‑1 (MQ=60)
                                                                                                           TTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATT                                          <  SRR19973081.4648692/150‑1 (MQ=60)
                                                                                                           TTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATT                                          <  SRR19973081.3930553/150‑1 (MQ=60)
                                                                                                             CTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTG                                        >  SRR19973081.255314/1‑150 (MQ=60)
                                                                                                               TGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCG                                      <  SRR19973081.1958243/150‑1 (MQ=60)
                                                                                                                 CGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCT                                    <  SRR19973081.1782738/150‑1 (MQ=60)
                                                                                                                 CGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCT                                    <  SRR19973081.3857558/150‑1 (MQ=60)
                                                                                                                  GCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTA                                   >  SRR19973081.1984648/1‑150 (MQ=60)
                                                                                                                     AACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGC                                <  SRR19973081.839426/150‑1 (MQ=60)
                                                                                                                       CCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGAAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAG                              >  SRR19973081.3873449/1‑150 (MQ=60)
                                                                                                                         TTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGA                            <  SRR19973081.2730322/150‑1 (MQ=60)
                                                                                                                                 TGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTT                    >  SRR19973081.2726713/1‑150 (MQ=60)
                                                                                                                                 TGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTT                    >  SRR19973081.955821/1‑150 (MQ=60)
                                                                                                                                  GGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTT                   <  SRR19973081.4569645/150‑1 (MQ=60)
                                                                                                                                  GGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTT                   >  SRR19973081.2612444/1‑150 (MQ=60)
                                                                                                                                   GCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTT                  <  SRR19973081.1803904/150‑1 (MQ=60)
                                                                                                                                   GCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTT                  <  SRR19973081.3118499/150‑1 (MQ=60)
                                                                                                                                    CATACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTT                 >  SRR19973081.4209548/1‑150 (MQ=60)
                                                                                                                                    CAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTT                 >  SRR19973081.4209865/1‑150 (MQ=60)
                                                                                                                                     AGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTC                <  SRR19973081.1555282/150‑1 (MQ=60)
                                                                                                                                     AGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTC                >  SRR19973081.4023334/1‑150 (MQ=60)
                                                                                                                                     AGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTC                <  SRR19973081.2614740/150‑1 (MQ=60)
                                                                                                                                     AGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTC                >  SRR19973081.3908617/1‑150 (MQ=60)
                                                                                                                                     AGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTAGCGCTATGCAGGACATGATTTTATC                >  SRR19973081.1550160/1‑150 (MQ=60)
                                                                                                                                           CATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAA          >  SRR19973081.4036457/1‑150 (MQ=60)
                                                                                                                                           CATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAA          >  SRR19973081.4433663/1‑150 (MQ=60)
                                                                                                                                           CATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAA          <  SRR19973081.2968009/150‑1 (MQ=60)
                                                                                                                                           CATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAA          >  SRR19973081.3368097/1‑150 (MQ=60)
                                                                                                                                           CATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTAGTCAAA          >  SRR19973081.3368405/1‑150 (MQ=60)
                                                                                                                                            ATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAT         <  SRR19973081.656687/150‑1 (MQ=60)
                                                                                                                                             TGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATG        <  SRR19973081.275804/150‑1 (MQ=60)
                                                                                                                                               TGAGAAACATCATGTGTCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAATTCGAAATCTAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCTGGACATGATTTTTTCGTCAACTGTG      <  SRR19973081.3345293/150‑1 (MQ=60)
                                                                                                                                               GGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTG      <  SRR19973081.3345319/150‑1 (MQ=60)
                                                                                                                                               GGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTG      <  SRR19973081.3345040/150‑1 (MQ=60)
                                                                                                                                                 AGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCTTAATGAAATGACTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATTCAGGACATTATTTTTTCGTCAAATGTTAT    >  SRR19973081.4066620/1‑150 (MQ=60)
                                                                                                                                                 AGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGAT    >  SRR19973081.4066652/1‑150 (MQ=60)
                                                                                                                                                   AAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  SRR19973081.4771710/1‑150 (MQ=60)
                                                                                                                                                   AAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  SRR19973081.4771619/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  CP009273/1801899‑1802195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑